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公开(公告)号：KR1020130047023A
公开(公告)日：2013-05-08
申请号：KR1020110111778
申请日：2011-10-31
Applicant: 성균관대학교산학협력단
IPC: C12N5/09 , G01N33/574 , G01N33/15
Abstract: PURPOSE: A novel lung cancer cell line and a kit containing the same for screening a lung cancer therapeutic agent are provided to ensure the trait of lung cancer cells and to develop a customized therapeutic agent. CONSTITUTION: A method for screening a lung cancer therapeutic agent comprises: a step of culturing a novel lung cancer cell line, SMC-L001(deposit number: KCLRF-BP-00274); a step of treating the lung cell line with a candidate material as a lung cancer therapeutic agent; a step of culturing the treated lung cancer cell line and measuring morphological, physiological, or genetic change of the lung cancer cell line; and a step of determining whether the candidate material is used as a lung cancer therapeutic agent. A kit for screening the lung cancer therapeutic agent contains the lung cancer cell line.
Abstract translation: 目的：提供一种新型肺癌细胞系和含有其的用于筛选肺癌治疗剂的试剂盒，以确保肺癌细胞的特征并开发定制的治疗剂。 构成：筛选肺癌治疗剂的方法包括：培养新型肺癌细胞系SMC-L001（保藏号：KCLRF-BP-00274）的步骤; 用候选材料作为肺癌治疗剂治疗肺细胞系的步骤; 培养经过治疗的肺癌细胞系并测量肺癌细胞系的形态学，生理学或遗传学变化的步骤; 以及确定候选材料是否用作肺癌治疗剂的步骤。 用于筛选肺癌治疗剂的试剂盒含有肺癌细胞系。
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公开(公告)号：KR1020130043520A
公开(公告)日：2013-04-30
申请号：KR1020110107727
申请日：2011-10-20
Applicant: (주)차바이오텍 , 성균관대학교산학협력단
Abstract: PURPOSE: A method for isolating and proliferating lung cancer cells is provided to effectively isolate and proliferate the lung cancer cells from a patient with lung cancer, and to establish a lung cancer cell line. CONSTITUTION: A method for isolating and proliferating lung cancer cells from a lung cancer tissue comprises: a step of making an incision of a lung cancer tissue from a patient in a diameter of 40-100 micrometers; a step of culturing the lung cancer tissue; a step of isolating lung cancer cells using dispase; a step of culturing the lung cancer cells in a serum-free N2 medium; and a step of culturing the lung cancer cells in a medium containing serum and growth factors.
Abstract translation: 目的：提供肺癌细胞分离和增殖的方法，以有效地从肺癌患者中分离和增殖肺癌细胞，并建立肺癌细胞系。 构成：从肺癌组织分离和增殖肺癌细胞的方法包括：从直径为40-100微米的患者切下肺癌组织的步骤; 培养肺癌组织的一个步骤; 使用分泌酶分离肺癌细胞的步骤; 在无血清N2培养基中培养肺癌细胞的步骤; 以及在含有血清和生长因子的培养基中培养肺癌细胞的步骤。
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公开(公告)号：KR101832133B1
公开(公告)日：2018-02-27
申请号：KR1020110107727
申请日：2011-10-20
Applicant: (주)차바이오텍 , 성균관대학교산학협력단
Abstract: 본발명은환자의폐암조직으로부터효과적인폐암세포의분리및 증식을위한배양조건및 상기의방법으로배양된폐암세포를이용한환자특이적암치료제의스크리닝방법에관한것으로, 보다구체적으로 1) 환자로부터분리한폐암조직을지름 40 내지 100 μm의크기로절개하는단계, 2) 상기 1)단계의절개된조직을배양하는단계, 3) 상기 2)단계의배양된세포에디스파아제를사용하여폐암세포를분리하는단계, 4) 상기 3)단계의폐암세포를코팅물질이코팅된배양접시에서무혈청 N2 배지로배양하여폐암세포만을분리하여배양하는단계, 및 5) 상기 4)단계의분리배양된폐암세포를혈청및 성장인자를첨가한배지에서배양하여증식시키는단계를포함하는폐암조직으로부터폐암세포를분리및 증식시키는방법및 상기방법으로제조된폐암세포를이용하여환자맞춤형암치료제를스크리닝하는방법에관한것이다.
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