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公开(公告)号：KR1020140083473A
公开(公告)日：2014-07-04
申请号：KR1020120153265
申请日：2012-12-26
Applicant: 강원대학교산학협력단
Abstract: The present invention relates to a method for increasing biomass of ginseng adventitious roots, comprising a step of culturing the ginseng adventitious roots through light emitting diode (LED) irradiation at a red light wavelength range of 625-630 nm. The optimum proliferation conditions for culturing the ginseng adventitious roots through a bioreactor is developed by using the irradiation of the LED in a specific wavelength according to the present invention, thereby establishing the formation and growth of the favorable ginseng adventitious roots and the increase in biomass. Thus, provided can be a method for massively propagating the ginseng adventitious roots through tissue culture, capable of industrially mass-producing the ginseng adventitious roots in a bioreactor, thereby producing high value-added ginseng throughout the year without being affected by climatic and environmental conditions, and supplying the ginseng adventitious roots to various user classes at a low price.
Abstract translation: 本发明涉及一种提高人参不定根生物量的方法，其特征在于，包括通过在625-630nm的红光波长范围的发光二极管（LED）照射培养人参不定根的步骤。 通过使用根据本发明的特定波长的LED的照射来开发通过生物反应器培养人参不定根的最佳增殖条件，从而建立有利的人参不定根的形成和生长以及生物量的增加。 因此，提供的方法是通过组织培养大规模地繁殖人参不定根，能够在生物反应器中工业上大量生产人参不定根，从而在不受气候和环境条件影响的情况下在一年中生产高附加值人参 ，以低价格为各种用户提供人参不定根。
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公开(公告)号：KR1020130027338A
公开(公告)日：2013-03-15
申请号：KR1020110090866
申请日：2011-09-07
Applicant: 강원대학교산학협력단
CPC classification number: C12N15/8218 , C07K14/415 , C12N9/1007 , C12N15/8243 , C12N15/8255 , C12N15/8265 , C12Y201/01068
Abstract: PURPOSE: COMT gene originated from Miscanthus sinensis and its use is provided to use for the yield increase controlling the anti oxidation activation of plants as the phenolic compound contents are controllable in the vector-transformed plant. CONSTITUTION: The method to control the phenolic compound content of plant comprises a step that controls the revelation of COMT gene by transforming recombinant vector in a plant cell which contains COMT [caffeic acid O-methyltransferase] gene originated from Miscanthus sineis; and a step that redifferentiates plants from the transformed plant cells. The phenolic compound content of plant is reduced by inhibiting the revelation of COMT gene. The phenolic compound contents of plant is increased by the overexpression of COMT gene. The phenolic compound of plant is flavonoid or lignine. The inhibition of COMT gene revelation is executed by transforming the plant with the recombinant plant vector in which COMT gene originated from Miscanthus sinensis is inserted in an anti-sense direction. The COMT gene is formed with a base rank of the rank number 1. The plant with reduced content of phenolic compound is produced by inhibiting the expression of COMT gene.
Abstract translation: 目的：COMT基因起源于芒草，其用途用于控制植物的抗氧化活化的产量增加，因为酚类化合物含量在载体转化植物中是可控的。 构成：控制植物酚类化合物含量的方法包括通过在含有源自芒草的COMT [咖啡酸O-甲基转移酶]基因的植物细胞中转化重组载体来控制COMT基因的启动的步骤; 以及将植物从转化的植物细胞重新分化的步骤。 通过抑制COMT基因的启示来减少植物的酚类化合物含量。 通过COMT基因的过表达，植物的酚类化合物含量增加。 植物的酚类化合物是类黄酮或木质素。 通过用来自中国芒草的COMT基因以反义方向插入的重组植物载体转化植物来进行COMT基因启动的抑制。 COMT基因形成为等级1的碱基级别。通过抑制COMT基因的表达产生酚类化合物含量降低的植物。
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公开(公告)号：KR101214785B1
公开(公告)日：2012-12-24
申请号：KR1020100037643
申请日：2010-04-23
Applicant: 강원대학교산학협력단
Abstract: 본발명은드라이마리아코다타잎으로부터신초의직접적인고빈도재생방법및 그에따른식물체에관한것으로, 더욱상세하게는드라이마리아코다타() 종자를발아시켜절취한유묘의잎의절편을 NAA; BAP 또는 TDZ; 및수크로스가함유된 MS 배지에서신초를고 빈도(high-frequency)로재생시키는단계를포함하는드라이마리아코다타() 신초의직접적인고빈도재생방법, 상기고빈도로재생된드라이마리아코다타() 신초로부터재분화된드라이마리아코다타식물체의제조방법, 상기방법에따라제조된재분화된드라이마리아코다타식물체및 이의종자를제공한다.
