公开(公告)号：WO2014106189A2

公开(公告)日：2014-07-03

申请号：PCT/US2013078328

申请日：2013-12-30

Applicant: CONAGEN INC ,  GIVAUDAN SA

Inventor： ZHOU RUI ,  BHUIYA MOHAMMAD WADUD ,  CAI XIANPENG ,  YU XIAODAN ,  EILERMAN ROBERT G

IPC: C12P7/24

CPC classification number: C12P7/24 ,  C12N9/1007 ,  C12P7/42 ,  C12Y201/01068

Abstract: This disclosure provides a method of making vanillin including expressing 4- hydroxyphenylacetate 3-hyrdroxylase, caffeic acid 3-O-methyltransferase, methionine synthase, feruloyl-CoA synthetase, and enoyl-CoA hydratase/aldolase in a mixture, feeding p-coumaric acid to the mixture, and collecting vanillin. It further includes an enzyme, such as caffeic acid 3-O-methyltransferase, that facilitates the increased conversion of caffeic acid to ferulic acid, wherein the enzyme has been modified at a residue that allows for increased methylation of ferulic acid, and a method of using the enzyme in the making of ferulic acid followed by vanillin.

Abstract translation: 本公开提供了制备香草醛的方法，包括在混合物中表达4-羟基苯基乙酸酯3-羟基乙酸酯，咖啡酸3-O-甲基转移酶，甲硫氨酸合成酶，阿魏酰辅酶A合成酶和烯酰辅酶A水合酶/醛缩酶，将对香豆酸 混合物，并收集香草醛。 它还包括一种酶，如咖啡酸3-O-甲基转移酶，其有助于提高咖啡酸向阿魏酸的转化率，其中该酶已经在允许增加阿魏酸甲基化的残基中被修饰，以及一种方法 使用酶制造阿魏酸，然后用香草醛。

公开(公告)号：WO2014102368A1

公开(公告)日：2014-07-03

申请号：PCT/EP2013/078117

申请日：2013-12-30

Applicant: EVIAGENICS S.A.

Inventor： RAMAEN, Odile ,  SAUVEPLANE, Vincent ,  PANDJAITAN, Rudy

IPC: C12N9/88 ,  C12P7/24 ,  C12N15/52

CPC classification number: C12P7/24 ,  C12N9/0028 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/1007 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12Y105/01036 ,  C12Y106/02004 ,  C12Y114/13002 ,  C12Y114/13007 ,  C12Y114/13011 ,  C12Y201/01068 ,  C12Y301/01073 ,  C12Y402/01 ,  C12Y402/01017 ,  C12Y403/01023 ,  C12Y403/01024 ,  C12Y403/01025 ,  C12Y602/01012

Abstract: The invention relates to a cell comprising heterologous polynucleotides encoding a multienzyme complex involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, which multienzyme complex comprises enzymes for the biosynthesis of coumaric acid and a crotonase.

Abstract translation: 本发明涉及包含编码参与苯丙素代谢途径的多酶复合物的异源多核苷酸的细胞，以及香草醛或其羟基苯甲醛前体的生物合成，所述多酶复合物包含用于生物合成香豆酸和巴豆酸酶的酶。

公开(公告)号：KR1020180005501A

公开(公告)日：2018-01-16

申请号：KR1020160085698

申请日：2016-07-06

Applicant: 한국생명공학연구원

Inventor： 홍영수 ,  강선영 ,  허경택 ,  이재경

IPC: C12N15/63 ,  C12N9/10 ,  C12P7/22

CPC classification number: C12N9/10 ,  C12N15/63 ,  C12P7/22 ,  C12N9/1007 ,  C12Y201/01068 ,  C12Y203/01095 ,  C12Y205/01054 ,  C12Y402/01051 ,  C12Y403/01023 ,  C12Y504/99005 ,  C12Y602/01012 ,  C12Y604/01002

