公开(公告)号：KR1020100036582A

公开(公告)日：2010-04-08

申请号：KR1020080095870

申请日：2008-09-30

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준 ,  김기정 ,  이승애 ,  윤태중

IPC: C12Q1/68

CPC classification number: C12Q1/706 ,  G01N33/57438 ,  G01N2033/57461 ,  G01N2800/085

Abstract: PURPOSE: A kit for diagnosing hepatism derived from hepatitis type B through realtime PCR(polymerase chain reaction) is provided to easily detect mutated HBV preS1 antigen gene which is specific in hepatitis, hepatic cirrhosis, and liver cancer. CONSTITUTION: A kit for diagnosing hepatitis type B and hepatism derived from the hepatitis type B contains a primer having sequences of sequence numbers 1 to 4 and probe having sequences of sequence numbers 5 and 6. The kit for diagnosing hepatitis type B and hepatism derived from the hepatitis type B is used in detecting mutated preS1 antigen gene. Liver disease derived from hepatitis type B is liver cancer or liver cirrhosis.

Abstract translation: 目的：提供通过实时PCR（聚合酶链反应）诊断来自乙型肝炎肝炎的试剂盒，以便于检测肝炎，肝硬化和肝癌特异性的突变型HBV preS1抗原基因。 构成：用于诊断乙型肝炎和乙型肝炎的肝病的试剂盒含有具有序列号1至4的序列的引物和具有序列号5和6的序列的探针。用于诊断乙型肝炎和肝病的试剂盒来源于 B型肝炎用于检测突变的preS1抗原基因。 来自乙型肝炎的肝病是肝癌或肝硬化。

公开(公告)号：KR100764844B1

公开(公告)日：2007-10-08

申请号：KR1020060042174

申请日：2006-05-11

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준

IPC: G01N33/574 ,  G01N33/576 ,  G01N33/53

Abstract: A diagnostic kit for hepatic disorder derived from hepatitis B by using a HBV(hepatitis B virus) X antigen is provided to diagnose hepatic disorders including liver cirrhosis and liver cancer as well as hepatitis B by specifically detecting modified X antigen gene derived from HBV(hepatitis B virus) at an early stage. A diagnostic kit for hepatic disorder derived from hepatitis B by using a HBV X antigen comprises primers having the nucleotide sequences of SEQ ID NOs:1-3 and 5 and a restriction enzyme Fok-I. Alternatively, the diagnostic kit for hepatic disorder derived from hepatitis B by using a HBV X antigen contains a DNA chip consisting of (i) gene probes being capable of specifically binding to genes of V5M and H94Y X antigens, and having the nucleotide sequences of SEQ ID NOs: 6 to 13, (ii) a linker containing oligo (dT)15, -(CH2)6- and amine groups sequentially and linked to the 5'-terminal of the gene probes with a thymine site, and (iii) a solid substrate having an aldehyde group, which is linked to the amine group of linker through Schiff's base reaction, on the surface.

Abstract translation: 通过特异性检测来自HBV的修饰的X抗原基因（肝炎），提供通过使用HBV（乙型肝炎病毒）X抗原从乙肝引起的肝脏疾病的诊断试剂盒，用于诊断肝脏疾病，包括肝硬化和肝癌以及乙型肝炎 B病毒）。 通过使用HBV X抗原从乙型肝炎引起的肝脏疾病的诊断试剂盒包括具有SEQ ID NO：1-3和5的核苷酸序列的引物和限制酶Fok-1。 或者，通过使用HBV X抗原从乙型肝炎引起的肝病的诊断试剂盒含有DNA芯片，其包含（i）能够特异性结合V5M和H94Y X抗原的基因的基因探针，并具有SEQ ID NO： ID NOs：6〜13，（ii）连接物，其连接体依次连接到基因探针的5'末端并与胸腺嘧啶位点连接，（iii）含有寡核苷酸（dT）15， - （CH2） 在表面上通过席夫氏碱反应将具有醛基的固体底物与连接体的胺基连接。

