公开(公告)号：AU2250497A

公开(公告)日：1997-08-22

申请号：AU2250497

申请日：1997-01-31

Applicant: AURORA BIOSCIENCES CORP ,  UNIV CALIFORNIA

Inventor： TSIEN ROGER Y ,  HEIM ROGER ,  CUBITT ANDREW

IPC: C07K14/435 ,  C12N1/21 ,  C12N15/12 ,  C12Q1/37 ,  C12Q1/48 ,  G01N33/542 ,  C12N15/62 ,  C12N15/70 ,  C12N15/85 ,  C12N5/10 ,  G01N33/553

Abstract: This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.

公开(公告)号：CA2245108A1

公开(公告)日：1997-08-07

申请号：CA2245108

申请日：1997-01-31

Applicant: UNIV CALIFORNIA ,  AURORA BIOSCIENCES CORP

Inventor： TSIEN ROGER Y ,  HEIM ROGER ,  CUBITT ANDREW

IPC: C07K14/435 ,  C12N1/21 ,  C12N15/12 ,  C12Q1/37 ,  C12Q1/48 ,  G01N33/542 ,  C12N15/62 ,  C07K19/00 ,  C12Q1/34 ,  C12N15/65

Abstract: This invention provides tandem fluorescent protein constructs including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.

公开(公告)号：AU2004200425A8

公开(公告)日：2004-03-04

申请号：AU2004200425

申请日：2004-02-05

Applicant: AURORA BIOSCIENCES CORP ,  STATE OF OREGON ACTING BY AND THROUGH THE STATE BOARD OF HIGHER EDUCATION ON BEHALF OF THE UNIVERSIT ,  UNIV CALIFORNIA

Inventor： TSIEN ROGER Y ,  REMINGTON JAMES S ,  ORMO MATS F ,  HEIM ROGER ,  CUBITT ANDREW B

IPC: C07H21/04 ,  C07K14/195 ,  C07K16/12 ,  C07K16/44 ,  C12N15/63

公开(公告)号：AU767375B2

公开(公告)日：2003-11-06

申请号：AU2319601

申请日：2001-02-23

Applicant: UNIV CALIFORNIA ,  OREGON STATE ,  AURORA BIOSCIENCES CORP

Inventor： TSIEN ROGER Y ,  CUBITT ANDREW B ,  HEIM ROGER ,  ORMO MATS F ,  REMINGTON JAMES S

IPC: C07H21/04 ,  C07K14/195 ,  C07K16/12 ,  C07K16/44 ,  C12N15/63

公开(公告)号：CA2262739C

公开(公告)日：2003-11-04

申请号：CA2262739

申请日：1998-05-29

Applicant: AURORA BIOSCIENCES CORP

Inventor： PHAM ANDREW A ,  HAROOTUNIAN ALEC TATE ,  COASSIN PETER J ,  MENDLEIN JOHN D ,  TSIEN ROGER Y

IPC: G01N21/64 ,  B01L3/00 ,  B01L9/00 ,  C08G61/08 ,  C40B60/14 ,  G01N21/03 ,  G01N33/483 ,  G01N33/52

Abstract: The invention provides for multi-well plates and platforms that comprise a layer of having low fluorescence and high transmittance. These multi-well plates and platforms are particularly well suited for fluorescent measuremen ts.

公开(公告)号：AT218615T

公开(公告)日：2002-06-15

申请号：AT97905667

申请日：1997-01-31

Applicant: UNIV CALIFORNIA ,  AURORA BIOSCIENCES CORP

Inventor： TSIEN ROGER Y ,  HEIM ROGER ,  CUBITT ANDREW

IPC: C07K14/435 ,  C12N1/21 ,  C12N15/12 ,  C12Q1/37 ,  C12Q1/48 ,  G01N33/542 ,  C12N15/62 ,  C12N15/70 ,  C12N15/85 ,  C12N5/10

Abstract: This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.

公开(公告)号：AU717873B2

公开(公告)日：2000-04-06

申请号：AU2250497

申请日：1997-01-31

Applicant: UNIV CALIFORNIA ,  AURORA BIOSCIENCES CORP

Inventor： TSIEN ROGER Y ,  HEIM ROGER ,  CUBITT ANDREW

IPC: C07K14/435 ,  C12N1/21 ,  C12N15/12 ,  C12Q1/37 ,  C12Q1/48 ,  G01N33/542 ,  C12N15/62 ,  C12N15/70 ,  C12N15/85 ,  C12N5/10 ,  G01N33/553

Abstract: This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.