公开(公告)号：WO2007140195A3

公开(公告)日：2007-12-06

申请号：PCT/US2007/069513

申请日：2007-05-23

Applicant: CARGILL, INCORPORATED ,  HILBERT, Brent, H. ,  SOLHEID, Christopher ,  MCFARLAN, Sara, C. ,  KHARE, Anil, B. ,  DESOUZA, Mervyn ,  SANCHEZ-RIERA, Fernando, A. ,  FLOY, Kent ,  HICKS, Paula, M.

Inventor： HILBERT, Brent, H. ,  SOLHEID, Christopher ,  MCFARLAN, Sara, C. ,  KHARE, Anil, B. ,  DESOUZA, Mervyn ,  SANCHEZ-RIERA, Fernando, A. ,  FLOY, Kent ,  HICKS, Paula, M.

IPC: C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

Abstract: Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

公开(公告)号：WO2007103389A2

公开(公告)日：2007-09-13

申请号：PCT/US2007/005718

申请日：2007-03-07

Applicant: CARGILL, INCORPORATED ,  BURKE, Ellen ,  GORT, Steven, John ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  MCFARLAN, Sara, C. ,  RICHARDSON, Toby ,  SOLHEID, Christopher ,  WEINER, David ,  ZHAO, Lishan

Inventor： BURKE, Ellen ,  GORT, Steven, John ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  MCFARLAN, Sara, C. ,  RICHARDSON, Toby ,  SOLHEID, Christopher ,  WEINER, David ,  ZHAO, Lishan

IPC: C12P17/00 ,  C07D209/20 ,  C12N9/88 ,  A23L1/22

CPC classification number: C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R3R and S5R configurations, and salts thereof.

Abstract translation: 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性（包括热稳定性和耐热活性），以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种制药，农业和工业环境中。 在一些实施方案中，本发明提供了用于生产R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体诸如R的多肽和生物合成途径 ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，如R3R和S5R构型及其盐。

公开(公告)号：WO2006113897A2

公开(公告)日：2006-10-26

申请号：PCT/US2006/014968

申请日：2006-04-20

Applicant: CARGILL, INCORPORATED ,  LAPLAZA, Jose ,  ANDERSON, James, C. ,  DESOUZA, Mervyn, L. ,  HICKS, Paula, M. ,  KOLLMANN, Sherry, R.

Inventor： LAPLAZA, Jose ,  ANDERSON, James, C. ,  DESOUZA, Mervyn, L. ,  HICKS, Paula, M. ,  KOLLMANN, Sherry, R.

IPC: A61K31/7048

CPC classification number: C12P17/10 ,  A61K31/7048 ,  Y02A50/473

Abstract: Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.

Abstract translation: 提供了莫纳甜甜味剂体内生产的产品和方法。 产品包括经遗传修饰以分泌或改善莫纳甜分泌的微生物; 经遗传修饰以产生莫纳甜的微生物; 和经遗传修饰以分泌或改善莫纳甜分泌并产生莫纳甜的微生物。 这些方法包括在这种基因工程微生物中产生莫纳甜。

公开(公告)号：WO2005020721A1

公开(公告)日：2005-03-10

申请号：PCT/US2004/027454

申请日：2004-08-25

Applicant: CARGILL INC. ,  HICKS, Paula, M.

Inventor： HICKS, Paula, M.

IPC: A23L2/60

CPC classification number: A23L2/60 ,  A23L27/31

Abstract: The present invention relates to novel beverage compositions comprising monatin and methods for making such compositions. The present invention also relates to beverage compositions comprising specific monatin stereoisomers, specific blends of monatin stereoisomers, and/or monatin produced via a biosynthetic pathway in vivo (e.g., inside cells) or in vitro.

Abstract translation: 本发明涉及包含莫纳甜的新型饮料组合物和制备这种组合物的方法。 本发明还涉及包含特定莫纳甜立体异构体，莫纳甜立体异构体的特异性共混物和/或通过体内生物合成途径（例如细胞内）或体外产生的莫纳甜的饮料组合物。

公开(公告)号：WO2011153378A1

公开(公告)日：2011-12-08

申请号：PCT/US2011/038967

申请日：2011-06-02

Applicant: ABUNDA NUTRITION, INC. ,  KISHORE, Ganesh M. ,  MOTION, Michael ,  HICKS, Paula M. ,  HANSEN, Jorgen ,  HOUGHTON-LARSEN, Jens ,  HANSEN, Esben Halkjaer ,  MIKKELSEN, Michael Dalgaard ,  TAVARES, Sabina ,  BLOM, Charlotte

Inventor： KISHORE, Ganesh M. ,  MOTION, Michael ,  HICKS, Paula M. ,  HANSEN, Jorgen ,  HOUGHTON-LARSEN, Jens ,  HANSEN, Esben Halkjaer ,  MIKKELSEN, Michael Dalgaard ,  TAVARES, Sabina ,  BLOM, Charlotte

IPC: A01H5/00 ,  C12N5/04 ,  C12P7/58 ,  C07H21/04 ,  C12N9/10 ,  C12P21/04 ,  C12N5/00 ,  C12N15/00 ,  A23L1/236

CPC classification number: C12P19/56 ,  A23L27/36 ,  C12N9/1051 ,  C12N9/1077 ,  C12N15/52 ,  C12N15/8243 ,  C12N15/8245 ,  C12P7/42 ,  C12P15/00 ,  C12Y204/01126 ,  Y02P20/52

Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

Abstract translation: 公开了重组微生物，植物和植物细胞，其被工程化以表达编码甜菊醇生物合成酶和UDP-糖基转移酶（UGT）的新型重组基因。 这样的微生物，植物或植物细胞可以产生甜菊醇或甜菊糖苷，例如可以在食品和膳食补充剂中用作天然甜味剂的罗红糖苷或雷鲍迪苷A.

