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公开(公告)号：KR101424192B1
公开(公告)日：2014-07-28
申请号：KR1020110017226
申请日：2011-02-25
Applicant: (주)바이오니아
CPC classification number: C12Q1/686 , C12Q1/6848 , C12Q2527/107 , C12Q2537/161 , C12Q2537/163 , C12Q2549/101 , C12Q2525/204 , C12Q2525/186
Abstract: 본 발명은 핫스타트 PCR용 조성물에 관한 것으로서, 보다 상세하게는 반응용 완충용액, MgCl
2 , 4종의 dNTP 및 DNA 중합효소를 포함하는 PCR용 조성물에 3' 말단의 수산기가 블로킹되고 프라이머와 상보적인 염기서열을 갖는 블로킹 올리고뉴클레오티드를 포함하는 것을 특징으로 하는 핫스타트 PCR용 조성물에 관한 것이다. 본 발명의 핫스타트 PCR용 조성물은 프라이머의 멜팅 온도 이하에서 블로킹 올리고뉴클레오티드가 해리되어 버리므로 낮은 온도에서의 프라이머-다이머 형성이나 비특이적인 반응에 의한 PCR 증폭 산물의 끌림 현상 등을 억제시켜 보다 정확한 PCR 결과물을 얻을 수 있다.-
公开(公告)号：KR101392305B1
公开(公告)日：2014-05-07
申请号：KR1020120050715
申请日：2012-05-14
Applicant: 부경대학교 산학협력단 , (주)바이오니아
Abstract: 본발명은서열번호 1 내지 4로표시된 2 쌍의프라이머세트를포함하는 WSSV(white spot syndrome virus) 및 HPV(hepatopancreatic parvovirus)의동시진단용멀티플렉스프라이머세트, 상기프라이머세트를포함하는 WSSV 및 HPV의동시진단용키트, 상기프라이머세트를이용하여 WSSV와 HPV를동시에진단하는방법, 서열번호 1 및 2로표시된프라이머세트를포함하는 WSSV 진단용프라이머세트및 서열번호 3 및 4로표시된프라이머세트를포함하는 HPV 진단용프라이머세트에관한것이다.
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公开(公告)号：KR1020130127079A
公开(公告)日：2013-11-22
申请号：KR1020120050715
申请日：2012-05-14
Applicant: 부경대학교 산학협력단 , (주)바이오니아
CPC classification number: C12Q1/04 , C12Q1/686 , C12Q2537/143 , C12Q2600/16 , G01N33/56983
Abstract: The present invention relates to a primer set containing two pairs of primer sets of sequence numbers 1-4 for simultaneously diagnosing white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV); a kit for diagnosing for simultaneously diagnosing WSSV and HPV, containing the primer set; a method for simultaneously diagnosing WSSV and HPV using the primer set; a primer set containing a primer set of sequence numbers 1 and 2 for diagnosing WSSV; and a primer set containing a primer set of sequence numbers 3 and 4 for diagnosing HPV.
Abstract translation: 本发明涉及包含两对序列号1-4的引物组的引物组，用于同时诊断白点综合征病毒（WSSV）和肝胰腺细小病毒（HPV）; 用于诊断同时诊断含有引物组的WSSV和HPV的试剂盒; 使用引物组同时诊断WSSV和HPV的方法; 包含用于诊断WSSV的序列号1和2的引物组的引物组; 以及包含用于诊断HPV的序列号3和4的引物组的引物组。
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公开(公告)号：KR1020080107926A
公开(公告)日：2008-12-11
申请号：KR1020070056324
申请日：2007-06-08
Applicant: (주)바이오니아
IPC: G01N33/18
CPC classification number: G01N33/186
Abstract: A water quality meter using a detection element is provided to improve the efficiency and precision of detection by using a small fish or a water flea as a detection element and by monitoring the only one individual per an accommodation space. A water quality meter using a detection element comprises water tubs(10, 30) which are provided with a plurality of accommodation spaces(11, 31) for accommodating a detection element separately; an lumination source which is installed at the bottom of the water tubs; heaters(19, 39) which controls the temperature of the water of the water tubs; a camera which is separated from the water tubs by a certain distance to photograph the behaviour of the detection element in the water tubs; a transmittance unit which transmits the information about the behaviour of the detection element photographed from the camera; and a display unit which displays the transmitted information.
