公开(公告)号：WO2007103989A9

公开(公告)日：2007-11-29

申请号：PCT/US2007063515

申请日：2007-03-07

Applicant: VERENIUM CORP ,  BURKE ELLEN ,  HICKS PAULA M ,  LUGINBUHL PETER ,  RICHARDSON TOBY ,  WEINER DAVID P ,  ZHAO LISHAN

Inventor： BURKE ELLEN ,  HICKS PAULA M ,  LUGINBUHL PETER ,  RICHARDSON TOBY ,  WEINER DAVID P ,  ZHAO LISHAN

IPC: C12N9/10

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S, R configurations, and salts thereof.

Abstract translation: 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和某些莫纳甜立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。

公开(公告)号：WO2007133184A2

公开(公告)日：2007-11-22

申请号：PCT/US2006/015930

申请日：2006-04-26

Applicant: CARGILL, INCORPORATED ,  BRAZEAU, Brian J. ,  BURKE, Ellen ,  DE SOUZA, Mervyn ,  GORT, Steven J. ,  HICKS, Paula M. ,  KOLLMAN, Sherry R. ,  LUGINBUHL, Peter ,  MCFARLAN, Sara, C. ,  RICHARDSON, Toby ,  SANCHEZ-RIERA, Fernando A. ,  SOLHEID, Christopher ,  WEINER, David ,  ZHAO, Lishan

Inventor： BRAZEAU, Brian J. ,  BURKE, Ellen ,  DE SOUZA, Mervyn ,  GORT, Steven J. ,  HICKS, Paula M. ,  KOLLMAN, Sherry R. ,  LUGINBUHL, Peter ,  MCFARLAN, Sara, C. ,  RICHARDSON, Toby ,  SANCHEZ-RIERA, Fernando A. ,  SOLHEID, Christopher ,  WEINER, David ,  ZHAO, Lishan

IPC: H04L9/00

CPC classification number: C12N9/1096 ,  C12N9/90 ,  C12P17/10

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Abstract translation: 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产在特定莫纳甜合成途径中形成的包括一些生物合成途径的中间体R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸。

公开(公告)号：WO2007133183A2

公开(公告)日：2007-11-22

申请号：PCT/US2006/015913

申请日：2006-04-27

Applicant: CARGILL, INCORPORATED ,  BRAZEAU, Brian J. ,  BURKE, Ellen ,  DE SOUZA, Mervyn ,  GORT, Steven J. ,  HICKS, Paula M. ,  KOLLMAN, Sherry R. ,  LUGINBUHL, Peter ,  MCFARLAN, Sara C. ,  RICHARDSON, Toby ,  SANCHEZ-RIERA, Fernando A. ,  SOLHEID, Christopher ,  WEINER, David ,  ZHAO, Lishan

Inventor： BRAZEAU, Brian J. ,  BURKE, Ellen ,  DE SOUZA, Mervyn ,  GORT, Steven J. ,  HICKS, Paula M. ,  KOLLMAN, Sherry R. ,  LUGINBUHL, Peter ,  MCFARLAN, Sara C. ,  RICHARDSON, Toby ,  SANCHEZ-RIERA, Fernando A. ,  SOLHEID, Christopher ,  WEINER, David ,  ZHAO, Lishan

IPC: H04L9/00

CPC classification number: C12P17/10 ,  C12N9/1096 ,  C12N9/90

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Abstract translation: 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸，一种在某些monatin合成途径（包括一些生物合成途径）中形成的中间体。

公开(公告)号：WO2007103389A9

公开(公告)日：2007-11-01

申请号：PCT/US2007005718

申请日：2007-03-07

Applicant: CARGILL INC ,  BURKE ELLEN ,  GORT STEVEN JOHN ,  HICKS PAULA M ,  LUGINBUHL PETER ,  MCFARLAN SARA C ,  RICHARDSON TOBY ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

Inventor： BURKE ELLEN ,  GORT STEVEN JOHN ,  HICKS PAULA M ,  LUGINBUHL PETER ,  MCFARLAN SARA C ,  RICHARDSON TOBY ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

IPC: C12P17/00 ,  A23L27/00 ,  A23L27/30 ,  C07D209/20 ,  C12N9/88

CPC classification number: C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R3R and S5R configurations, and salts thereof.

