公开(公告)号：WO2006104899A3

公开(公告)日：2007-01-25

申请号：PCT/US2006010821

申请日：2006-03-24

Applicant: MASSACHUSETTS INST TECHNOLOGY ,  POPESCU GABRIEL ,  DASARI RAMACHANDRA ,  FELD MICHAEL ,  IKEDA TAKAHIRO

Inventor： POPESCU GABRIEL ,  DASARI RAMACHANDRA ,  FELD MICHAEL ,  IKEDA TAKAHIRO

IPC: G01N21/45

CPC classification number: H01L27/14601 ,  G01N15/10 ,  G01N15/1475 ,  G01N21/453 ,  G01N33/49 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/145 ,  G01N2015/1454 ,  G01N2015/1497 ,  G06K9/00134

Abstract: Hubert phase microscopy (HPM) as an optical technique for measuring high transverse resolution quantitative phase images associated with optically transparent objects. Due to its single-shot nature, HPM is suitable for investigating rapid phenomena that take place in transparent structures such as biological cells. A preferred embodiment is used for measuring biological systems including measurements on red blood cells, while its ability to quantify dynamic processes on the millisecond scale, for example, can be illustrated with measurements on evaporating micron- size water droplets.

Abstract translation: Hubert相位显微镜（HPM）作为用于测量与光学透明物体相关的高横向分辨率定量相位图像的光学技术。 由于其单次性质，HPM适用于调查在诸如生物细胞的透明结构中发生的快速现象。 优选实施例用于测量生物系统，包括对红细胞的测量，而其例如在毫秒级上量化动态过程的能力可以用蒸发微米级水滴的测量来说明。

公开(公告)号：WO2005001445A2

公开(公告)日：2005-01-06

申请号：PCT/US2004/019456

申请日：2004-06-18

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  FANG-YEN, Christopher, M. ,  POPESCU, Gabriel ,  YANG, Changhuei ,  WAX, Adam, P. ,  DASARI, Ramachandra, R. ,  FELD, Michael, S.

Inventor： FANG-YEN, Christopher, M. ,  POPESCU, Gabriel ,  YANG, Changhuei ,  WAX, Adam, P. ,  DASARI, Ramachandra, R. ,  FELD, Michael, S.

IPC: G01N21/45

CPC classification number: G01B9/02091 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/7232 ,  G01B9/02002 ,  G01B9/02007 ,  G01B9/02011 ,  G01B9/02057 ,  G01B9/02063 ,  G01B9/02067 ,  G01B9/02069 ,  G01B9/02071 ,  G01B9/02072 ,  G01B9/02078 ,  G01B9/02083 ,  G01B9/0209 ,  G01B2290/45 ,  G01B2290/60 ,  G01B2290/70 ,  G01J9/04 ,  G01N21/45

Abstract: Preferred embodiments of the present invention are directed to systems for phase measurement which address the problem of phase noise using combinations of a number of strategies including, but not limited to, common-path interferometry, phase referencing, active stabilization and differential measurement. Embodiment are directed to optical devices for imaging small biological objects with light. These embodiments can be applied to the fields of, for example, cellular physiology and neuroscience. These preferred embodiments are based on principles of phase measurements and imaging technologies. The scientific motivation for using phase measurements and imaging technologies is derived from, for example, cellular biology at the sub-micron level which can include, without limitation, imaging origins of dysplasia, cellular communication, neuronal transmission and implementation of the genetic code. The structure and dynamics of sub-cellular constituents cannot be currently studied in their native state using the existing methods and technologies including, for example, x-ray and neutron scattering. In contrast, light based techniques with nanometer resolution enable the cellular machinery to be studied in its native state. Thus, preferred embodiments of the present invention include systems based on principles of interferometry and/or phase measurements and are used to study cellular physiology. These systems include principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) wherein interference within the cellular components themselves is used, or in the alternative the principles of LCI and LSS can be combined to result in systems of the present invention

Abstract translation: 本发明的优选实施例针对用于相位测量的系统，其使用多种策略的组合来解决相位噪声的问题，所述策略包括但不限于共路干涉测量，相位参考， 主动稳定和差分测量。 实施例针对用于利用光对小生物物体成像的光学装置。 这些实施例可以应用于例如细胞生理学和神经科学领域。 这些优选实施例基于相位测量和成像技术的原理。 使用相位测量和成像技术的科学动机源于例如亚微米级的细胞生物学，其可以包括但不限于发育异常的成像起源，细胞通信，神经元传递和遗传密码的实施。 亚细胞组分的结构和动力学目前不能用其原生状态使用包括例如X射线和中子散射在内的现有方法和技术进行研究。 相比之下，具有纳米分辨率的基于光的技术使得细胞机器能够以其原生状态进行研究。 因此，本发明的优选实施例包括基于干涉测量和/或相位测量原理的系统，并用于研究细胞生理学。 这些系统包括使用光学干涉仪测量相位的低相干干涉测量法（LCI）或其中使用蜂窝部件本身内的干扰的光散射光谱学（LSS）的原理，或者替代地，可以组合LCI和LSS的原理 在本发明的系统中