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公开(公告)号：KR101337064B1
公开(公告)日：2013-12-09
申请号：KR1020110110018
申请日：2011-10-26
Applicant: 서울대학교산학협력단
Abstract: 본 발명은 단백질의
O- GlcNAcylation에 대한 정보를 내포하는
O- GlcNAc 펩타이드의 분리 및 동정 방법에 관한 것이다.
본 발명에 따르면, 펩타이드의 세린 또는 트레오닌 잔기에 결합되어 있는 인산기 또는
O- GlcNAc의 화학적 변형 없이 쉽고 빠르게
O- GlcNAc 펩타이드를 인산화 펩타이드와 분리해 낼 수 있을 뿐만 아니라 분리된
O- GlcNAc 펩타이드의 서열 및
O- GlcNAc의 수식화 위치를 규명할 수 있다. 따라서 본 발명은
O- GlcNAc 펩타이드의 분석을 통한 새로운 질병 메커니즘 연구, 치료제 및 진단용 마커의 개발 등에 유용하게 활용될 수 있다.-
公开(公告)号：KR1020130031464A
公开(公告)日：2013-03-29
申请号：KR1020110095050
申请日：2011-09-21
Applicant: 서울시립대학교 산학협력단 , 가톨릭대학교 산학협력단 , 서울대학교산학협력단
CPC classification number: C12Q1/6883 , C12Q2600/156
Abstract: PURPOSE: A composition for early diagnosing Alzheimer's disease is provided to easily diagnose dementia based on C methylation ratio in CpG. CONSTITUTION: A composition for early diagnosing Alzheimer's disease contains a formulation which measures methylation level of PEG1(paternally expressed gene 1) promoter. A method for early diagnosing Alzheimer's disease comprises: a step of treating bisulfite to genome DNA isolated from blood; a step of performing PCR of the genome DNA using a PEG1 promoter-specific primer; a step of determining methylation ratio of C in CpG; and a step of diagnosing dementia when the C methylation level is higher than a healthy person.
Abstract translation: 目的：提供早期诊断阿尔茨海默病的组合物，以便根据CpG中的C甲基化比例容易诊断痴呆。 构成：用于早期诊断阿尔茨海默病的组合物含有测定PEG1（家系表达基因1）启动子甲基化水平的制剂。 一种早期诊断阿尔茨海默病的方法，包括：将亚硫酸氢盐分离至从血液中分离的基因组DNA的步骤; 使用PEG1启动子特异性引物进行基因组DNA的PCR的步骤; 确定CpG中C的甲基化比例的步骤; 并且当C甲基化水平高于健康人时诊断痴呆症的步骤。
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公开(公告)号：KR102254053B1
公开(公告)日：2021-05-20
申请号：KR1020190132027
申请日：2019-10-23
Applicant: 서울대학교산학협력단 , 고려대학교 산학협력단
IPC: G01N33/68
Abstract: 본원은혈액검사를통해뇌 아밀로이드베타축적및 이와관련된질환의조기진단이가능한바이오마커를개시한다. 본원에따른마커는혈액을이용하여임상증상이나타나기전의인지장애정상군및 경도인지장애군의뇌의아밀로이드베타축적을검출할수 있어이와관련된질환의조기진단등에편리하고유용하게사용될수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020170085792A
公开(公告)日：2017-07-25
申请号：KR1020160005364
申请日：2016-01-15
Applicant: 서울대학교산학협력단
Abstract: 본원은트롬보스폰딘-1(TSP-1) 단백질또는그 유전자를유효성분으로포함하는, 알츠하이머병예방또는치료용약학조성물에관한것으로, 본발명의조성물에의하여알츠하이머병뇌에서아밀로이드베타독성으로인한수상돌기스파인손실이예방되거나회복될수 있다.