Abstract: 본발명은피노스틸벤또는프테로스틸벤생산용재조합벡터, 피노스틸벤또는프테로스틸벤생산용형질전환체및 피노스틸벤또는프테로스틸벤생산방법에관한것이다. 본발명의피노스틸벤또는프테로스틸벤생산용재조합벡터를이용시, 피노스틸벤또는프테로스틸벤을단일벡터시스템으로수득가능하고, 미생물대사경로를통하여단순당만으로도피노스틸벤또는프테로스틸벤을대량생산하므로경제적이다. 또한, 상기재조합벡터로생산된피노스텔벤또는프테로스틸벤은항암효과, 항염증효과, 항고지혈증효과및 항산화효과를가지므로, 의약산업에유용하게이용될수 있다.

Abstract translation: 本发明涉及一种程序用于询问黑芪或芪重组载体，黑茋或用于询问钢性状生产转化体和Ben黑茋或用于生产茋询问节目制作方法的程序。 驱动黑茋或用于询问茋生产本发明，黑茋或用于通过微生物代谢途径黑茋或用于询问钢程序询问可能，得到二苯乙烯在一个单一的载体系统，只是每简单的程序的重组载体的程序 这是经济的，因为它大量生产本。 此外，由于笨笨黑星状或用于询问与的抗癌作用的重组载体生产的钢的程序，抗炎，抗高脂血症的效果和抗氧化活性，可以有效地在制药工业中使用。

公开(公告)号：KR1020130027338A

公开(公告)日：2013-03-15

申请号：KR1020110090866

申请日：2011-09-07

Applicant: 강원대학교산학협력단

Inventor： 유창연 ,  성은수 ,  유지혜 ,  이재근 ,  김명조 ,  임정대

IPC: A01H5/00 ,  C12N15/82 ,  C12N15/29

CPC classification number: C12N15/8218 ,  C07K14/415 ,  C12N9/1007 ,  C12N15/8243 ,  C12N15/8255 ,  C12N15/8265 ,  C12Y201/01068

Abstract: PURPOSE: COMT gene originated from Miscanthus sinensis and its use is provided to use for the yield increase controlling the anti oxidation activation of plants as the phenolic compound contents are controllable in the vector-transformed plant. CONSTITUTION: The method to control the phenolic compound content of plant comprises a step that controls the revelation of COMT gene by transforming recombinant vector in a plant cell which contains COMT [caffeic acid O-methyltransferase] gene originated from Miscanthus sineis; and a step that redifferentiates plants from the transformed plant cells. The phenolic compound content of plant is reduced by inhibiting the revelation of COMT gene. The phenolic compound contents of plant is increased by the overexpression of COMT gene. The phenolic compound of plant is flavonoid or lignine. The inhibition of COMT gene revelation is executed by transforming the plant with the recombinant plant vector in which COMT gene originated from Miscanthus sinensis is inserted in an anti-sense direction. The COMT gene is formed with a base rank of the rank number 1. The plant with reduced content of phenolic compound is produced by inhibiting the expression of COMT gene.

Abstract translation: 目的：COMT基因起源于芒草，其用途用于控制植物的抗氧化活化的产量增加，因为酚类化合物含量在载体转化植物中是可控的。 构成：控制植物酚类化合物含量的方法包括通过在含有源自芒草的COMT [咖啡酸O-甲基转移酶]基因的植物细胞中转化重组载体来控制COMT基因的启动的步骤; 以及将植物从转化的植物细胞重新分化的步骤。 通过抑制COMT基因的启示来减少植物的酚类化合物含量。 通过COMT基因的过表达，植物的酚类化合物含量增加。 植物的酚类化合物是类黄酮或木质素。 通过用来自中国芒草的COMT基因以反义方向插入的重组植物载体转化植物来进行COMT基因启动的抑制。 COMT基因形成为等级1的碱基级别。通过抑制COMT基因的表达产生酚类化合物含量降低的植物。