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公开(公告)号：US11933972B2
公开(公告)日：2024-03-19
申请号：US17423924
申请日：2019-12-19
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Andreas Lotter , Christian Schulz , Christian Schumann
CPC classification number: G02B27/0025 , G02B15/143 , G02B15/20 , G02B21/025 , G02B21/36
Abstract: An optical system for a microscope for imaging an object includes: a telescope system having an optical correction unit, which is adjustable in order to correct a spherical imaging aberration, and having a zoom optical unit, which is adjustable in order to adapt a magnification of the telescope system to a ratio of two refractive indices, one of which is assigned to an object side and an other of which is assigned to an image side, within a predetermined magnification range. The telescope system is telecentric over an entire magnification range both with respect to the object side and with respect to the image side by the zoom optical unit contained in the telescope system.
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公开(公告)号：US11635608B2
公开(公告)日：2023-04-25
申请号：US17285481
申请日：2019-10-11
Applicant: Leica Microsystems CMS GmbH
Inventor： Alexander Weiss , Christian Schumann , Ronja Capellmann
Abstract: A method is useable for determining a refractive index of an optical medium in a microscope, which has an objective facing toward a sample chamber. The optical medium is one of two optical media, which border two opposing surfaces of a cover slip or object carrier in the sample chamber and form two partially reflective interfaces, which are arranged at different distances from the objective. The method includes: deflecting a measurement light beam by the objective with oblique incidence on the cover slip or object carrier; generating two reflection light beams spatially separated from one another by the measurement light beam being partially reflected at each of the interfaces; receiving the two reflection light beams by the objective and conducting them onto a position-sensitive detector; registering intensities by the position-sensitive detector; and determining the refractive index of the optical medium based on the registered intensities.
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13.发明授权公开(公告)号：US11536939B2
公开(公告)日：2022-12-27
申请号：US17285104
申请日：2019-10-18
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian Schumann , Tobias Bauer
Abstract: A method is useable for digitally correcting an optical image of a sample by a microscope that has a cover slip covering the sample. The method includes: determining, by the microscope, an index of refraction of an optical medium bordering the cover slip, a tilt of the cover slip, and/or a thickness of the cover slip; ascertaining an imaging error to be corrected in the form of a pupil function based on the index of refraction of the optical medium, the tilt of the cover slip, and/or the thickness of the cover slip; carrying out imaging of the sample by the microscope; and digitally correcting image data captured by the imaging of the sample based on the pupil function.
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公开(公告)号：US10429630B2
公开(公告)日：2019-10-01
申请号：US15771341
申请日：2016-10-28
Applicant: Leica Microsystems CMS GmbH
Inventor： Benjamin Deissler , Christian Schumann
Abstract: A fluorescence microscope has an incident fluorescence illumination unit generating an incident fluorescence illumination beam path a multi-band fluorescence filter system encompassing a multi-band beam splitter for deflecting the incident fluorescence illumination beam path into an objective of the microscope and onto a specimen. The filter system has a multi-band blocking filter that at least partly transmits a fluorescence emission beam path proceeding from the specimen; and having a digital camera for generating a fluorescence image of the specimen. The microscope has a bright-field transmitted illumination unit for generating a bright-field transmitted illumination beam path for transmitted illumination of the specimen. The filter system is arranged fixedly so that upon acquisition of a bright-field image of the specimen, the system remains in a bright-field transmitted light beam path proceeding from the specimen. The digital camera has a white balance function that is executed before the bright-field image is generated.
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公开(公告)号：US20240094518A1
公开(公告)日：2024-03-21
申请号：US17947257
申请日：2022-09-19
Applicant: Leica Microsystems CMS GmbH
Inventor： Benjamin Deissler , Christian Schulz , Christian Schumann
CPC classification number: G02B21/16 , G01N21/6458 , G02B21/08
Abstract: A microscope for fluorescence light imaging includes an illumination device configured to direct excitation light along an excitation beam path into a sample, and a detection device configured to detect fluorescence light emanating from the sample along a detection beam path which is located in a same hemisphere relative to the sample as the excitation beam path, wherein the illumination device includes a light guiding system configured to guide the excitation light such that, in phase space defined by position and angle of a light beam, a support of the excitation beam path is disjunct from a support of the detection beam path, and wherein the detection device includes at least one emission filter configured to allow the fluorescence light propagating along the detection beam path to be detected for fluorescence light imaging while discarding light that is spectrally different from the fluorescence light.
