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    Fused cell line and method of obtaining the same
    22.
    发明公开
    Fused cell line and method of obtaining the same 失效
    融合的细胞系，以及它们的制备方法

    公开(公告)号：EP0911395A3

    公开(公告)日：2003-09-10

    申请号：EP98103298.0

    申请日：1998-02-25

    Applicant: National Agricultural Research Organization ,  Bio-oriented Technology Research Advancement Institution

    Inventor： TSUji. Kenkou ,  Osada, Kazuhiro ,  Yamamoto, Mari ,  Kawahara, Hiroharu

    IPC: C12N15/06

    CPC classification number: C12N5/163 ,  C12N2500/25 ,  C12N2503/02

    Abstract: Provided are a human immunocompetent fused cell line, characterized in that the mutant cell line ICLU-B derived from the human Burkitt lymphoma cell line Raji, ICLU-T derived from human T-cell lymphocytic leukemia cell line PEER, or ICLU-E derived from the human eosinophilic leukemia cell line EoL-1 is used as a parental cell line, wherein said mutant cell line is obtained by selecting an 8-azaguanine- or 6-thioguanine-resistant clone from said Burkitt lymphoma, said T-cell lymphocytic leukemia and said eosinophilic leukemia cell line, respectively, and said clone is treated in order to render it capable of growing in serum-free medium, and a method of obtaining said human immunocompetent fused cell line. The human immunocompetent fused cell line obtained by the present invention can be used to investigate food functions, novel drugs and the production thereof with the view to the elucidation of mechanisms of phenomena of the immune system such as allergies, cancers and the like, and the prevention, diagnosis and therapy of such diseases.

    Fused cell line and method of obtaining the same
    25.
    发明公开
    Fused cell line and method of obtaining the same 失效
    Fusioniertes Zellinie und Verfahren zu deren Herstellung

    公开(公告)号：EP0911395A2

    公开(公告)日：1999-04-28

    申请号：EP98103298.0

    申请日：1998-02-25

    Applicant: Director-General of National Research Institute of Vegetables Ornamental Plants and Tea, Ministry of Agriculture, Forestry and ,  Bio-oriented Technology Research Advancement Institution

    Inventor： TSUji. Kenkou ,  Osada, Kazuhiro ,  Yamamoto, Mari ,  Kawahara, Hiroharu

    IPC: C12N15/06

    CPC classification number: C12N5/163 ,  C12N2500/25 ,  C12N2503/02

    Abstract: Provided are a human immunocompetent fused cell line, characterized in that the mutant cell line ICLU-B derived from the human Burkitt lymphoma cell line Raji, ICLU-T derived from human T-cell lymphocytic leukemia cell line PEER, or ICLU-E derived from the human eosinophilic leukemia cell line EoL-1 is used as a parental cell line, wherein said mutant cell line is obtained by selecting an 8-azaguanine- or 6-thioguanine-resistant clone from said Burkitt lymphoma, said T-cell lymphocytic leukemia and said eosinophilic leukemia cell line, respectively, and said clone is treated in order to render it capable of growing in serum-free medium, and a method of obtaining said human immunocompetent fused cell line. The human immunocompetent fused cell line obtained by the present invention can be used to investigate food functions, novel drugs and the production thereof with the view to the elucidation of mechanisms of phenomena of the immune system such as allergies, cancers and the like, and the prevention, diagnosis and therapy of such diseases.

    Abstract translation: 本发明提供一种人免疫活性融合细胞系，其特征在于衍生自人伯基特淋巴瘤细胞系Raji的突变细胞系ICLU-B，源自人T细胞淋巴细胞白血病细胞系PEER的ICLU-T或源自 人嗜酸粒细胞白血病细胞系EoL-1用作亲本细胞系，其中所述突变细胞系通过从所述伯基特淋巴瘤，所述T细胞淋巴细胞性白血病和所述T细胞淋巴细胞白血病中选择8-氮鸟嘌呤或6-硫鸟嘌呤抗性克隆 分别表示嗜酸粒细胞白血病细胞系，并且对所述克隆进行处理以使其能够在无血清培养基中生长，以及获得所述人免疫活性融合细胞系的方法。 通过本发明获得的人免疫活性融合细胞系可以用于研究食物功能，新药和其生产，以便阐明免疫系统现象的机制，例如过敏，癌症等，以及 预防，诊断和治疗这些疾病。

    GENES PARTICIPATING IN THE SYNTHESIS OF FATTY ACID HAVING TRANS-11-,CIS-13- CONDJUGATED DOUBLE BOND AND UTILIZATION THEREOF
    26.
    发明公开
    GENES PARTICIPATING IN THE SYNTHESIS OF FATTY ACID HAVING TRANS-11-,CIS-13- CONDJUGATED DOUBLE BOND AND UTILIZATION THEREOF 审中-公开
    ON脂肪酸与反式11，顺式13的共轭双键的相关基因及其用途的合成

