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    A QPCR ANALYSIS APPARATUS
    11.
    发明申请
    A QPCR ANALYSIS APPARATUS 审中-公开
    QPCR分析仪器

    公开(公告)号：WO2008038259A1

    公开(公告)日：2008-04-03

    申请号：PCT/IE2007/000089

    申请日：2007-09-27

    Applicant: STOKES BIO LIMITED ,  DAVIES, Mark ,  DALY, John ,  DALTON, Tara

    Inventor： DAVIES, Mark ,  DALY, John ,  DALTON, Tara

    IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/00

    CPC classification number: B01L7/525 ,  B01L3/508 ,  B01L7/54 ,  B01L2300/0654 ,  B01L2300/0809 ,  B01L2300/1827 ,  B01L2400/0442 ,  B01L2400/0445 ,  G01N21/6428 ,  G01N2021/6439 ,  G01N2201/062

    Abstract: An apparatus (1) is for DNA amplification with quantitative measurements. A biological sample is held in a cell (2) for the amplification, the cell (2) defining a single space within which the sample rotates. On one side a copper heater (3) is located to supply heat to the cell (2), and on the other side there is a cooling copper block (4) withdrawing heat from the cell. The locations of the heater (3) and the cooling block (4) generate a natural convection loop internally within the cell (2) without need for active cooling - the block (4) passively cooling by withdrawing heat from the direction of the heater (3).A detector (9, 27) captures readings in real time and a processor (10) generates an S-curve for change of sample emission with time. The S-curve (Figs. 4 and 5) also includes a thermal cycle number corresponding to the time parameter, so that the S-curve is given in the traditional qPCR intensity vs. cycle number.

    Abstract translation: 装置（1）用于通过定量测量进行DNA扩增。 将生物样品保持在用于扩增的细胞（2）中，细胞（2）限定样品旋转的单个空间。 在一侧，铜加热器（3）被定位成向电池（2）供应热量，另一侧有一个冷却铜块（4）从电池中取出热量。 加热器（3）和冷却块（4）的位置在电池（2）内部产生自然对流回路，而不需要主动冷却 - 通过从加热器的方向抽出热量（4）被动地冷却 检测器（9,27）实时捕获读数，并且处理器（10）产生用于随时间改变样品发射的S曲线。 S曲线（图4和5）还包括对应于时间参数的热循环数，使得S曲线在传统的qPCR强度与循环数之间给出。

    MICROFLUIDIC CONNECTOR
    12.
    发明申请
    MICROFLUIDIC CONNECTOR 审中-公开
    微流连接器

    公开(公告)号：WO2008038258A1

    公开(公告)日：2008-04-03

    申请号：PCT/IE2007/000088

    申请日：2007-09-27

    Applicant: STOKES BIO LIMITED ,  DAVIES, Mark ,  DALTON, Tara

    Inventor： DAVIES, Mark ,  DALTON, Tara

    IPC: B01L3/00

    CPC classification number: F16L41/18 ,  B01L3/5027 ,  B01L3/5635 ,  B01L3/565 ,  B01L2200/027 ,  F16L39/00 ,  Y10T137/0318

    Abstract: A microfluidic connector (1) comprises an enclosure (6, 7), a fluidic inlet port (2) and a fluidic outlet port (3), in the enclosure, in which the inlet and outlet ports (2, 3) are movable with respect to each other, for example, mutual spacing between the inlet and outlet ports (2, 3) is variable. A port (2) is in a fixed part (6) of the enclosure, and another port (3) is in a part (7) of the enclosure which slides with respect to the fixed part. There may be multiple inlet ports (22, 23) and/or multiple outlet ports (24, 25). Also, there may be an auxiliary port (45) for introduction of fluid into the enclosure (47, 48) or removal of fluid from the enclosure.

