公开(公告)号：JPH01221668A

公开(公告)日：1989-09-05

申请号：JP4730088

申请日：1988-02-29

Applicant: SHIMADZU CORP

Inventor： YAMAMOTO HIDEKI

IPC: G01N33/557 ,  C12M1/34 ,  G01N21/27 ,  G01N33/543

Abstract: PURPOSE:To quantitatively determine antigens or antibodies with good sensitivity by projecting light of respectively different wavelengths during the course of an antigen-antibody reaction and determining the difference or change of the absorbancy ratio at the respective points of the time. CONSTITUTION:Insoluble carriers of fine grain sizes consisting of polystyrene, polymethyl styrene, etc., water, and a fixing medium consisting of a dispersion medium such as salt water are mixed with the antibodies or antigens to be tested. The light of the different wavelength lambda1, lambda2 are projected to this mixture at least at >=2 points of the time during the course of the reaction of the antigen-antibody reaction generated at this time and the absorbancies A(lambda1), A(lambda2) are determined. The ratio A(lambda1)/A(lambda2) of these absorbancies is determined and the antigens or antibodies are determined in accordance with the difference or change of the absorbancy ratio at the above-mentioned respective points of the time.

公开(公告)号：JPS60253978A

公开(公告)日：1985-12-14

申请号：JP11261884

申请日：1984-05-31

Applicant: SHIMADZU CORP

Inventor： YAMAMOTO HIDEKI

IPC: G01N21/75 ,  G01N21/27 ,  G01N35/02

Abstract: PURPOSE:To make drift compensation of a photoelectric detector without bisecting irradiating light by storing the outputs from the photoelectric detector when no photometric cells exist in an optical path and when the cells exist therein via a moving mechanism and calculating absorbancy therefrom. CONSTITUTION:A turntable 2 to which plural photometric cells 1 are attached stops in the position where irradiating light 3 passes between the two photometric cells 1 and 1'. The output from a photomultiplier 4 which receives the irradiating light enters a control circuit 8 for voltage of a negative high voltage power source via an amplifier 5, a switch 6 and a holding circuit 7. The power source voltage is controlled by said circuit to maintain the specified output of the amplifier 5. The output V0 of the amplifier 5 is stored via an AD converter 9 into a computer 10. The switch 6 is opened by the command from the computer 10 to rotate the turntable 2 so that the cell 1 is moved into the optical path. The output of the amplifier 5 is stored via the converer 9 into the computer 10. Concn. is determined by using a calibration curve from -logVt/V0.

公开(公告)号：JPH01196573A

公开(公告)日：1989-08-08

申请号：JP2084788

申请日：1988-01-30

Applicant: SHIMADZU CORP

Inventor： YAMAMOTO HIDEKI

IPC: G01N35/10

Abstract: PURPOSE:To prevent the effect on the following analysis due to the exhaustion of a reagent, by measuring the amount of the reagent in a specimen container and distributing a diluent to a reaction container short in the amount of the reagent. CONSTITUTION:A specimen is distributed to each of reaction cuvets 10 from a specimen cup at a specimen distributing position and a cuvet rotor 38 is rotated to feed each of the cuvets 10 to a reagent distributing position 9. The distribution of a reagent 41 is performed by inserting a reagent distribution nozzle 2 and a liquid level sensor 3 in a reagent container 7 to confirm that both of them area immersed in the reagent 41, by a computer 32 and subsequently sucking and collecting the reagent 41 by a reagent distribution pump 20 to move the reagent distribution nozzle to the position above each of the cuvets 10. When a liquid level is not detected by the liquid level sensor 3, a washing solution is distributed to the cuvet 10 form a washing solution sump 17 on the basis of the signal from the computer 32. By this method, it can be prevented that a precipitate is bonded to the inner wall of the cuvet 10 by the evaporation of moisture from the specimen at the time of the exhaustion of the reagent.

