公开(公告)号：DK161205C

公开(公告)日：1991-11-25

申请号：DK29883

申请日：1983-01-26

Applicant: UCB SA

Inventor： DEGELAEN JACQUES ,  LOFFET ALBERT ,  DURIEUX JEAN-PIERRE

IPC: G01N33/50 ,  C12N9/52 ,  C12N11/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/37 ,  C12R1/03 ,  G01N33/04

Abstract: An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyze it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtain the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.

公开(公告)号：DK29883D0

公开(公告)日：1983-01-26

申请号：DK29883

申请日：1983-01-26

Applicant: UCB SA

Inventor： DEGELAEN JACQUES ,  LOFFET ALBERT ,  DURIEUX JEAN-PIERRE

IPC: G01N33/50 ,  C12N9/52 ,  C12N11/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/37 ,  C12R1/03 ,  G01N33/04 ,  C12Q

Abstract: An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyze it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtain the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.

公开(公告)号：RO69885A

公开(公告)日：1981-11-04

申请号：RO8686776

申请日：1976-07-07

Applicant: UCB SA

Inventor： LOFFET ALBERT

IPC: A61K38/00 ,  A61P3/00 ,  C07D207/12 ,  C07D207/16 ,  C07K5/06 ,  C07K5/078

Abstract: L-pyroglutamyl-L-prolinamide is useful for correcting metabolic or endocrinal disorders connected with senescence. It can be prepared by reacting L-pyroglutamic acid with L-prolinamide.

公开(公告)号：HU174650B

公开(公告)日：1980-02-28

申请号：HUUE000074

申请日：1976-07-08

Applicant: UCB SA

Inventor： LOFFET ALBERT

IPC: A61K38/00 ,  A61P3/00 ,  C07D207/12 ,  C07D207/16 ,  C07K5/06 ,  C07K5/078 ,  C07C103/52

Abstract: L-pyroglutamyl-L-prolinamide is useful for correcting metabolic or endocrinal disorders connected with senescence. It can be prepared by reacting L-pyroglutamic acid with L-prolinamide.

公开(公告)号：DK161205B

公开(公告)日：1991-06-10

申请号：DK29883

申请日：1983-01-26

Applicant: UCB SA

Inventor： DEGELAEN JACQUES ,  LOFFET ALBERT ,  DURIEUX JEAN-PIERRE

IPC: G01N33/50 ,  C12N9/52 ,  C12N11/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/37 ,  C12R1/03 ,  G01N33/04

Abstract: An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyze it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtain the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.

公开(公告)号：BG42679A3

公开(公告)日：1988-01-15

申请号：BG5946583

申请日：1983-01-26

Applicant: UCB SA

Inventor： DEGELAEN JACQUES ,  LOFFET ALBERT ,  DURIEUX JEAN-PIERRE

IPC: G01N33/50 ,  C12N9/52 ,  C12N11/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/37 ,  C12R1/03 ,  G01N33/04

Abstract: An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyze it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtain the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.

公开(公告)号：AU559670B2

公开(公告)日：1987-03-19

申请号：AU1096483

申请日：1983-02-01

Applicant: UCB SA

Inventor： DEGELAEN JACQUES ,  LOFFET ALBERT

IPC: G01N33/50 ,  C12N9/52 ,  C12N11/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/37 ,  C12R1/03 ,  G01N33/04 ,  C12Q1/26 ,  C12Q1/36

Abstract: An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyze it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtain the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.

公开(公告)号：DE3363319D1

公开(公告)日：1986-06-12

申请号：DE3363319

申请日：1983-01-31

Applicant: UCB SA

Inventor： DEGELAEN JACQUES ,  LOFFET ALBERT ,  DURIEUX JEAN-PIERRE

IPC: G01N33/50 ,  C12N9/52 ,  C12N11/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/37 ,  C12R1/03 ,  G01N33/04

Abstract: An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyze it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtain the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.

公开(公告)号：AT19654T

公开(公告)日：1986-05-15

申请号：AT83870007

申请日：1983-01-31

Applicant: UCB SA

Inventor： DEGELAEN JACQUES ,  LOFFET ALBERT ,  DURIEUX JEAN-PIERRE

IPC: G01N33/50 ,  C12N9/52 ,  C12N11/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/37 ,  C12R1/03 ,  G01N33/04