Abstract translation: 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的一些立体异构体，例如R3R和S5R构型及其盐。

公开(公告)号：WO2005042756A3

公开(公告)日：2005-11-24

申请号：PCT/US2004034558

申请日：2004-10-21

Applicant: CARGILL INC ,  MCFARLAN SARA C ,  HICKS PAULA M ,  ZIDWICK MARY JO ,  SANCHEZ-RIERA FERNANDO A ,  CAMERON DOUGLAS C ,  DESOUZA MERVYN L ,  ROSAZZA JACK ,  GORT STEVEN J

Inventor： MCFARLAN SARA C ,  HICKS PAULA M ,  ZIDWICK MARY JO ,  SANCHEZ-RIERA FERNANDO A ,  CAMERON DOUGLAS C ,  DESOUZA MERVYN L ,  ROSAZZA JACK ,  GORT STEVEN J

IPC: C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12P13/04 ,  C12P17/10

CPC classification number: C12N9/88 ,  C12P17/10 ,  Y02P20/582

Abstract: Methods and compositions that can be used to make monatin or salt thereof from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

Abstract translation: 可用于由葡萄糖，色氨酸，吲哚-3-乳酸，吲哚-3-丙酮酸和2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸制备莫纳甜或其盐的方法和组合物 ，提供。 还公开了生产吲哚-3-丙酮酸和2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸中间体的方法。 提供的组合物包括核酸分子，多肽，化学结构和细胞。 方法包括体外和体内过程，体外方法包括化学反应。

公开(公告)号：WO2002074926A3

公开(公告)日：2002-09-26

申请号：PCT/US2002/008404

申请日：2002-03-19

Applicant: CARGILL INCORPORATED ,  SCHROEDER, William, A. ,  HICKS, Paula M. ,  MCFARLAN Sara C. ,  ABRAHAM, Timothy W.

Inventor： SCHROEDER, William, A. ,  HICKS, Paula M. ,  MCFARLAN Sara C. ,  ABRAHAM, Timothy W.

IPC: C12N9/02

Abstract: The invention provides methods and materials related to the production of organic products such as glucuronic acid, ascorbic acid, and glucaric acid. Specifically, the invention provides cells, methods for culturing cells, isolated nucleic acid molecules, and methods and materials for producing various organic products such as glucuronic acid, ascorbic acid, and glucaric acid.

公开(公告)号：WO2013022989A3

公开(公告)日：2013-02-14

申请号：PCT/US2012/050021

申请日：2012-08-08

Applicant: EVOLVA SA ,  HOUGHTON-LARSEN, Jens ,  HICKS, Paula, M. ,  NAESBY, Michael ,  OSTERGAARD, Tange, Thomas ,  HANSEN, Jorgen ,  DALGAARD MIKKELSEN, Michael ,  HALKJAER, Hansen, Esben ,  SIMON, Ernesto

Inventor： HOUGHTON-LARSEN, Jens ,  HICKS, Paula, M. ,  NAESBY, Michael ,  OSTERGAARD, Tange, Thomas ,  HANSEN, Jorgen ,  DALGAARD MIKKELSEN, Michael ,  HALKJAER, Hansen, Esben ,  SIMON, Ernesto

IPC: C12N15/82 ,  C12N15/81 ,  C12N9/10 ,  C12N9/00 ,  C12P15/00 ,  C12N9/90

Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.