公开(公告)号：KR100758727B1

公开(公告)日：2007-09-14

申请号：KR1020060030113

申请日：2006-04-03

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준 ,  지영미 ,  김홍 ,  문호숙

IPC: C12Q1/68 ,  G01N33/576 ,  G01N33/53

CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q2600/118 ,  G01N33/5308 ,  G01N33/5761

Abstract: A composition for diagnosis of the progress of liver disease of a HBV(Hepatitis B Virus) infected patient and a kit containing the same composition are provided to anticipate, diagnose and prevent the progress of liver disease of the HBV infected patient by confirming nucleotide deletion of a preS1 initiation site. A polynucleotide which has base deletions of 15-21 bp(2848th to 2880th bases) in the coding initiation site of preS1 gene of HBV genotype C genome genes having the nucleotide sequence of SEQ ID NO:19, and total 15-400 continuous bases located at both ends of the deletion site is provided, wherein the base deletion is 15bp base deletion from 2849th-2863th bases in the nucleotide sequence of SEQ ID NO:19, 15bp base deletion from 2850th-2864th bases in the nucleotide sequence of SEQ ID NO:19, 21bp base deletion from 2849th-2869th bases in the nucleotide sequence of SEQ ID NO:19, or 21bp base deletion from 2854th-2874th bases in the nucleotide sequence of SEQ ID NO:19. The composition for diagnosis of the progress of liver disease of a HBV infected patient comprises the probe having a nucleotide sequence complementary with the polynucleotide sequence, selected from SEQ ID NOs:9, 10, 11 and 12 to detect the polynucleotide.

Abstract translation: 提供用于诊断HBV（乙型肝炎病毒）感染患者的肝病进展的组合物和含有相同组成的试剂盒，以通过确认HBV感染患者的肝脏疾病的核苷酸缺失来预测，诊断和预防HBV感染患者的肝病进展 preS1启动点。 在具有SEQ ID NO：19的核苷酸序列的HBV基因型C基因组基因的preS1基因的编码起始位点中具有15-21bp（2848至2880个碱基）的碱基缺失的多核苷酸，以及总共15-400个连续碱基 提供缺失位点的两端，其中碱基缺失是SEQ ID NO：19的核苷酸序列中的第2849位至第2863位碱基之间的15bp碱基缺失，SEQ ID NO：1的核苷酸序列中的第2850至第2864位碱基的15bp碱基缺失 SEQ ID NO：19的核苷酸序列中的第2849〜2869位碱基有21bp的碱基缺失或SEQ ID NO：19的核苷酸序列中第2854〜2874位碱基的21bp碱基缺失。 用于诊断HBV感染患者的肝病进展的组合物包括具有与选自SEQ ID NO：9,10,11和12的多核苷酸序列互补的核苷酸序列的探针，以检测多核苷酸。

公开(公告)号：KR100435587B1

公开(公告)日：2004-06-10

申请号：KR1020020004297

申请日：2002-01-24

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준 ,  국윤호 ,  김정희 ,  임성인 ,  변경희

IPC: C12N15/31

CPC classification number: Y02A50/451

Abstract: 본 발명은 미코박테리움속 균종에 특이적인 프라이머쌍, 및 이를 이용한 미코박테리움속 균종의 동정방법에 관한 것으로서, 더욱 자세하게는 미코박테리움속 균종의 hsp65 유전자를 증폭하는 프라이머쌍, 이를 이용하여 미코박테리움속 균종의 hsp65 유전자를 PCR(Polymerase Chain Reation) 증폭하고, 증폭된 핵산내에 존재하는 특정제한효소 부위를 이용한 RFLP 분석(Restriction Fragment Length Polymorphism Anaylsis)으로 미코박테리움속 균종을 동정하는 방법에 관한 것이다.
본 발명의 프라이머쌍 및 이를 이용한 PCR-RFLP 방법은 기존의 동정 방법보다도 간편하고 저렴하며 특이적인 미코박테리움 속 균종 탐지 및 동정 방법이며, 시료내에 포함된 미코박테리움속 균의 감염여부 및 균종의 동정을 신속하고 정확히 진단할 수 있는 진단 키트에 사용될 수 있다.