    公开(公告)号：EP1437412A1

    公开(公告)日：2004-07-14

    申请号：EP02765609.9

    申请日：2002-09-20

    Applicant: Plantech Research Institute ,  Bio-oriented Technology Research Advancement Institution

    Inventor： OSUMI, Mari, Plantech Research Institute ,  MURASE, Junko, Plantech Research Institute ,  IMAMURA, Jun, Plantech Research Institute

    IPC: C12N15/54 ,  C12N9/10 ,  C12N5/14 ,  C12P7/64 ,  A23D9/00 ,  A23L1/29 ,  C07C57/03 ,  A01H5/00 ,  A23K1/16

    CPC classification number: C12N9/0083 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C07K14/415 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  A23V2250/1866

    Abstract: An object of the present invention is to clone a gene which is involved in synthesis of fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bone at position Δ12. The present invention provides a gene having any one of the following nucleotide sequences:

    (A) a nucleotide sequence encoding an amino acid sequence shown in SEQ ID NO: 1 or 12;
    (B) a nucleotide sequence encoding an amino acid sequence comprising a deletion, addition or substitution of one or several amino acids with respect to the amino acid sequence shown in SEQ NO: 1 or 12, and having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12;
    (C) a nucleotide sequence shown in SEQ ID NO: 2 or 13;
    (D) a nucleotide sequence comprising a deletion, addition or substitution of one or several nucleotides with respect to the nucleotide sequence shown in SEQ ID NO: 2 or 13, and encoding a protein having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12; and
    (E) a nucleotide sequence hybridizing with the nucleotide sequence shown in SEQ ID NO: 2 or 13 or a complementary sequence thereof under stringent conditions, and encoding a protein having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12.

    Abstract translation: 基因包含E.G. 的碱基序列的编码在（I）的氨基酸序列或（XII）用387个或395个氨基酸，分别的碱基序列（II）或（XIII）与1287或1374个碱基对，分别或它们的衍生物的存在或 编码能够合成具有在增量从脂肪酸与双键的反式-11-顺式-13共轭双键的12位置的脂肪酸组成的蛋白质，是新的。 所有序列在说明书中给出。 因此独立权利要求中包括了：（1）一种蛋白质含有基于（I）的序列（I）或（XII），或在氨基酸序列或（XII）的氨基酸序列，但是具有缺失一些氨基酸， 添加或substituiertem，并且能够合成具有选自脂肪酸在Δ-12位置上的反式-11-顺式-13共轭双键与双键的脂肪酸的; （2）含有的新基因的载体; （3）的转化的宿主细胞含有的（2）的新的基因或载体; （4）制造携带有通过使用任何的蛋白质的从脂肪酸转化与双键在δ-12位置上的反式-11-顺式-13共轭双键的脂肪酸，或通过培养转化的宿主细胞; （5）提高生产的脂肪酸与脂肪酸与双键的反式-11-顺式-13共轭双键中的在宿主细胞中增量12位被与该基因或载体转化宿主，则选择 与增强在生产脂肪酸的转化的宿主细胞; （6）从与（III）的碱基序列的引物的组合制成的引物组或（IV）与31或26个碱基对，分别与用（V）与23个碱基对的碱基序列的引物; 或从与（VI）的碱基序列和（VII）与22和23个碱基对，分别引物; 或从与（XIV）与30或26个碱基对，分别与的（XVI）的碱基序列的23个碱基对的碱基序列或（XV）的引物; 或从与（XVII）的碱基序列或（XIX）与25或22个碱基对，分别与用（XVIII）或（XX）的27或25个碱基对，的碱基序列的引物的引物分别所有 在说明书中给出的; （7）从所述的转化植物细胞产生的种子（3）; 衍生物（8）提高生产脂肪酸携带反式-11-顺式-13共轭双键和/或它在由宿主细胞：（a）用已经定义的蛋白质编码的基因的宿主细胞; （B）生长的转化，以使基因的表达; 和（c）选择具有增强的脂肪酸和/或它的衍生物的生产的宿主细胞; （9）从种子产生的种子油（7）; （10）生产的种子油，其包括：（a）用指定的蛋白编码基因的植物细胞; （B）生长的转化成能育植物体; （c）获得基于在生产脂肪酸携带反式-11-顺式-13共轭双键和/或它的供选择衍生物的增强从肥沃规划体下一代的种子; 和（d）从生产检查种子油和/或脂肪; 由（10）的方法产生（11）种子油; （12）功能性健康食品含有种子或它们的处理物。 和（13）动物饲料含有种子或它们的加工材料。 活动：抑制细胞; 厌食; 抗糖尿病。 没有生物学数据中给出。 作用机理：没有给出。