Abstract translation: 本文血小板反应蛋白-1（TSP-1）蛋白或作为涉及，阿尔茨海默氏病预防或治疗，其包括作为活性成分，水，由本发明的组合物的药物组合物由于在阿尔茨海默氏byeongnoe毒性β淀粉样蛋白的基因 可以防止或恢复脊柱丢失。
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公开(公告)号：KR1020160129444A
公开(公告)日：2016-11-09
申请号：KR1020150061626
申请日：2015-04-30
Applicant: 서울대학교산학협력단
CPC classification number: G01N33/6896 , A61B5/15 , C07K14/4711 , C07K14/81 , G01N1/10 , G01N1/30 , G01N33/68 , G16H10/40
Abstract: 본발명은혈장에 MPP 및/또는 TCEP을처리함으로써혈장내 Aβ를정량하는방법및 이를통한임상학적인지능력저하및 병리학적 Aβ축적여부를진단하는방법에관한것으로, 본발명에따른 Aβ정량방법으로측정한측정값들의 cut-off 값을통하여정상인, MCI 및 AD와뇌에서의 Aβ축적여부를판별할수 있고, AD로의진행을예측할수 있다.
Abstract translation: 本发明涉及通过用MPP和/或TCEP处理血浆来定量血浆中的Aβ的方法，以及使用定量方法诊断是否发生临床认知恶化和病理性Aβ累积的方法。 通过根据本发明的通过定量Aβ的方法测量的测量值的截止值，可以鉴定正常的，MCI和AD受试者，并确定是否发生Aβ在脑中的积累，并且可以 预测进展到AD。
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公开(公告)号：KR1020130047782A
公开(公告)日：2013-05-09
申请号：KR1020110110018
申请日：2011-10-26
Applicant: 서울대학교산학협력단
Abstract: PURPOSE: A method for isolating and identifying O-GlcNAc peptide using metal-affinity chromatography is provided to quickly and easily isolate O-GlcNAc peptide from phosphorylated peptide without chemical modification, and to identify the peptide sequence and modification site from the isolated O-GlcNAc peptide. CONSTITUTION: A method for isolating and identifying O-GlcNAc peptide comprises: a step of performing metal-affinity chromatography of a sample containing a phosphorylated peptide and O-GlcNAc peptide; and a step of adsorbing and removing the phosphorylated peptide onto a column. Metals used for metal affinity chromatography are TiO_2, ZrO_2, GaO_4, or FeO_2. The method also comprises: a step of adding a dithiothreitol(DTT) affinity tag to O-GlcNAc peptide in the sample by BEMAD method; and a step of purifying O-GlcNAc peptide using thiol affinity chromatography. The method further comprises a step of analyzing O-GlcNAc peptide by LC-MS(liquid chromatography-mass spectrometry). [Reference numerals] (AA) Target sample to be analyzed; (BB) Metal affinity chromatography; (CC) O-GlcNac peptide containing sample; (DD) BEMAD method; (EE) DTT added peptide; (FF) Thiol chromatography, LC-MS/MS mass spectrometry; (GG) O-GlcNac peptide
Abstract translation: 目的：提供使用金属亲和层析分离和鉴定O-GlcNAc肽的方法，以快速方便地将O-GlcNAc肽从磷酸化肽中分离而无需化学修饰，并从分离的O-GlcNAc中鉴定肽序列和修饰位点 肽。 构成：用于分离和鉴定O-GlcNAc肽的方法包括：对含有磷酸化肽和O-GlcNAc肽的样品进行金属亲和层析的步骤; 以及将磷酸化肽吸附并除去到柱上的步骤。 用于金属亲和层析的金属是TiO_2，ZrO_2，GaO_4或FeO_2。 该方法还包括：通过BEMAD方法向样品中的O-GlcNAc肽添加二硫苏糖醇（DTT）亲和标签的步骤; 和使用硫醇亲和层析纯化O-GlcNAc肽的步骤。 该方法还包括通过LC-MS（液相色谱 - 质谱）分析O-GlcNAc肽的步骤。 （标号）（AA）要分析的目标样品; （BB）金属亲和色谱法; （CC）O-GlcNac肽样品; （DD）BEMAD方法; （EE）DTT添加肽; （FF）硫醇色谱，LC-MS / MS质谱; （GG）O-GlcNac肽
