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    Compositions and methods for helper-free production of recombinant adeno-associated viruses
    11.
    发明授权
    Compositions and methods for helper-free production of recombinant adeno-associated viruses 有权
    无辅助生产重组腺相关病毒的组合物和方法

    公开(公告)号：US06485966B2

    公开(公告)日：2002-11-26

    申请号：US09826510

    申请日：2001-04-05

    Applicant: Guangping Gao ,  James M. Wilson

    Inventor： Guangping Gao ,  James M. Wilson

    IPC: C12N15861

    CPC classification number: G01R31/275 ,  C12N15/86 ,  C12N2710/10344 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2840/20 ,  G01R31/013 ,  G01R31/316

    Abstract: A method for producing recombinant adeno-associated virus in the absence of contaminating helper virus or wild-type virus involves culturing a mammalian host cell containing an rAd/AAV hybrid virus, an AAV rep sequence and an AAV cap sequence under the control of regulatory sequences directing expression thereof. The rAd/AAV hybrid virus contains a rAAV construct to be packaged into an AAV virion in an backbone containing the adenoviral sequences necessary to express E1a and E1b gene products and to permit replication of the hybrid virus. The method of the invention permits replication of the hybrid virus and production of rAAV virion in this host cell in the absence of a helper virus and obviates a subsequent purification step to purify rAAV from contaminating virus.

    Abstract translation: 在不存在污染性辅助病毒或野生型病毒的情况下用于产生重组腺相关病毒的方法包括在调节序列的控制下培养含有rAd / AAV杂合病毒，AAV rep序列和AAV帽序列的哺乳动物宿主细胞 指导其表达。 rAd / AAV杂合病毒含有一个rAAV构建体，其被包装在含有表达E1a和E1b基因产物所必需的腺病毒序列并允许复制病毒的骨架中的AAV病毒粒子中。 本发明的方法允许在不存在辅助病毒的情况下复制该杂种病毒并在该宿主细胞中产生rAAV病毒粒子，并且消除随后的纯化步骤以从污染病毒纯化rAAV。

    Adenovirus gene therapy vehicle and cell line
    13.
    发明授权
    Adenovirus gene therapy vehicle and cell line 失效
    腺病毒基因治疗载体和细胞系

    公开(公告)号：US06281010B1

    公开(公告)日：2001-08-28

    申请号：US08549489

    申请日：1995-10-27

    Applicant: Guang-Ping Gao ,  James M. Wilson

    Inventor： Guang-Ping Gao ,  James M. Wilson

    IPC: C12N1500

    CPC classification number: C12N15/86 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2750/14143 ,  Y10S977/802 ,  Y10S977/907

    Abstract: A novel adenovirus E1/E4 expressing packaging cell line is provided, which permits the generation of recombinant adenoviruses deleted in both gene regions. The E1/E4 deleted recombinant adenovirus is capable of expressing a selected transgene product in cells in vivo or in vitro. This recombinant virus is useful in the treatment of genetic disorders.

    Abstract translation: 提供了表达新型腺病毒E1 / E4的包装细胞系，其能够在两个基因区域中产生缺失的重组腺病毒。 E1 / E4缺失的重组腺病毒能够在体内或体外在细胞中表达所选择的转基因产物。 这种重组病毒可用于治疗遗传疾病。

    Multiple wobble correction optical elements to reduce height of raster output scanning (ROS) system
    14.
    发明授权
    Multiple wobble correction optical elements to reduce height of raster output scanning (ROS) system 有权
    多个摆动校正光学元件，以减少光栅输出扫描（ROS）系统的高度

    公开(公告)号：US06252695B1

    公开(公告)日：2001-06-26

    申请号：US09467756

    申请日：1999-12-20

    Applicant: James M. Wilson

    Inventor： James M. Wilson

    IPC: G02B2608

    CPC classification number: G02B26/126

    Abstract: Two shorter focal length wobble correction optical elements reduce the height of a raster output scanning (ROS) system. The wobble correction optical elements can be two lenses or two mirrors.

    Abstract translation: 两个较短的焦距摆动校正光学元件降低了光栅输出扫描（ROS）系统的高度。 摆动校正光学元件可以是两个镜头或两个镜子。

    Method for tolerizing a mammalian patient to administration of gene therapy virus vectors
    16.
    发明授权
    Method for tolerizing a mammalian patient to administration of gene therapy virus vectors 失效
    用于耐受哺乳动物患者施用基因治疗病毒载体的方法

    公开(公告)号：US06211160B1

    公开(公告)日：2001-04-03

    申请号：US08889930

    申请日：1997-07-10

    Applicant: James M. Wilson ,  Youhai Chen

    Inventor： James M. Wilson ,  Youhai Chen

    IPC: A61K4800

    CPC classification number: A61K48/00

    Abstract: A method for tolerizing a mammalian subject to administration of a live virus carrying a gene for delivery to a cell of the subject is disclosed. The method entails administering to the subject a suitable amount of an inactivated virus prior to administration of the live virus. The prior administration of the inactivated virus suppresses anti-virus cytotoxic T cells, permitting longer transgene persistence once the live virus is administered, and permitting effective readministration of live virus.