Abstract translation: 提供了使用检测元件的水质计，通过使用小鱼或水蚤作为检测元件，并且通过仅监控每个住宿空间的个体来提高检测的效率和精度。 使用检测元件的水质计量器包括：水桶（10,30），其设置有分别容纳检测元件的多个容纳空间（11,31）; 安装在水桶底部的照明源; 加热器（19,39），其控制水桶的水的温度; 一个与水桶隔开一定距离的照相机，以拍摄水桶中检测元件的行为; 透射单元，其发送关于从照相机拍摄的检测元件的行为的信息; 以及显示单元，显示所发送的信息。
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公开(公告)号：KR1020120097793A
公开(公告)日：2012-09-05
申请号：KR1020110017226
申请日：2011-02-25
Applicant: (주)바이오니아
CPC classification number: C12Q1/686 , C12Q1/6848 , C12Q2527/101 , C12Q2549/101 , C12Q2527/107 , C12Q2537/161 , C12Q2537/163 , C12Q2525/204 , C12Q2525/186
Abstract: PURPOSE: A composition for hot-start PCR is provided to prevent DNA band smearing by primer-dimer formation or non-specific reaction. CONSTITUTION: A composition for hot-start PCR contains a blocking oligonucleotide which is complementary to a primer and has blocked hydroxyl group at 3' end. The melting temperature of the blocking oligonucleotide is lower than the primer having the complementary base sequence. The hydroxyl group site of the blocking oligonucleotide is substituted with substituent except for hydroxyl group. The substituent is C3-Space, C6-space, C12-space, C18-Space, amine, phosphate, DIG, or thiol. The composition additionally contains dye substance which is non-reactive to a template DNA. The dye substance is bromophenol blue, xylene cyanol, bromocresol, or cresol red. [Reference numerals] (AA) PCR reactant mixing step, reverse transcription step; (BB) PCR step; (CC, DD) Template; (EE) Primer + blocking primer; (FF) Primer; (GG) Blocking oligo; (HH) No amplification, DNA polymerase inactivation; (II) Amine; (JJ) Polymerase; (KK) Primer + blocking primer, denaturation; (LL) Blocking oligo; (MM) Denaturation; (NN) Annealing; (OO) Blocking oligo separation; (PP) Amplification; (QQ) Elongation
Abstract translation: 目的：提供用于热启动PCR的组合物，以通过引物二聚体形成或非特异性反应来防止DNA带涂抹。 构成：用于热启动PCR的组合物含有与引物互补并在3'末端具有封闭的羟基的封闭寡核苷酸。 封闭寡核苷酸的解链温度低于具有互补碱基序列的引物。 封闭寡核苷酸的羟基位置被除羟基以外的取代基取代。 取代基是C3-空间，C6-空间，C12-空间，C18-空间，胺，磷酸盐，DIG或硫醇。 该组合物另外含有对模板DNA无反应性的染料物质。 染料物质为溴酚蓝，二甲苯氰醇，溴甲酚或甲酚红。 （标号）（AA）PCR反应物混合步骤，逆转录步骤; （BB）PCR步骤; （CC，DD）模板; （EE）引物+封端引物; （FF）底漆; （GG）阻断寡核苷酸; （HH）无扩增，DNA聚合酶失活; （二）胺 （JJ）聚合酶; （KK）引物+封闭引物，变性; （LL）阻断寡核苷酸; （MM）变性; （NN）退火; （OO）阻断寡聚分离; （PP）扩增; （QQ）伸长率
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Patent Agency Ranking
公开(公告)号：KR101886274B1
公开(公告)日：2018-08-08
申请号：KR1020170057613
申请日：2017-05-08
Applicant: (주)바이오니아 , 대한민국(관리부서 질병관리본부장)
CPC classification number: Y02A50/451 , C12Q1/701 , C12Q1/6806
Abstract: 본발명은메르스코로나바이러스의 upE 유전자와 ORF1a 유전자부위의특이적인프라이머와프로브를이용하여실시간 RT-PCR방법에의한메르스코로나바이러스의검출에관한것으로, 본발명에의한메르스코로나바이러스진단방법은일반적인객담검체의전처리방법보다간단하고안전하여, 2차감염위험을감소시킬수 있고, 메르스코로나바이러스에만특이적으로반응하여감염여부를신속하고정확하게확인할수 있는효과가있으며, 검체의핵산추출부터결과분석과정을모니터링할 수있어감염의위험감소를통해검사자의안전성을높일수 있다.
