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    DEVICES, SYSTEMS, AND METHODS FOR AMPLIFYING NUCLEIC ACIDS
    2.
    发明申请
    DEVICES, SYSTEMS, AND METHODS FOR AMPLIFYING NUCLEIC ACIDS 审中-公开
    用于放大核酸的装置，系统和方法

    公开(公告)号：WO2011119994A2

    公开(公告)日：2011-09-29

    申请号：PCT/US2011/030047

    申请日：2011-03-25

    Applicant: STOKES BIO LIMITED ,  DAVIES, Mark ,  DALTON, Tara

    Inventor： DAVIES, Mark ,  DALTON, Tara

    IPC: C12M1/38 ,  C12Q1/68

    CPC classification number: B01L7/525 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2400/0457 ,  B01L2400/0487 ,  C12Q1/6851 ,  C12Q2565/629 ,  C12Q2561/113

    Abstract: The present invention generally relates to a devices, systems, and methods for amplifying nucleic acids in flowing droplets. In certain embodiments, the invention provides a device for amplifying nucleic acids including at least one channel through which sample droplets including nucleic acids flow, in which the nucleic acids in the droplets are optically detectable while the droplets are flowing through the channel, and a plurality of temperature zones in thermal contact with the channel, in which the zones are located at different locations along the channel and the zones are separated from each other.

    Abstract translation: 本发明一般涉及用于扩散流动液滴中的核酸的装置，系统和方法。 在某些实施方案中，本发明提供了用于扩增核酸的装置，所述装置包括至少一个通道，通过该通道，包含核酸的样品液滴流过，其中液滴中的核酸在液滴流过通道时是可光学检测的， 的温度区与通道热接触，其中区域沿着通道位于不同的位置，并且区域彼此分离。

    A MICROFLUIDIC DROPLET QUEUING NETWORK
    5.
    发明申请
    A MICROFLUIDIC DROPLET QUEUING NETWORK 审中-公开
    一个微型流氓排球网络

    公开(公告)号：WO2007091229A1

    公开(公告)日：2007-08-16

    申请号：PCT/IE2007/000014

    申请日：2007-02-07

    Applicant: STOKES BIO LIMITED ,  DAVIES, Mark ,  DALTON, Tara ,  GARVEY, Julie ,  CURRAN, Kieran ,  CURTIN, Damian

    Inventor： DAVIES, Mark ,  DALTON, Tara ,  GARVEY, Julie ,  CURRAN, Kieran ,  CURTIN, Damian

    IPC: B01F13/00 ,  B01L3/00 ,  G01N35/08

    CPC classification number: B01J19/0046 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01J2219/00313 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2400/0487 ,  Y10T436/25

    Abstract: A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) ("aqueous phase") flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow (from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.

    Abstract translation: 多端口液体桥（1）将包含油相载体液体（11）中的水相液滴（10）加入到通风道（4,6）。 室（3）连接四个端口，在使用时永久充满油（11）。 油相在进气口（4）的进料流中进料，并通过排气口（6）和补偿流通口（7）排出。 油载体和样品液滴（“水相”）以相当的流体流通过入口端口（5）流过补偿口（7）。 桥梁（1）的端口由保持在塑料壳体中的位置的毛细管的端部形成。 这些阶段是密度匹配的，以创造引力几乎可忽略的环境。 这导致当从毛细管尖端悬浮时，液滴（10）采用球形。 此外，质量流量的相等性等于体积流量的相等。 入口流（从液滴入口端口5和进气口4）的相位用于确定出口（6）的流动相位。

    A MICROFLUIDIC ANALYSIS SYSTEM
    6.
    发明申请
    A MICROFLUIDIC ANALYSIS SYSTEM 审中-公开
    微流感分析系统

    公开(公告)号：WO2007091230A1

    公开(公告)日：2007-08-16

    申请号：PCT/IE2007/000015

    申请日：2007-02-07

    Applicant: STOKES BIO LIMITED ,  DAVIES, Mark ,  DALTON, Tara

    Inventor： DAVIES, Mark ,  DALTON, Tara

    IPC: B01L7/00 ,  B01L3/00 ,  G01N35/08

    CPC classification number: B01L7/525 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2400/0487 ,  C12Q1/686 ,  G01N21/6428 ,  G01N2201/0826 ,  G01N2201/0833