Abstract translation: 目的：提供用于扩增分枝杆菌种基因hsp65的引物和使用其的分枝杆菌种的鉴定方法，从而快速且容易地检测和鉴定分枝杆菌种。 构成：用于特异性扩增分枝杆菌属物种基因hsp65的一对引物具有SEQ ID NO：1和2的核苷酸序列。用于鉴定分枝杆菌属物种的方法包括使用SEQ ID NO：1的引物扩增分枝杆菌属物种的基因hsp65片段。 1和2; 并使用扩增片段内的特定限制酶识别位点对扩增的基因片段进行RPLP（限制性片段长度多态性）分析，其中限制性内切酶是Xho I。

公开(公告)号：KR1020090033307A

公开(公告)日：2009-04-02

申请号：KR1020070098527

申请日：2007-09-29

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준

IPC: C12Q1/68

CPC classification number: C12Q1/683 ,  C12Q1/686 ,  C12Q1/6888

Abstract: A diagnostic kit for hepatitis B-associated hepatic disorder is provided to detect transformed preS2 antigenic proteins or genes derived from hepatitis B virus, which is specific to patients with hepatic disorder. A diagnostic kit for hepatitis B-associated hepatic disorder comprises primer having sequence number of SEQ ID NO:1, 2, 5 and 6 and restriction enzyme Mbo II. The hepatitis B-associated hepatic disorder indicates liver cancer or hepatic cirrhosis. An ELISA kit for diagnosing hepatitis B-associated hepatic disorder consists of: a fastening board wherein a rabbit antibody against preS2 antigenic protein(F141L preS2) is immobilized; a goat antibody solution, marked by peroxydase, against the preS2 antigenic protein(F141L preS2); and peroxide.

Abstract translation: 提供乙型肝炎相关肝病的诊断试剂盒，用于检测转化的preS2抗原蛋白或源自乙型肝炎病毒的基因，这是肝病患者特异的。 用于乙型肝炎相关肝病的诊断试剂盒包括具有SEQ ID NO：1,2,5和6以及限制酶Mbo II的序列号的引物。 乙型肝炎相关肝脏疾病表明肝癌或肝硬化。 用于诊断乙型肝炎相关性肝病的ELISA试剂盒包括：固定有针对preS2抗原蛋白（F141L preS2）的兔抗体的紧固板; 针对前S2抗原蛋白（F141L preS2）的由过氧化物酶标记的山羊抗体溶液; 和过氧化物。

公开(公告)号：KR1020080047269A

公开(公告)日：2008-05-28

申请号：KR1020070115941

申请日：2007-11-14

Applicant: 재단법인서울대학교산학협력재단 ,  재단법인 제주테크노파크

Inventor： 김범준 ,  문호숙 ,  오은주 ,  김창진

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6869

Abstract: A method for identifying a pathogenic genus of potato scab is provided to identify the pathogenic genus conveniently and economically with high accuracy by solving problems of a generally used 16S rRNA identification technique and an identification method targeting 16S-23S ITS, thereby being widely used for identifying the pathogenic genus of the potato scab. A method for identifying a pathogenic genus of potato scab comprises the steps of: (a) amplifying an rpoB gene fragment of a target strain using at least one primer selected from the group consisting of a primer including a sequence of SEQ ID : NO. 1 and a primer including a sequence of SEQ ID : NO. 2; (b) analysing the sequence of the amplified rpoB gene fragment; and (c) after multialigning the sequence analyzed by the step(b) by substituting it for a sequence of an rpoB gene selected from the group consisting of sequences of SEQ ID : NOs. 3 to 21 of a standard strain related to the potato scab, completing a genealogy to determine the genus. A method for classifying the pathogenic genus of the potato scab comprises a step of analyzing signature amino acid sequences of 336th and 442nd among an amino acid deduced from the rpoB gene sequence analyzed by the step(b).

