公开(公告)号：US20130092821A1

公开(公告)日：2013-04-18

申请号：US13695267

申请日：2011-04-25

Applicant: Aydogan Ozcan ,  Ahmet F. Coskun ,  Ikbal Sencan ,  Ting-Wei Su

Inventor： Aydogan Ozcan ,  Ahmet F. Coskun ,  Ikbal Sencan ,  Ting-Wei Su

IPC: H01L27/146 ,  G01N21/64

CPC classification number: H01L27/14625 ,  G01N21/64 ,  G01N21/6456 ,  G01N21/648 ,  G01N2021/6471 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2201/0833

Abstract: An imaging device uses a fiber optic faceplate (FOF) with a compressive sampling algorithm for the fluorescent imaging of a sample over an large field-of-view without the need for any lenses or mechanical scanning. The imaging device includes a sample holder configured to hold a sample and a prism or hemispherical glass surface disposed adjacent the sample holder on a side opposite the lower surface of the sample holder. A light source is configured to illuminate the sample via the prism or the hemispherical surface, wherein substantially all of the light is subject to total internal reflection at the lower surface of the sample holder. The FOF is disposed adjacent to the lower surface of the sample holder, the fiber optic array having an input side and an output side. The device includes an imaging sensor array disposed adjacent to the output side of the fiber optic array.

Abstract translation: 成像装置使用具有压缩采样算法的光纤面板（FOF），用于在大视野范围内的样品的荧光成像，而不需要任何透镜或机械扫描。 成像装置包括：样本保持器，其被配置为保持样本和邻近样本保持器设置在与样本保持器的下表面相对的一侧的棱镜或半球形玻璃表面。 光源被配置为经由棱镜或半球形表面照射样品，其中基本上所有的光在样品保持器的下表面处受到全内反射。 FOF设置在样品架的下表面附近，光纤阵列具有输入侧和输出侧。 该装置包括邻近光纤阵列的输出侧设置的成像传感器阵列。

公开(公告)号：US20130070246A1

公开(公告)日：2013-03-21

申请号：US13613463

申请日：2012-09-13

Applicant: Dar-Bin SHIEH ,  Pau-Choo CHUNG

Inventor： Dar-Bin SHIEH ,  Pau-Choo CHUNG

IPC: G01J3/00

CPC classification number: G01N21/255 ,  A61B5/0075 ,  A61B5/6898 ,  A61B2560/0431 ,  A61B2562/046 ,  G01J3/0205 ,  G01J3/0218 ,  G01J3/0264 ,  G01J3/10 ,  G01J3/2803 ,  G01N21/251 ,  G01N21/474 ,  G01N21/49 ,  G01N2021/4742 ,  G01N2201/067 ,  G01N2201/0833

Abstract: An optical detection apparatus used for detecting a tissue includes a light-emitting unit, a spectroscopic unit and a light-sensing array. The light-emitting unit emits light entering into the tissue. The spectroscopic unit receives the light outputted from the tissue and divides the received light into a plurality of rays with different wavelengths. The light-sensing array senses the rays outputted from the spectroscopic unit so as to generate an array spectrum. By the spectroscopic unit, the detection of the rays of multiple wavelengths can be performed without using plural light-emitting diodes for emitting light of different wavelengths. Besides, the user can perceive the detection result (e.g. the location of the abnormal tissue) intuitively by integrating the light-sensing array and the spectroscopic unit.

Abstract translation: 用于检测组织的光学检测装置包括发光单元，分光单元和光感测阵列。 发光单元发射进入组织的光。 分光单元接收从组织输出的光，并将接收的光分成具有不同波长的多个光线。 感光阵列感测从分光单元输出的光线，以产生阵列光谱。 通过分光单元，可以不使用多个用于发射不同波长的光的发光二极管来执行多个波长的光线的检测。 此外，通过集成光感测阵列和分光单元，用户可以直观地感知检测结果（例如异常组织的位置）。