    Abstract: A thermal cycling device (3) device a number of fixed thermal zones (11, 12, 13) and a fixed conduit (10) passing through the thermal zones. A controller maintains each thermal zone including its section of conduit (10) at a constant temperature. A series of droplets flows through the conduit (10) so that each droplet is thermally cycled, and a detection system detects fluorescence from droplets at all of the thermal cycles. The conduit is in a single plane, and so a number of thermal cycling devices may be arranged together to achieve parallelism. The flow conduit comprises a channel (17) and a capillary tube (10) inserted into the channel. The detection system may perform scans along a direction to detect radiation from a plurality of cycles in a pass.

    Abstract translation: 热循环装置（3）装置设有多个固定热区（11,12,13）和通过热区的固定导管（10）。 控制器将每个热区域保持在其恒定温度下的导管（10）部分。 一系列液滴流过导管（10），使得每个液滴热循环，并且检测系统在所有热循环中检测来自液滴的荧光。 导管处于单个平面中，因此可以将多个热循环装置布置在一起以实现平行。 流动管道包括插入通道中的通道（17）和毛细管（10）。 检测系统可以沿着一个方向执行扫描，以检测通过中多个周期的辐射。

    SAMPLING DEVICE
    9.
    发明申请
    SAMPLING DEVICE 审中-公开
    采样设备

    公开(公告)号：WO2011120004A2

    公开(公告)日：2011-09-29

    申请号：PCT/US2011/030073

    申请日：2011-03-25

    Applicant: STOKES BIO LIMITED ,  DAVIES, Mark ,  DALTON, Tara ,  SAYERS, Michael ,  CHAWKE, Brian

    Inventor： DAVIES, Mark ,  DALTON, Tara ,  SAYERS, Michael ,  CHAWKE, Brian

    IPC: G01N1/10 ,  G01N35/10 ,  B01L3/00

    CPC classification number: B01L3/021 ,  B01L3/0293 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  Y10T436/25 ,  Y10T436/25625

    Abstract: The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfludic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.

    Abstract translation: 本发明一般涉及用于获取和/或分配样品而不将气体引入微流体系统（如液桥系统）中的装置，系统和方法。 示例性实施例提供了一种包括外护套的取样装置; 护套内的多个管，其中至少一个管获取样品，并且至少一个管排出与样品不混溶的流体，其中获取样品的至少一个管可以延伸超过 护套的远端并伸缩到护套内; 以及连接到所述护套的远端部分的阀，当所述管延伸超过所述远端时所述阀打开，并且当所述管缩回到所述护套内时所述阀闭合。

    METHODS AND DEVICES FOR DIGITAL PCR
    10.
    发明申请
    METHODS AND DEVICES FOR DIGITAL PCR 审中-公开
    数字PCR的方法和设备

    公开(公告)号：WO2010018465A2

    公开(公告)日：2010-02-18

    申请号：PCT/IB2009/007568

    申请日：2009-08-11

    Applicant: STOKES BIO LIMITED ,  DAVIES, Mark ,  DALTON, Tara

    Inventor： DAVIES, Mark ,  DALTON, Tara

    CPC classification number: C12Q1/6851 ,  B01L3/502784 ,  B01L7/525 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2400/0487 ,  C12Q2561/101 ,  C12Q2563/159 ,  C12Q2565/629

    Abstract: The invention provides methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a "droplet-in-oil" technology. In the methods, the starting sampled is segmented at least partially into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.

    Abstract translation: 本发明提供了进行核酸反应的方法，包括使用“油滴技术”进行数字PCR的方法。 在所述方法中，起始取样至少部分地分成一组样品液滴，每个样品液滴平均含有约一个或更少个靶核酸拷贝。 液滴通过穿过热循环仪的通道在不混溶的载体流体的连续流中通过，从而靶扩增。 在一个实施方案中，液滴各自为约350nl，并且在近饱和点处计数积极扩增的液滴的数量。

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