公开(公告)号：WO2006080106A1

公开(公告)日：2006-08-03

申请号：PCT/JP2005/014476

申请日：2005-08-01

Applicant: NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  UNIVERSITY OF GENEVA ,  KOHNO, Kenji ,  HERRERA, Pedro ,  NEPOTE, Virginie

Inventor： KOHNO, Kenji ,  HERRERA, Pedro ,  NEPOTE, Virginie

IPC: A01K67/027 ,  C12N15/09 ,  G01N33/15 ,  G01N33/50

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/035 ,  G01N33/5088 ,  G01N2800/042

Abstract: We prepared a transgene comprising human HB-EGF precursor cDNA, as a diphtheria toxin receptor gene, at the downstream of an insulin promoter, and introduced this transgene into a mouse fertilized egg, to produce a transgenic mouse of the present invention. In this mouse, human HB-EGF precursors are expressed specifically in islet beta cells, and by injection of diphtheria toxin, islet beta cells are selectively destroyed, resulting in that the mouse shows diabetes two or three days after the injection. This mouse can be utilized in screening and development of new medicines and therapy protocols for diabetes.

Abstract translation: 我们在胰岛素启动子的下游制备了包含人HB-EGF前体cDNA作为白喉毒素受体基因的转基因，并将该转基因引入小鼠受精卵中，从而产生本发明的转基因小鼠。 在该小鼠中，人HB-EGF前体在胰岛β细胞中特异性表达，通过注射白喉毒素，胰岛β细胞被选择性地破坏，导致小鼠在注射后两三天显示糖尿病。 该小鼠可用于筛选和开发新药和糖尿病治疗方案。

公开(公告)号：WO2005085454A3

公开(公告)日：2005-09-15

申请号：PCT/JP2005/004037

申请日：2005-03-02

Applicant: NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  RESEARCH INSTITUTE OF INNOVATIVE TECNHOLOGY FOR THE EARTH ,  KINKI UNIVERSITY ,  YOKOTA, Akiho ,  SHIGEOKA, Shigeru ,  TOMIZAWA, Ken-ichi

Inventor： YOKOTA, Akiho ,  SHIGEOKA, Shigeru ,  TOMIZAWA, Ken-ichi

IPC: C12N15/82

Abstract: An object of the present invention is to provide a transformed plant which has high photosynthesis activity, and has promoted growth and productivity as compared with a wild strain, and has no fear of diffusion of an introduced gene by pollens, by expressing a trait of a specified gene by chloroplast technology in a higher plant. According to the present invention, there is provided a transformed plant using a gene recombinant vector having an expression cassette for enhancing photosynthesis activity, containing a DNA fragment comprising a gene encoding a protein having fructose-1,6-bisphosphatase sedoheptulose-1,7-bisphosphatase activities between a nucleotide sequence complementary to the chloroplast gene rbcL and the chloroplast gene aacD.

公开(公告)号：WO2020181354A1

公开(公告)日：2020-09-17

申请号：PCT/CA2019/050319

申请日：2019-03-14

Applicant: MITSUBISHI TANABE PHARMA CORPORATION ,  MEDICAGO INC. ,  NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： LAVOIE, Pierre-Olivier ,  D'AOUST, Marc-Andre ,  KATO, Ko ,  YAMASAKI, Shotaro

IPC: C12N15/113 ,  A01H5/00 ,  C07K14/08 ,  C07K14/11 ,  C12N15/13 ,  C12N15/40 ,  C12N15/44 ,  C12N15/82 ,  C12N5/10 ,  C12P21/02

Abstract: An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbEPI42 (SEQ ID NO:1); nbSNS46 (SEQ ID NO:2); nbCSY65 (SEQ ID NO:3); nbHEL40 (SEQ ID NO:4); and nbSEP44 (SEQ ID NO:5). Methods for using the isolated expression enhancer are also provided.

公开(公告)号：WO2015093553A1

公开(公告)日：2015-06-25

申请号：PCT/JP2014/083493

申请日：2014-12-11

Applicant: RICOH COMPANY, LTD. ,  NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  NAKAGAWA, Daisuke ,  KAWAI, Norihiko ,  SATO, Tomokazu ,  YOKOYA, Naokazu

Inventor： NAKAGAWA, Daisuke ,  KAWAI, Norihiko ,  SATO, Tomokazu ,  YOKOYA, Naokazu

IPC: G06T5/00 ,  G06T1/00

CPC classification number: G06T3/0062 ,  G06T2207/10004

Abstract: An image generating apparatus for generating an output image based on an input panorama image, includes a parameter input unit inputting an output range parameter and a correction parameter, the output range parameter designating an output range in the panorama image, the correction parameter designating a correction part to be corrected in the output image; and an image correction unit correcting the correction part designated by the correction parameter in the output image. Further, the image correction unit calculates a similarity between the correction part and peripheral pixels and corrects the correction part based on the similarity and the peripheral pixels, and the output image is generated from the output range of the panorama image and is the panorama image corrected by the correction.