公开(公告)号：US08227719B2

公开(公告)日：2012-07-24

申请号：US12997923

申请日：2009-06-25

Applicant: David A. Prystupa ,  Jennifer Powell ,  Matthew Allen ,  Chris Vogt

Inventor： David A. Prystupa ,  Jennifer Powell ,  Matthew Allen ,  Chris Vogt

IPC: B07C5/342

CPC classification number: B07C5/3425 ,  G01N21/314 ,  G01N21/85 ,  G01N2021/8592 ,  G01N2021/8838 ,  G01N2201/0627 ,  G01N2201/0833 ,  G01N2201/1293 ,  Y10S209/938

Abstract: Fusarium infected grain is separated by comparing reflected and transmitted light at two wavelengths, one at which the light is substantially reflected and scattered the same by healthy and infected kernels, the other at which the light is reflected and scattered to a significantly greater degree by infected than healthy kernels. An apparatus having a rotating apertured cylinder, with a low internal vacuum, allows comparison of individual kernels. When comparison indicates that a kernel is infected, a lever dislodges it from the cylinder allowing it to fall into a receptacle for infected kernels. Kernels remaining on the cylinder are scraped off to fall into a receptacle for healthy kernels. Although results vary, to some extent depending on the degree of infection, approximately 90% of healthy kernels and 5% of infected kernels are deemed “healthy”, while approximately 10% of healthy kernels and 95% of infected kernels are deemed “infected,” reducing the level of infected kernels.

Abstract translation: 通过比较两个波长的反射和透射光来分离镰刀菌感染的谷粒，其中光被健康和感染的核重要地反射和散射，另一个在光被反射和散射的情况下被显着更大程度地被感染 比健康的内核。 具有低内部真空的旋转有孔圆筒的装置允许比较各个内核。 当比较表明内核受到感染时，杠杆将其从气缸中移出，使其能够落入受感染的内核的容器中。 残留在圆筒上的颗粒被刮掉以落入健康的内核的容器中。 虽然结果有所不同，但在一定程度上取决于感染程度，大约90％的健康核和5％感染的核被认为是“健康的”，而大约10％的健康的核和95％的感染的核被认为是“感染的” “降低受感染的内核的水平。

公开(公告)号：US20120148140A1

公开(公告)日：2012-06-14

申请号：US13316294

申请日：2011-12-09

Applicant: Dino Di Carlo ,  Daniel R. Gossett ,  Westbrook M. Weaver

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Westbrook M. Weaver

IPC: G06K9/00

CPC classification number: G01N15/1468 ,  G01N1/31 ,  G01N21/6456 ,  G01N2015/1006 ,  G01N2021/6471 ,  G01N2021/6484 ,  G01N2201/0833

Abstract: A method of monitoring temporal and spatial information of cells includes trapping a plurality of cells within single cell traps contained in a microfluidic device having an inlet, and an outlet. A first fluorescent stain specific to a first target is flowed into the inlet of the device and exposed to the trapped cells. The trapped cells are then imaged as a function of time. A fluorescent stain specific to a different target is flowed into the inlet of the device, the subsequent fluorescent stain having an emission spectrum that substantially overlaps with the emission spectrum of the prior fluorescent stain. The plurality of trapped cells are then imaged again as a function of time. The process can be repeated with additional fluorescent stains having substantially overlapping emission spectra. Images may be subtracted to reveal the contribution of a single fluorescent stain.

Abstract translation: 监测细胞的时间和空间信息的方法包括捕获包含在具有入口和出口的微流体装置中的单细胞捕获器内的多个细胞。 将第一靶标特有的第一荧光染料流入装置的入口并暴露于被捕获的细胞。 被捕获的细胞然后作为时间的函数成像。 特定于不同靶的荧光染料流入器件的入口，随后的荧光染料具有与现有荧光染料的发射光谱基本重叠的发射光谱。 然后，多个捕获的细胞作为时间的函数被再次成像。 可以用具有基本上重叠的发射光谱的附加荧光染料重复该过程。 可以减去图像以显示单个荧光染色剂的贡献。

公开(公告)号：US20120075625A1

公开(公告)日：2012-03-29

申请号：US13213116

申请日：2011-08-19

Applicant: Shintaro TAMURA ,  Masanori Fukawa ,  Ayumu Ishihara ,  Kenichi Shitara ,  Hiroshi Nakajima

