Abstract:
본명세서에는이산화탄소로부터말라리아치료전구물질인아모파디엔을생산할수 있는형질전환된시네코코커스일롱게투스균주가개시된다. 상기균주는이산화탄소를탄소원으로사용하여아르테미시닌의전구체인아모파디엔을대량생산할수 있다. 또한, 상기균주는, 빛과대기중에존재하는이산화탄소를탄소원으로사용하여고가의아르테미시닌의전구체인아모파디엔을생산하기때문에경제적이며, 미생물을사용하여, 대기중의이산화탄소를제거또는저감하는데활용될수 있으므로, 친환경적이다. 또한, 균주배양에기후의영향을받지않기때문에안정적으로아모파디엔을생산할수 있는이점이있다.
Abstract:
본명세서에는시아노박테리아및 이콜라이에서모두사용할수 있는벡터로서순서대로, 복제개시점으로서 pUC 복제개시점; 선별마커로서스펙티노마이신(spectinomycin) 저항성유전자; 및 trc 프로모터, tetA 프로모터또는변형된 tetA 프로모터, BAD 프로모터, 및 cbbL 프로모터로구성된군으로부터선택된프로모터를포함하는벡터가개시된다. 상기벡터로형질전환된숙주세포를이용하여산업적으로유용한물질을효과적으로생산할수 있다. 또한, 상기벡터를이용하면다양한타겟유전자를손쉽게조합하여삽입할수 있고, 이를통해다양한벡터를효율적으로제조할수 있다.
Abstract:
본발명에의한푸르푸랄내성유전자를포함하는푸르푸랄내성균주는푸르푸랄이포함되어있는배지에서효과적인생장이가능하다. 따라서비식용바이오매스인목질계바이오매스로부터유래한당화액등에푸르푸랄과같은독성부산물이포함되어미생물의발효가어려웠던문제를해결할수 있다. 또한, 본발명에의한균주의제조방법에의하면비교적적은수의타겟유전자들을가지고내성유전자를선별할수 있어서내성균주개발을위한시간과비용등을절약할수 있다. 또한이러한유전자발굴방법은상기푸르푸랄내성유전자외에알려져있지않은다른여러가지기능성유전자를찾아내는방법에널리적용될수 있다.
Abstract:
The present statement provides a step of proceeding tyrosinase in lignin and making the mixture react, as a method for degrading a catalyst with lignin. When using the method, tyrosinase dose not need an alternate group, lignin is effectively degraded due to stability and activity, in a more eco-friendly way then heat or oxidation treatment, and lignin is also effectively degraded from ligro-cellulosic biomass, leading to easy production of bio fuel.
Abstract:
PURPOSE: A method for preparing butanone from levulinic acid using a decarboxylase biocatalyst is provided to produce butanone which is a biofuel for various applications by enzymatic decarboxylation of levulinic acid under the presence of a biocatalyst. CONSTITUTION: A method for preparing butanone comprises the step of performing decarboxylization of levulinic acid under the presence of an acetoacetate decarboxylase (AADC) biocatalyst, an AADC expressing microorganism, or a mixture thereof and an electron transport mediator. The electron transport mediator is methyl viologen, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), or a mixture thereof. The AACD biocatalyst is prepared from Clostridium sp., Psudomonas sp., Chromobacterium sp., or Paenibacillus sp. [Reference numerals] (AA) Relative activity(%); (BB) Concentration of a substrate(mM)
Abstract:
PURPOSE: A method for preparing hexanoic acid using in situ extractive fermentation is provided to produce and easily collect hexanoic acid of high yield using sucrose as a carbon source. CONSTITUTION: A method for preparing hexanoic acid using a microorganism comprises the steps of: inoculating hexanoic acid-producing strains to a culture medium and culturing; adding an organic solvent to the culture medium; continuously culturing the strains; and removing the organic solvents and obtaining hexanoic acid.
Abstract:
PURPOSE: A butyric acid producing method using a strain from Clostridium is provided to effectively produce butyric acid from galactan, a marine algae biomass, having a main carbohydrate component by producing butyric acid form galactose. CONSTITUTION: A strain from Clostridium uses galactose to produce butyric acid. A butyric acid producing method from galactose comprises treating the strain from the Clostridium on galactose and culturing to convert galactose to butyric acid. A separating method of the strain from the Clostridium comprises a step of removing a gram-negative bacteria by heat treating an anaerobic sludge, a step of inoculating the heat treated anaerobic sludge to the culture medium containing galactose and culturing, a step of selecting colonies with butyric acid resistancy by smearing the culturing solution obtained from the culturing to the culture medium containing butyric acid or the salt, and a step of separating the most butyric acid producing strain by measuring butyric acid of the selected colonies.