公开(公告)号：KR1020050040156A

公开(公告)日：2005-05-03

申请号：KR1020030075209

申请日：2003-10-27

Applicant: 학교법인 건국대학교 ,  재단법인서울대학교산학협력재단

Inventor： 임융호 ,  김범준 ,  조광연 ,  최경자

IPC: C12N1/20

CPC classification number: C12N1/20 ,  A01N63/02 ,  C12R1/04

Abstract: 본 발명은 신규한 방선균속 (
Streptomyces
sp.)에 속하는 신규균주 및 그 균주의 배양액을 포함하는 농용미생물제제에 대한 발명으로, 밀녹병에 대한 항균성물질을 개발하기 위하여 토양에서 분리한 방선균으로부터 항균성물질을 조사하여 농용미생물제제로의 이용에 대한 발명이다.

公开(公告)号：KR1020030063935A

公开(公告)日：2003-07-31

申请号：KR1020020004297

申请日：2002-01-24

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준 ,  국윤호 ,  김정희 ,  임성인 ,  변경희

IPC: C12N15/31

CPC classification number: Y02A50/451 ,  C12Q1/689 ,  C12Q1/683

Abstract: PURPOSE: Primers for amplifying gene hsp65 of Mycobacterium species and an identification method of Mycobacterium species using the same are provided, thereby rapidly and easily detecting and identifying Mycobacterium species. CONSTITUTION: A pair of primers for specifically amplifying gene hsp65 of Mycobacterium species have the nucleotide sequences of SEQ ID NOs: 1 and 2. A method for identifying Mycobacterium species comprises amplifying the gene hsp65 fragments of Mycobacterium species using the primers of SEQ ID NOs: 1 and 2; and subjecting the amplified gene fragments to RPLP(restriction fragment length polymorphism) analysis using the specific restriction enzyme recognition site within the amplified fragments, wherein the restriction enzyme is Xho I.

Abstract translation: 目的：提供用于扩增分枝杆菌种基因hsp65的引物和使用其的分枝杆菌属物种鉴定方法，从而快速，容易地检测和鉴定分枝杆菌。 构成：用于特异性扩增分枝杆菌属的基因hsp65的一对引物具有SEQ ID NO：1和2的核苷酸序列。用于鉴定分枝杆菌物种的方法包括使用SEQ ID NO：1的引物扩增分枝杆菌属的基因hsp65片段。 1和2; 并使用扩增片段内的特异性限制酶识别位点对扩增的基因片段进行RPLP（限制性片段长度多态性）分析，其中限制酶是Xho I.

公开(公告)号：KR1020070004176A

公开(公告)日：2007-01-09

申请号：KR1020050059552

申请日：2005-07-04

Applicant: 주식회사 효성 ,  재단법인서울대학교산학협력재단

Inventor： 조원호 ,  김기홍 ,  김범준

IPC: B82B1/00 ,  B82B3/00 ,  B82Y40/00

CPC classification number: C08K5/03 ,  C08K3/346 ,  C08K2201/011 ,  C08L67/03

Abstract: An organic modifier for forming PET/clay nano-composite is provided to have improved thermal stability and to produce the nano-composite which has silicate layer of MMT(montmorillonite) dispersed in PET resin of the composite by reacting BrEth with triphenylphosphine to have phosphonium ion only at one side of the modifier. The organic modifier is formed by the steps of: preparing a uniform solution by pouring magnesium perchlorate in a flask while feeding nitrogen gas and adding pure acetonitrile; adding 1,2-epoxy-3-phenoxypropane and 2-bromoethanol to the solution; taking and removing diethylether layer from the solution after phase separation by extraction to obtain crude solution; reacting 1,2-epoxy-3-phenoxypropane and 2-bromoethanol to obtain an intermediate BrEth; and reacting the intermediate with triphenylphosphine to synthesize the organic modifier.

Abstract translation: 提供了一种用于形成PET /粘土纳米复合材料的有机改性剂以改善热稳定性，并通过使BrEth与三苯基膦反应制备具有分散在复合材料的PET树脂中的MMT（蒙脱石）硅酸盐层的纳米复合材料，使其具有鏻离子 只在修饰语的一边。 通过以下步骤形成有机改性剂：在加入氮气并加入纯乙腈的同时，通过在烧瓶中浇注高氯酸镁制备均匀溶液; 向溶液中加入1,2-环氧-3-苯氧基丙烷和2-溴乙醇; 通过萃取相分离后从溶液中取出乙醚层，得到粗溶液; 使1,2-环氧-3-苯氧基丙烷和2-溴乙醇反应得到中间体BrEth; 并使中间体与三苯基膦反应以合成有机改性剂。

公开(公告)号：KR1020030072087A

公开(公告)日：2003-09-13

申请号：KR1020020011648

申请日：2002-03-05

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준 ,  국윤호 ,  변경희 ,  이경갑

IPC: C12N15/10

CPC classification number: Y02A50/451

Abstract: PURPOSE: An hsp 65 gene fragment of Mycobacterium species and a method for identifying Mycobacterial species using the same are provided, thereby easily and cheaply identifying Mycobacterial species. CONSTITUTION: A polynucleotide representing 604-bp fragment of the hsp 65 gene of Mycobacterium species has the nucleotide sequence selected from SEQ ID NOs: 1 to 54. A nucleotide set for identifying Mycobacterium species comprises at least one 604-bp fragment of the hsp 65 gene of Mycobacterium species selected from SEQ ID NOs: 1 to 54. A method for identifying Mycobacterial species comprises the steps of: (1) amplifying an hsp 65 gene fragment of an interest strain using a primer; (2) analyzing the nucleotide sequence of the amplified hsp 65 gene fragment; and (3) comparing the nucleotide sequence of the hsp 65 604-bp fragment of the interest strain with that of standard Mycobacterium strain.

Abstract translation: 目的：提供分枝杆菌属物种的hsp65基因片段，并提供鉴定分枝杆菌物种的方法，从而容易且廉价地鉴定分枝杆菌菌种。 构成：表达分枝杆菌属物种的hsp65基因的604-bp片段的多核苷酸具有选自SEQ ID NO：1至54的核苷酸序列。用于鉴定分枝杆菌属物种的核苷酸集合包含至少一个hsp65的604bp片段 选自SEQ ID NO：1至54的分枝杆菌种的基因。鉴定分枝杆菌物种的方法包括以下步骤：（1）使用引物扩增感兴趣菌株的hsp65基因片段; （2）分析扩增的hsp65基因片段的核苷酸序列; 和（3）比较感兴趣株的hsp 65 604-bp片段与标准分枝杆菌菌株的核苷酸序列。