Inventor： Shintaro TAMURA ,  Masanori Fukawa ,  Ayumu Ishihara ,  Kenichi Shitara ,  Hiroshi Nakajima

IPC: G01N21/88

CPC classification number: G01N21/00 ,  G01N21/8806 ,  G01N21/9501 ,  G01N21/9506 ,  G01N2201/0833

Abstract: The present invention is to provide an optical surface defect inspection apparatus or an optical surface defect inspection method that can improve a signal-to-noise ratio according to a multi-segmented cell method without performing autofocus operations, and can implement highly sensitive inspection. The present invention is an optical surface defect inspection apparatus or an optical surface defect inspection method in which an inspection beam is applied onto a test subject, an image of a scattered light from the surface of the test subject is formed on a photo-detector, and a defect on the surface of the test subject is inspected based on an output from the photo-detector. The photo-detector has an optical fiber bundle. One end thereof forms a circular light receiving surface to receive the scattered light. The other end thereof is connected to a plurality of light receiving devices. The optical fiber bundle is divided into a plurality of fan-shaped cells in the light receiving surface, and connected to the light emitting devices in units of the cells for performing the inspection based on the outputs of the plurality of cells.

Abstract translation: 本发明提供一种光学表面缺陷检查装置或光学表面缺陷检查方法，其可以在不进行自动对焦操作的情况下，根据多分段单元法提高信噪比，并且可以实现高度敏感的检查。 本发明是一种光学表面缺陷检查装置或光学表面缺陷检查方法，其中将检查光束施加到测试对象上，在光检测器上形成来自受检者表面的散射光的图像， 并且基于光电检测器的输出来检查测试对象的表面上的缺陷。 光检测器具有光纤束。 其一端形成圆形光接收表面以接收散射光。 其另一端连接到多个光接收装置。 光纤束在光接收表面被分成多个扇形单元，并且以基于多个单元的输出执行检查的单元为单位连接到发光器件。

公开(公告)号：US20120057145A1

公开(公告)日：2012-03-08

申请号：US13029992

申请日：2011-02-17

Applicant: James W. Tunnell ,  Brandon Nichols ,  Narasimhan Rajaram ,  Sheldon F. Bish ,  Liang Lim

Inventor： James W. Tunnell ,  Brandon Nichols ,  Narasimhan Rajaram ,  Sheldon F. Bish ,  Liang Lim

IPC: G01J3/42 ,  G01N21/00 ,  G01N21/55 ,  G01J3/44

CPC classification number: G01N21/474 ,  A61B5/0075 ,  A61B5/444 ,  G01J3/42 ,  G01N21/31 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/1736 ,  G01N2021/174 ,  G01N2201/0221 ,  G01N2201/06113 ,  G01N2201/06146 ,  G01N2201/0833

Abstract: Systems comprising an optical fiber switch connected to a light source and an optical fiber probe, the optical fiber probe comprising a first optical fiber connected to the optical fiber switch and a second optical fiber connected to a spectrophotometer. Methods for determining one or more tissue parameters comprising: emitting light from a first optical fiber into a tissue; collecting the light reemitted from the tissue with a second optical fiber; generating a spectra of the light reemitted from the tissue with a spectrophotometer; and utilizing a look-up table based algorithm to determine one or more tissue parameters, wherein the lookup-table based algorithm comprises the steps of: generating a look-up table by measuring the functional form of a reflectance measured by the spectrophotometer using one or more calibration standards with known optical properties; and implementing an iterative fitting routine based on the lookup-table.

