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公开(公告)号：KR101469316B1
公开(公告)日：2014-12-09
申请号：KR1020120042360
申请日：2012-04-23
Applicant: 동국대학교 산학협력단 , 고려대학교 산학협력단
Abstract: 본발명의에피토프영역에서하나의아미노산이시스테인으로치환된변형에피토프태그는상기시스테인이치환된위치에서분할되어목적단백질양 말단에융합된재조합벡터를제공하며, 상기벡터의목적단백질부위의카르복시말단위치에인테인이존재하여, 상기벡터로형질전환된형질전환체로발현된환형단백질은인테인이제거되어발현되어, 분할되었던변형에피토프태그가다시연결면서변형에피토프태그서열을포함하므로, 상기변형에피토프태그를인지하는항체를이용하여환형단백질을특이적으로검출또는분리할수 있다.
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公开(公告)号：KR1020130128885A
公开(公告)日：2013-11-27
申请号：KR1020120053028
申请日：2012-05-18
Applicant: 고려대학교 산학협력단
Inventor： 백승필
Abstract: A protein candidate sequence is acquired as a result of detecting carbonic anhydrase genes from gene database based on Thalassiosira weissflogii-derived carbonic anhydrase 3BOH. The protein candidate sequence is structurally similar to zeta-type carbonic anhydrase although the function in Plesiocystis pacifica SIR-1 is not known. The gene sequence showed high expression levels by optimizing gene and expressing the gene sequence in E. coli and is identified to have the function of zeta-type carbonic anhydrase. The gene sequence has activity at a high temperature of approximately 80-100. Therefore, the present invention relates to production and use of zeta-type carbonic anhydrase with hydrolytic activities and thermal stability of alpha-carbonic anhydrase.
Abstract translation: 作为来自基于数据库的碳酸酐酶基因，基于Thalassiosira weissflogii衍生的碳酸酐酶3BOH，获得蛋白质候选序列。 蛋白质候选序列在结构上类似于zeta型碳酸酐酶，尽管Plesiocystis pacifica SIR-1中的功能尚不清楚。 基因序列通过优化基因并在大肠杆菌中表达基因序列显示高表达水平，并被鉴定为具有ζ型碳酸酐酶的功能。 基因序列在约80-100℃的高温下具有活性。 因此，本发明涉及具有水解活性和α-碳酸酐酶热稳定性的ζ型碳酸酐酶的生产和应用。
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公开(公告)号：KR1020130119304A
公开(公告)日：2013-10-31
申请号：KR1020120042360
申请日：2012-04-23
Applicant: 동국대학교 산학협력단 , 고려대학교 산학협력단
Abstract: PURPOSE: A method for detection and separation of ring-shaped protein is provided to detect and separate the ring-shaped protein specifically using an antibody recognizing modified epitope tag. CONSTITUTION: A fusion recombinant vector is delivered to both ends of target protein by fragmentation of modified epitope tag, in which one amino acid in epitope domain is substituted with cysteine, at the site the cysteine is substituted. The recombinant vector including the modified epitope tag is used for detection and separation of ring-shaped protein.
Abstract translation: 目的：提供一种检测和分离环状蛋白质的方法，以使用识别修饰表位标签的抗体特异性检测和分离环状蛋白质。 构成：在半胱氨酸被取代的位点处，通过片段修饰的表位标签（其中表位结构域中的一个氨基酸被半胱氨酸取代），将融合重组载体递送至靶蛋白的两端。 包含修饰的表位标签的重组载体用于检测和分离环状蛋白质。
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公开(公告)号：KR1020130111185A
公开(公告)日：2013-10-10
申请号：KR1020120138741
申请日：2012-12-03
Applicant: 고려대학교 산학협력단
CPC classification number: C12M41/46 , G01J1/0295 , G01N33/48735 , C12M29/00 , C12M41/12 , C12Q1/02
Abstract: PURPOSE: An apparatus for measuring cell viability and a method for analyzing cell viability are provided to automatize measurement of viability of various cells by an image processing method and hardware using a cheap and small photoelectron component and by developing computer software, thereby saving costs and reducing errors. CONSTITUTION: An apparatus (100) for measuring cell viability comprises: a fluidic channel (130) for injection of a culture medium and cells; a light emitting device (110) which is placed at the upper portion of the fluidic channel and emits light in a fluidic channel direction; and an image sensor (140) which is placed at the lower portion of the fluidic channel and photographs a shadow image of the cells. A method for analyzing cell viability comprises the steps of: receiving the photographed shadow image from the image sensor; acquiring specific parameter from the received shadow image; and displaying the state of cell viability using the acquired parameter. [Reference numerals] (160) Analysis module
Abstract translation: 目的：提供一种用于测量细胞活力的装置和分析细胞活力的方法，通过使用廉价和小型光电子部件的图像处理方法和硬件自动测量各种细胞的存活力，并通过开发计算机软件，从而节省成本并减少 错误。 构成：用于测量细胞活力的装置（100）包括：用于注射培养基和细胞的流体通道（130）; 发光装置（110），其放置在流体通道的上部并发射流体通道方向的光; 以及放置在流体通道的下部并拍摄单元的阴影图像的图像传感器（140）。 一种用于分析细胞活力的方法包括以下步骤：从图像传感器接收所拍摄的阴影图像; 从接收的阴影图像获取特定参数; 并使用所获取的参数显示细胞活力的状态。 （附图标记）（160）分析模块
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