Abstract:
본 발명은, 포밀 펩타이드 수용체(formyl peptide receptor, FPR) 또는 그의 유사 수용체에 작용하는 펩타이드를 호흡기 염증을 억제하는 데 효과적인 양으로 함유하는, 호흡기 염증성 질환의 치료 또는 예방을 위한 호흡기내 투여용 약학 제제, 상기 제제를 이용하여 호흡기 염증성 질환을 치료 또는 예방하는 방법 및 상기 제제를 포함하는 키트에 관한 것으로서, 상기 펩타이드를 주사로 전신에 투여하는 경우에 비해 직접 호흡기에 투여함으로써 호흡기 염증 억제 효과가 현저히 향상된다.
Abstract:
본발명은세포의지질막(cell membrane)에서유래된나노소포체(nanovesicles), 이의제조방법, 및상기나노소포체를이용한약제학적조성물, 진단키트등에관한것이다. 본발명에따른세포지질막유래나노소포체는치료용또는진단용물질전달에불필요한세포내 물질(유전물질및 세포내 단백질)이제거되어잠재적부작용을없앨수 있고, 세포또는조직타겟팅이가능하므로목표한세포또는조직에주로치료용또는진단용물질을전달하고다른곳에치료용또는진단용물질의전달을억제함으로써약물등 치료용물질의부작용을줄여서질병치료과정에서환자의고통과불편을줄일수 있으며, 치료효능을증가시킬수 있다. 또한, 질병치료용또는진단용물질이로딩된본 발명의세포지질막유래나노소포체및 그의제조방법은 in vitro 또는 in vivo에서치료또는진단용, 또는실험용으로사용될수 있다.
Abstract:
PURPOSE: A pharmaceutical composition containing nanovesicles derived from tumor tissue and a method for treating cancer using the same are provided to enable shape changing of a nanovesicle antigen into various forms and to develop a cancer therapeutic vaccine. CONSTITUTION: A pharmaceutical composition for treating cancer contains nanovesicles derived from a tumor tissue. The tumor tissue is autologously derived from a patient with tumor or is prepared by transformation to express heat shock protein. The composition additionally contains an immunoadjuvant, polyI:C. The membrane of the nanovesicle contains cyclodextrin or polyethylene glycol. A method for preparing the nanovesicle comprises: a step of separating cells from the tumor tissue; a step of performing extrusion, sonication, cell lysis, homogenization, freezing-thawing, electroporation, mechanical lysis, or chemical treatment of a tumor cell suspension and preparing nanovesicles; a step of separating the nanovesicles from the suspension; and a step of adding the immunoadjuvant and culturing. A cancer vaccine contains a nanovesicle antigen derived from the tumor tissue.
Abstract:
PURPOSE: A method for diagnosing inflammatory diseases and a diagnosis kit are provided to improve diagnosis accuracy. CONSTITUTION: A method for diagnosing inflammatory diseases comprises: a step of isolating cell adhesion proteins in fluid into water soluble protein fraction and extracellular endoplasmic reticulum fraction; and a step of quantitating proteins in the water soluble protein fraction and extracellular endoplasmic reticulum fraction. The proteins contain ICAM-1(intercellular adhesion molecule-1), E-selectin, and VCAM-1(vascular cell adhesion molecule-1). A method for diagnosing inflammatory diseases comprises a step of measuring the expression level of gene existing in the extracellular endoplasmic reticulum of body fluid. The cell adhesion protein is ICAM-1, E-selectin, and VCAM-1. [Reference numerals] (AA,EE) Cell culture liquid; (BB) Ultracentrifugation; (CC,FF) Soluble protein; (DD,GG) Extracellular endoplasmic reticulum
Abstract:
본 발명은 간세포에서 유래한 마이크로베시클(microvesicle)을 배아줄기세포에 처리하여 간세포로 분화시키는 방법에 관한 것으로서, 본 발명의 마이크로베시클은 간세포에 특이적인 단백질 및 mRNA 등이 포함되어 있으므로, 벡터를 이용한 다른 유전자를 삽입할 필요 없이 세포막으로 보호된 세포질만을 이용함으로써, 더욱 간편하면서도 효율적으로 간세포로의 분화가 일어나게 할 수 있는 유용한 효과가 있다.
Abstract:
The present invention relates to an immunity reinforcing composition which contains recombined fibrobalst growth factor-2 (FGF2) as an active ingredient and is capable of containing antigens. The composition of the present invention can be used as a pharmaceutical composition and a vaccine composition for preventing or treating: infectious diseases caused by virus, bacteria, fungi, or protozoa; or inflammation diseases caused by extracellular endoplasmic reticulum secreted from the bacteria, fungi, or protozoa.
Abstract:
PURPOSE: A method for preparing induced pluripotent stem cells by treating a composition containing a microvesicle is provided to develop cell therapeutic agent. CONSTITUTION: A method for preparing induced pluripotent stem cells comprises a step of treating somatic cells with a composition containing embryonic stem cell-derived microvesicle. The embryonic stem cells are derived from human or non-human primates, mouse, rat, dog, cat, house, or horse. The microvesicle additionally contains components except for a cell membrane of embryonic stem cells. The components are cyclodextrin or polyethylene glycol. The composition additionally contains a component except for the microvesicle, such as BFA(brefeldin A), BIX(BiP inducer X), or VPA(valproic acid). A cell therapeutic agent contains the induced pluripotent stem cells. [Reference numerals] (AA) Embryonic stem cell; (BB) Microvesicle