배추 품종 검정용 프라이머 및 이의 용도
    21.
    发明公开
    배추 품종 검정용 프라이머 및 이의 용도 有权
    用于识别中华人民共和国人民法院的使用权及其用途

    公开(公告)号:KR1020090005632A

    公开(公告)日:2009-01-14

    申请号:KR1020070068822

    申请日:2007-07-09

    CPC classification number: C12Q1/686

    Abstract: A primer pair for certificating the species of Chinese cabbage, a method for certificating the species of Chinese cabbage by using the primer pair, and a kit containing the primer pair are provided to allow the species of the transformed Chinese cabbage to be certificated by PCR. A primer pair for certificating the species of Chinese cabbage is selected from the group consisting of a primer pair represented by the sequences 1 and 2; a primer pair represented by the sequences 1 and 3; and a primer pair represented by the sequences 1 and 4. A method for certificating the species of Chinese cabbage comprises the steps of extracting genome DNA from Chinese cabbage; carrying out PCR by using the primer pair with a template of the extracted genome DNA; and separating the PCR product by size.

    Abstract translation: 提供大白菜品种鉴定的引物对,使用引物对鉴定大白菜品种的方法,以及含有引物对的试剂盒,通过PCR验证转化大白菜的种类。 用于鉴定大白菜种类的引物对选自由序列1和2表示的引物对; 由序列1和3表示的引物对; 以及由序列1和4表示的引物对。用于认证大白菜种的方法包括从大白菜中提取基因组DNA的步骤; 通过使用引物对与提取的基因组DNA的模板进行PCR; 并按照大小分离PCR产物。

    무로부터 분리된 자가불화합성 유전자
    22.
    发明公开
    무로부터 분리된 자가불화합성 유전자 无效
    自然不育基因分离自R. SATIVUS

    公开(公告)号:KR1020010054971A

    公开(公告)日:2001-07-02

    申请号:KR1019990055983

    申请日:1999-12-08

    Abstract: PURPOSE: The self-incompatibility genes isolated from radish (R. sativus) is provided, thereby self-incompatibility of vegetables can be decided at their young period. CONSTITUTION: The self-incompatibility genes is isolated and sequenced by the steps of: preparing flower specific expression cDNA and cloning the self-incompatibility genes; comparing the cloned genes, searching conserved regions and preparing primers; amplifying the self-incompatibility genes of radish (R. sativus) and chinese cabbage by PCR using the primers, cloning them and sequencing their nucleotide sequences to determine whether the genes are self-incompatible; obtaining various DNAs from different kinds of self-incompatible radishes by using self-incompatible specific primers; and cutting the DNAs with endonuclease to obtain PCR-FRLP patterns of self-incompatible radishes; and comparing the PCR-RFLP patterns with the PCR-RFLP determined by the conventional method.

    Abstract translation: 目的:提供从萝卜(R. sativus)分离的自交不亲和基因,从而可以在年轻时决定蔬菜的自交不亲和性。 构成:通过以下步骤分离和测序自交不亲和基因:制备花特异性表达cDNA并克隆自不相容性基因; 比较克隆的基因,寻找保守区域并制备引物; 使用引物通过PCR扩增萝卜(R. sativus)和大白菜的自交不亲和基因,克隆并测序其核苷酸序列,以确定基因是否是不相容的; 通过使用自不相容的特异性引物从不同种类的不相容萝卜获得各种DNA; 并用内切核酸酶切割DNA以获得自交不饱和萝卜的PCR-FRLP模式; 并将PCR-RFLP模式与通过常规方法测定的PCR-RFLP进行比较。

    흑미 품종을 판별을 위한 마커 및 이의 용도

    公开(公告)号:KR102228064B1

    公开(公告)日:2021-03-15

    申请号:KR1020190150744

    申请日:2019-11-21

    Abstract: 본발명은흑미를판별하기선별하기위한프라이머세트. 이를포함하는키트및 상기프라이머세트를이용하여흑미를판별하는방법을제공한다. 본발명의프라이머세트를사용하면영양생장후 출수및 등숙이이루어진이후에종피의색을확인할수 있는기존의선발방법에비해흑미여부판별을유묘상태에서가능하게해주며, 또한형질의동형/이형판별을가능케하여흑미의조기선발및 최소자원을활용한육종효율을증진시키는장점이있다.

Patent Agency Ranking