公开(公告)号：KR1020110082315A

公开(公告)日：2011-07-19

申请号：KR1020100002235

申请日：2010-01-11

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤인선 ,  김둘이 ,  이연희 ,  이정숙 ,  서석철 ,  이인숙

IPC: A01H5/00 ,  C07K14/415 ,  C12N15/29 ,  C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8205 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8261

Abstract: PURPOSE: A method for controlling plant shape using an Oriza sativa-derived gene is provided to transform the shape. CONSTITUTION: A method for controlling plant shape comprises a step of controlling intracellular expression level of a polypeptide having an amino acid sequence of sequence number 1. The controlling of intracellular expression level is performed by increasing or decreasing the expression of a polynucleotide encoding the polypeptide. The polynucleotide has a base sequence of sequence number 2. The polypeptide having an amino acid sequence of sequence number 1 is isolated from a meristem of Oriza sativa.

Abstract translation: 目的：提供一种使用Oriza sativa衍生基因控制植物形状的方法，以改变形状。 构成：用于控制植物形状的方法包括控制具有序列号1的氨基酸序列的多肽的细胞内表达水平的步骤。通过增加或减少编码多肽的多核苷酸的表达来进行细胞内表达水平的控制。 多核苷酸具有序列号2的碱基序列。具有序列号1的氨基酸序列的多肽是从苜蓿的分生组织中分离的。

公开(公告)号：KR1020050079085A

公开(公告)日：2005-08-09

申请号：KR1020040007202

申请日：2004-02-04

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤인선 ,  이정숙 ,  차영순

IPC: C07K14/415

CPC classification number: C07K14/415 ,  A01N65/44 ,  C07K16/16 ,  C12N15/70 ,  C12N15/8271 ,  C12N2510/02 ,  G01N33/68 ,  G01N2333/415

Abstract: 본 발명은 신규 칼슘신호전달 인산화 효소인 OsPK1과 이 효소를 암호화하는 유전자에 대한 것이다. 스트레스 처리한 벼에서 분리한 상기 유전자는 칼슘신호, 저온 및 염 스트레스에 유전자 발현이 유도되며 CIPK(CBL-interacting protein kinase) 계열의 인산화 효소를 암호화한다. 상기효소는 아기장대 칼슘센서 단백질의 일종인 calcineurin (CBL)을 인산화시키는 고유활성이 있으며, 인산화 활성은 C 말단 부위에 의해 조절된다. 본 발명에서 제공하는 인산화 효소 및 활성조절 정보는 스트레스 내성 작물 개발에 이용할 수 있다.

公开(公告)号：KR1020110019978A

公开(公告)日：2011-03-02

申请号：KR1020090077621

申请日：2009-08-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤인선 ,  김둘이 ,  이연희 ,  이정숙 ,  최소림 ,  이인숙

IPC: C12N15/52 ,  C12N9/12 ,  C12N15/63 ,  C12N15/09

Abstract: PURPOSE: A phosphorylation enzyme RAPK1N and a method for producing a plant of drought resistance using the same are provided to ensure phosphorylation enzyme activity and to culture plants in a dry environment. CONSTITUTION: A phosphorylation enzyme polypeptide RAPK1N has an amino acid of sequence number 3. A polynucleotide encoding RAPK1N has a base sequence of sequence number 4. A vector comprises a promoter and a operatively linked polynucleotide encoding RAPK1N. A method for preparing the phosphorylation enzyme RAPK1N comprises: a step of transforming a microbe with the vector and culturing; and a step of collecting RAPK1N from the culture product. A method for producing a plant with enhanced drought resistance comprises: a step of preparing an expression vector which overexpresses a polypeptide having an amino acid sequence of sequence number 1 or 3; and a step of transforming a plant with the expression vector.

Abstract translation: 目的：提供磷酸化酶RAPK1N和使用其制备耐旱植物的方法以确保磷酸化酶活性并在干燥环境中培养植物。 构成：磷酸化酶多肽RAPK1N具有序列号3的氨基酸。编码RAPK1N的多核苷酸具有序列号4的碱基序列。载体包含编码RAPK1N的启动子和可操作连接的多核苷酸。 制备磷酸化酶RAPK1N的方法包括：用载体转化微生物并培养的步骤; 以及从培养产物中收集RAPK1N的步骤。 一种具有增强抗旱性的植物的生产方法包括：制备过表达具有序列号1或3的氨基酸序列的多肽的表达载体的步骤; 以及用表达载体转化植物的步骤。

公开(公告)号：KR1020100111345A

公开(公告)日：2010-10-15

申请号：KR1020090029717

申请日：2009-04-07

Applicant: 대한민국(농촌진흥청장)

Inventor： 이정숙 ,  윤인선 ,  김정봉 ,  윤웅한 ,  서석철

IPC: C12N9/12 ,  C12N15/54 ,  C12N15/63 ,  A01H1/00

Abstract: PURPOSE: A phosphatase, DNA molecule and plant transformant containing the same are provided to specifically induce expression and enhance aspartate kinase gene expression. CONSTITUTION: A phosphatase has an amino acid of sequence number 1. A DNA molecule containing a plynucleotide sequence encoding the amino acid of sequence number 1 comprises: a polynucleotide having 262-852bp of sequence number 2; a polynucleotide sequence having 70% or more homology with the DNA; a polynucleotide sequence which is hybridized with the polynucleotide under stringent condition; or a complementary sequence or RNA sequence. The plant is rice plant.

