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    MULTIFUNCTIONAL PROBE-PRIMERS
    23.
    发明申请
    MULTIFUNCTIONAL PROBE-PRIMERS 审中-公开
    多功能探测器

    公开(公告)号：WO2012106668A2

    公开(公告)日：2012-08-09

    申请号：PCT/US2012/023870

    申请日：2012-02-03

    Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth J.

    Inventor： LIVAK, Kenneth J.

    IPC: E21B47/06

    CPC classification number: C12Q1/6876 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2565/1015

    Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional "self-digesting" molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5'-5' orientation.

    Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 某些方法涉及编码扩增，其中靶序列与探针结合序列和任选地与索引序列相关，（2）任选的分布步骤，其中编码扩增的产物被分成多个等分试样，和（3）a 解码和检测步骤，其中确定等分试样中靶序列的存在，不存在，数量或相对量。 检测步骤使用包含以5'-5'方向连接的引物多核苷酸和探针寡核苷酸的多功能“自消化”分子探针。

    MULTIPLEX AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACID VARIATIONS
    24.
    发明申请
    MULTIPLEX AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACID VARIATIONS 审中-公开
    用于检测核酸变异体的多重扩增

    公开(公告)号：WO2011085491A1

    公开(公告)日：2011-07-21

    申请号：PCT/CA2011/000054

    申请日：2011-01-14

    Applicant: THE UNIVERSITY OF BRITISH COLUMBIA ,  FLUIDIGM CORPORATION ,  HANSEN, Carl, L., G. ,  PETRIV, Oleh ,  HEYRIES, Kevin ,  LIVAK, Kenneth, J.

    Inventor： HANSEN, Carl, L., G. ,  PETRIV, Oleh ,  HEYRIES, Kevin ,  LIVAK, Kenneth, J.

    IPC: C12Q1/68 ,  C12P19/34 ,  C40B30/04 ,  G01N33/50

    CPC classification number: C12Q1/6851 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2563/159

    Abstract: Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.

    Abstract translation: 本文提供了试剂盒，引物和方法，用于通过将生物样品分配到离散的子样品中来检测生物样品中的相对目标来源参考源比例，其中生物样品包括目标源上的多个靶分子; 和参考源上的多个参考分子; 提供指向所述多个靶分子中的一个或多个的靶引物和指向所述多个参考分子中的一个或多个的参考引物; 用目标引物和参照引物进行数字扩增; 并用靶探针检测扩增的靶产物的存在或不存在，并用参考探针检测扩增的参考产物的存在或不存在，其中扩增的靶产物与扩增的参考产物的比率指示靶源与参考源的相对量 在生物样品中。

    METHODS OF ANALYZING BINDING INTERACTIONS
    25.
    发明申请
    METHODS OF ANALYZING BINDING INTERACTIONS 审中-公开
    分析结合相互作用的方法

    公开(公告)号：WO2008005674A2

    公开(公告)日：2008-01-10

    申请号：PCT/US2007/071270

    申请日：2007-06-14

    Applicant: APPLERA CORPORATION ,  SUN, Hongye ,  LIVAK, Kenneth J.

    Inventor： SUN, Hongye ,  LIVAK, Kenneth J.

    IPC: C12Q1/68

    CPC classification number: G01N33/566 ,  G01N33/48721

    Abstract: The present disclosure relates to methods of analyzing binding interactions between a binding component and a receptor component by translocating unbound and any bound components through a pore and detecting the unbound and bound components.

    Abstract translation: 本公开涉及通过使未结合和任何结合的组分通过孔转位并检测未结合和结合的组分来分析结合组分和受体组分之间的结合相互作用的方法。

    METHODS, COMPOSITIONS, AND KITS FOR DETECTING PROTEIN AGGREGATES
    26.
    发明申请
    METHODS, COMPOSITIONS, AND KITS FOR DETECTING PROTEIN AGGREGATES 审中-公开
    用于检测蛋白质聚集体的方法，组合物和试剂盒

    公开(公告)号：WO2007128004A2

    公开(公告)日：2007-11-08

    申请号：PCT/US2007068046

    申请日：2007-05-02

    Applicant: APPLERA CORP ,  RUFF DAVID W ,  SHANNON MARK E ,  LIVAK KENNETH J ,  GUEGLER KARL J ,  HENNESSY KEVIN M

    Inventor： RUFF DAVID W ,  SHANNON MARK E ,  LIVAK KENNETH J ,  GUEGLER KARL J ,  HENNESSY KEVIN M

    IPC: C07H21/00 ,  C12N9/00

    CPC classification number: C12Q1/6876 ,  C07K14/47 ,  C07K14/4711 ,  C12Q1/6816 ,  C12Q2533/107

    Abstract: The present teachings provide methods, compositions, and kits for detecting the presence of protein aggregates. In some embodiments, the protein aggregate is treated with a labeled precursor, and the labeled precursor is incorporated into the protein aggregate to form a labeled protein aggregate. The labeled protein aggregate is then measured, thus detecting the presence of the protein aggregate. In some embodiments, the labeled protein aggregate is detected by interaction of labeled precursors, for example by a proximity ligation assay.

