Abstract:
PURPOSE: A microsatellite marker for distinguishing foreign species of Fusarium graminearum and a distinguishing method using the same are provided to quickly and accurately distinguish species and toxin of a red mold pathogen. CONSTITUTION: A primer, FgmsTG49, of sequence numbers 1 and 2 is used for specifically amplifying a microsatellite of red mold pathogen for distinguishing foreign strain, domestic strain, and mixed strain thereof among Fusarium graminearum. A method for distinguishing the foreign species among Fusarium graminearum comprises: a step of amplifying a gene using the primer; and a step of identifying the microsatellite of the amplified gene. A kit for distinguishing and diagnosing the foreign species contains the primer and a reagent for amplification.
Abstract:
PURPOSE: A microsatellite marker for distinguishing species according to the type of toxin generated from red mold pathogen and a method for distinguishing species of pathogen using the same are provided to quickly and accurately distinguish main species and toxin by analyzing PCR using FgmsGT28. CONSTITUTION: A primer, FgmsGT28, of sequence numbers 1 and 2 is able to specifically amplify a microsatellite of red mold pathogen to distinguish Fusarium graminearum which produces deoxynivalenol(DON) and Fusarium asiaticum which produces nivalenol(NIV) by PCR(Polymerization Chain Reaction). A method for distinguishing F. graminearum and F. asiaticum comprises: a step of amplifying a gene using the primer by PCR; and a step of identifying a microsatellite site of the amplified gene. A kit for diagnosing and distinguishing species of red mold pathogen contains the primer and a reagent for amplification.
Abstract:
PURPOSE: Novel ampelomyces quisqualis 94013 capable of effectively controlling plant pathogenic bacteria without inflicting damage on environment and preventing problems caused by residual pesticide, method for controlling plant pathogenic bacteria using new fungus and large-scale culturing method is provided, which can produced in high yields within a short. CONSTITUTION: The ampelomyces quisqualis 94013 (KTCC 8940P) and pore of an ampelomyces quisqualis 94013 (KTCC 8940P) strain have excellent controlling action against plant pathogenic bacteria. The pore of the ampelomyces quisqualis 94013 (KTCC 8940P) strain is obtained by spraying moisture to barley or rice particles, inoculating 1x 103 to 107 pore and culturing at 20 to 30 deg.C at pH 3.0 to 9.0 and. And aqueous suspension is sprayed to the plant infected by Collectotrichum gloeosporioides in an amount of 1x 105-1x107 pore/ml.