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Patent Agency Ranking
16.发明授权公开(公告)号：US11500189B2
公开(公告)日：2022-11-15
申请号：US17599646
申请日：2020-03-25
Applicant: Leica Microsystems CMS GmbH
Inventor： Alexander Weiss , Christian Schumann , Ronja Capellmann
Abstract: A light sheet microscope includes a sample chamber in which a cover slip or slide is arrangeable, which has a surface that defines a partially reflective interface and which has a further surface that defines a further partially reflective interface. The two interfaces are arranged at different distances from an objective. The light sheet microscope further includes an optical system having the objective facing toward the cover slip or slide, an illumination apparatus, which is designed to generate a light sheet, a sensor, and a processor. The two interfaces are formed in that two optical media are applicable in the sample chamber. The light sheet microscope forms a measuring device for acquiring a measured variable. The sensor is designed to acquire the intensities and/or the incidence locations of the two reflection light beams.
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公开(公告)号：US20210349298A1
公开(公告)日：2021-11-11
申请号：US17285103
申请日：2019-10-18
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Alexander Weiss , Christian Schumann , Ronja Capellmann
Abstract: A method is useable for determining a thickness of a cover slip or object carrier in a microscope, which has an objective facing toward a sample chamber. Two optical media border two opposing surfaces of the cover slip or object carrier and form two partially reflective interfaces, which are arranged at different distances from the objective. The method includes: deflecting a measurement light beam by the objective with oblique incidence on the cover slip or object carrier; generating two reflection light beams spatially separated from one another by the measurement light beam being partially reflected on each of the two interfaces; receiving the two reflection light beams by the objective and conducting them onto a position-sensitive detector; registering the incidence locations on the position-sensitive detector; and determining the thickness of the cover slip or object carrier based on the registered incidence locations.
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公开(公告)号：US11086117B2
公开(公告)日：2021-08-10
申请号：US16098183
申请日：2017-05-03
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian Schumann
Abstract: An apparatus for light-sheet-like light illumination of a sample includes a light source configured to generate an illumination beam. A focusing system is configured to focus the illumination beam to form a light-sheet-like illumination light distribution, with which a focal plane of the sample can be illuminated. An imaging optical unit is configured to image the light-sheet-like illumination light distribution into the focal plane. A polarization element, arranged in a position conjugated to the focal plane between the focusing system and the imaging optical unit, is configured to split the illumination beam into two differently polarized sub-beams, which propagate into the imaging optical unit in different propagation directions, whereby the light-sheet-like illumination light distribution can be imaged by the imaging optical unit in the form of two differently polarized light-sheets, which from a same side of the focal plane are superimposed on each other in the focal plane.
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公开(公告)号：US11067781B2
公开(公告)日：2021-07-20
申请号：US16098472
申请日：2017-05-02
Applicant: Leica Microsystems CMS GmbH
Inventor： Florian Fahrbach , Frank Sieckmann , Christian Schumann , Oliver Schlicker
Abstract: A microscope includes illumination optics for fluorescence excitation of point light sources of a sample, detection optics and a camera having a sensor. A density of the point light sources is kept low so as to minimize a crossover of point light sources that are behind or close to one another in each image captured by the camera. A means for subdividing a detection aperture into individual sub-apertures is provided in a beam path of the detection optics such that images generated by the individual sub-apertures on the sensor of the camera depict an object volume from different spatial directions.
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公开(公告)号：US20190243118A1
公开(公告)日：2019-08-08
申请号：US16340140
申请日：2017-10-06
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian Schumann
CPC classification number: G02B21/361 , G01N21/6458 , G02B21/0032 , G02B21/0076 , G02B21/367
Abstract: An oblique plane microscope includes a detection optical unit having an image sensor which has a sensor surface formed from sensor lines arranged in parallel, and a transport optical unit having an objective arranged for specimen illumination by a light sheet tilted relative to an optical axis of the transport optical unit and for imaging a specimen plane illuminated with the light sheet onto the sensor surface. An optical axis of the detection optical unit is tilted relative to the optical axis of the transport optical unit. The sensor lines each extend in an orthogonal direction with respect to the optical axis of the transport optical unit. The detection optical unit has an anamorphic magnification system. A magnification of an anamorphic magnification system of the detection optical unit, in a direction lying orthogonal to the sensor lines, is less than in a direction lying parallel to the sensor lines.
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