Abstract:
PURPOSE: Conjugates comprising oligonucleotide and hydrophilic polymers for gene transfer, hybrid polyion complex micelles and preparation methods thereof are provided, thereby effectively transferring the oligonucleotide within a cell, and exhibiting the activity of antisense oligonucleotide at relatively low concentration. CONSTITUTION: Conjugates comprising oligonucleotide and hydrophilic polymers for gene transfer are provided, wherein the terminal group of the oligonucleotide is covalently coupled to the hydrophilic polymers; the hydrophilic polymer is selected from nonionic polymers having the molecular weight of 500; the molecular weight of oligonucleotide is selected from between 1,000 to 50,000; the hydrophilic polymer is selected from polyethyleneglycol, polyvinylpyrrolidone, and polyoxazoline; the bond between oligonucleotide and hydrophilic polymer is selected from amide bond, carbamate bond, hydrazone bond, phosphoroamidate bond, acetal bond, disulfide bond, ester bond, and anhydride bond; and the antisense oligonucleotide comprises a portion or the total of c-myc, c-myb, c-fos, or c-jun.
Abstract:
본 발명은 밀봉 성능을 높인 고체산화물 연료전지용 단위셀, 이를 포함하는 고체산화물 연료전지 및 그 제조 방법에 관한 것으로서, 더욱 상세하게 경사부가 형성된 연료극을 이용하여 하측 면을 제외한 전 영역 또는 금속지지체의 상면 영역까지 얇은 전해질층을 용이하게 형성할 수 있어 실링성을 향상할 수 있으며, 제조 효율을 높일 수 있는 밀봉 성능을 높인 고체산화물 연료전지용 단위셀, 이를 포함하는 고체산화물 연료전지 및 그 제조 방법에 관한 것이다.
Abstract:
PURPOSE: A manufacturing method of a solid oxide fuel cell is provided to preserve pores formed on a metal foam support during process, thereby manufacturing a solid oxide fuel having excellent strength and flexibility. CONSTITUTION: A manufacturing method of a solid oxide fuel cell comprises: a first step of injecting complementary material(22) into pores(21) of metal foam support(20); a second step for forming an adhesive layer on the metal foam support; a step of laminating fuel electrode(40) on the adhesive layer; and a step of sintering the adhesive layer for contacting the metal foam support and the fuel electrode. The manufacturing method additionally comprises a step of removing the complementary material after the second step. A solid oxide fuel cell comprises a metal foam support in which pores are formed, and a single cell with a fuel electrode.
Abstract:
PURPOSE: A unit cells-fixed fuel cell using a sealing material is provided to firmly fix the metal plate and the cell and to prevent the leakage of supplying fuel by air-tightening a gap between a metal plate and the cell. CONSTITUTION: A unit cells-fixed fuel cell includes unit cells(30) with fuel electrodes, a metal plate(10), and a sealing material(40). The metal plate includes a receiving part(16), a loading part(15), and a groove(14). The receiving part receives the unit cells. The loading part forms the bottom of the receiving part, and the unit cells are loaded on the loading part. The groove is arranged between sidewalls and the loading part. The sealing material is arranged between the unit cells and the metal plate to fix the unit cells to the metal plate. A gap(18) is arranged between the metal plate and sidewalls. The sealing material is filled in the groove through the gap.
Abstract:
PURPOSE: A fuel cell is provided to measure temperature in stack by inserting a plurality of thermocouple in each cell, thereby real time controlling the supply and the composition of fuel gas when operating. CONSTITUTION: A manufacturing method of a fuel cell including thermo-couple comprises: a step forming insulating layer(11) on the upper part of the electrolyte layer(10); a step forming electrode(13) functioning the hot junction of thermo-couple in upper part of the insulating layer, and forming lead wire(12) for applying electric current with the electrode; a step expanding the insulating layer to cover the electrode and the lead wire by using material which is same to the material of the insulating layer; a step forming a fuel electrode(14) on the insulating layer; and a step forming air electrode(15) under the electrolyte layer.
Abstract:
PURPOSE: A method for manufacturing a solid oxide fuel cell is provided to prevent the loss by the damage caused by mechanical strength and impact and to obtain a solid oxide fuel cell in which the weight is reduced. CONSTITUTION: A solid oxide fuel cell comprises a metal foam supporter(20) in which pores(21) are formed and a unit cell including a fuel electrode(40). A method for manufacturing a solid oxide fuel cell comprises the steps of: flowing in a complementary material(22) in the pores of the metal foam supporter; forming an adhesive layer to the metal foam supporter; laminating the fuel electrode of the unit cell to the adhesive layer; and sintering the adhesive layer in order to mutually contact the metal foam supporter and the fuel electrode.
Abstract:
PURPOSE: A process for preparing mixed micelles containing oligonucleotide and aliphatic polyester-based polymers is provided. Whereby, the mixed micelles can effectively deliver the oligonucleotide, which is used in gene therapy, into cells and the release amount thereof can be easily controlled. CONSTITUTION: An aliphatic polyester-based biodegradable polymer and an activating agent react. The reaction product is dried, mixed with oligonucleotide containing an amine group at the 5'-terminus in a mole ratio of 2:1 to 1:2 and then reacts in an organic solvent to produce a conjugate. The conjugate is dispersed in distilled water to produce a micelle. The aliphatic polyester-based biodegradable polymer is polylactic acid, polyglycolic acid, poly(D,L-lactic acid-co-glycolic acid), polycaprolactone, polyvalero-lactone, polyhydroxy butylate, polyhydroxy valerate or a mixture thereof.