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    Time-Warped Background Signal for Sequencing-by-Synthesis Operations
    11.
    发明申请
    Time-Warped Background Signal for Sequencing-by-Synthesis Operations 审中-公开

    公开(公告)号：US20170300616A1

    公开(公告)日：2017-10-19

    申请号：US15454006

    申请日：2017-03-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell

    IPC: G06F19/24

    Abstract: Methods for analyzing signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the method comprises formulating a function that models the output signal of a representative empty well of a reactor array. A time transformation is applied to the empty well function to obtain a time-warped empty well function. The time-warped empty well function is fitted to an output signal from the loaded well representative of a flow that results in a non-incorporation event in the loaded well. The fitted time-warped empty well function can then be used to analyze output signals from the loaded well for other flows.

    METHODS, SYSTEMS, COMPUTER READABLE MEDIA, AND KITS FOR SAMPLE IDENTIFICATION
    12.
    发明申请
    METHODS, SYSTEMS, COMPUTER READABLE MEDIA, AND KITS FOR SAMPLE IDENTIFICATION 有权

    公开(公告)号：US20250137049A1

    公开(公告)日：2025-05-01

    申请号：US18949259

    申请日：2024-11-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell

    IPC: C12Q1/6874 ,  C12Q1/6869 ,  C40B20/04 ,  C40B50/16 ,  C40B70/00 ,  G16B30/00 ,  G16C20/64 ,  G16C99/00

    Abstract: A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.

    Combinatorial barcode sequences, and related systems and methods
    13.
    发明授权
    Combinatorial barcode sequences, and related systems and methods 有权

    公开(公告)号：US12264363B2

    公开(公告)日：2025-04-01

    申请号：US17546151

    申请日：2021-12-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell

    IPC: C12Q1/6874 ,  B01J19/00 ,  C12N15/10 ,  C40B20/04 ,  C40B70/00

    Abstract: A system for nucleic acid sequencing is provided. The system comprises a sequencing device configured to expose a tagged polynucleotide comprising a combinatorial barcode sequence and a sample polynucleotide to sequential nucleotide flows, each flow comprising one species of nucleotide and the sequential flows being in a predetermined order based on the species of nucleotide such that exposing of the tagged polynucleotide to the sequential nucleotide flows causes incorporations of nucleotides from the sequential nucleotide flows into the tagged polynucleotide over the barcode sequence The sequencing device is configured to detect a series of signals over the barcode sequence resulting from the incorporations, wherein the predetermined order of nucleotide flows comprises a repetition of a flow order motif that is based on a sequence motif. The system comprises a computing device configured to resolve the detected series of signals to determine the combinatorial barcode sequence.

    Methods, systems, and computer readable media for repeat sequencing
    14.
    发明授权
    Methods, systems, and computer readable media for repeat sequencing 有权

    公开(公告)号：US12077818B2

    公开(公告)日：2024-09-03

    申请号：US18297049

    申请日：2023-04-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian Koller ,  Nils Homer

    IPC: C12Q1/6869 ,  G16B30/00

    CPC classification number: C12Q1/6869 ,  G16B30/00 ,  C12Q2535/122 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2535/119 ,  C12Q2537/165

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR REPEAT SEQUENCING
    15.
    发明公开
    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR REPEAT SEQUENCING 审中-公开

    公开(公告)号：US20230348966A1

    公开(公告)日：2023-11-02

    申请号：US18297049

    申请日：2023-04-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian Koller ,  Nils Homer

    IPC: C12Q1/6869 ,  G16B30/00

    CPC classification number: C12Q1/6869 ,  G16B30/00 ,  C12Q2535/122

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

    Methods, systems, and computer readable media for repeat sequencing
    17.
    发明授权
    Methods, systems, and computer readable media for repeat sequencing 有权

    公开(公告)号：US10329608B2

    公开(公告)日：2019-06-25

    申请号：US14048843

    申请日：2013-10-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian Koller ,  Nils Homer

    IPC: C12Q1/6869

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

    Methods, Systems, and Computer Readable Media for Repeat Sequencing
    19.
    发明申请
    Methods, Systems, and Computer Readable Media for Repeat Sequencing 审中-公开
    方法，系统和计算机可读介质重复测序

    公开(公告)号：US20140113281A1

    公开(公告)日：2014-04-24

    申请号：US14048843

    申请日：2013-10-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian KOLLER ,  Nils HOMER

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q2535/122 ,  C12Q2525/179 ,  C12Q2535/119 ,  C12Q2537/165

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

    Abstract translation: 用于核酸模板测序的方法包括：（a）进行第一测序过程，包括根据核苷酸和/或试剂的第一预定顺序将核苷酸和/或试剂流向核酸模板以获得第一测序结果; （b）在第一测序过程之后，进行第二测序过程，包括根据核苷酸和/或试剂的第二预定顺序将核苷酸和/或试剂流向核酸模板以获得第二测序结果，第二预定顺序 核苷酸和/或试剂不同于核苷酸和/或试剂的第一预定顺序，并且设计用于重复测序的核苷酸和/或试剂的第一和第二预定顺序中的至少一个; 和（c）使用第一测序结果和第二测序结果两者来确定对应于核酸模板的至少一部分的碱基序列。

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