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公开(公告)号：KR101537806B1
公开(公告)日：2015-07-29
申请号：KR1020130098930
申请日：2013-08-21
Applicant: 강원대학교산학협력단
Abstract: 본발명은옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용조성물에관한것으로, 더욱상세하게는옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용조성물, 상기조성물을식물에처리하는단계를포함하는식물의병원균방제또는내성증진방법및 옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용생물농약에관한것으로, 본발명의옻나무추출물은알러지를유발하는독성물질인우루시올(urushiol)이제거되어소비자에게안전하게제공될수 있을뿐만아니라, 식물의초기생육을촉진시켜주고, 재배시문제가되는식물병원균방어관련유전자들이강하게발현되고, 실질적으로바이러스병에대한저항성을나타내므로본 발명의옻나무추출물은사용자에게는안전하면서재배작물에게는유용한식물병원균방제또는내성증진용생물농약으로유용하게사용될수 있다.
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公开(公告)号：KR1020120009653A
公开(公告)日：2012-02-02
申请号：KR1020100069834
申请日：2010-07-20
Applicant: 강원대학교산학협력단
Abstract: PURPOSE: A method for producing hexaploid of Miscanthus X giganteus is provided to improve heterosis and to ensure high biomass productivity. CONSTITUTION: A method for producing heterosis of Miscanthus X gigianteus comprises: a step of sterilizing immature tissue of Miscanthus X gigianteus; a step of culturing the sterilized tissue in a callus induction medium; a step sub-culturing the to select the callus; a step of treating oryzalin to the callus to suppress somatic cell division; a step of culturing and differentiating a shoot; a step of moving the shoot to the MS medium and inducing adventitious roots; and a step of moving the plant into a flowerpot.
Abstract translation: 目的：提供芒草X型六倍体的方法，以改善杂种优势并确保高生物量生产力。 构成：芒草杂种优势的生产方法包括：对芒草X的未成熟组织进行灭菌的步骤; 在愈伤组织诱导培养基中培养灭菌组织的步骤; 选择愈伤组织的步骤; 将谷维素治疗愈伤组织以抑制体细胞分裂的步骤; 培养和分化苗条的步骤; 将芽移动到MS培养基并诱导不定根的步骤; 并将植物移入花盆的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020100122543A
公开(公告)日：2010-11-23
申请号：KR1020090041487
申请日：2009-05-13
Applicant: 강원대학교산학협력단
IPC: A61K36/899 , A61K8/97 , A61Q19/00 , A61P31/04
CPC classification number: Y02A50/473 , Y02A50/481
Abstract: PURPOSE: A Sorghum bicolor Moench extract having an antibacterial activity is provided to prevent side effects by human body toxicity accumulation and to use as an alternative agent of conventional synthetic antibacterial agent. CONSTITUTION: A Sorghum bicolor Moench extract is isolated using low alcohol, methanol. The Sorghum bicolor Moench extract is fractioned using hexane, ethyl acetate, n-butanol or water. A cosmetic composition for anti-bacteria contains the Sorghum bicolor Moench extract as an active ingredient. The composition has antibacterial activities to Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, or Klebsiella pneumoniae. The cosmetic composition is used in the form of skin softener, nutrition skin, nutrition cream, essence, pack or shower powder.
Abstract translation: 目的：提供具有抗菌活性的高粱双色Moench提取物，以防止人体毒性积累的副作用，并用作常规合成抗菌剂的替代试剂。 构成：使用低酒精，甲醇分离高粱双色Moench提取物。 高粱双色Moench提取物用己烷，乙酸乙酯，正丁醇或水分级。 抗菌化妆品组合物含有高粱双色Moench提取物作为活性成分。 该组合物对金黄色葡萄球菌，大肠杆菌，鼠伤寒沙门氏菌或肺炎克雷伯杆菌具有抗菌活性。 化妆品组合物以皮肤柔软剂，营养皮肤，营养霜，精华液，包装或淋浴粉的形式使用。
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公开(公告)号：KR101578720B1
公开(公告)日：2015-12-18
申请号：KR1020120153265
申请日：2012-12-26
Applicant: 강원대학교산학협력단
CPC classification number: Y02P60/149
Abstract: 본발명은삼 식물체부정근에 LED(light emitting diode)를적색광파장인 625~630㎚범위로조사하여배양하는단계를포함하는삼 식물체부정근의바이오매스를증가시키는방법에관한것으로, 본발명에따른특정파장의 LED 조사를이용하여삼 식물체부정근의생물반응기배양을위한최적증식조건의개발을통하여삼 부정근의양호한부정근의형성및 생육과바이오매스의증가를확립함에따라, 생물반응기내삼의부정근의대량생산을산업화하여기후및 환경조건에영향을받지않고고부가가치의삼을년 중생산할수 있으며또한다양한수요층에저렴한가격으로공급할수 있게하는조직배양에의한삼 부정근의대량증식방법을제공할수 있다.
