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公开(公告)号：KR100450633B1
公开(公告)日：2004-09-30
申请号：KR1020020011191
申请日：2002-03-02
Applicant: 정필훈
IPC: C12M3/00
CPC classification number: C12M35/02
Abstract: PURPOSE: A method and an apparatus for culturing a nerve cell by electronic stimulus are provided, thereby forming synapsis and nerve circuit between axons. CONSTITUTION: A method for culturing a nerve cell by electronic stimulus comprises adhering a nerve cell on the conductive polymer-coated silicone board which is coated with gold, and culturing the nerve cell, wherein the nerve cell adhered to the silicone board is subjected to voltage in the presence of a culturing medium during the cultivation to proliferate the nerve cell, and to induce differentiation and axons; the conductive polymer is polypyrrol and coated on the gold-coated silicone board using electric polymerization; and the nerve cell is cultured under voltage of 0.01 to 1.0 V for 1 to 4 minutes. An apparatus for culturing a nerve cell by electronic stimulus comprises a culturing vessel; a board for adhering the nerve cell; electrodes; and a pseudo electrode for maintaining the voltage of the positive(+) electrode to be constant, wherein the board is prepared by coating a gold-coated silicone board with the conductive polymer.
Abstract translation: 目的：提供一种通过电子刺激来培养神经细胞的方法和装置，从而形成轴突之间的联会和神经回路。 组成：通过电子刺激培养神经细胞的方法包括将神经细胞粘附在涂有金的导电聚合物涂覆的硅酮板上，并培养神经细胞，其中粘附在硅酮板上的神经细胞受到电压 在培养期间存在培养基以增殖神经细胞并诱导分化和轴突; 导电聚合物是聚吡咯并使用电聚合涂覆在镀金硅树脂板上; 神经细胞在0.01〜1.0V的电压下培养1〜4分钟。 通过电子刺激培养神经细胞的装置包括培养容器; 用于粘附神经细胞的板; 电极; 以及用于保持正（+）电极的电压恒定的伪电极，其中该基板通过用导电聚合物涂布镀金硅酮板来制备。
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22.发明授权치아인회석과 고분자를 이용한, 조직공학용 생체흡수성세라믹-고분자 복합 물질, 제조방법 및 이의 용도 失效치아인회석과고분자를이용한，조직공학용생체흡수성세라믹 - 고분자복합물질，제조방법및이의용도
公开(公告)号：KR100450145B1
公开(公告)日：2004-09-30
申请号：KR1020010055931
申请日：2001-09-11
Applicant: 정필훈
IPC: A61L27/12
Abstract: PURPOSE: Provided is a bioabsorptive ceramic-polymer complex material for tissue engineering with teeth apatite and polymer used which mixes teeth apatite, polymer for tissue engineering and solvent to keep porosity on the surface and section so that it improves humidity absorption rate. CONSTITUTION: The production process of the bioabsorptive ceramic-polymer complex material for tissue engineering with teeth apatite and polymer used comprises the steps of: (i) extracting an inorganic material from teeth of human or animals and crushing them to prepare teeth apatite; (ii) selecting one material from PGA, PLA, PLLA, PLGA, chitosan and PHBV as bioabsorptive polymer; (iii) selecting one solvent from methylene chloride, chloroform, DMSO and week acid; and (iv) mixing 60-90wt% of bioabsorptive polymer and 10-40wt% of teeth apatite under the selected solvent to produce a ceramic-polymer complex material.
