公开(公告)号：AU2007237478A1

公开(公告)日：2007-10-25

申请号：AU2007237478

申请日：2007-04-11

Applicant: HOFFMANN LA ROCHE

Inventor： ELTZ HERBERT VON DER ,  KRATZSCH PETER ,  SCHMUCK RAINER ,  BOENITZ-DULAT MARA ,  BECK DANIELA

IPC: C12N9/04

Abstract: The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.

公开(公告)号：ES2803273T3

公开(公告)日：2021-01-25

申请号：ES16826037

申请日：2016-12-21

Applicant: HOFFMANN LA ROCHE

Inventor： KRATZSCH PETER ,  BOENITZ-DULAT MARA ,  BECK DANIELA ,  STREIDL THOMAS ,  HUNT DUVALL STACY

IPC: C12N9/04

Abstract: Una 3-hidroxibutirato deshidrogenasa (3-HBDH) mutante de Alcaligenes faecalis con un rendimiento mejorado en relación con la 3-HBDH natural, en la que la enzima mutante comprende una secuencia de aminoácidos que es al menos 80 % idéntica a la secuencia de aminoácidos de SEQ ID NO: 1 (3-HBDH de Alcaligenes faecalis; 3-HBDH natural) o la secuencia de aminoácidos de SEQ ID NO: 2 (secuencia central extendida de 3-HBDH natural) y en la que la enzima mutante tiene al menos tres sustituciones de aminoácidos en relación con la 3-HBDH natural, en la que el aminoácido en la posición correspondiente a - la posición 253 de SEQ ID NO: 1 se sustituye con Ile (253Ile), Ala (253Ala) o Cys (253Cys), - la posición 233 de SEQ ID NO: 1 se sustituye con Ser (233Ser), Ile (233Ile), Ala (233Ala), Pro (233Pro), Arg (233Arg), Thr (233Thr), Lys (233Lys) o Cys (233Cys), y - la posición 234 de SEQ ID NO: 1 se sustituye con Thr (234Thr).

公开(公告)号：CA2649135C

公开(公告)日：2013-11-19

申请号：CA2649135

申请日：2007-04-11

Applicant: HOFFMANN LA ROCHE

Inventor： BOENITZ-DULAT MARA ,  BECK DANIELA ,  KRATZSCH PETER ,  SCHMUCK RAINER ,  VON DER ELTZ HERBERT

IPC: C12N9/04

Abstract: The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticuss-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.

公开(公告)号：BRPI0710131A2

公开(公告)日：2011-08-02

申请号：BRPI0710131

申请日：2007-04-11

Applicant: HOFFMANN LA ROCHE

Inventor： BOENITZ-DULAT MARA ,  BECK DANIELA ,  KRATZSCH PETER ,  SCHMUCK RAINER ,  VON DER ELTZ HERBERT

IPC: C12N9/04

Abstract: The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.

公开(公告)号：CA2427029C

公开(公告)日：2010-02-02

申请号：CA2427029

申请日：2001-10-20

Applicant: HOFFMANN LA ROCHE

Inventor： KNAPPE WOLFGANG-REINHOLD ,  SCHMUCK RAINER ,  KRATZSCH PETER ,  SHAO ZHIXIN ,  BECK DANIELA ,  THYM DETLEF

IPC: C12N15/53 ,  G01N33/66 ,  C12M1/40 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04 ,  C12N11/02 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/32 ,  C12Q1/54

Abstract: The present invention relates to improved variants of soluble pyrroloquinoli ne quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specifici ty for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.

公开(公告)号：ES2624805T3

公开(公告)日：2017-07-17

申请号：ES07724148

申请日：2007-04-11

Applicant: HOFFMANN LA ROCHE

Inventor： BOENITZ-DULAT MARA ,  BECK DANIELA ,  KRATZSCH PETER ,  SCHMUCK RAINER ,  VON DER ELTZ HERBERT

IPC: C12N9/04

Abstract: Un mutante de glucosa deshidrogenasa soluble dependiente de PQQ (s-GDH; EC 1.1.5.2) con especificidad mejorada por la glucosa en comparación con la maltosa, que tiene una sustitución de treonina en la posición 348 por glicina, alanina o bien serina, en el que dicho mutante comprende adicionalmente a) al menos una mutación para mejorar la estabilidad del mutante, en el que dicha sustitución para mejorar la estabilidad se selecciona del grupo que consiste en D87R; N122K; S124K; S146G; V298L y L386F, b) al menos una mutación para mejorar la afinidad del mutante por la glucosa, y opcionalmente c) una o más mutaciones para mejorar aún más la especificidad del mutante por la glucosa en comparación con la maltosa, y en el que estas posiciones corresponden a las posiciones de aminoácidos conocidas de la secuencia natural de s-GDH de A. calcoaceticus (SEQ ID NO: 2).

公开(公告)号：CA2649135A1

公开(公告)日：2007-10-25

申请号：CA2649135

申请日：2007-04-11

Applicant: HOFFMANN LA ROCHE

Inventor： KRATZSCH PETER ,  BECK DANIELA ,  VON DER ELTZ HERBERT ,  BOENITZ-DULAT MARA ,  SCHMUCK RAINER

IPC: C12N9/04

Abstract: The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.