公开(公告)号：EP1623227A4

公开(公告)日：2009-03-04

申请号：EP04716140

申请日：2004-03-01

Applicant: UNIV MARYLAND

Inventor： TOLOSA LEAH ,  RAO GOVIND ,  GE XUDONG

IPC: G01N33/542 ,  C12N20060101 ,  G01N33/558

CPC classification number: G01N33/542 ,  Y10T436/143333 ,  Y10T436/144444 ,  Y10T436/17

Abstract: A protein sensing molecule is capable of binding an analyte in a sample. The protein sensing molecule includes a first detectable quality that changes in a concentration dependent manner when the protein sensing molecule is bound to the analyte. The protein sensing molecule also includes a second detectable quality that does not undergo substantial change when the protein sensing molecule is bound to the analyte. The protein sensing molecule may be used in methods for characterizing samples and may also be used in sensors.

公开(公告)号：DK1098658T3

公开(公告)日：2009-11-09

申请号：DK99935583

申请日：1999-07-15

Applicant: UNIV MARYLAND

Inventor： LAKOWICZ JOSEPH R ,  RAO GOVIND ,  TOLOSA LEAH ,  EICHHORN LISA

IPC: A61K38/00 ,  C07K16/00 ,  C12Q1/48 ,  C12Q1/54 ,  G01N27/26 ,  G01N33/542 ,  G01N33/66 ,  G01N33/68

Abstract: Disclosed are methods and sensors for detecting the presence or concentration of an analyte in a sample, preferably a sugar such as glucose, which preferably utilizes a labeled engineered protein, or fragment thereof, that is capable of binding the analyte to be detected.

公开(公告)号：AU2004239208A1

公开(公告)日：2004-11-25

申请号：AU2004239208

申请日：2004-03-01

Applicant: UNIV MARYLAND

Inventor： RAO GOVIND ,  GE XUDONG ,  TOLOSA LEAH

IPC: G01N33/558 ,  C12N20060101

Abstract: A protein sensing molecule is capable of binding an analyte in a sample. The protein sensing molecule includes a first detectable quality that changes in a concentration dependent manner when the protein sensing molecule is bound to the analyte. The protein sensing molecule also includes a second detectable quality that does not undergo substantial change when the protein sensing molecule is bound to the analyte. The protein sensing molecule may be used in methods for characterizing samples and may also be used in sensors.

公开(公告)号：AU759888B2

公开(公告)日：2003-05-01

申请号：AU5103399

申请日：1999-07-15

Applicant: UNIV MARYLAND

Inventor： LAKOWICZ JOSEPH R ,  TOLOSA LEAH ,  EICHHORN LISA ,  RAO GOVIND

IPC: C12Q1/48 ,  C12Q1/54 ,  G01N33/542 ,  G01N33/66 ,  G01N33/68 ,  A61K38/00 ,  C07K16/00 ,  G01N27/26

Abstract: Disclosed are methods and sensors for detecting the presence or concentration of an analyte in a sample, preferably a sugar such as glucose, which preferably utilizes a labeled engineered protein, or fragment thereof, that is capable of binding the analyte to be detected.

公开(公告)号：ES2329850T3

公开(公告)日：2009-12-01

申请号：ES99935583

申请日：1999-07-15

Applicant: UNIV MARYLAND

Inventor： LAKOWICZ JOSEPH R ,  TOLOSA LEAH ,  EICHHORN LISA ,  RAO GOVIND

IPC: A61K38/00 ,  C07K16/00 ,  C12Q1/48 ,  C12Q1/54 ,  G01N27/26 ,  G01N33/542 ,  G01N33/66 ,  G01N33/68

Abstract: Un procedimiento para determinar la presencia o concentración de un analito en una muestra, que comprende las etapas de: a) proporcionar una molécula de detección de proteínas que puede unir dicho analito en dicha muestra, teniendo dicha molécula una calidad detectable que cambia de un modo dependiente de la concentración cuando dicha molécula se une a dicho analito; b) exponer dicha molécula de detección a dicha muestra; y c) medir cualquier cambio en dicha calidad detectable para así determinar la presencia o concentración de dicho analito en dicha muestra, en el que dicha molécula de detección de proteínas es una proteína de unión a glucosa/galactosa modificada que contiene un residuo de cisteína en las posiciones 26 y 182 y en el que un colorante donante reactivo con tiol y un colorante aceptor reactivo con tiol están unidos a dichos residuos de cisteína respectivamente en las posiciones 26 y 182.

公开(公告)号：AT435654T

公开(公告)日：2009-07-15

申请号：AT99935583

申请日：1999-07-15

Applicant: UNIV MARYLAND

Inventor： LAKOWICZ JOSEPH ,  TOLOSA LEAH ,  EICHHORN LISA ,  RAO GOVIND

IPC: A61K38/00 ,  C07K16/00 ,  C12Q1/48 ,  C12Q1/54 ,  G01N27/26 ,  G01N33/542 ,  G01N33/66 ,  G01N33/68

Abstract: Disclosed are methods and sensors for detecting the presence or concentration of an analyte in a sample, preferably a sugar such as glucose, which preferably utilizes a labeled engineered protein, or fragment thereof, that is capable of binding the analyte to be detected.

公开(公告)号：CA2336985A1

公开(公告)日：2000-01-27

申请号：CA2336985

申请日：1999-07-15

Applicant: UNIV MARYLAND

Inventor： LAKOWICZ JOSEPH R ,  EICHHORN LISA ,  RAO GOVIND ,  TOLOSA LEAH

IPC: C12Q1/48 ,  C12Q1/54 ,  G01N33/542 ,  G01N33/66 ,  G01N33/68 ,  G01N27/26

Abstract: Disclosed are methods and sensors for detecting the presence or concentratio n of an analyte in a sample, preferably a sugar such as glucose, which preferably utilizes a labeled engineered protein, or fragment thereof, that is capable of binding the analyte to be detected.

公开(公告)号：CA2336985C

公开(公告)日：2011-01-04

申请号：CA2336985

申请日：1999-07-15

Applicant: UNIV MARYLAND

Inventor： LAKOWICZ JOSEPH R ,  TOLOSA LEAH ,  EICHHORN LISA ,  RAO GOVIND

IPC: C12Q1/54 ,  C12Q1/48 ,  G01N27/26 ,  G01N33/542 ,  G01N33/66 ,  G01N33/68

Abstract: A method for determining the presence or concentration of a glucose or galactose analyte in a sample, comprises providing a sensing molecule that is a protein capable of binding the analyte in said sample. The sensing molecule is a glucose/galactose binding protein that is modified to contain a cysteine residue at positions 26 and 182 of said molecule. The sensing molecule comprises an energy donor moiety and an energy acceptor moiety, each bound to the sensing molecule and spaced such that there is a detectable signal change when the sensing molecule is bound to the analyte. The sensing molecule is then exposed to said sample and the detectable signal change is measured to thereby determine the presence or concentration of said analyte in said sample.