Abstract translation: 一种用于基于输入的全景图像生成输出图像的图像生成装置，包括输入输出范围参数和修正参数的参数输入单元，指定全景图像中的输出范围的输出范围参数，指定校正的校正参数 在输出图像中要校正的部分; 以及图像校正单元，校正由输出图像中的校正参数指定的校正部分。 此外，图像校正单元计算校正部分和周边像素之间的相似度，并且基于相似度和周边像素校正校正部分，并且从全景图像的输出范围生成输出图像，并且是校正的全景图像 通过修正。

公开(公告)号：WO2009119862A2

公开(公告)日：2009-10-01

申请号：PCT/JP2009/056409

申请日：2009-03-23

Applicant: Kao Corporation ,  NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  ARA, Katsutoshi ,  MORIMOTO, Takuya ,  OGASAWARA, Naotake

Inventor： ARA, Katsutoshi ,  MORIMOTO, Takuya ,  OGASAWARA, Naotake

IPC: C12N15/09

CPC classification number: C12N15/1082 ,  C12N15/102

Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5'-side region outside of the target region in the host DNA, a 3'-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3'-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5'-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3'-side region derived from the host DNA and the 3'-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter: and a selectable marker cassette for use in the method.

Abstract translation: 使用供体DNA修饰宿主DNA中的靶区域的方法：其中具有与宿主DNA中的靶区域外侧的5'-侧区域同源的区域的供体DNA，靶标外的3'侧区域 区域，宿主DNA中的靶区域内的第一同源重组区域，并且还具有第一选择标记基因，表达诱导启动子和第二选择标记基因 控制与3'侧区域同源的区域与与第一同源重组区域同源的区域之间的表达诱导启动子; 该方法具有以下步骤：在5'-侧区域和第一同源重组区域的区域上进行供体DNA和宿主DNA之间的同源重组的第一步骤，以进行与供体DNA整合的宿主的选择 基于第一选择标记基因的表达; 以及在源自宿主DNA的3'侧区域的两个区域和源自供体DNA的3'侧区域之间，在与供体DNA整合的第1步的宿主DNA内进行同源重组的第2步骤 ，用于在表达诱导启动子的表达诱导条件下，基于第二选择标记基因的表达来选择其目标区域被修饰的宿主;以及用于该方法的可选择标记盒。

公开(公告)号：WO2007072224A2

公开(公告)日：2007-06-28

申请号：PCT/IB2006/004043

申请日：2006-09-13

Applicant: NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HASHIMOTO, Takashi ,  KAJIKAWA, M.

Inventor： HASHIMOTO, Takashi ,  KAJIKAWA, M.

CPC classification number: C12N15/8243 ,  C07K14/415

Abstract: Four genes, A622, NBBl, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBBl, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and NBBl.

Abstract translation: 可以影响四种基因A622，NBB1，PMT和QPT，以增加烟草属植物中烟碱型生物碱的含量，以及在非尼古丁生产植物和细胞中合成烟碱型生物碱。 特别地，过表达A622，NBB1，PMT和QPT中的一种或多种可用于增加烟草植物中的尼古丁和烟碱型生物碱水平。 非尼古丁生产细胞可通过过表达A622和NBB1进行工程改造来产生尼古丁和相关化合物。

公开(公告)号：WO2006016429A1

公开(公告)日：2006-02-16

申请号：PCT/JP2005/002534

申请日：2005-02-10

Applicant: NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  KAZUSA DNA RESEARCH INSTITUTE ,  INAGAKI, Naoyuki ,  SHIMADA, Tadayuki ,  TORIYAMA, Michinori ,  OHARA, Osamu ,  NAGASE, Takahiro

Inventor： INAGAKI, Naoyuki ,  SHIMADA, Tadayuki ,  TORIYAMA, Michinori ,  OHARA, Osamu ,  NAGASE, Takahiro

IPC: C12N15/09

CPC classification number: C07K14/47 ,  A61K48/00 ,  A61L2430/32 ,  C07K14/4705 ,  C07K16/18

Abstract: A novel molecule Shootin1 was identified, whose expression changes before and after formation of polarity in nerve cells. Shootin1 is localized in the growth cone of the axon tip, which is crucial for axon formation and elongation. Shootin1 is expressed specifically in the brain and its expression significantly increases during periods when axon formation is active in individuals. Shootin1 was found to be present at high concentrations in the growth cone of axon tips, and when expressed exogenously in cultured nerve cells, Shootin1 induced formation of a plurality of axons. Because Shootin1 has such axon formation inducing function, it is possible to induce and accelerate axon formation and elongation in nerve cells, by positively controlling the expression or activity of Shootin1 or its splicing variants.

