Abstract:
Abstract Devices and methods for sample preparation via pressure cycling technology are disclosed. The device for sample preparation comprises a tube having an inner surface, a top, and a bottom, the tube configured to contain a sample at the bottom and to be received into a pressure chamber for sample preparation, a cap detachably connected to the top of the tube, and a tapered elongate member extending from the cap into the tube, the tapered elongate member configured to contact the inner surface of the tube and the sample in the bottom of the tube, wherein the tube is deformable such that in operation under pressure the tube is deformed against the tapered elongate member to promote disruption of the sample.
Abstract:
A high pressure valve includes a lever and a variable force generator for facilitating control of opening and closing the valve at high pressures. The high pressure valve includes a housing having a chamber providing fluid communication between a first port and a second port, a pin movable within the chamber between an open position and a closed position. A first end of a lever is coupled to the pin and a second end of the lever is coupled to a variable force generator. The lever pivots about a pivot point. A controller coupled to the variable force generator is configured to adjust a force applied to the second end of the lever by the variable force generator to control the movement of the pin between the open position and the closed position.
Abstract:
The present invention is related to systems and methods for chemical and biological analysis and, in particular, to systems, apparatus, and methods of sample conditioning and analysis.
Abstract:
Extraction methods that allow a molecular complex (e.g., an organelle) to be extracted from a sample by employing pressure cycling are described.
Abstract:
Devices and methods for sample preparation via pressure cycling technology are disclosed. The device for sample preparation comprises a tube having an inner surface, a top, and a bottom, the tube configured to contain a sample at the bottom and to be received into a pressure chamber for sample preparation, a cap detachably connected to the top of the tube, and a tapered elongate member extending from the cap into the tube, the tapered elongate member configured to contact the inner surface of the tube and the sample in the bottom of the tube, wherein the tube is deformable such that in operation under pressure the tube is deformed against the tapered elongate member to promote disruption of the sample.
Abstract:
Una válvula de alta presión (100, 200, 300) que comprende: una carcasa (40, 50, 30) que tiene una cámara (41), un primer puerto (42) configurado para permitir que el fluido fluya a través del mismo, un segundo puerto (54) configurado para permitir que el fluido fluya a través del mismo, la cámara que proporciona comunicación de fluidos entre el primer puerto y el segundo puerto; un pasador (20) que puede moverse dentro de la cámara entre una posición abierta en la que el primer puerto y el segundo puerto están en comunicación de fluidos y una posición cerrada en la que el primer puerto y el segundo puerto no están en comunicación de fluidos; un anillo de respaldo (14) que guía el pasador, el anillo de respaldo que tiene una geometría cóncava en su cara inferior; un sello (16) que tiene un diámetro interior a través del cual pasa el pasador y una superficie de extremo con una geometría esférica configurada para coincidir con la superficie cóncava del anillo de respaldo, en donde la alta presión en la cámara hace que el sello se deforme hacia adentro contra el pasador y se ensanche una pared de diámetro exterior del anillo de respaldo hacia fuera; una palanca (2) que tiene un primer extremo (110) acoplado al pasador y un segundo extremo (112), la palanca que tiene un punto de pivote (114) que está asegurado de manera giratoria a la carcasa; un generador de fuerza variable conectado al segundo extremo de la palanca y configurado para mover el pasador entre la posición abierta y la posición cerrada; y un controlador (400) acoplado al generador de fuerza variable, el controlador que se configura para ajustar una fuerza aplicada al segundo extremo de la palanca por el generador de fuerza variable para controlar el movimiento del pasador entre la posición abierta y la posición cerrada, en donde la válvula de alta presión se configura para operar a una presión de al menos 137,90 MPa (20.000 psi) medida en la cámara.
Abstract:
A valve includes a valve seat body received by a sleeve with an interference fit between the valve seat body and the sleeve. An upper end of the sleeve extends beyond an upper end of the valve seat body. A valve assembly including the valve is also provided.