Abstract translation: 提供了一种鉴定马铃薯斑纹病病原体的方法，通过解决一般使用的16S rRNA鉴定技术和针对16S-23S ITS的鉴定方法的问题，以高精度方便，经济地识别病原属，从而广泛用于鉴定 马铃薯痂病的病原属。 用于鉴定马铃薯斑纹病的致病属的方法包括以下步骤：（a）使用至少一种选自下组的引物扩增靶菌株的rpoB基因片段：包括SEQ ID NO： 1和包含SEQ ID NO：1的序列的引物。 2; （b）分析扩增的rpoB基因片段的序列; 和（c）通过用步骤（b）分析的序列多重标记选自SEQ ID NO：的序列的rpoB基因的序列后。 3至21个与马铃薯痂病相关的标准菌株，完成家谱以确定属。 用于分类马铃薯斑纹病的致病属的方法包括分析由步骤（b）分析的rpoB基因序列推导的氨基酸中第336位和第442位的特征氨基酸序列的步骤。

公开(公告)号：KR100705402B1

公开(公告)日：2007-04-10

申请号：KR1020050059551

申请日：2005-07-04

Applicant: 주식회사 효성 ,  재단법인서울대학교산학협력재단

Inventor： 조원호 ,  김기홍 ,  김범준

IPC: B82B1/00 ,  B82B3/00 ,  B82Y40/00

Abstract: 본 발명은 하기와 같은 구조를 갖는 신규한 나노복합재용 유기화제 및 그 제조방법에 관한 것이다.
특히, 본 발명의 신규한 나노복합재용 유기화제는 열안정성을 보다 향상시키기 위해 이미다졸늄(imidazolium) 이온이 도입된 유기화제를 설계하였다. 유기화제의 열안정성이 향상된 신규한 나노복합재용 유기화제는 박리형 PET 나노복합재 제조에 보다 유리하게 작용할 뿐만 아니라 최종적으로는 보다 큰 폭으로 PET 나노복합재의 여러 물성들을 향상시킨다.
또한, 본 발명에서 설계한 유기화제는 히드록시 그룹을 가지므로 PET 수지와 수소결합에 의한 강한 상용작용이 가능할 것이라고 예상된다.

나노복합재, 이미다졸늄, 유기화제, 점토, PET

公开(公告)号：KR1020070004175A

公开(公告)日：2007-01-09

申请号：KR1020050059551

申请日：2005-07-04

Applicant: 주식회사 효성 ,  재단법인서울대학교산학협력재단

Inventor： 조원호 ,  김기홍 ,  김범준

IPC: B82B1/00 ,  B82B3/00 ,  B82Y40/00

CPC classification number: C08K5/03 ,  C08K3/346 ,  C08K2201/011 ,  C08L67/03

Abstract: An organic modifier for forming exfoliated nano-composite is provided to improve thermal stability of the nano-composite and other physical properties of the composite by introducing imidazolium ions to the modifier and having hydroxy group to derive synergistic effect because of hydrogen bond between the hydroxy group and PET resin of the composite. The organic modifier is formed by the steps of: preparing a uniform solution by pouring magnesium perchlorate in a flask while feeding nitrogen gas and adding pure acetonitrile; adding 1,2-epoxy-3-phenoxypropane and 2-bromoethanol to the solution; taking and removing diethylether layer from the solution after phase separation by extraction to obtain crude solution; reacting 1,2-epoxy-3-phenoxypropane and 2-bromoethanol to obtain an intermediate BrEth; and reacting the intermediate with triphenylphosphine to synthesize the organic modifier. The modifier further contains imidazolium ions.

