公开(公告)号：KR1020130047023A

公开(公告)日：2013-05-08

申请号：KR1020110111778

申请日：2011-10-31

Applicant: 성균관대학교산학협력단

Inventor： 김진국 ,  이진선 ,  김승현 ,  최소정

IPC: C12N5/09 ,  G01N33/574 ,  G01N33/15

Abstract: PURPOSE: A novel lung cancer cell line and a kit containing the same for screening a lung cancer therapeutic agent are provided to ensure the trait of lung cancer cells and to develop a customized therapeutic agent. CONSTITUTION: A method for screening a lung cancer therapeutic agent comprises: a step of culturing a novel lung cancer cell line, SMC-L001(deposit number: KCLRF-BP-00274); a step of treating the lung cell line with a candidate material as a lung cancer therapeutic agent; a step of culturing the treated lung cancer cell line and measuring morphological, physiological, or genetic change of the lung cancer cell line; and a step of determining whether the candidate material is used as a lung cancer therapeutic agent. A kit for screening the lung cancer therapeutic agent contains the lung cancer cell line.

Abstract translation: 目的：提供一种新型肺癌细胞系和含有其的用于筛选肺癌治疗剂的试剂盒，以确保肺癌细胞的特征并开发定制的治疗剂。 构成：筛选肺癌治疗剂的方法包括：培养新型肺癌细胞系SMC-L001（保藏号：KCLRF-BP-00274）的步骤; 用候选材料作为肺癌治疗剂治疗肺细胞系的步骤; 培养经过治疗的肺癌细胞系并测量肺癌细胞系的形态学，生理学或遗传学变化的步骤; 以及确定候选材料是否用作肺癌治疗剂的步骤。 用于筛选肺癌治疗剂的试剂盒含有肺癌细胞系。

公开(公告)号：KR1020130043520A

公开(公告)日：2013-04-30

申请号：KR1020110107727

申请日：2011-10-20

Applicant: (주)차바이오텍 ,  성균관대학교산학협력단

Inventor： 정형민 ,  문성환 ,  박순정 ,  김진국 ,  이진선 ,  김승현 ,  최소정

IPC: C12N5/09 ,  C12Q1/37 ,  C12N1/02 ,  G01N33/15

Abstract: PURPOSE: A method for isolating and proliferating lung cancer cells is provided to effectively isolate and proliferate the lung cancer cells from a patient with lung cancer, and to establish a lung cancer cell line. CONSTITUTION: A method for isolating and proliferating lung cancer cells from a lung cancer tissue comprises: a step of making an incision of a lung cancer tissue from a patient in a diameter of 40-100 micrometers; a step of culturing the lung cancer tissue; a step of isolating lung cancer cells using dispase; a step of culturing the lung cancer cells in a serum-free N2 medium; and a step of culturing the lung cancer cells in a medium containing serum and growth factors.

Abstract translation: 目的：提供肺癌细胞分离和增殖的方法，以有效地从肺癌患者中分离和增殖肺癌细胞，并建立肺癌细胞系。 构成：从肺癌组织分离和增殖肺癌细胞的方法包括：从直径为40-100微米的患者切下肺癌组织的步骤; 培养肺癌组织的一个步骤; 使用分泌酶分离肺癌细胞的步骤; 在无血清N2培养基中培养肺癌细胞的步骤; 以及在含有血清和生长因子的培养基中培养肺癌细胞的步骤。