公开(公告)号：JPS62179639A

公开(公告)日：1987-08-06

申请号：JP2106086

申请日：1986-01-31

Applicant: SHIMADZU CORP

Inventor： MATSUMOTO JUNICHI ,  YAMAMOTO HIDEKI

IPC: G01N21/75 ,  G01N35/00

Abstract: PURPOSE:To remove errors due to turbid components without use of a number of blank channels or a reference liquid for turbidity, by a method wherein a sample containing suspension material is divided by every item, is mixed with a reaction liquid to measure absorbance and correction of a blank absorbance determined by a specified method is applied. CONSTITUTION:A sample containing suspension material is divided by every item and mixed with a reaction agent for item-wise measurement to make a measuring liquid. Absorbance with a specified wavelength of each measuring liquid is measured. On the other hand, the sample is mixed with a blank reaction reagent excluding reaction components from a reaction reagent to prepare a sample blank liquid. The absorbance of the blank liquid is measured at least about two kinds of wavelengths to obtain a wavelength-absorbance regression function. A blank absorbance at the measuring wavelength is calculated about respective items from the regression function to correct absorbance of each measuring liquid.

公开(公告)号：JPS6180047A

公开(公告)日：1986-04-23

申请号：JP20382784

申请日：1984-09-28

Applicant: Shimadzu Corp

Inventor： YAMAMOTO HIDEKI

IPC: G01N33/536 ,  G01N33/557

CPC classification number: G01N33/557

Abstract: PURPOSE:To enhance a treatment speed, in the measurement of immune reaction according to a rate method using nephelometry, by estimating the max. value of a reaction speed without performing continuous photometry. CONSTITUTION:The curve showing the relation between the concn. of the antigen-antibody composite substance and reaction time in antigen-antibody reaction resembles to the curve at the central part of a cubic curve as shown by the drawing. A reaction reagent is successively added to a plurality of specimens to react an antigen and an antibody while the intensity of scattered light is measured in the addition order of the reagent and, when measurement was finished once, the intensity of scattered light is repeatedly measured from the first specimen in the same way and this measurement is performed at least three times with respect to each specimen to calculate the cubic expression, that is, constants (a), (b), (c) of formula I (wherein I is the intensity of scattered light and T is reaction elapse time) with respect to each specimen. These constants are substituted for formula II to calculate the max. reaction speed in the antigen/antibody between the specimen and the reagent.

Abstract translation: 目的：为了提高治疗速度，在使用比浊法测定免疫反应的速率方法时，通过估算最大值。 没有进行连续光度测定的反应速度的值。 构成：显示浓缩物之间关系的曲线。 的抗原 - 抗体复合物质和抗原 - 抗体反应中的反应时间类似于如图所示的三次曲线的中心部分的曲线。 将反应试剂连续添加到多个试样中以使抗原和抗体反应，同时按照试剂的加成顺序测量散射光的强度，并且当测量完成一次时，从...重复测量散射光的强度 以相同的方式测量第一个样品，并且对每个样品进行至少三次测量，以计算立方体表达式，即式I的常数（a），（b），（c）（其中I为 相对于每个样品，散射光的强度和T是反应时间）。 这些常数代入式Ⅱ计算最大值。 样品与试剂之间抗原/抗体的反应速度。

公开(公告)号：JPS6011167A

公开(公告)日：1985-01-21

申请号：JP11983683

申请日：1983-06-30

Applicant: SHIMADZU CORP

Inventor： YAMAMOTO HIDEKI

IPC: G01N35/02 ,  G01N35/04

Abstract: PURPOSE:To obtain an automatic chemical analysis device which maintains the initial processing power for analysis without decrease even if analysis of items with different reaction times is performed by providing an auxiliary line for reaction by-passing the main line for reaction to said line. CONSTITUTION:A main line 2 for reaction of an automatic chemical analysis device 1 is provided with a means 5 for supplying reaction tubes which supplies successively the reaction tubes of the number corresponding to the number of the analyzing items to be assigned preliminarily for each specimen, a means 6 for dispensing the specimen, a means 7 for dispensing a reagent and a means 8 for measurement. The means 7 consists of the 1st dispensing means 9, the 2nd dispensing means 10 and the 3rd dispensing means 11. The analysis for each analyzing item is automatically accomplished with each of the reaction tubes by the command from a control part 4. The device 1 is provided with auxiliary lines 3, 3', 3'' for reaction, by which the reaction tubes having longer reaction time can be by-passed from the line 2 and stagnated. The measurement in the optimum reaction time is thus made possible without decreasing processing capacity for each analysis even if the multiple items are analyzed simultaneously with different reaction times.