公开(公告)号：WO2007103989A8

公开(公告)日：2009-07-23

申请号：PCT/US2007063515

申请日：2007-03-07

Applicant: VERENIUM CORP ,  BURKE ELLEN ,  HICKS PAULA M ,  LUGINBUHL PETER ,  RICHARDSON TOBY ,  WEINER DAVID P ,  ZHAO LISHAN

Inventor： BURKE ELLEN ,  HICKS PAULA M ,  LUGINBUHL PETER ,  RICHARDSON TOBY ,  WEINER DAVID P ,  ZHAO LISHAN

IPC: C12N9/10

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

公开(公告)号：WO2006066072A3

公开(公告)日：2009-04-23

申请号：PCT/US2005045575

申请日：2005-12-16

Applicant: CARGILL INC ,  BRAZEAU BRIAN ,  DE TROOSTEMBERGH JEAN-CLAUDE ,  HICKS PAULA M ,  JESSEN HOLLY ,  MCFARLAN SARA C ,  OBYN WILLY ,  SANCHEZ-RIERA FERNANDO A ,  SCHROEDER WILLIAM A

Inventor： BRAZEAU BRIAN ,  DE TROOSTEMBERGH JEAN-CLAUDE ,  HICKS PAULA M ,  JESSEN HOLLY ,  MCFARLAN SARA C ,  OBYN WILLY ,  SANCHEZ-RIERA FERNANDO A ,  SCHROEDER WILLIAM A

IPC: C12N1/20 ,  C12N9/02 ,  C12P7/58

CPC classification number: C12P7/58 ,  C12P17/181 ,  C12P19/02

Abstract: A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono- -lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono- -lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.

Abstract translation: 公开了一种增加肌醇加氧酶比活性的方法。 该方法包括在有效提高肌醇加氧酶比活性的条件下，将包含肌醇加氧酶和不含硫的还原剂的混合物进行温育。 还公开了D-葡萄糖醛酸和葡萄糖醛酸内酯的制备方法，包括在有效地将每升混合物形成5克D-葡萄糖醛酸的条件下孵育包括肌醇，肌醇加氧酶和氧气的混合物至400克 D-葡萄糖醛酸每升混合物。 葡萄糖醛酸内酯可以由D-葡萄糖醛酸产物生产。 还公开了适用于这种方法的生物体和核酸。

公开(公告)号：WO2006116487A3

公开(公告)日：2008-10-30

申请号：PCT/US2006015790

申请日：2006-04-26

Applicant: CARGILL INC ,  HICKS PAULA M ,  MCFARLAN SARA C

Inventor： HICKS PAULA M ,  MCFARLAN SARA C

IPC: C12P13/22 ,  C12N1/18 ,  C12P17/10

CPC classification number: C12P17/10 ,  C12N15/52

Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

Abstract translation: 提供可用于从葡萄糖，色氨酸，吲哚-3-乳酸，吲哚-3-丙酮酸和2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸制备莫纳甜的方法和组合物 。 还公开了制备吲哚-3-丙酮酸和2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸中间体的方法。 提供的组合物包括核酸分子，多肽，化学结构和细胞。 方法包括体外和体内过程，体外方法包括化学反应。

公开(公告)号：WO2008085575A2

公开(公告)日：2008-07-17

申请号：PCT/US2007/081932

申请日：2007-10-19

Applicant: CARGILL, INCORPORATED ,  BRAZEAU, Brian, J. ,  DE SOUZA, Mervyn, L. ,  GORT, Steven, J. ,  HICKS, Paula, M. ,  KOLLMANN, Sherry, R. ,  LAPLAZA, Jose, M. ,  MCFARLAN, Sara, C. ,  SANCHEZ-RIERA, Fernando, A. ,  SOLHEID, Christopher

Inventor： BRAZEAU, Brian, J. ,  DE SOUZA, Mervyn, L. ,  GORT, Steven, J. ,  HICKS, Paula, M. ,  KOLLMANN, Sherry, R. ,  LAPLAZA, Jose, M. ,  MCFARLAN, Sara, C. ,  SANCHEZ-RIERA, Fernando, A. ,  SOLHEID, Christopher

IPC: C07H21/04 ,  C12N1/20 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/90 ,  C12P13/02 ,  C12P13/22

CPC classification number: C12N9/1096 ,  C12N9/90 ,  C12P17/10

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Abstract translation: 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产在特定莫纳甜合成途径中形成的包括一些生物合成途径的中间体R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸。

公开(公告)号：WO2006113897A3

公开(公告)日：2007-03-22

申请号：PCT/US2006014968

申请日：2006-04-20

Applicant: CARGILL INC ,  LAPLAZA JOSE ,  ANDERSON JAMES C ,  DESOUZA MERVYN L ,  HICKS PAULA M ,  KOLLMANN SHERRY R

Inventor： LAPLAZA JOSE ,  ANDERSON JAMES C ,  DESOUZA MERVYN L ,  HICKS PAULA M ,  KOLLMANN SHERRY R

IPC: G01N33/53

CPC classification number: C12P17/10 ,  A61K31/7048

Abstract: Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.

Abstract translation: 提供了莫纳甜甜味剂体内生产的产品和方法。 产品包括经遗传修饰以分泌或改善莫纳甜分泌的微生物; 经遗传修饰以产生莫纳甜的微生物; 和经遗传修饰以分泌或改善莫纳甜分泌并产生莫纳甜的微生物。 这些方法包括在这种基因工程微生物中产生莫纳甜。