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公开(公告)号：KR101412123B1
公开(公告)日：2014-06-26
申请号：KR1020120050717
申请日：2012-05-14
Applicant: 부경대학교 산학협력단 , (주)바이오니아
Abstract: 본 발명은 서열번호 3 및 4로 표시된 프라이머 세트를 포함하는 HPV의 실시간 진단용 프라이머 세트, 상기 프라이머 세트를 포함하는 HPV의 실시간 진단용 키트 및 상기 프라이머 세트를 이용하여 HPV를 실시간 진단하는 방법에 관한 것이다. 에 관한 것이다.
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公开(公告)号：KR101886272B1
公开(公告)日：2018-08-08
申请号：KR1020170163683
申请日：2017-11-30
Applicant: (주)바이오니아 , 대한민국(관리부서 질병관리본부장)
CPC classification number: Y02A50/451 , C12Q1/701 , C12Q1/6806
Abstract: 본발명은메르스코로나바이러스의 upE 유전자와 ORF1a 유전자부위의특이적인프라이머와프로브를이용하여실시간 RT-PCR방법에의한메르스코로나바이러스의검출에관한것으로, 본발명에의한메르스코로나바이러스진단방법은일반적인객담검체의전처리방법보다간단하고안전하여, 2차감염위험을감소시킬수 있고, 메르스코로나바이러스에만특이적으로반응하여감염여부를신속하고정확하게확인할수 있는효과가있으며, 검체의핵산추출부터결과분석과정을모니터링할 수있어감염의위험감소를통해검사자의안전성을높일수 있다.
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公开(公告)号：KR101555534B1
公开(公告)日：2015-10-06
申请号：KR1020120138335
申请日：2012-11-30
Applicant: (주)바이오니아
Abstract: 본발명은고효율전자동단백질제조장비에관한것으로본 발명의전자동장비는투석막이구비된반응용기가장착되는단백질발현반응부와자기장인가장치를구비한단백질정제부, 멀티웰플레이트장착부, 그리고이들간의물질을이송할수 있는자동피펫장치로구성되어무세포단백질합성을다단계적으로저분자발현용액을투석막을통해교환하여고효율로무세포단백질발현반응을수행하고합성된단백질을친화테그와자성입자를이용하여전자동으로정제하고단백질에적합한완충용액으로투석하는과정을전자동으로수행하여다수의단백질들을간편하게수백마이크로그램수준으로제조할수 있는콤팩트하고경제적인전자동단백질제조장비에관한것이다.
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公开(公告)号：KR1020140107989A
公开(公告)日：2014-09-05
申请号：KR1020130022324
申请日：2013-02-28
Applicant: (주)바이오니아
Abstract: The present invention relates to a method for synthesizing an affinity tag-free cell free target protein comprising: (a) a step for mixing a template coding a target protein having an enzyme cleavage site with cell lysates; (b) a step for expressing the protein by adding a low molecular weight expression solution to the mixture; (c) a step for attaching a magnetic particle binding to an affinity tag located at the end of the expressed protein; (d) a step for separating the affinity tag and magnetic particle from the expressed target protein by treating a protease recognizing the enzyme cleavage site; and (d) a step for collecting the target protein. The method for synthesizing a protein according to the present invention applies a method for extracting a target protein to a cell free protein expression technique using the magnetic particle conjugated to the affinity tag and the protease recognizing specific sequences so as to perform expression and extraction of the tag-free target proteins at the same time, thereby being useful in terms of production of an original form of a protein without artificial sequences having a negative effect on activation and structure of the protein in producing proteins for the purpose of industries, medicine, and researches.
Abstract translation: 本发明涉及一种合成无亲和标记物的无细胞靶蛋白的方法，包括：（a）将编码具有酶切位点的靶蛋白质的模板与细胞裂解物混合的步骤; （b）通过向混合物中加入低分子量表达溶液来表达蛋白质的步骤; （c）附着与位于表达蛋白质末端的亲和标签结合的磁性粒子的步骤; （d）通过处理识别酶切割位点的蛋白质来分离亲和标签和磁性颗粒与表达的靶蛋白质的步骤; 和（d）收集靶蛋白的步骤。 根据本发明的合成蛋白质的方法将使用与亲和标签结合的磁性颗粒和蛋白酶识别特异性序列的无细胞蛋白质表达技术提取目标蛋白质的方法进行表达和提取 无标签的靶蛋白，因此在生产原始形式的蛋白质方面是有用的，没有人造序列，对于产生蛋白质的活性和结构具有负面影响，用于工业，医药和 研究。
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