Abstract translation: 包括连接到光源的光纤开关和光纤探针的系统，所述光纤探针包括连接到光纤开关的第一光纤和连接到分光光度计的第二光纤。 用于确定一个或多个组织参数的方法，包括：将光从第一光纤发射到组织中; 用第二光纤收集从组织再发射的光; 用分光光度计产生从组织再发射的光的光谱; 以及利用基于查找表的算法来确定一个或多个组织参数，其中所述基于查找表的算法包括以下步骤：通过测量由所述分光光度计测量的反射率的函数形式来生成查找表，使用一个或多个 具有已知光学性能的更多校准标准; 并且基于查找表来实现迭代拟合例程。

公开(公告)号：US20120014835A1

公开(公告)日：2012-01-19

申请号：US13143720

申请日：2010-01-08

Applicant: James Richard Howell ,  Benjamin Masterman Webster ,  Mark Quentin Clark ,  Richard Alfred Howell

Inventor： James Richard Howell ,  Benjamin Masterman Webster ,  Mark Quentin Clark ,  Richard Alfred Howell

IPC: G01N21/76

CPC classification number: B01J19/0046 ,  G01N21/253 ,  G01N21/6452 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: An apparatus for detecting spectra in light emanating from chemical or biochemical reactions occurring in at least one reaction vessel (3) of a plurality of reaction vessels is disclosed. Each reaction vessel (3) has a receptacle portion having an emitting area from which light can emanate. The apparatus may include a masking element (5) having an array of apertures (6) through which light from each reaction vessel (3) can escape. A plurality of light waveguides (7) are arranged to guide light from the apertures (6) in the masking element (5) to a light dispersing device (8) for dispersing the light from each waveguide (7) into a dispersed spectrum. A light detecting device (10) detects specific spectra in the dispersed spectra of light substantially simultaneously. In one embodiment, the apertures (6) are substantially smaller in size than the emitting area of the reaction vessel, but in another embodiment, the apertures (6) are substantially similar in size to the emitting area of the reaction vessel and the light waveguides (7) have a diameter that tapers from a first end substantially similar in diameter to the area of the top of the reaction vessel to a second end that is substantially smaller in diameter. In an alternative embodiment, the light waveguides are not used, when light from the small apertures is directed straight at the light dispersing device. In a further alternative embodiment, several light waveguides are provided for each reaction vessel, each waveguide directing light to a different detector for detecting a different specific spectrum.

Abstract translation: 公开了一种用于检测在多个反应容器（3）中发生的化学或生物化学反应产生的光中的光谱的装置。 每个反应容器（3）具有容纳部分，该容器部分具有能够发出光的发射区域。 该装置可以包括具有孔（6）阵列的掩模元件（5），来自每个反应容器（3）的光可以通过该孔排出。 多个光波导（7）布置成将来自掩模元件（5）中的孔（6）的光引导到用于将来自每个波导（7）的光分散到分散光谱中的光分散装置（8）。 光检测装置（10）基本上同时检测光的分散光谱中的特定光谱。 在一个实施例中，孔（6）的尺寸基本上小于反应容器的发射面积，但是在另一个实施例中，孔（6）的大小与反应容器的发射面积基本相似，并且光波导 （7）的直径从直径基本上类似于反应容器顶部的面积的第一端逐渐变细到直径基本上较小的第二端。 在替代实施例中，当来自小孔的光直接指向光分散装置时，不使用光波导。 在另一替代实施例中，为每个反应容器提供几个光波导，每个波导将光引导到不同的检测器以检测不同的特定光谱。

公开(公告)号：US08098373B2

公开(公告)日：2012-01-17

申请号：US12759082

申请日：2010-04-13

Applicant: Matthew Nelson ,  Patrick Treado ,  Ryan Priore

Inventor： Matthew Nelson ,  Patrick Treado ,  Ryan Priore

IPC: G01J3/28

CPC classification number: G01J3/36 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/027 ,  G01J3/0289 ,  G01J3/0291 ,  G01J3/2823 ,  G01N21/31 ,  G01N2201/0833 ,  G01N2201/0846

Abstract: System and method for spatially and spectrally parallelized FAST. A sample is illuminated to thereby produce interacted photons. The photons are passed through a filter and received at a two-dimensional end of a FAST device wherein said FAST device comprises a two-dimensional array of optical fibers drawn into a one-dimensional fiber stack so as to effectively convert a two-dimensional array of optical fibers into a curvilinear field of view, and wherein said two-dimensional array of optical fibers is configured to receive said photons and transfer said photons out of said fiber array spectral translator device and to a spectrograph through said one-dimensional fiber stack wherein said one-dimensional fiber stack comprises at least two columns of fibers spatially offset in parallel at the entrance slit of said spectrograph. The photons are then detected at a detector to thereby obtain a spectroscopic data set representative of the sample.