公开(公告)号：WO2013022989A2

公开(公告)日：2013-02-14

申请号：PCT/US2012050021

申请日：2012-08-08

Applicant: EVOLVA SA ,  HOUGHTON-LARSEN JENS ,  HICKS PAULA M ,  NAESBY MICHAEL ,  OSTERGAARD TANGE THOMAS ,  HANSEN JORGEN ,  DALGAARD MIKKELSEN MICHAEL ,  HALKJAER HANSEN ESBEN ,  SIMON ERNESTO

Inventor： HOUGHTON-LARSEN JENS ,  HICKS PAULA M ,  NAESBY MICHAEL ,  OSTERGAARD TANGE THOMAS ,  HANSEN JORGEN ,  DALGAARD MIKKELSEN MICHAEL ,  HALKJAER HANSEN ESBEN ,  SIMON ERNESTO

IPC: C12N15/82

CPC classification number: C12P19/56 ,  C12N9/0032 ,  C12N9/0071 ,  C12N9/0073 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8243 ,  C12P19/44 ,  Y02P20/52

Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.

Abstract translation: 公开了已经被工程化以表达编码UDP-糖基转移酶（UGT）的重组基因的重组微生物，植物和植物细胞。 这样的微生物，植物或植物细胞可以产生甜菊糖苷，例如雷鲍迪苷A和/或雷鲍迪苷D，其可以用作食品和膳食补充剂中的天然甜味剂。

公开(公告)号：WO2009088442A2

公开(公告)日：2009-07-16

申请号：PCT/US2008/013968

申请日：2008-12-22

Applicant: CARGILL, INCORPORATED ,  DE SOUZA, Mervyn L. ,  NIU, Wei ,  LAPLAZA, Jose M. ,  SOLHEID, Christopher ,  KOLLMANN, Sherry R. ,  LUNDORFF, Joshua M. ,  HICKS, Paula M. ,  MCFARLAN, Sara C. ,  SANCHEZ-RIERA, Fernando A. ,  WEINER, David P. ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin

Inventor： DE SOUZA, Mervyn L. ,  NIU, Wei ,  LAPLAZA, Jose M. ,  SOLHEID, Christopher ,  KOLLMANN, Sherry R. ,  LUNDORFF, Joshua M. ,  HICKS, Paula M. ,  MCFARLAN, Sara C. ,  SANCHEZ-RIERA, Fernando A. ,  WEINER, David P. ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin

IPC: C12P13/04

CPC classification number: C12P13/04 ,  C12P13/227

Abstract: The invention provides for isomerase (e.g., racemase) and epimerase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such isomerase (e.g., racemase) and epimerase nucleic acids and polypeptides.

Abstract translation: 本发明提供异构酶（例如消旋酶）和差向异构酶多肽以及编码这种多肽的核酸。 还提供了使用这种异构酶（例如消旋酶）和差向异构酶核酸和多肽的方法。

公开(公告)号：WO2008085575A3

公开(公告)日：2009-03-19

申请号：PCT/US2007081932

申请日：2007-10-19

Applicant: CARGILL INC ,  BRAZEAU BRIAN J ,  DE SOUZA MERVYN L ,  GORT STEVEN J ,  HICKS PAULA M ,  KOLLMANN SHERRY R ,  LAPLAZA JOSE M ,  MCFARLAN SARA C ,  SANCHEZ-RIERA FERNANDO A ,  SOLHEID CHRISTOPHER

Inventor： BRAZEAU BRIAN J ,  DE SOUZA MERVYN L ,  GORT STEVEN J ,  HICKS PAULA M ,  KOLLMANN SHERRY R ,  LAPLAZA JOSE M ,  MCFARLAN SARA C ,  SANCHEZ-RIERA FERNANDO A ,  SOLHEID CHRISTOPHER

IPC: C12P17/10 ,  A23L27/20

CPC classification number: C12N9/1096 ,  C12N9/90 ,  C12P17/10

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Abstract translation: 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产在特定莫纳甜合成途径中形成的包括一些生物合成途径的中间体R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸。