公开(公告)号：KR100857687B1

公开(公告)日：2008-09-08

申请号：KR1020070030228

申请日：2007-03-28

Applicant: 연세대학교 산학협력단

Inventor： 정만길 ,  이현철

IPC: A61K36/537 ,  A61P13/12

CPC classification number: C07D307/86 ,  A61K36/537

Abstract: A method for isolating magnesium lithospermate B from Salviae miltiorrhizae is provided to improve isolation yield and purification efficiency of magnesium lithospermate B with use of expensive isolation materials such as sephadex LH-20, MCI gel CHP 20 P and silica gel, so that magnesium lithospermate B is useful to treat and prevent diabetic nephropathy, microalbuminuria and uremia. A method for isolating magnesium lithospermate B from Salviae miltiorrhizae comprises the steps of: (a) cutting the roots of Salviae miltiorrhizae and extracting them with methanol at room temperature for 3 days; (b) suspending the crude extract of Salviae miltiorrhizae with water, filtering and concentrating the water extract of Salviae miltiorrhizae at 70 deg. C, and regulating pH of the water extract with 10% HCl to 3.3-3.7; (c) adding hexane into the concentrated water extract to remove organic matters, and washing the purified water extract with ethylacetate to divide into ethylacetate extract and water extract; (d) regulating pH of the water extract with 10% HCl to 4.3-4.7, adding butanol into the water extract, removing solvent from the water extract at 70 deg. C, and drying the water extract under vacuum for 5 hours to obtain pure magnesium lithospermate B; and (e) adding distilled water into the ethylacetate extract, regulating pH of the ethylacetate extract with 10% HCl to 4.3-4.7, extracting the ethylacetate extract with new ethylacetate, and concentrating and drying the ethylacetate extract to obtain magnesium lithospermate B.

Abstract translation: 提供了一种从丹参中分离白花椒素B的方法，通过使用诸如sephadex LH-20，MCI凝胶CHP 20 P和硅胶等昂贵的隔离材料，提高了苹果酸钙B的分离产率和纯化效率， 可用于治疗和预防糖尿病肾病，微量白蛋白尿和尿毒症。 从丹参中分离白花椒素B的方法包括以下步骤：（a）切割丹参的根，并在室温下用甲醇萃取3天; （b）将丹参的粗提物用水悬浮，过滤并浓缩70℃的丹参水提物。 并用10％HCl调节水提液的pH至3.3-3.7; （c）将己烷加入到浓缩水提取物中以除去有机物，用乙酸乙酯洗涤纯化水提取物，分成乙酸乙酯提取物和水提取物; （d）用10％HCl将水提取物的pH调节至4.3-4.7，向水提取物中加入丁醇，在70℃从水提取物中除去溶剂。 C，并在真空下干燥水提取物5小时，得到纯的紫罗兰淀粉B; 和（e）向乙酸乙酯提取物中加入蒸馏水，用10％HCl调节乙酸乙酯提取物的pH至4.3-4.7，用新的乙酸乙酯萃取乙酸乙酯萃取物，浓缩和干燥乙酸乙酯萃取物，得到白蜡酸镁B.