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    이종 DNA 바코딩 방법
    23.
    发明公开
    이종 DNA 바코딩 방법 有权
    异质DNA修复

    公开(公告)号：KR1020140111224A

    公开(公告)日：2014-09-18

    申请号：KR1020140026952

    申请日：2014-03-07

    Applicant: 서울대학교산학협력단

    Inventor： 권성훈 ,  김준회 ,  류태훈 ,  오동윤 ,  고재경

    IPC: C12Q1/68

    CPC classification number: C12N15/1065 ,  C12Q1/6837 ,  C12Q2565/513 ,  C12Q2565/514 ,  C12Q1/6834

    Abstract: Provided is a heterogeneous DNA barcoding method comprising the steps of: (a) providing a DNA microarray including DNA spots which are separated from each other by means of a barcode base sequence; (b) providing a microwell array including microwells corresponding to the spatial position of the DNA spots on the DNA microarray; (c) loading a solution of a sample including target base sequences into the microwells; (d) producing fine reactive spaces, which are formed by spatially separating the DNA spots by means of the microwells, by combining the DNA microarray with the microwell array; and (e) combining the base sequence information of the DNA spots with the base sequence information of the sample by making the target base sequence of the sample reacted with the base sequence of the DNA spots in the fine reaction space; and (f) obtaining a byproduct including the barcode base sequence by separating the DNA microarray and the microwell array.

    Abstract translation: 提供了一种异源DNA条形码方法，其包括以下步骤：（a）提供包含通过条形码碱基序列彼此分离的DNA斑点的DNA微阵列; （b）提供微孔阵列，其包括对应于DNA微阵列上的DNA斑点的空间位置的微孔; （c）将包含靶基序列的样品溶液加载到微孔中; （d）通过将DNA微阵列与微孔阵列组合而产生通过微孔空间分离DNA斑点而形成的细小反应空间; 和（e）通过使样品的靶碱基序列与微细反应空间中的DNA斑点的碱基序列反应，将DNA斑点的碱基序列信息与样品的碱基序列信息组合; 和（f）通过分离DNA微阵列和微孔阵列获得包括条形码碱基序列的副产物。

    고순도 뉴클레오타이드의 대량 생산방법
    24.
    发明公开
    고순도 뉴클레오타이드의 대량 생산방법 有权
    大量生产高纯度核子的方法

    公开(公告)号：KR1020130046356A

    公开(公告)日：2013-05-07

    申请号：KR1020120114103

    申请日：2012-10-15

    Applicant: 서울대학교산학협력단

    Inventor： 권성훈 ,  김효기 ,  이호원 ,  김성식 ,  류태훈

    IPC: C12Q1/68 ,  C12N15/10 ,  G06F19/10

    CPC classification number: C12P19/34 ,  B01J2219/00315 ,  B01J2219/00441 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00722 ,  C40B20/02 ,  C40B50/14 ,  C40B60/04 ,  C12Q1/6811 ,  C12Q1/6834

    Abstract: PURPOSE: A mass production method of a high purity nucleotide is provided to quickly and accurately isolate a microbead with a predetermined sequence and to amplify the sequence. CONSTITUTION: A mass production method of a high purity nucleotide comprises: a step of providing a sequencing substrate with a replication library of oligonucleotides existing on a solid support(S110); a step of sequencing the replication library(S120); a step of obtaining measured location information of the solid support(S130); a step of mapping pixel information of a signal generated from the solid support and the measured location information(S140); a step of extracting a desired sequence from the sequencing substrate using the mapping result(S150); and a step of amplifying a large amount of oligonucleotides on the solid support(S160). [Reference numerals] (AA) Start; (BB) End; (S110) Provide a sequencing analysis substrate with a replication library of oligos on a solid support; (S120) Sequence the replication library; (S130) Obtain the actual location information of the solid support; (S140) Map pixel information and the actual location information; (S150) Extract the solid support using a mapping result; (S160) Massively replicate by amplifying the oligos of the extracted solid support

    Abstract translation: 目的：提供高纯度核苷酸的大规模生产方法，以预定的顺序快速准确地分离微珠并扩增序列。 构成：高纯度核苷酸的批量生产方法包括：向测序底物提供存在于固体支持物上的寡核苷酸复制文库的步骤（S110）; 对复制库进行排序的步骤（S120）; 获取固体支持物的测量位置信息的步骤（S130）; 映射从固体支持物产生的信号的像素信息和所测量的位置信息的步骤（S140）; 使用映射结果从测序底物提取期望的序列的步骤（S150）; 和在固体支持物上扩增大量寡核苷酸的步骤（S160）。 （附图标记）（AA）开始; （BB）结束; （S110）在固体支持物上提供具有寡核苷酸复制文库的测序分析底物; （S120）顺序复制库; （S130）获取实体支持的实际位置信息; （S140）映射像素信息和实际位置信息; （S150）使用映射结果提取固体支持; （S160）通过扩增提取的固体支持物的寡核苷酸大量复制

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