Abstract:
본 발명의 일 측면은 국화왜화바이로이드(Chrysanthemum stunt viroid)의 폴리뉴클레오티드를 센스 및 안티센스 방향으로 포함하는 벡터가 도입된, 아그로박테리움 투머파시엔스( Agrobacterium tumefaciens )로 국화를 형질전환하는 단계를 포함하는, 형질전환 국화의 제조방법 및 이에의한 벡터에 관한 것이다. 또한, 본 발명의 일 측면은 상기 벡터가 도입된, 아그로박테리움 투머파시엔스( Agrobacterium tumefaciens )와 상기 벡터가 도입되어 형질전환된, 국화왜화바이로이드 저항성 국화에 관한 것이다.
Abstract:
PURPOSE: A primer set for diagnosing MNSV from melon and watermelon and a kit are provided to definitely diagnose MNSV developed in melon and watermealon. CONSTITUTION: A primer set for diagnosing MNSV from melon and watermelon is denoted by sequence numbers 1 and 2. A primer set for simultaneously diagnosing MNSV from melon and watermelon has a primer set of sequence numbers 1 and 2 and sequence number 3 and 4. The primer set has a primer set of sequence numbers 1 and 2 and sequence number 5 and 6. A kit for diagnosing MNSV from melon and watermelon contains the primer set.
Abstract:
PURPOSE: A duplex primer for diagnosing INSV and TSWV is provided to specifically diagnose INSV and TSWV and to enhance detection limit. CONSTITUTION: A duplex primer for diagnosing INSV(impatiens necrotic spot virus) and TSWV(tomato spotted wilt virus) contains oligonucleotide primer set containing an oligonucleotide with 15 or more continuous nucleotide fragment in sequence number 1 and 15 or more continuous nucleotide fragment in sequence number 2. A kit for simultaneously diagnosing INSV and TSWV contains the primer set and reagent for reverse-transcription and amplification.