    Abstract translation: 公开了一种用于耐受哺乳动物受试者施用携带用于递送至受试者细胞的基因的活病毒的方法。 该方法需要在施用活病毒之前向受试者施用适量的灭活病毒。 先前施用灭活病毒抑制抗病毒细胞毒性T细胞，一旦施用活病毒就允许更长的转基因持久性，并且允许有效重新施用活病毒。

    Thin film organic light emitting diode with edge emitter waveguide and electron injection layer
    17.
    发明授权
    Thin film organic light emitting diode with edge emitter waveguide and electron injection layer 失效
    薄膜有机发光二极管与边缘发射波导和电子注入层

    公开(公告)号：US5994835A

    公开(公告)日：1999-11-30

    申请号：US782134

    申请日：1997-01-13

    Applicant: James M. Wilson ,  David K. Fork

    Inventor： James M. Wilson ,  David K. Fork

    IPC: G02B6/42 ,  H01L51/50 ,  H01L51/52 ,  H01J1/02

    CPC classification number: G02B6/42 ,  H01L51/5262 ,  H01L51/5092 ,  Y10S428/917

    Abstract: A thin film organic light emitting diode with edge emitter waveguide comprises, in sequence, a substrate, a waveguide, an anode, a hole transport layer, an electroluminescent layer, an electron injection layer and a cathode. Voltage applied between the anode and cathode causes the electroluminescent layer to emit light through the hole transport layer and the anode into the waveguide where the light is internally reflected within the waveguide and propagates through the length of the waveguide to be emitted through the edge of the waveguide. The electron injection layer also functions as a cladding layer to increase the efficiency of the waveguide. The non-emission edge of the waveguide may be sloped with a conductor layer to the anode and a conductor layer to the cathode forming a reflective surface to the non-emission edge.

    Abstract translation: 具有边缘发射波导的薄膜有机发光二极管依次包括衬底，波导，阳极，空穴传输层，电致发光层，电子注入层和阴极。 施加在阳极和阴极之间的电压使得电致发光层通过空穴传输层和阳极发射到波导中，其中光在波导内部被反射并且在波导的长度内传播以通过波导的边缘发射 波导。 电子注入层也用作包覆层以提高波导的效率。 波导的非发射边缘可以与导体层倾斜到阳极，并且导体层向阴极形成到非发射边缘的反射表面。

    Method for improved production of recombinant adeno-associated viruses for gene therapy
    19.
    发明授权
    Method for improved production of recombinant adeno-associated viruses for gene therapy 失效
    通过重组腺相关病毒改善转导的方法

    公开(公告)号：US5756283A

    公开(公告)日：1998-05-26

    申请号：US462014

    申请日：1995-06-05

    Applicant: James M. Wilson ,  Krishna J. Fisher ,  Guang-Ping Gao

    Inventor： James M. Wilson ,  Krishna J. Fisher ,  Guang-Ping Gao

    IPC: A61K48/00 ,  C12N15/864 ,  C12Q1/70 ,  C12N7/04 ,  C12N13/00 ,  C12N15/00

    CPC classification number: C12N15/86 ,  A61K48/00 ,  C12N2750/14143

    Abstract: A method for enhancing the efficiency of transduction of a recombinant AAV into a target cell is provided. The target cell is infected with a recombinant adeno-associated virus comprising a selected transgene under the control of regulatory sequences. The infected cell is contacted with an agent which facilitates the conversion of single stranded recombinant virus to its double stranded form. When this conversion occurs in the target cell, enhanced transduction of the recombinant virus into said target cell results. The agent can be a helper virus providing a gene which facilitates the conversion, or an agent to which the infected cell is exposed, which facilitates the conversion. In a similar manner, a novel recombinant AAV is provided which contains the facilitating gene and the transgene. The methods may be performed ex vivo or in vivo.

    Abstract translation: 提供了一种用于提高重组AAV转导入靶细胞的效率的方法。 靶细胞用包含选择的转基因的重组腺相关病毒感染，在调控序列的控制下。 将感染的细胞与促进单链重组病毒转化为其双链形式的试剂接触。 当转化发生在靶细胞中时，将重组病毒增强转导到所述靶细胞中。 该试剂可以是提供有助于转化的基因的辅助病毒，或感染细胞暴露于其中的试剂，其有助于转化。 以类似的方式，提供了含有促进基因和转基因的新型重组AAV。 该方法可以离体或体内进行。

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