公开(公告)号：JPS59202065A

公开(公告)日：1984-11-15

申请号：JP7703583

申请日：1983-04-30

Applicant: Shimadzu Corp

Inventor： YAMAMOTO HIDEKI

IPC: G01N35/10 ,  G01N35/06

CPC classification number: G01N35/10

Abstract: PURPOSE:To attain to enhance a specimen distributing degree and to also accurately perform the analysis of a high concn. specimen, by mixing and injecting both liquids from the non-diluted specimen container and the dilution liquid tank of a specimen table in a diluted specimen container while injecting the liquids of both containers or the liquid of one container into the reaction tube of a reaction table. CONSTITUTION:The movement of a nozzle 4, the suckings and emissions of a dilution liquid pump 7 and a non-diluted liquid pump 8 and the change-over of an emitting and flowline change-over valve 16 are controlled by a central control part 20 and the liquid mixture of the non-diluted specimen of each non-diluted specimen container 10 of an analytical turntable 2 and the diluted specimen from the diluted liquid tank 6 is distributed in diluted specimen containers 11. In addition, the liquid mixture from the tubes 11 or the non-diluted specimen from each tube 10 is similarily distributed in each reaction tube 17 in which a reagent is distributed of a reaction tube turntable 3 to perform absorbance analysis. By this constitution, specimen distributing accuracy is enhanced and an apparent concn. due to a prozone phenomenon is corrected by the comparison with concn. accompanying the analysis of the reagent in the non-diluted specimen even if a reagent concn. is high and accurate analysis can be performed.

Abstract translation: 目的：提高样本分布度，并准确执行高浓度分析。 通过将两个容器的液体或一个容器的液体注入到反应台的反应管中，将来自未稀释的样品容器和样品台的稀释液体罐的两种液体混合并注入到稀释的样品容器中 。 构成：喷嘴4的移动，稀释液体泵7和非稀释液体泵8的吸入和排放以及排放和流动切换阀16的转换由中央控制部分20控制 将分析转台2的每个未稀释的样本容器10的未稀释样品和来自稀释液体罐6的稀释样品的液体混合物分布在稀释的样品容器11中。此外，来自管的液体混合物 11或来自每个管10的未稀释的样品相似地分布在其中分布有反应管转盘3的试剂的每个反应管17中以进行吸光度分析。 通过这种结构，提高了样本分布精度， 由于一个prozone现象是通过与concn比较来纠正的。 伴随着非稀释样品中试剂的分析，即使试剂浓度 可以进行高精度的分析。

公开(公告)号：JPS59176677A

公开(公告)日：1984-10-06

申请号：JP5072683

申请日：1983-03-26

Applicant: Shimadzu Corp

Inventor： YAMAMOTO HIDEKI

IPC: G01N35/02

CPC classification number: G01N35/021

Abstract: PURPOSE:To dispense a sample and >=1 kind of reagent through one dispenser by controlling respective moving mechanisms for that a specific sample and a reagent are dispensed to a reaction pipe according to an analysis item. CONSTITUTION:A device is provided with a moving mechanism 2 mounting the reaction pipe 1, moving mechanism 4 mounting a sample container 3, washing tank 10, moving mechanism 8 mounting a reagent container 7, driving mechanism 12 for the dispenser 11, mechanism 9 for moving the nozzle 14 of the dispenser 11, and control mechanism 13 for respective mechanisms, and they are put in operation according to an in-use mode depending upon an analyzing method. Then, the sample and the 1st reagent are dispensed at a position B and the 2nd sample or reagent is dispensed at a dispensation position E. The 1st reagent is dispensed at the position B and the sample is dispensed at the position E; and the sample and the 1st reagent are dispensed at the position B and the 2nd reagent is dispensed at the position E.

