Abstract:
Extraction methods that allow a molecular complex (e.g., an organelle) to be extracted from a sample by employing pressure cycling are described.
Abstract:
Devices and methods for sample preparation via pressure cycling technology are disclosed. The device for sample preparation comprises a tube having an inner surface, a top, and a bottom, the tube configured to contain a sample at the bottom and to be received into a pressure chamber for sample preparation, a cap detachably connected to the top of the tube, and a tapered elongate member extending from the cap into the tube, the tapered elongate member configured to contact the inner surface of the tube and the sample in the bottom of the tube, wherein the tube is deformable such that in operation under pressure the tube is deformed against the tapered elongate member to promote disruption of the sample.
Abstract:
Extraction methods that allow a molecular complex (e.g., an organelle) to be extracted from a sample by employing pressure cycling are described.
Abstract:
Sample processing devices having collapsible, flexible bodies are disclosed. The flexible microtube sampling devices are utilizable in pressure mediated, pressure cycling procedures. Externally applied pressure on the flexible microtube sample processing devices allow buckling thereof and transfer of the applied pressure to the sample contained therein.
Abstract:
The systems and techniques of the present invention can also synergistically utilize mechanical disruption processes with the use of high hydrostatic pressure extraction, such as pressure cycling extraction techniques to achieve high yield of difficult to extract sample constituents without generating high shear stress or high temperatures.
Abstract:
A high pressure valve includes a lever and a variable force generator for facilitating control of opening and closing the valve at high pressures. The high pressure valve includes a housing having a chamber providing fluid communication between a first port and a second port, a pin movable within the chamber between an open position and a closed position. A first end of a lever is coupled to the pin and a second end of the lever is coupled to a variable force generator. The lever pivots about a pivot point. A controller coupled to the variable force generator is configured to adjust a force applied to the second end of the lever by the variable force generator to control the movement of the pin between the open position and the closed position.
Abstract:
A valve includes a valve seat body received by a sleeve with an interference fit between the valve seat body and the sleeve. An upper end of the sleeve extends beyond an upper end of the valve seat body. A valve assembly including the valve is also provided.
Abstract:
The present invention is related to systems and methods for chemical and biological analysis and, in particular, to systems, apparatus, and methods of sample conditioning and analysis.
Abstract:
Devices and methods for sample preparation via pressure cycling technology are disclosed. The device for sample preparation comprises a tube having an inner surface, a top, and a bottom, the tube configured to contain a sample at the bottom and to be received into a pressure chamber for sample preparation, a cap detachably connected to the top of the tube, and a tapered elongate member extending from the cap into the tube, the tapered elongate member configured to contact the inner surface of the tube and the sample in the bottom of the tube, wherein the tube is deformable such that in operation under pressure the tube is deformed against the tapered elongate member to promote disruption of the sample.