    BZIP TRANSCRIPITON FACTORS THAT CONTROLS EXPRESSION OF THE STORAGE PROTEIN IN THE RICE PLANT
    28.
    发明公开
    BZIP TRANSCRIPITON FACTORS THAT CONTROLS EXPRESSION OF THE STORAGE PROTEIN IN THE RICE PLANT 审中-公开
    DIE EXPRESSION DES SPEICHERPROTEINS IN DER REISPFLANZE KONTROLLIERENDE BZIP-TRANSKRIPTIONSFAKTOREN

    公开(公告)号：EP1327685A1

    公开(公告)日：2003-07-16

    申请号：EP01976664.1

    申请日：2001-10-11

    Applicant: National Institute of Agrobiological Sciences ,  Bio-oriented Technology Research Advancement Institution

    Inventor： TAKAIWA, Fumio ,  ONODERA, Yasuyuki

    IPC: C12N15/29 ,  C12N5/14 ,  C07K14/415 ,  A01H5/00

    CPC classification number: C12N15/8251 ,  C07K14/415 ,  C12N15/8217 ,  C12N15/8234 ,  C12N15/8238 ,  C12N15/8261 ,  Y02A40/146

    Abstract: cDNAs (RISBZ1, RISBZ4, and RISBZ5) encoding bZIP transcription factors were isolated from a cDNA library originating in rice plant seed. The cDNAs encode novel proteins and have binding activity to the GCN4 motif. Among them, RISBZ1 activated transcription mediated by the GCN4 motif by 100-fold or more. Since the expression of RISBZ1 precedes the expression of a seed storage protein gene and is expressed only in maturing seeds, it is suggested that RISBZ1 controls the expression of rice seed storage proteins. In addition, by linking the recognition sequence of the transcription factor, the GCN4 motif, in tandem and introducing it into the promoter for a gene encoding seed storage protein to facilitate its binding to the transcription factor RISBZ1, expression of a foreign gene under the control of the modified promoters is greatly enhanced.

    Abstract translation: 从源于水稻种子的cDNA文库中分离出编码bZIP转录因子的cDNA（RISBZ1，RISBZ4和RISBZ5）。 cDNAs编码新的蛋白质并具有与GCN4基序的结合活性。 其中，RISBZ1通过GCN4基序介导的转录活化100倍以上。 由于RISBZ1的表达先于种子储存蛋白基因的表达，仅在成熟的种子中表达，所以建议RISBZ1控制水稻种子储存蛋白的表达。 此外，通过将转录因子的识别序列GCN4基序串联连接并将其引入编码种子储存蛋白的基因的启动子，以促进其与转录因子RISBZ1的结合，在控制下的外源基因的表达 的改性启动子大大增强。

    Methods to evaluate rice palatability and to select rice with platable traits through analysis of half grain of unhulled/unpulische rice
    30.
    发明公开
    Methods to evaluate rice palatability and to select rice with platable traits through analysis of half grain of unhulled/unpulische rice 有权
    Verfahren zurAbschätzungder Schmackhaftigkeit des Reises und zur Auswahl eines schmackhaften Reises。

    公开(公告)号：EP1298222A2

    公开(公告)日：2003-04-02

    申请号：EP02019424.7

    申请日：2002-08-30

    Applicant: National Food Research Institute ,  Bio-oriented Technology Research Advancement Institution

    Inventor： Ohtsubo, Kenichi ,  Okadome, Hiroshi ,  Nakamura, Sumiko ,  Haraguchi, Kazutomo ,  Yoza, Kouichi ,  Okunishi, Tomoya ,  Suzuki, Keitaro

    IPC: C12Q1/68

    CPC classification number: C12Q1/6895 ,  C12Q2525/179 ,  C12Q2600/156

    Abstract: Provided is a DNA-level rice palatability evaluation method of using a very small quantity of rice, especially a half or one grain of rice as a sample. This is to evaluate the palatability of rice, not disrupting the embryo of a rice grain that is to be the origin of a rice plant of the coming generation, and to select palatable rice. The method comprises amplifying the DNA extracted from a rice plant, unhulled rice, unpolished rice, polished rice, boiled rice, rice cake or ground powder thereof through PCR in the presence of an STS primer or a random primer, selecting DNA bands of close correlation with palatability evaluation from the resulting amplified DNA bands, and using it as a DNA marker for palatability evaluation to select palatable rice.

    Abstract translation: 提供了使用非常少量的米，特别是一米或一米的米作为样品的DNA级水稻可口性评价方法。 这是为了评估大米的适口性，不会破坏作为下一代水稻植物来源的米粒的胚胎，并选择可口的米饭。 该方法包括在STS引物或随机引物的存在下，通过PCR扩增从水稻植物提取的DNA，未脱水的米，未糙米，抛光米，煮米，米饼或其研磨粉末，选择密切相关的DNA条带 从得到的扩增的DNA条带进行适口性评价，并将其用作适口性评价的DNA标记来选择可口的水稻。

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