    Abstract translation: 微流体连接器（1）包括外壳中的外壳（6,7），流体入口端口（2）和流体出口端口（3），入口端口和出口端口（2,3）可通过外壳 彼此相对，例如，入口端口（3）之间的相互间隔是可变的。 端口（2）位于外壳的固定部分（6）中，另一个端口（3）位于外壳的相对于固定部分滑动的部分（7）中。 可以有多个入口端口（22,23）和/或多个出口端口（24,25）。 而且，可以存在用于将流体引入外壳（47,48）或从外壳移除流体的辅助端口（45）。

    A LIQUID BRIDGE AND SYSTEM
    13.
    发明申请
    A LIQUID BRIDGE AND SYSTEM 审中-公开
    液桥和系统

    公开(公告)号：WO2007091228A1

    公开(公告)日：2007-08-16

    申请号：PCT/IE2007/000013

    申请日：2007-02-07

    Applicant: STOKES BIO LIMITED ,  DAVIES, Mark ,  DALTON, Tara ,  CURRAN, Kieran

    Inventor： DAVIES, Mark ,  DALTON, Tara ,  CURRAN, Kieran

    IPC: B01F13/00 ,  B01L3/00 ,  G01N35/08

    CPC classification number: B01L3/502784 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0037 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/082 ,  C12Q1/686 ,  F17D1/08 ,  Y10T137/87676 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

    Abstract: A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary- suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.

    Abstract translation: 桥（30）包括在毛细管端部的第一入口（31），作为毛细管端部的较窄的第二入口（32），作为毛细管的端部的出口（33） 和用于硅油的室（34）。 油与反应器液滴密度匹配，使得在室（34）内产生中性浮力的环境。 室内的油通过分离反应器液滴的油不断补充。 这使得液滴在进入室（34）时呈现稳定的毛细管悬浮球形。 球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。 从第二入口端口（32）引入第二液滴导致形成不稳定的索道桥，其从较细的第二入口（32）快速破裂，并且液滴在液体桥（30）处结合。 在另一个实施例中，液滴（55）分段成较小的液滴，桥接入口和出口之间的间隙。

    SAMPLING DEVICE
    18.
    发明公开
    SAMPLING DEVICE 审中-公开
    采样装置

    公开(公告)号：EP2553420A2

    公开(公告)日：2013-02-06

    申请号：EP11760345.6

    申请日：2011-03-25

    Applicant: Stokes Bio Limited

    Inventor： DAVIES, Mark ,  DALTON, Tara ,  SAYERS, Michael ,  CHAWKE, Brian

    IPC: G01N1/10 ,  G01N35/10 ,  B01L3/00

    CPC classification number: B01L3/021 ,  B01L3/0293 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  Y10T436/25 ,  Y10T436/25625

    Abstract: The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfludic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.

    Abstract translation: 本发明一般涉及用于在不将气体引入微流体系统（例如液体桥系统）中的情况下获取和/或分配样品的装置，系统和方法。 示例性实施例提供了一种采样装置，其包括外护套; 所述护套内的多个管，其中所述管中的至少一个获取样本，并且至少一个管排出与所述样本不混溶的流体，其中获取所述样本的所述至少一个管可延伸超过 护套的远端并且可缩回到护套内; 以及连接到护套远端部分的阀，当管延伸超过远端时阀门打开，并且当管缩回到护套内时关闭。

    SAMPLING DEVICE
    20.
    发明公开
    SAMPLING DEVICE 审中-公开
    取样装置

    公开(公告)号：EP2432597A2

    公开(公告)日：2012-03-28

    申请号：EP10750161.1

    申请日：2010-05-10

    Applicant: Stokes Bio Limited

    Inventor： DAVIES, Mark ,  DALTON, Tara ,  SAYERS, Michael ,  CHAWKE, Brian

    IPC: B01L99/00

    CPC classification number: B01L3/0293 ,  B01L3/021 ,  B01L7/52 ,  B01L2200/0642

    Abstract: The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including: a sampling member (101) for acquiring or dispensing a sample; and a supply (106) of a fluid that is immiscible with the sample; in which the device is configured to provide a continuous flow of immiscible fluid enveloping the sampling member (101).

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