Abstract translation: 鉴定出新型分子Shootin1，其表达在神经细胞中形成极性之前和之后发生变化。 Shootin1位于轴突生长锥中，这对于轴突形成和伸长是至关重要的。 Shootin1在脑中特异性表达，其表达在个体轴突形成活跃期间显着增加。 发现Shootin1在轴突生长锥中以高浓度存在，并且当在培养的神经细胞中外源表达时，Shootin1诱导形成多个轴突。 由于Shootin1具有这样的轴突形成诱导功能，可以通过积极地控制Shootin1或其剪接变体的表达或活性来诱导和加速神经细胞的轴突形成和延长。

公开(公告)号：WO2008015551A2

公开(公告)日：2008-02-07

申请号：PCT/IB2007/002218

申请日：2007-08-01

Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA ,  NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  UECHI, Masaaki ,  SASAKI, Kazuya ,  KOBANA, Masumi ,  NISHITANI, Hirokazu ,  KOSAKA, Hiroaki

Inventor： UECHI, Masaaki ,  SASAKI, Kazuya ,  KOBANA, Masumi ,  NISHITANI, Hirokazu ,  KOSAKA, Hiroaki

IPC: B60K28/02

CPC classification number: B60T7/22 ,  B60W40/08 ,  B60W2050/0014

Abstract: An operation assist apparatus (1) according an embodiment of the invention includes: an assist controller (34, 22) that assists a driver of a vehicle in operating the vehicle before a stop point; and a watching action detector (20, 16) that detects a watching action of the driver for watching the stop point. The assist controller (34, 22) changes an assist manner in which to assist the vehicle operation by the driver in accordance with whether the watching action is detected by the watching action detector (20, 16) during deceleration of the vehicle. Thus configured, the operation assist apparatus can accurately determine whether the driver will stop the vehicle at the stop point and thus can perform the vehicle operation assist appropriately.

Abstract translation: 根据本发明的实施例的操作辅助装置（1）包括：辅助控制器（34,22），其在停止点之前协助车辆的驾驶员操作车辆; 以及检测驾驶员观看停止点的观看动作的观察动作检测器（20,16）。 根据在车辆减速期间是否由观察动作检测器（20,16）检测到观看动作，辅助控制器（34,22）改变辅助驾驶员进行车辆操作的辅助方式。 如此构成，操作辅助装置可以准确地判断驾驶员是否会在停止点停车，能够适当地进行车辆的操作辅助。

公开(公告)号：WO2006027865A2

公开(公告)日：2006-03-16

申请号：PCT/JP2005/004899

申请日：2005-03-14

Applicant: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH ,  NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  TOMIZAWA, Ken-ichi ,  KANAMOTO, Hirosuke ,  YOKOTA, Akiho ,  ASAO, Hiroshi

Inventor： TOMIZAWA, Ken-ichi ,  KANAMOTO, Hirosuke ,  YOKOTA, Akiho ,  ASAO, Hiroshi

IPC: C12N15/82 ,  A01H5/00

CPC classification number: A01H4/001 ,  C12N5/0025 ,  C12N15/8201 ,  C12N15/8214

Abstract: An object of present invention is to provide a transformation method which can effectively express a useful protein such as a medical protein in a composite plant chloroplast without producing a harmful secondary metabolite. A vector having a promoter of RNA operon derived from a composite plant chloroplast genome and a terminator of a psbA gene between a ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene and an acetyl CoA carboxylase subunit gene, and having a nucleotide sequence encoding an expression protein between the promoter and the terminator is introduced into a chloroplast of a composite plant.

Abstract translation: 本发明的目的是提供一种能够有效地在复合植物叶绿体中表达有用的蛋白质如医学蛋白质而不产生有害的次级代谢物的转化方法。 具有衍生自复合植物叶绿体基因组的RNA操纵子的启动子的载体和在核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基基因和乙酰辅酶A羧化酶亚基基因之间的psbA基因的终止子，并具有核苷酸序列 将启动子和终止子之间的表达蛋白编码引入复合植物的叶绿体中。

公开(公告)号：TWI456061B

公开(公告)日：2014-10-11

申请号：TW097118119

申请日：2008-05-16

Applicant: 出光興產股份有限公司 ,  IDEMITSU KOSAN CO., LTD. ,  國立大學法人奈良先端科學技術大學院大學 ,  NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  國立大學法人帶廣畜產大學 ,  NATIONAL UNIVERSITY CORPORATION, OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE

Inventor： 澤田和敏 ,  SAWADA, KAZUTOSHI ,  吉田和哉 ,  YOSHIDA, KAZUYA ,  松井健史 ,  MATSUI, TAKESHI ,  牧野壯一 ,  MAKINO, SOU-ICHI ,  川本惠子 ,  KAWAMOTO, KEIKO

IPC: C12N15/52 ,  C12N15/55 ,  C12N15/63

CPC classification number: C12N15/8258 ,  A61K39/0258 ,  A61K2039/517 ,  A61K2039/552 ,  C12N15/8257 ,  Y02A50/474