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    MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME
    11.
    发明申请
    MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 有权
    具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法

    公开(公告)号：US20090053779A1

    公开(公告)日：2009-02-26

    申请号：US12097099

    申请日：2007-03-14

    Applicant: Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

    Inventor： Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

    IPC: C12P13/08 ,  C12N15/01 ,  C12N15/80 ,  C12N15/81 ,  C12N15/74 ,  C12N1/14 ,  C12N1/15 ,  C12N1/16 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21

    CPC classification number: C12P13/08 ,  C12N9/1014 ,  C12Y201/02011

    Abstract: The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.

    Abstract translation: 本发明涉及具有提高的支链氨基酸生产率的突变微生物，以及使用突变微生物生产支链氨基酸的方法。 更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物 涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。 通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

    Mutant microorganism having improved production ability of branched amino acid and method for preparing branched amino acid using the same
    14.
    发明授权
    Mutant microorganism having improved production ability of branched amino acid and method for preparing branched amino acid using the same 有权
    具有提高支链氨基酸生产能力的突变体微生物及使用其制备支链氨基酸的方法

    公开(公告)号：US08148137B2

    公开(公告)日：2012-04-03

    申请号：US12097099

    申请日：2007-03-14

    Applicant: Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

    Inventor： Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

    IPC: C12N1/21 ,  C12P13/08

    CPC classification number: C12P13/08 ,  C12N9/1014 ,  C12Y201/02011

    Abstract: The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.

    Abstract translation: 本发明涉及具有提高的支链氨基酸生产率的突变微生物，以及使用突变微生物生产支链氨基酸的方法。 更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物 涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。 通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

    Microorganisms and processes for enhanced production of pantothenate
    15.
    发明申请
    Microorganisms and processes for enhanced production of pantothenate 有权
    用于增加泛酸盐生产的微生物和方法

    公开(公告)号：US20040091979A1

    公开(公告)日：2004-05-13

    申请号：US10466641

    申请日：2003-07-18

    Inventor： Rogers R. Yocum ,  Thomas A. Patterson ,  Janice G. Pero ,  Theron Hermann

    IPC: C12P013/04 ,  C12N001/21

    CPC classification number: C12N9/0006 ,  C12N9/1014 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/93 ,  C12P13/02 ,  C12Y201/02011 ,  C12Y401/01011 ,  C12Y603/02001

    Abstract: The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate by enzymes that contribute to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

    Abstract translation: 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加关键中间体水平，通过有助于其合成的酶的酮托品来提高所需产物的生产的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

    MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME
    16.
    发明申请
    MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 审中-公开
    具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法

    公开(公告)号：WO2008088104A1

    公开(公告)日：2008-07-24

    申请号：PCT/KR2007/001263

    申请日：2007-03-14

    Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  LEE, Sang Yup ,  PARK, Jin Hwan ,  LEE, Kwang Ho ,  KIM, Tae Yong

    Inventor： LEE, Sang Yup ,  PARK, Jin Hwan ,  LEE, Kwang Ho ,  KIM, Tae Yong

    IPC: C12N1/16

    CPC classification number: C12P13/08 ,  C12N9/1014 ,  C12Y201/02011

    Abstract: The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.

    Abstract translation: 本发明涉及具有提高的支链氨基酸生产力的突变微生物，以及使用突变微生物生产支链氨基酸的方法。 更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物 涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。 通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

    A METHOD TO BY-PASS ALLOSTERIC DOMAIN ACTIVITY OF AN ENZYME SO AS TO ALTER ITS FEEDBACK OR FEED-FORWARD INHIBITION OR ACTIVATION
    17.
    发明申请
    A METHOD TO BY-PASS ALLOSTERIC DOMAIN ACTIVITY OF AN ENZYME SO AS TO ALTER ITS FEEDBACK OR FEED-FORWARD INHIBITION OR ACTIVATION 审中-公开
    一种方法来分析酶的独特的域活性，以提高其反馈或进给的前向抑制或激活

    公开(公告)号：WO2012014228A1

    公开(公告)日：2012-02-02

    申请号：PCT/IN2011/000496

    申请日：2011-07-27

    Applicant: SINGH, Abhishek, Narain

    Inventor： SINGH, Abhishek, Narain

    IPC: C12N9/00 ,  C12P13/02 ,  C12N9/10

    CPC classification number: C12P13/02 ,  C12N9/0006 ,  C12N9/1014 ,  C12N9/88 ,  C12N9/93 ,  C12N15/8216 ,  C12N15/8243 ,  C12Y101/01169 ,  C12Y201/02011 ,  C12Y401/01015 ,  C12Y603/02001

    Abstract: A method to bypass allosteric domain activity of an enzyme so as to alter the feedback/ feed-forward inhibition/ activation, comprises of (i) a method of increasing the downstream metabolic product such in fermentation or for genetically modified crop, where the wild type organism is feedback or feed-forward inhibited at one of its enzymatic reaction by introducing transgene to be expressed in a common cellular compartment such as cytosol and (ii) a method of decreasing the down stream metabolic product such as for therapeutic purpose, such as controlling the uncontrolled growth of tumor, where the wild type organism is feedback or feed-forward activated at one of its enzymatic reaction, by introducing transgene to be expressed in a common cellular compartment cytosol for that enzyme.

    Abstract translation: 绕过酶的变构结构域活性以改变反馈/前馈抑制/活化的方法包括：（i）增加下游代谢产物如发酵或转基因作物的方法，其中野生型 生物体在其酶促反应之一中被反馈或前馈抑制，通过引入待在普通细胞间隔（例如细胞质溶胶）中表达的转基因，和（ii）降低下行代谢产物如治疗目的的方法，例如控制 在其酶促反应之一的情况下，野生型生物反馈或前馈活化的肿瘤的不受控制的生长通过将转基因导入用于该酶的常见细胞间隔胞溶胶中而被表达。

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