Abstract translation: 用途：提供一种用于组织工程的生物吸收性陶瓷 - 聚合物复合材料，其具有牙齿磷灰石和使用的聚合物，其混合牙齿磷灰石，用于组织工程的聚合物和溶剂，以保持表面和截面上的孔隙率，从而提高吸湿率。 本发明提供了一种生物吸附性陶瓷 - 聚合物复合材料的制备方法，所述生物吸附性陶瓷 - 聚合物复合材料具有用于牙齿磷灰石和聚合物的组织工程，包括以下步骤：（i）从人或动物的牙齿中提取无机材料并粉碎以制备牙齿磷灰石; （ii）从PGA，PLA，PLLA，PLGA，壳聚糖和PHBV中选择一种作为生物吸收聚合物的材料; （iii）从二氯甲烷，氯仿，DMSO中选择一种溶剂并且一周酸; 和（iv）在所选溶剂下混合60-90重量％的生物吸收性聚合物和10-40重量％的牙齿磷灰石以制备陶瓷 - 聚合物复合材料。
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公开(公告)号：KR100393469B1
公开(公告)日：2003-08-06
申请号：KR1019990000031
申请日：1999-01-02
Applicant: 정필훈
Inventor： 정필훈
Abstract: PURPOSE: A making method of substitute using animal bones is provided, which can replace damaged bone part of the human body into the new substitute without immune rejection, also reinforces the bones to use as a bone pin, a bone screw and a bone fixing plate. CONSTITUTION: A bone substitute producing method includes the steps of collecting bones by size and part from young healthy animal having no disease after examinations and cleaning, treating the bones in hydrogen peroxide vapor for over 15minutes, in 100% of alcohol or solution of mixing chloroform and methanol more than 24hours, in 0.6N of hydrochloric acid for 6-12hours according to needed parts and situations, treating the bones with Phosphate buffer solution over 30minutes, keeping the bones at -70°C, performing lyophilization more than 24hours at a minimum, sterilizing by using electric beam, EO gas or 25KGy of irradiation, and immersing the bones resulted in a solution with antibiotics before use. Treating bones in hydrogen peroxide for 15minutes and processing lyophilization more than 24hours are the ways of solidifying the bones.
Abstract translation: 目的：提供一种用动物骨替代的制作方法，它可以将受损的人体骨头部分替换成没有免疫排斥反应的新替代物，还可以加强骨头作为骨钉，骨螺钉和骨固定板 。 一种骨替代物生产方法，包括以下步骤：在检查和清洁后，按规模和部分从未患病的年轻健康动物采集骨头，在过氧化氢蒸气中处理骨骼超过15分钟，在100％酒精或混合氯仿 甲醇超过24小时，0.6N盐酸中6-12小时，根据所需的部位和情况，用磷酸盐缓冲液处理30分钟以上的骨头，保持骨骼在-70和摄氏度，冻干超过24小时 最低限度，使用电子束，EO气体或25KGy照射进行灭菌，并且在使用前浸入骨头产生含有抗生素的溶液。 在过氧化氢中处理骨骼15分钟并且处理冻干超过24小时是固化骨骼的方式。
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公开(公告)号：KR1020020014428A
公开(公告)日：2002-02-25
申请号：KR1020000047706
申请日：2000-08-18
Applicant: 정필훈
IPC: C09B61/00
Abstract: PURPOSE: A natural dye for dyeing DNA and tissue cells and its dyeing method are provided, which is made by using the extract of the skin of wild grapes, is nontoxic and can dye DNA and tissue cells without using a fixing agent. CONSTITUTION: The natural dye for dying DNA comprises the extract of the skin of wild grapes; and a DNA dyeing dye. The natural dye for dying tissue comprises the extract of the skin of wild grapes; and a tissue dyeing dye. Preferably the extract is obtained by centrifuging the skin of wild grapes; and separating the supernatant. DNA or tissue is dyed by using the natural dye for dying DNA or the natural dye for dying tissue, without using a fixing agent. The tissue is one of cell substrate, lysosome, immunocyte, a plasma cell, lymphocyte or macrophage.