Abstract translation: 提供了一种用于形成剥离的纳米复合材料的有机改性剂，以通过将咪唑鎓离子引入改性剂并具有羟基来获得纳米复合材料的热稳定性和其他物理性能，以获得协同效应，因为羟基 和复合材料的PET树脂。 通过以下步骤形成有机改性剂：在加入氮气并加入纯乙腈的同时，通过在烧瓶中浇注高氯酸镁制备均匀溶液; 向溶液中加入1,2-环氧-3-苯氧基丙烷和2-溴乙醇; 通过萃取相分离后从溶液中取出乙醚层，得到粗溶液; 使1,2-环氧-3-苯氧基丙烷和2-溴乙醇反应得到中间体BrEth; 并使中间体与三苯基膦反应以合成有机改性剂。 改性剂还含有咪唑鎓离子。

公开(公告)号：KR100764850B1

公开(公告)日：2007-10-08

申请号：KR1020060035971

申请日：2006-04-21

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준

IPC: G01N33/576 ,  C12Q1/68

CPC classification number: G01N33/5761 ,  C12Q1/683 ,  C12Q1/6888 ,  C12Q1/706

Abstract: Diagnostic kits for hepatitis B are provided to detect genotype C hepatitis B specifically at an early stage by detecting a mutated S antigen gene of hepatitis B virus(HBV) in which a 182nd amino acid is mutated. A diagnostic kit for hepatitis B comprises (i) primers having the nucleotide sequences of SEQ ID NOs:1 to 4 and a restriction enzyme Hinf-1, or (ii) primers having the nucleotide sequences of SEQ ID NOs:18 to 21 and a restriction enzyme HphI. The diagnostic kit for hepatitis B comprises (i) gene probes capable of specifically binding to a gene encoding W182Ter S antigen and having the nucleotide sequences of SEQ ID NOs: 7 to 17, (ii) a linker containing oligo (dT)15, -(CH2)6- and amine groups sequentially and being linked to the 5'-terminal of gene probes with a thymine site, and (iii) a solid substrate having an aldehyde group, which is linked to the amine group of linker through Schiff's base reaction, on the surface. A HBV S antigen gene specific to liver cancer has one from the nucleotide sequences of SEQ ID NOs:22 to 24(Jeju-Hcc 31, 32, 44).

Abstract translation: 提供乙型肝炎诊断试剂盒，通过检测其中第182位氨基酸突变的乙型肝炎病毒（HBV）的突变型S抗原基因，在早期特异性检测基因型C型乙型肝炎。 用于乙型肝炎的诊断试剂盒包含（i）具有SEQ ID NO：1至4的核苷酸序列和限制酶Hinf-1的引物，或（ii）具有SEQ ID NO：18至21的核苷酸序列的引物和 限制酶HphI。 用于乙型肝炎的诊断试剂盒包括（i）能够特异性结合编码W182Ter S抗原的基因并具有SEQ ID NO：7至17的核苷酸序列的基因探针，（ii）含有寡核苷酸（dT）15， （CH2）6-和胺基，并与胸腺嘧啶位点连接到基因探针的5'末端，和（iii）具有醛基的固体底物，其通过席夫碱与连接体的胺基连接 反应，表面。 肝癌特异性的HBV S抗原基因具有SEQ ID NO：22〜24（Jeju-Hcc 31,32,44）的核苷酸序列之一。

公开(公告)号：KR100486820B1

公开(公告)日：2005-04-29

申请号：KR1020020011648

申请日：2002-03-05

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준 ,  국윤호 ,  변경희 ,  이경갑

IPC: C12N15/10

CPC classification number: Y02A50/451

Abstract: 본 발명은 미코박테리움속 hsp 65 유전자의 604-bp 분절을 나타내는 폴리뉴클레오타이드, 및 이들을 이용한 미코박테리움속 균주를 동정하는 방법에 관한 것이다. 본 발명의 hsp 65 유전자의 604-bp 분절은 비교염기서열 분석방법, 프로브혼성화법, 및 PCR-RFLP등의 방법에 이용가능하고, hsp 65 유전자의 604-bp 분절을 이용한 동정법은 생장속도가 느리고 다양한 균주가 존재한다는 문제점, 그리고 물질위주 동정 및 16s rDNA 동정이 갖는 문제점을 해결하여, 간편하고, 경제적이고 정확성이 높은 동정방법을 제공한다는 장점이 있어, 향후 미코박테리움속 균주의 동정에 널리 이용될 수 있다.