Abstract translation: 目的：提供含有该磷酸酶，DNA分子和植物转化体以特异性诱导表达并增强天冬氨酸激酶基因表达。 构成：磷酸酶具有序列号1的氨基酸。含有编码序列号1的氨基酸的核苷酸序列的DNA分子包含：具有序列号2的262-852bp的多核苷酸; 与DNA具有70％以上同源性的多核苷酸序列; 在严格条件下与多核苷酸杂交的多核苷酸序列; 或互补序列或RNA序列。 植物是水稻植物。

公开(公告)号：KR1020100051354A

公开(公告)日：2010-05-17

申请号：KR1020080110485

申请日：2008-11-07

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤인선 ,  이연희 ,  김둘이 ,  이정숙 ,  서석철 ,  이인숙

IPC: C12N15/29 ,  C12N15/09

Abstract: PURPOSE: A controlling method of the flowering time of a plant, and a transcription factor controlling the flowering time are provided to enhance the productivity of crops by controlling the growth of the plant. CONSTITUTION: A bZIP(basic leucine zipper domain) transcription factor controls the flowering time of a plant. The transcription factor is originated from an oryza sativa, while including a base sequence with the sequence number 1. A bZIP transcription factor OREB2-D71 includes the base sequence with the sequence number 2, and a transcription activation domain. A bZIP transcription factor protein is formed with the base sequence with the sequence number 3. The control of the flowering time of the plant is performed by over-expressing the OREB2-D71 by delaying the flowering time.

Abstract translation: 目的：提供植物开花时间的控制方法和控制开花时间的转录因子，以通过控制植物的生长来提高作物的生产力。 构成：bZIP（碱性亮氨酸拉链结构域）转录因子控制植物的开花时间。 转录因子来源于稻谷，同时包含序列号为1的碱基序列.BZIP转录因子OREB2-D71包括序列号为2的碱基序列和转录激活结构域。 用序列号3的碱基序列形成bZIP转录因子蛋白质。通过延迟开花时间来过表达OREB2-D71来进行植物开花时间的控制。

公开(公告)号：KR1020070110969A

公开(公告)日：2007-11-21

申请号：KR1020060043687

申请日：2006-05-16

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤인선 ,  이정숙 ,  이연희 ,  서석철

IPC: C12N9/12 ,  C12N15/29

Abstract: A protein kinase gene is provided to increase dehydration of crop plants through its overexpression, so that the gene is useful for increasing resistance against stresses of monocotyledon and dicotyledon crop plants. A protein kinase capable of inducing dehydration of crop plants has the amino acid sequence of SEQ ID NO:1 having a protein kinase domain in the N-terminal and an activity-regulating domain in the C-terminal, is isolated from the rice and has phosphorylation activity to a bZIP transcription factor of rice ABF family. A protein kinase gene has 95th-1189th nucleotides in the nucleotide sequence of SEQ ID NO:2, wherein the expression of the protein kinase gene is induced by dry stress and salt. The environment resistance of a crop plant is controlled by overexpression or inhibition of the protein kinase gene. A transgenic plant having increased dehydration is produced by transforming a plant with an expression vector containing the protein kinase gene.

Abstract translation: 提供蛋白激酶基因以通过其过表达来增加作物的脱水，使得该基因可用于增加对单子叶植物和双子叶植物作物的抗性的抗性。 能够诱导作物植物脱水的蛋白激酶具有在N末端具有蛋白激酶结构域的C端的氨基酸序列和C-末端的活性调节结构域，并且从水稻中分离出具有 水稻ABF家族的bZIP转录因子的磷酸化活性。 蛋白激酶基因在SEQ ID NO：2的核苷酸序列中具有第95〜第1189位核苷酸，其中蛋白激酶基因的表达受干应激和盐诱导。 通过过表达或抑制蛋白激酶基因来控制作物的耐环境性。 通过用含有蛋白激酶基因的表达载体转化植物来产生脱水增加的转基因植物。

公开(公告)号：KR1020060104772A

公开(公告)日：2006-10-09

申请号：KR1020050027204

申请日：2005-03-31

Applicant: 대한민국(농촌진흥청장)

Inventor： 이정숙 ,  서석철 ,  이연희 ,  김용환 ,  허성기

IPC: C12N15/29 ,  C12N15/82

Abstract: 본 발명은 벼 유래 OsCK1 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이 형질전환체의 제조방법에 관한 것으로서, 더욱 상세하게는, 벼 내에서 과다발현시키면 벼의 벼흰잎마름병 및 벼도열병에 대한 저항성이 높아지는, 벼 유래 OsCK1을 코딩하는 서열번호 1로 기재되는 염기서열로 이루어지는 유전자, 이 유전자를 포함하는 발현벡터 pSBM-LS28, 이 발현벡터로 형질전환된 아그로박테리움 형질전환체(수탁번호:KACC 95034P) 및 벼 형질전환체 및 이 형질전환체의 제조방법에 관한 것이다.
벼, 콜린 카이나제, 형질전환체, 벼 도열병 저항성, 벼흰잎마름병 저항성