    Abstract translation: 本教导提供了用于检测蛋白质聚集体的存在的方法，组合物和试剂盒。 在一些实施方案中，用标记的前体处理蛋白质聚集体，并将标记的前体掺入蛋白质聚集体中以形成标记的蛋白质聚集体。 然后测量标记的蛋白质聚集体，从而检测蛋白质聚集体的存在。 在一些实施方案中，标记的蛋白质聚集体通过标记的前体的相互作用来检测，例如通过邻近连接测定。

    MULTIPLEXED AMPLIFICATION OF SHORT NUCLEIC ACIDS
    27.
    发明申请
    MULTIPLEXED AMPLIFICATION OF SHORT NUCLEIC ACIDS 审中-公开
    短期核酸的多重放大

    公开(公告)号：WO2007117256A1

    公开(公告)日：2007-10-18

    申请号：PCT/US2006/021172

    申请日：2006-05-31

    Applicant: APPLERA CORPORATION ,  LAO, Kai Qin ,  LIVAK, Kenneth, J. ,  STRAUS, Neil, A.

    Inventor： LAO, Kai Qin ,  LIVAK, Kenneth, J. ,  STRAUS, Neil, A.

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12N15/1065 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q2563/179 ,  C12Q2537/143 ,  C12Q2525/207

    Abstract: The present teachings provide methods, compositions, and kits for reverse transcribing and amplifying small nucleic acids such as micro RNAs. High levels of multiplexing are provided by the use of a zip-coded stem-loop reverse transcription primer, along with a PCR- based pre-amplification reaction that comprises a zip-coded forward primer. Detector probes in downstream decoding PCRs can take advantage of the zip-code introduced by the stem-loop reverse transcription primer. In some embodiments, further amplification is achieved by cycling the reverse transcription reaction. The present teachings also provide compositions and kits useful for performing the reverse transcription and amplification reactions described herein.

    Abstract translation: 本教导提供用于逆转录和扩增小核酸如微RNA的方法，组合物和试剂盒。 通过使用zip编码的茎环逆转录引物以及包含经翻译的正向引物的基于PCR的预扩增反应提供了高水平的复用。 下游解码PCR中的检测器探针可以利用茎环逆转录引物引入的zip码。 在一些实施方案中，通过循环逆转录反应来实现进一步的扩增。 本教导还提供可用于进行本文所述的逆转录和扩增反应的组合物和试剂盒。

    METHOD OF IDENTIFYING A NUCLEOTIDE PRESENT AT A DEFINED POSITION IN A NUCLEIC ACID
    29.
    发明申请
    METHOD OF IDENTIFYING A NUCLEOTIDE PRESENT AT A DEFINED POSITION IN A NUCLEIC ACID 审中-公开
    在核酸定义位置鉴定核苷酸的方法

    公开(公告)号：WO1992016657A1

    公开(公告)日：1992-10-01

    申请号：PCT/US1992001691

    申请日：1992-03-12

    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY ,  LIVAK, Kenneth, J. ,  RAFALSKI, Jan, Antoni ,  SHEPHERD, Nancy, Faye, Stacy

    Inventor： E.I. DU PONT DE NEMOURS AND COMPANY

    IPC: C12Q01/68

    CPC classification number: C12Q1/6876 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2535/101

    Abstract: A method is described for identifying a nucleotide at a defined point on a nucleic acid sequence. An oligonucleotide probe is annealed to a target nucleotide sequence of the nucleic acid sample at a point immediately adjacent and 3' to the nucleotide of interest. The probe is then extended in the direction of the nucleotide of interest in a reaction medium containing at least one chain terminating nucleotide triphosphate (ATP, GTP, TTP and CTP). The nucleotide of interest is complementary to the labeled nucleotide incorporated into the primer by the extension reaction.

    Abstract translation: 描述了用于鉴定核酸序列上的限定点上的核苷酸的方法。 将寡核苷酸探针与核酸样品的靶核苷酸序列在紧邻的位置退火，并与感兴趣的核苷酸3'退火。 然后将探针在含有至少一个链终止核苷酸三磷酸（ATP，GTP，TTP和CTP）的反应介质中在目标核苷酸的方向上延伸。 感兴趣的核苷酸通过延伸反应与掺入引物的标记核苷酸互补。

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