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公开(公告)号：WO2012154745A3
公开(公告)日：2013-01-24
申请号：PCT/US2012036949
申请日：2012-05-08
Applicant: ADVANCED LIQUID LOGIC INC , STURMER RYAN , SRINIVASAN VIJAY , SUDARSAN ARJUN
Inventor： STURMER RYAN , SRINIVASAN VIJAY , SUDARSAN ARJUN
IPC: B01J19/00 , G01N1/10 , G01N27/02 , G01N33/483
CPC classification number: B01L3/50273 , B01L3/502792 , B01L2200/0621 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0645 , B01L2300/0816 , B01L2300/0829 , B01L2300/087 , B01L2300/089 , B01L2400/0427 , G01N27/048 , G01R27/16
Abstract: Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.
Abstract translation: 方法包括测量由激发信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。 还公开了存储用于执行该方法的处理器可执行指令的计算机可读介质，以及系统。 系统包括存储在存储器中的处理器，存储器和代码，当执行时，处理器至少要使接收输出电压信号，将激励信号叠加在输出电压信号上，产生叠加信号，将叠加的信号连接到 液滴致动器中的电极，当检测到电极的阻抗时抑制输出电压信号，并测量由激励信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。
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公开(公告)号：WO2012068055A2
公开(公告)日：2012-05-24
申请号：PCT/US2011/060714
申请日：2011-11-15
Applicant: ADVANCED LIQUID LOGIC, INC. , POLLACK, Michael , STURMER, Ryan , PAIK, Philip , PAMULA, Vamsee , SRINIVASAN, Vijay
Inventor： POLLACK, Michael , STURMER, Ryan , PAIK, Philip , PAMULA, Vamsee , SRINIVASAN, Vijay
IPC: G01R27/26
CPC classification number: G01N27/221 , B01L3/502784 , B01L3/502792 , B01L2200/14 , B01L2300/0645 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B41J2/04541 , B41J2/04555 , B41J2/0458 , G01K7/34
Abstract: The invention provides for a method of performing capacitance detection on a droplet actuator. A capacitor may be formed by the combination of a conductive droplet, an insulator layer, and one or more transport electrodes within a droplet actuator. At any given electrode, the capacitance measured is proportional to the footprint area of a droplet thereon. Capacitance detection methods of the invention herein may be used as a real-time verification tool in order to detect the absence, presence, and/or partial presence of a droplet at an electrode; analysis of droplet properties; measurement of droplet size or volume; optimization of the speed of droplet operations; and detection of air bubbles. The method may include applying a base oscillation frequency at the position and detecting a deviation from the base oscillation frequency.
Abstract translation: 本发明提供了一种在液滴致动器上执行电容检测的方法。 可以通过导电液滴，绝缘体层和液滴致动器内的一个或多个输送电极的组合来形成电容器。 在任何给定的电极处，测量的电容与其上的液滴的占地面积成比例。 本发明的电容检测方法可用作实时验证工具，以便检测液滴在电极处的不存在，存在和/或部分存在; 液滴性质分析; 液滴尺寸或体积的测量; 液滴操作速度的优化; 并检测气泡。 该方法可以包括在该位置处施加基本振荡频率并检测与基本振荡频率的偏差。
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公开(公告)号：WO2012037308A2
公开(公告)日：2012-03-22
申请号：PCT/US2011/051691
申请日：2011-09-15
Applicant: ADVANCED LIQUID LOGIC, INC. , FOGLEMAN, Michael , STURMER, Ryan A. , SMITH, Gregory, F.
Inventor： FOGLEMAN, Michael , STURMER, Ryan A. , SMITH, Gregory, F.
IPC: B01J19/00 , G01N27/26 , G01N27/447 , C12Q1/68
CPC classification number: B81B7/02 , B01L3/502792 , B01L2200/0605 , B01L2200/143 , B01L2300/0645 , B01L2300/0816 , B01L2300/089 , B01L2400/0424 , B01L2400/0427 , C12Q1/6825 , G01F22/00 , G01N35/10 , C12Q2527/107 , C12Q2563/159 , C12Q2565/629
Abstract: The present invention is directed to droplet actuator systems, devices, and methods. In one embodiment, a microfluidic article of manufacture is provided. The microfluidic article of manufacture includes a first substrate; a second substrate separated from the first substrate to form a droplet operations gap; gap height setting spacers associated with the first and/or second substrate or situated between the first and second substrates; a spring forcing the second substrate against the gap height setting spacers, thereby establishing a substantially uniform gap height between the first and second substrates; and electrodes associated with the first and/or second substrate and configured to conduct droplet operations in the droplet operations gap.
Abstract translation: 本发明涉及液滴致动器系统，装置和方法。 在一个实施例中，提供了微流体制品。 微流体制品包括第一基底; 与所述第一衬底分离以形成液滴操作间隙的第二衬底; 与第一和/或第二衬底相关联或位于第一和第二衬底之间的间隙高度设置间隔件; 弹簧迫使第二基板抵靠间隙高度设定间隔件，由此在第一和第二基板之间建立基本均匀的间隙高度; 以及与第一和/或第二衬底相关联并且被配置成在液滴操作间隙中传导液滴操作的电极。
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24.发明申请
公开(公告)号：WO2012009320A2
公开(公告)日：2012-01-19
申请号：PCT/US2011043650
申请日：2011-07-12
Applicant: ADVANCED LIQUID LOGIC INC , SRINIVASAN VIJAY , BAUER WILLIAM CRAIG , SMITH GREGORY F , STURMER RYAN A , HUA ZHISHAN , ECKHARDT ALLEN E
Inventor： SRINIVASAN VIJAY , BAUER WILLIAM CRAIG
CPC classification number: C12M1/42 , B01L3/502792 , B01L2200/0647 , B01L2300/0672 , B01L2400/0427 , B01L2400/0439 , B01L2400/088 , C12M47/06 , C12N1/066 , C12N13/00
Abstract: The invention relates to a droplet actuator for conducting droplet operations. The actuator includes a bottom substrate and a top substrate separated from the bottom substrate to form a gap. An arrangement of droplet operations electrodes may be located on a surface of the bottom substrate and/or top substrate. Optionally, a sample reservoir may hold a quantity of a sample fluid containing cells. A disruption device which can take various forms is used to lyse the cells in the sample or in a sample droplet to thereby conduct operations on samples having lysed cells therein.
Abstract translation: 本发明涉及一种用于进行液滴操作的液滴致动器。 致动器包括底部基板和与底部基板分离以形成间隙的顶部基板。 液滴操作电极的布置可以位于底部基板和/或顶部基板的表面上。 任选地，样品储存器可以容纳一定量的含有细胞的样品流体。 可以采用各种形式的破坏装置来裂解样品或样品液滴中的细胞，从而对其中具有裂解细胞的样品进行操作。
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公开(公告)号：WO2011002957A3
公开(公告)日：2011-08-04
申请号：PCT/US2010040705
申请日：2010-07-01
Applicant: ADVANCED LIQUID LOGIC INC , POLLACK MICHAEL G , SRINIVASAN VIJAY , PAMULA VAMSEE K , BRACKETT GEORGE , BRAFFORD KEITH R , CLARK DARIUS , HUA ZHISHAN , NORTON SCOTT , SISTA RAMAKRISHNA , SMITH GREGORY F , STURMER RYAN A , SUDARSAN ARJUN , THWAR PRASANNA , WANG TIH-HONG , WINGER THEODORE
Inventor： POLLACK MICHAEL G , SRINIVASAN VIJAY , PAMULA VAMSEE K , BRACKETT GEORGE , BRAFFORD KEITH R , CLARK DARIUS , HUA ZHISHAN , NORTON SCOTT , SISTA RAMAKRISHNA , SMITH GREGORY F , STURMER RYAN A , SUDARSAN ARJUN , THWAR PRASANNA , WANG TIH-HONG , WINGER THEODORE
CPC classification number: B01L3/502707 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/5025 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502784 , B01L3/502792 , B01L2200/0673 , B01L2200/0689 , B01L2300/0819 , B01L2300/0861 , B01L2300/087 , B01L2300/0874 , B01L2300/088 , B01L2300/0883 , B01L2400/0415 , B01L2400/0427 , B01L2400/0442 , B01L2400/0457 , B01L2400/0469 , B01L2400/0622 , B01L2400/0688 , B01L2400/088
Abstract: The invention provides droplet actuator devices for facilitating certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuators that facilitate droplet operations in three dimensions. In another embodiment, the invention provides for cost-effective production of droplet actuators. In another embodiment, the invention provides for replacing one or more components of a droplet actuator. In another embodiment, the invention provides droplet actuators using printed conductive inks to form electrodes and/or ground planes. In another embodiment, the invention provides a magnetic clamping fixture for assembling droplet actuators. In another embodiment, the invention provides simple, low cost power sources for use in combination with microfluidic systems. In another embodiment, the invention provides an immunoassay multiplexing platform that uses digital microfluidics and real-time imaging of flash-based chemiluminescent signals. In still another embodiment, the invention provides droplet actuator devices that are fabricated using a plasma treatment process to raise substrate surface energy.
Abstract translation: 本发明提供了用于促进某些液滴致动分子技术的液滴致动器装置。 在一个实施例中，本发明提供了促进三维液滴操作的液滴致动器。 在另一个实施例中，本发明提供了液滴致动器的成本有效的生产。 在另一个实施例中，本发明提供了替换液滴致动器的一个或多个部件。 在另一个实施例中，本发明提供使用印刷导电油墨形成电极和/或接地平面的液滴致动器。 在另一个实施例中，本发明提供了一种用于组装液滴致动器的磁性夹具。 在另一个实施例中，本发明提供了与微流体系统结合使用的简单，低成本的电源。 在另一个实施方案中，本发明提供了使用数字微流体和基于闪光的化学发光信号的实时成像的免疫测定多路复用平台。 在又一个实施例中，本发明提供了使用等离子体处理工艺制造以提高衬底表面能量的液滴致动器装置。
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Patent Agency Ranking
公开(公告)号：WO2011020011A3
公开(公告)日：2011-06-16
申请号：PCT/US2010045461
申请日：2010-08-13
Applicant: ADVANCED LIQUID LOGIC INC , POLLACK MICHAEL G , SRINIVASAN VIJAY , ECKHARDT ALLEN E , THWAR PRASANNA , HUA ZHISHAN , SUDARSAN ARJUN , WINGER THEODORE
Inventor： POLLACK MICHAEL G , SRINIVASAN VIJAY , ECKHARDT ALLEN E , THWAR PRASANNA , HUA ZHISHAN , SUDARSAN ARJUN , WINGER THEODORE
CPC classification number: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L2200/0668 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/686 , G01N33/54326 , G01N2035/00425 , G01N2035/1058 , G01N2035/1062 , C12Q2549/101 , C12Q2527/15 , C12Q2527/107 , C12Q2561/113
Abstract: The invention is directed to certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuator methods for detection of single nucleotide polymorphisms (SNPs) in a DNA sequence using digital microfluidics, including droplet actuator-based sample preparation and SNP analysis. In another embodiment, the invention provides droplet actuator devices and methods for providing integrated sample preparation and multiplexed detection of an infectious agent, such as HIV. In yet another embodiment, the invention provides droplet actuator devices and techniques for PCR amplification and detection of specific nucleic acid sequences using digital microfluidics, including droplet actuator-based sample preparation and target nucleic acid analysis. In yet another embodiment the invention provides methods for performing hot-start PCR on a droplet actuator. In yet another embodiment, the method of the invention combines PCR amplification with pyrosequencing to investigate specific sequences.
Abstract translation: 本发明涉及某些液滴致动分子技术。 在一个实施方案中，本发明提供用于使用数字微流体检测DNA序列中的单核苷酸多态性（SNP）的液滴致动器方法，包括基于液滴致动器的样品制备和SNP分析。 在另一个实施方案中，本发明提供了液滴致动器装置和用于提供综合样品制备和多重检测感染因子如HIV的方法。 在另一个实施方案中，本发明提供了使用数字微流体（包括基于液滴致动器的样品制备和靶核酸分析）的PCR扩增和检测特异性核酸序列的液滴致动器装置和技术。 在另一个实施方案中，本发明提供了在液滴致动器上进行热启动PCR的方法。 在另一个实施方案中，本发明的方法将PCR扩增与焦磷酸测序结合以研究特异性序列。
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公开(公告)号：WO2010006166A3
公开(公告)日：2010-04-29
申请号：PCT/US2009050101
申请日：2009-07-09
Applicant: ADVANCED LIQUID LOGIC INC , PAMULA VAMSEE , THWAR PRASANNA , SUDARSAN ARJUN , SISTA RAMAKRISHNA , SULLIVAN MICHAEL , NORTON SCOTT
Inventor： PAMULA VAMSEE , THWAR PRASANNA , SUDARSAN ARJUN , SISTA RAMAKRISHNA , SULLIVAN MICHAEL , NORTON SCOTT
IPC: G01N33/543 , G01N27/00
Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode -mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
Abstract translation: 本发明提供了在液滴中重新分布磁响应珠的方法。 该方法可以包括提供包括磁响应珠的液滴。 可以在具有足够强度的磁场的区域内提供液滴，以将磁响应珠吸引到液滴的边缘或朝向液滴的边缘，或以其他方式使液滴区域化或聚集在液滴内。 该方法还可以包括使用液滴在液滴操作表面上进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 液滴操作在某些情况下可能是电极介导的。 液滴操作可以重新分配和/或循环液滴内的磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括目标分析物。 磁响应珠粒的重新分布可以使目标分析物结合到磁响应珠粒上。 在一些情况下，液滴可以包含洗涤缓冲液中的未结合物质。 磁响应珠粒的重新分布导致未结合的物质从磁性响应珠粒的聚集组或亚组的空隙释放。
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公开(公告)号：WO2008124846A3
公开(公告)日：2009-12-23
申请号：PCT/US2008059955
申请日：2008-04-10
Applicant: ADVANCED LIQUID LOGIC INC , POLLACK MICHAEL , PAMULA VAMSEE , SRINIVASAN VIJAY
Inventor： POLLACK MICHAEL , PAMULA VAMSEE , SRINIVASAN VIJAY
CPC classification number: B01L3/502761 , B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/16 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/088 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , G01N27/447
Abstract: The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.
Abstract translation: 本发明提供了用于在液滴致动器中分配液滴的结构的方法和方法的非限制性实例。 本发明的液滴致动器结构和方法与现有技术的液滴致动器相比表现出许多优点。 在各种实施例中，与现有的液滴致动器相比，本发明的结构和方法尤其提供了改进的效率，吞吐量，可扩展性和/或液滴均匀性。 此外，在一些实施例中，液滴致动器提供用于加载和/或卸载流体和/或液滴的改进方法的构造。 在其他实施例中，液滴致动器提供用于以基本上同时和/或基本顺序的方式装载多个流体储存器的流体加载构造。
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公开(公告)号：WO2009140373A3
公开(公告)日：2009-11-19
申请号：PCT/US2009/043774
申请日：2009-05-13
Applicant: ADVANCED LIQUID LOGIC, INC. , POLLACK, Michael , HUA, Zhishan , ECKHARDT, Allen , THWAR, Prasanna , SRINIVASAN, Vijay , PAMULA, Vamsee
Inventor： POLLACK, Michael , HUA, Zhishan , ECKHARDT, Allen , THWAR, Prasanna , SRINIVASAN, Vijay , PAMULA, Vamsee
Abstract: The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
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公开(公告)号：WO2009135205A2
公开(公告)日：2009-11-05
申请号：PCT/US2009042699
申请日：2009-05-04
Applicant: ADVANCED LIQUID LOGIC INC , CHILDRENS MEDICAL CENTER , PAMULA VAMSEE K , EMANI SITARAM , ECKHARDT ALLEN
Inventor： PAMULA VAMSEE K , EMANI SITARAM , ECKHARDT ALLEN
CPC classification number: G01N33/86 , B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L7/52 , B01L2200/0605 , B01L2200/0668 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0406 , B01L2400/0409 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/046 , B01L2400/0487 , G01N33/5302
Abstract: The invention provides techniques for coagulating blood on a droplet actuator. The invention also provides methods of manipulating the coagulated blood including a variety of droplet operations that may be conducted using the coagulated blood. Further, the invention provides a variety of assays that make use of the coagulated blood or various blood samples as input.
Abstract translation: 本发明提供了用于在液滴致动器上凝结血液的技术。 本发明还提供了操作凝血血液的方法，包括可以使用凝血血液进行的各种液滴操作。 此外，本发明提供使用凝血血液或各种血液样品作为输入的各种测定法。
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