이산화탄소 고정능을 가지는 재조합 미생물 및 이를 이용한 유용물질의 제조 방법
    22.
    发明公开
    이산화탄소 고정능을 가지는 재조합 미생물 및 이를 이용한 유용물질의 제조 방법 审中-实审
    具有二氧化碳固化能力的重组微生物及其生产有用物质的方法

    公开(公告)号:KR1020130055048A

    公开(公告)日:2013-05-28

    申请号:KR1020110119420

    申请日:2011-11-16

    CPC classification number: C12N9/93

    Abstract: PURPOSE: A recombinant microorganism containing a gene encoding an ACS/CODH(acetyl-CoA synthases/carbon monoxide dehydrogenase) complex is provided to ensure carbon dioxide fixation ability, and to produce high concentratioin and high yield useful substances such as alcohol and organic acid. CONSTITUTION: A recombinant microorganism with a carbon dioxide fixation ability contains a gene encoding ACS/CODH complex or a recombinant vector containing the gene in a host microorganism. A method for preparing the recombinant microorganism comprises a step of introducing the gene or the recombinant vector into the host microorganism. The method for fixing carbon dioxide comprises a step of culturing the recombinant microorganism under the presence of CO_2. A method for preparing the useful substance comprises: a step of culturing the recombinant microorganism using CO_2 as a carbon source; and a step of generating and collecting the useful substance. The useful substance is alcohol including butanol or ethanol.

    Abstract translation: 目的:提供含有编码ACS / CODH(乙酰辅酶A合成酶/一氧化碳脱氢酶)基因的基因的重组微生物,以确保二氧化碳固定能力,并产生高浓度和高产量的有用物质如酒精和有机酸。 构成:具有二氧化碳固定能力的重组微生物包含编码ACS / CODH复合物的基因或在宿主微生物中含有该基因的重组载体。 制备重组微生物的方法包括将基因或重组载体导入宿主微生物的步骤。 固定二氧化碳的方法包括在CO_2存在下培养重组微生物的步骤。 制备有用物质的方法包括:使用CO 2作为碳源培养重组微生物的步骤; 以及生成和收集有用物质的步骤。 有用的物质是醇,包括丁醇或乙醇。

    랄스토니아 속 미생물의 유전자를 불활성화시키는 방법
    23.
    发明公开
    랄스토니아 속 미생물의 유전자를 불활성화시키는 방법 无效
    在基因突变体中灭活目标基因的方法

    公开(公告)号:KR1020110122434A

    公开(公告)日:2011-11-10

    申请号:KR1020100041955

    申请日:2010-05-04

    Abstract: PURPOSE: A method for inactivating Ralstonia sp. microorganism gene is provided to enable genetic study of the microorganism. CONSTITUTION: A recombinant vector contains: sacB gene encoding evansucrase of Bacillus subtilis, a selective marker, a base sequence for selective marker removal; and a sequence for homology recombination. The recombinant vector additionally contains origin of replication. The recombinant vector additionally contains a gene which enables DNA transition by hybridization between cells. The selective marker exists between the sequences causing homology recombination with a target gene.

    Abstract translation: 目的:灭活Ralstonia sp。 提供微生物基因以实现微生物的遗传研究。 构成:重组载体含有:编码枯草芽孢杆菌的evansucrase的sacB基因,选择性标记,用于选择性标记去除的碱基序列; 和同源重组序列。 重组载体另外含有复制起点。 重组载体另外含有通过细胞之间的杂交使DNA转换的基因。 在引起与靶基因的同源重组的序列之间存在选择性标记。

    거대 플라스미드의 결실에 의해 목적물질의 생성능이 저하되는 것을 방지할 수 있는 변이 미생물 및 그 제조방법
    24.
    发明公开
    거대 플라스미드의 결실에 의해 목적물질의 생성능이 저하되는 것을 방지할 수 있는 변이 미생물 및 그 제조방법 无效
    通过保护微胶囊失活及其微生物制备非变性微生物的制备方法

    公开(公告)号:KR1020110032376A

    公开(公告)日:2011-03-30

    申请号:KR1020090089828

    申请日:2009-09-22

    Abstract: PURPOSE: A mutant microorganism which prevents productivity reduction of a target material is provided to prevent degeneration of the microorganism regardless of medium and fermentation condition. CONSTITUTION: A mutant microorganism which prevents productivity reduction of a target material by megaplasmid deletion is prepared by a part of inserting essential chromosome DNA and complementary site to a microorganism with the megaplasmid. The part of the chromosome DNA is a gene encoding DNA polymerase, RNA polymerase, or ribosome.

    Abstract translation: 目的:提供一种防止目标材料生产力下降的突变体微生物,以防止微生物的退化,而与培养基和发酵条件无关。 构成:通过将重要的染色体DNA和互补位点插入到具有大质粒的微生物的一部分来制备通过大质粒缺失来防止目标材料的生产力降低的突变微生物。 染色体DNA的一部分是编码DNA聚合酶,RNA聚合酶或核糖体的基因。

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