Abstract translation: 用于空间和光谱平行化FAST的系统和方法。 照射样品从而产生相互作用的光子。 光子通过过滤器并在FAST装置的二维端接收，其中所述FAST装置包括被拉入一维纤维堆叠的二维光纤阵列，以有效地将二维阵列 的光纤变成曲线视场，并且其中所述二维光纤阵列被配置为接收所述光子并将所述光子从所述光纤阵列光谱转换器装置传送到通过所述一维光纤堆的光谱仪，其中 所述一维纤维堆叠包括在所述光谱仪的入口狭缝处空间上平行偏移的至少两列纤维。 然后在检测器处检测光子，从而获得代表样品的光谱数据集。

公开(公告)号：US20110066014A1

公开(公告)日：2011-03-17

申请号：US12875925

申请日：2010-09-03

Applicant: Kate Leeann Bechtel ,  Tobias Funk ,  Brian Patrick Wilfley ,  Joseph Anthony Heanue

Inventor： Kate Leeann Bechtel ,  Tobias Funk ,  Brian Patrick Wilfley ,  Joseph Anthony Heanue

IPC: A61B5/1455

CPC classification number: A61B5/0059 ,  A61B5/0091 ,  A61B5/14552 ,  A61B5/14553 ,  A61B5/415 ,  A61B5/418 ,  G01J3/02 ,  G01J3/0205 ,  G01J3/0218 ,  G01J3/10 ,  G01J3/2823 ,  G01J3/4406 ,  G01N21/6408 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: The present invention pertains to a method and apparatus for cerebral oximetry. A modulated optical signal based on a digital code sequence is transmitted to the human brain. A temporal transfer characteristic is derived from the modulated optical signal. Oxygen level in the brain is determined based on the temporal transfer characteristic.

Abstract translation: 本发明涉及脑血氧测定法的方法和装置。 基于数字码序列的调制光信号被传送到人脑。 从调制光信号导出时间传递特性。 基于时间转移特征确定脑中的氧水平。

公开(公告)号：US20100032582A1

公开(公告)日：2010-02-11

申请号：US12187815

申请日：2008-08-07

Applicant: Hua Xia ,  Li Zhu ,  Eugene Barash ,  Erin Jean Finehout ,  Matthew Damian Pietrzykowski

Inventor： Hua Xia ,  Li Zhu ,  Eugene Barash ,  Erin Jean Finehout ,  Matthew Damian Pietrzykowski

IPC: G01N21/64

CPC classification number: G01N21/645 ,  B01L3/5027 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/08 ,  G01J3/4406 ,  G01N21/6408 ,  G01N2021/6419 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A fluorescence detection system comprises a light source configured to produce an excitation light, an optical lens and a fiber bundle. The optical lens is configured to focus the excitation light to a sample to emit fluorescence and to collect the fluorescence. The fiber bundle probe comprises a transmitting fiber configured to transmit the excitation light to the optical lens, and a first receiving fiber configured to deliver the collected fluorescence. The fluorescence detection system further comprises a first detector configured to detect the fluorescence delivered by the receiving fiber to generate a response signal, and a processing unit configured to determine information about the samples by analyzing the response signal. Additionally, a fluorescence detection method is also presented.

Abstract translation: 荧光检测系统包括被配置为产生激发光，光学透镜和光纤束的光源。 光学透镜被配置为将激发光聚焦到样品以发射荧光并收集荧光。 纤维束探针包括被配置为将激发光透射到光学透镜的发射光纤和被配置为传送所收集的荧光的第一接收光纤。 荧光检测系统还包括第一检测器，其被配置为检测由接收光纤传送的荧光以产生响应信号;以及处理单元，被配置为通过分析响应信号来确定关于样本的信息。 另外，还提出了荧光检测方法。