Abstract translation: 目的：根据分析项目，通过控制相应的移动机构将特定样品和试剂分配到反应管中，通过一个分配器分配样品和> = 1种试剂。 构成：装置设置有安装反应管1的移动机构2，安装样品容器3的移动机构4，清洗槽10，安装试剂容器7的移动机构8，分配器11的驱动机构12，机构9 移动分配器11的喷嘴14和用于各个机构的控制机构13，并且根据分析方法，它们根据使用模式投入运行。 然后，将样品和第一试剂分配到位置B，将第二试样或试剂分配到分配位置E.第一试剂在位置B分配，样品在位置E分配; 并且样品和第一试剂在位置B处分配，并且第二试剂在位置E被分配。

公开(公告)号：JPS5932868A

公开(公告)日：1984-02-22

申请号：JP14364782

申请日：1982-08-18

Applicant: Shimadzu Corp

Inventor： YAMAMOTO HIDEKI

IPC: H01M4/08 ,  G01N35/02 ,  G01N35/10

CPC classification number: G01N35/10

Abstract: PURPOSE:To improve the distribution capacity using reagent distributors the number thereof is less than that corresponding to the number of reagents to eliminate idle reaction tubes by selectively sucking a plurality of different kinds of reagents separately with each of the reagent distributors as desired to enable discharge into any reaction tube. CONSTITUTION:Reagents a1(13), a2(14)... are set on a turntable A and selected with the rotation of the turntable A. The reagents are sucked with the reagent distributors 11 and then, reagent nozzles 12 having sucked the reagents move on trains of the reaction tubes which compose a plurality of channels sequentially being fed to the reagent distribution position to discharge the reagents into any reaction tube 10. The reagent nozzles 12 are moved into a cleaning tank 40 to clean. In turntable A, B...I series systems, the suction of reagents, the movement of nozzles, discharge of reagents and cleaning of nozzles, these actions in the distribution can be performed in parallel with one another under the control with a controller.

Abstract translation: 目的：为了提高使用试剂分配器的分配能力，其数量小于通过根据需要选择性地抽吸多种不同种类的试剂以分别使用各种试剂分配器来消除空闲反应管的试剂数量，使其能够排出 进入任何反应管。 构成：将试剂a1（13），a2（14）...设置在转盘A上并通过转盘A的旋转选择。试剂被试剂分配器11吸入，然后将试剂吸嘴12吸入试剂 在构成多个通道的反应管的列上依次送入反应物分配位置，将试剂排放到任何反应管10中。将试剂喷嘴12移入清洗槽40进行清洗。 在转盘A，B ... I系列系统中，试剂的抽吸，喷嘴的移动，试剂的排出和喷嘴的清洁，分配中的这些动作可以在控制器的控制下彼此并行进行。

公开(公告)号：JPH0843402A

公开(公告)日：1996-02-16

申请号：JP17831294

申请日：1994-07-29

Applicant: SHIMADZU CORP

Inventor： KADOTA TOSHIMI ,  YAMAMOTO HIDEKI

IPC: G01N35/02 ,  G01N35/04 ,  G01N35/10

Abstract: PURPOSE:To lower the temperature of exhaust heat produced from a cold reserving means for insulating a reagent tray or a sample tray from heat. CONSTITUTION:The heat produced from a thermomodule 11 provided on the lower side of a reagent tray 9 is exhausted outside an instrument by a fin 12 and a fan 13 through an exhaust heat pipe 14. In the exhaust heat pipe 14, drainage from washing water for washing a pipette probe 7, constant temperature water overflowing a thermostat 3 and dew water of the reagent tray 9 flows through drainpipes 5, 5' and 5'', a drain tray 15 and a drain tube 16. Accordingly, the heat in the exhaust heat pipe 14 is subjected to heat exchange in the drain tube 16 and the temperature thereof lowers.