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公开(公告)号：KR1020150098363A
公开(公告)日：2015-08-28
申请号：KR1020140019525
申请日：2014-02-20
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 파라핀 용액을 이용한 삽목묘의 생육을 증진시키기 위한 삽수의 저장방법 및 그에 따른 식물체에 관한 것으로, 상기 방법에 의해 저장된 삽수로 삽목된 식물체는 생육 증가 효과가 있고, 열매 수확량과 과육 수분함량이 증가되어 농가의 수익률를 증가시킬 수 있다.
Abstract translation: 本发明涉及使用石蜡溶液和植物从而存储用于增强根尖切片生长的切屑的方法。 由该方法储存的切屑所切割的植物具有增加生长的效果，并且可以通过增加水果果实的产量和水果的含水量来提高农场的利润率。
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公开(公告)号：KR1020150021673A
公开(公告)日：2015-03-03
申请号：KR1020130098930
申请日：2013-08-21
Applicant: 강원대학교산학협력단
CPC classification number: A01N65/08
Abstract: 본발명은옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용조성물에관한것으로, 더욱상세하게는옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용조성물, 상기조성물을식물에처리하는단계를포함하는식물의병원균방제또는내성증진방법및 옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용생물농약에관한것으로, 본발명의옻나무추출물은알러지를유발하는독성물질인우루시올(urushiol)이제거되어소비자에게안전하게제공될수 있을뿐만아니라, 식물의초기생육을촉진시켜주고, 재배시문제가되는식물병원균방어관련유전자들이강하게발현되고, 실질적으로바이러스병에대한저항성을나타내므로본 발명의옻나무추출물은사용자에게는안전하면서재배작물에게는유용한식물병원균방제또는내성증진용생물농약으로유용하게사용될수 있다.
Abstract translation: 本发明涉及一种用于控制病原体并提高植物耐药性的组合物，其中包括漆树提取物作为有效成分，更具体地说，涉及一种用于控制病原体和提高植物抗性的组合物，其中包括漆树提取物作为有效成分 组分，用于控制病原体并改善植物抗性的方法，包括将组合物处理到植物中的步骤，以及包含漆树提取物的植物中的生物肥料作为有效成分。 本发明的漆树提取物可以安全地提供给消费者，因为去除引起过敏的有毒物质的漆酚，并促进植物的初始饲养。 此外，与植物病原体防御相关的基因，当培养时成为问题，强烈表达，使得提取物实际上显示出对病毒病的抗性。 因此，本发明的漆树提取物对使用者是安全的，并且可以用作用于控制病原体的生物肥料并改善用于农作物的植物的抗性。
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公开(公告)号：KR101272473B1
公开(公告)日：2013-06-17
申请号：KR1020110110371
申请日：2011-10-27
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 (a) 억새 종자를 캘러스 유도 배지에 치상하여 억새 캘러스를 유도하는 단계; (b) 상기 유도된 억새 캘러스와 외래 유전자가 삽입된 식물 발현 벡터를 포함하는 아그로박테리움 튜머파시엔스 (
Agrobacterium
tumefaciens )를 공동배양 배지에서 공동배양하여 형질전환시키는 단계; (c) 상기 공동배양한 식물세포를 항생제가 첨가된 선발 배지에서 선발하는 단계; (d) 상기 선발된 억새 세포를 재분화 개체를 발근시키기 위한 신장 (elongation) 배지에서 신장시키는 단계; 및 (e) 상기 신장된 세포를 발근 유도배지에서 발근을 유도하는 단계를 포함하는 형질전환 억새 식물체의 제조 방법, 상기 방법에 의해 제조된 형질전환 억새 식물체 및 이의 종자에 관한 것이다.
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