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27.发明公开체액 내 베타-아밀로이드 자가항체의 농도를 측정하여 제2형 당뇨병을 진단하는 방법 및 이를 이용한 제2형 당뇨병 진단키트 有权用于通过测量体液中的β-淀粉样蛋白自身抗体水平和使用其的2型糖尿病的诊断试剂盒来诊断类型2糖尿病的方法
公开(公告)号：KR1020110066059A
公开(公告)日：2011-06-16
申请号：KR1020100010568
申请日：2010-02-04
Applicant: 서울대학교산학협력단
IPC: G01N33/53 , G01N33/532
Abstract: PURPOSE: A method for diagnosing type 2 diabetes by measuring the level of beta-myloid autoantibodies in the body fluid is provided to early and simply diagnose type 2 diabetes. CONSTITUTION: A method for diagnosing type 2 diabetes comprises: a step of reacting a body fluid sample to a reactor coated with an antigen protein which specifically binds to beta-amlyoid autoantibodies; a step of detecting an antigen-antibody reactant using a secondary antibody-label conjugate and chromogenic substrate solution of a label; and a step of confirming the concentration of the beta-amyloid autoantibody. The body fluid is blood. The label of the secondary antibody conjugate is HRP(horseradish peroxidase), alkaline phosphatase, cholid gold, fluorescein, or dye.
Abstract translation: 目的：通过测量体液中β-型胶体自身抗体水平来诊断2型糖尿病的方法被提供给早期并简单地诊断2型糖尿病。 构成：用于诊断2型糖尿病的方法包括：使体液样品与涂覆有特异性结合β-淀粉样自身抗体的抗原蛋白质的反应器反应的步骤; 使用第二抗体 - 标记缀合物和标记物的显色底物溶液检测抗原 - 抗体反应物的步骤; 以及确认β-淀粉样蛋白自身抗体浓度的步骤。 体液是血液。 第二抗体缀合物的标记是HRP（辣根过氧化物酶），碱性磷酸酶，胆金，荧光素或染料。
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公开(公告)号：KR1020100049363A
公开(公告)日：2010-05-12
申请号：KR1020080108490
申请日：2008-11-03
Applicant: 서울대학교산학협력단
Inventor： 묵인희
Abstract: PURPOSE: An apparatus and method for diagnosing Alzheimer diseases(AD) through measurement of vitamin D-binding protein is provided. CONSTITUTION: An apparatus for diagnosing Alzheimer contains an antibody which binds with vitamin D-binding protein, and a label which is conjugated with the antibody and generates proportional signal to total content of the antibody conjugated to vitamin D-binding protein. A bio sample is blood, serum, plasma, or platelet. The label is gold particle, color polystyrene micro particle, enzyme, fluorescent dye, conductive polymer, magnetic particle or peroxidase. A diagnosis strip(10) for Alzheimer disease comprises: a sample pad(12) receiving a liquid-phase sample; an adhesive pad(14) containing label-attached conjugate; a porous membrane pad(16) in which a binder is fixed; and an absorption pad(18) which finally receives liquid-phase sample.
Abstract translation: 目的：提供通过测量维生素D结合蛋白来诊断阿尔茨海默病（AD）的装置和方法。 构成：用于诊断阿尔茨海默病的装置含有与维生素D结合蛋白结合的抗体，以及与抗体缀合的标记，并与结合维生素D结合蛋白的抗体的总含量产生比例信号。 生物样品是血液，血清，血浆或血小板。 标签是金颗粒，彩色聚苯乙烯微粒，酶，荧光染料，导电聚合物，磁性颗粒或过氧化物酶。 用于阿尔茨海默病的诊断条（10）包括：接收液相样品的样品垫（12）; 含有附着标记的缀合物的粘合剂垫（14） 固定有粘合剂的多孔膜垫（16）; 和最后接收液相样品的吸收垫（18）。
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