Abstract translation: 目的：提供用于染色DNA和组织细胞的天然染料及其染色方法，其通过使用野生葡萄皮肤的提取物而制成，是无毒的并且可以染色DNA和组织细胞而不使用固定剂。 构成：染色DNA的天然染料包括野生葡萄皮肤的提取物; 和DNA染色剂。 用于染色组织的天然染料包括野生葡萄皮肤的提取物; 和组织染色染料。 优选地，通过离心野生葡萄的皮肤获得提取物; 并分离上清液。 通过使用天然染料染色DNA或染色染色DNA或天然染料以染色组织，而不使用固色剂。 组织是细胞底物，溶酶体，免疫细胞，浆细胞，淋巴细胞或巨噬细胞之一。
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公开(公告)号：KR1020060039309A
公开(公告)日：2006-05-08
申请号：KR1020040088447
申请日：2004-11-02
Applicant: 정필훈
Inventor： 정필훈
IPC: A63B55/00
CPC classification number: A63B55/00 , A63B55/408 , A63B2210/50
Abstract: 본 발명은 골프채를 휴대 및 보관하도록하는 골프백에 관한 것으로서,
통형구조를 갖는 몸체와,
상기 몸체의 형상을 유지시켜주기 위한 프레임과,
상기 프레임에 골프백에 내부로 삽입되는 골프채를 각각 구분하기 위한 삽입관과,
상기 삽입관의 일부분이 프레임과 분리되는 소형백을 포함한 구성함을 특징으로 하는 분리형 골프백을 제공하는 뛰어난 효과가 있다.
골프백, 분리식 골프백,골프채,-
Patent Agency Ranking
公开(公告)号：KR1020060002484A
公开(公告)日：2006-01-09
申请号：KR1020040051531
申请日：2004-07-02
Applicant: 정필훈
Inventor： 정필훈
CPC classification number: G04B45/0038 , G04B45/0076
Abstract: 본 발명은 진자구동체를 이용한 동적 심미감을 갖는 모형 장식물 작동구조를 갖는 시계에 관한 것으로서, 특히 배터리가 내장된 시계용 무브먼트 케이싱 내부에 힌지축을 중점으로 회동하는 진자구동체를 내장하고, 상기 배터리로부터 전원을 공급받는 코일체에 의해 상기 진자구동체가 좌,우 회동되게 하며, 상기 진자구동체의 상,하단에는 절곡와이어를 갖는 별도의 작동구를 결합 형성하여, 그 진자구동체의 회동에 의해 작동구를 통해 동적 장식물이 연속적인 움직임을 갖게 함으로서,
하나의 케이싱 내부에 무브먼트와 진자구동체를 동시에 내장하여 하나의 배터리에 의해 동시 작동을 가능케 하므로 제조원가의 절감은 물론 생산 및 조립공정의 단순화를 꾀하여 생산원가의 절감이 가능하므로 매우 경제적이며, 상기 무브먼트와 근접된 위치에서 그 작동구가 구동되므로 무브먼트 중심의 안정된 설계가 가능함은 물론 상,하 작동구를 동시에 채택하거나 그 중 하나의 작동구만을 채택하여 다양한 형태의 동적 작동구를 갖는 시계의 설계가 가능하여 소비자로 하여금 선택의 폭을 넓게 한 진자구동체가 내장된 시계용 어셈블리에 대한 것이다.
시계, 어셈블리, 무브먼트, 진자구동체, 모형 장식물, 작동구-
公开(公告)号：KR100512383B1
公开(公告)日：2005-09-05
申请号：KR1020000047706
申请日：2000-08-18
Applicant: 정필훈
IPC: C09B61/00
Abstract: 머루로부터 껍질을 분리하여 교반하고, 소정 시간동안 실온에서 저장하여 원심분리로 머루껍질 액을 추출하고 이를 침전시켜서 상층 용액을 분리하여 제조되며, DNA 염색제 또는 생체조직 염색제로써 사용되는 것을 특징으로 하는 천연염색염료가 개시된다. 천연의 머루 껍질에서 추출한 염색 염료는 무독성이고 환경 친화적인 염색 염료일 뿐만 아니라, DNA을 비롯한 각종 조직을 모두 염색할 수 있다.
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公开(公告)号：KR1020050075739A
公开(公告)日：2005-07-21
申请号：KR1020050056779
申请日：2005-06-29
IPC: A61C8/00
CPC classification number: A61C8/0065 , A61C8/0022 , A61C8/0069 , A61C8/0078 , A61C8/0098
Abstract: 잇몸뼈 속에 박히는 고정체(Fixture)와 이와 결합하여 고정체를 팽창시켜 발치공에 고정하는 치아받침대(Abutment)로 구성되는 팽창형 임플란트가 개시된다.
인공치아를 지지하는 헤드(abutment head)와 테이퍼진 네크(neck) 및 나사몸체가 고정체 길이방향으로 일체로 이루어지는 치아받침대(abutment); 상기 네크와 동일하게 테이퍼져서 테이퍼 실링(taper sealing)이 되는 네크수납부가 상부에 형성되고, 상기 네크수납부에 연통되어 하단까지 연장되는 관통공이 내부에 형성되며, 적어도 하나 이상의 슬릿이 길이방향 일정위치로부터 하단까지 형성된 고정체(Fixture); 및 상기 고정체의 관통공 내부 직경보다는 큰 직경을 가지며 상기 관통공 내부에서 상기 나사몸체와 나사 결합되어 상기 나사몸체의 회전에 의해 이동하는 팽창너트를 포함한다.-
公开(公告)号：KR100450146B1
公开(公告)日：2004-09-30
申请号：KR1020020008789
申请日：2002-02-19
Applicant: 정필훈
Inventor： 정필훈
IPC: C07K1/14
CPC classification number: A61L27/36
Abstract: PURPOSE: A method for extracting tooth protein from an extracted tooth and the use thereof are provided, thereby easily and rapidly extracting the tooth protein. CONSTITUTION: A method for extracting tooth protein from an extracted tooth comprises the steps of: washing the extracted tooth with enamel with distilled water; pulverizing the tooth, washing the pulverized tooth, removing fat from the pulverized tooth using organic solvent, and removing lime from the pulverized tooth; and removing fat from the lime-removed tooth, washing the tooth and extracting tooth protein using 4M guanidine-HCl, wherein the extracted tooth protein is optionally subjected to electrophoresis and is optionally adsorbed to the PVDF membrane. The extracted tooth protein is adsorbed to chitosan.
Abstract translation: 目的：提供一种从提取的牙齿中提取牙齿蛋白质的方法及其用途，由此容易且迅速地提取牙齿蛋白质。 组成：一种从提取的牙齿中提取牙齿蛋白质的方法，包括以下步骤：用蒸馏水用釉质洗涤提取的牙齿; 粉碎牙齿，洗涤粉碎的牙齿，用有机溶剂从粉碎的牙齿中除去脂肪，并从粉碎的牙齿中除去石灰; 并从去除石灰的牙齿中去除脂肪，用4M盐酸胍洗涤牙齿并提取牙齿蛋白质，其中所提取的牙齿蛋白质任选地进行电泳并任选地吸附到PVDF膜上。 提取的牙齿蛋白被吸附到壳聚糖上。
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公开(公告)号：KR1020040065203A
公开(公告)日：2004-07-21
申请号：KR1020040051812
申请日：2004-07-03
Applicant: 정필훈
Inventor： 정필훈
CPC classification number: A61L27/3821 , A61K35/32 , A61L27/3683 , A61L2430/02
Abstract: PURPOSE: A method for extracting a tooth protein from a pulled tooth using PVDF membrane is provided to increase an extracting efficiency of a tooth protein and to confirm an amino acid sequence without a further step. CONSTITUTION: The method for extracting a tooth protein from a pulled tooth comprises the steps of: washing the whole tooth without removing an enamel from the pulled tooth, using a distilled water; crushing the tooth and washing the tooth; degreasing and deliming using an organic solvent; re-degreasing and washing the delimed specimen; and extracting the tooth protein. The tooth protein is used as a bone forming material or as a calcification and ossification material.
Abstract translation: 目的：提供一种使用PVDF膜从拉牙提取牙齿蛋白的方法，以提高牙齿蛋白质的提取效率，并进一步确认氨基酸序列。 构成：从拉牙提取牙齿蛋白质的方法包括以下步骤：使用蒸馏水清洗全牙齿而不从拔牙除去牙釉质; 粉碎牙齿并清洗牙齿; 使用有机溶剂进行脱脂和脱气; 重新脱脂和洗涤分离的样品; 并提取牙齿蛋白。 牙齿蛋白质用作骨形成材料或用作钙化和骨化材料。
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