公开(公告)号：WO2005059093A3

公开(公告)日：2006-03-02

申请号：PCT/EP2004014266

申请日：2004-12-15

Applicant: BASF AG ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C07K14/34 ,  C12N20060101 ,  C12N1/00 ,  C12N15/63 ,  C12N15/67 ,  C12N15/77 ,  C12P1/00 ,  C12P13/08 ,  C12P13/12 ,  C12N1/21

CPC classification number: C07K14/34 ,  C12N15/77 ,  C12P13/08 ,  C12P13/12

Abstract: The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the gene transcription rate and/or expression rate, expression cassettes containing said expression units, genetically modified microorganisms having a modified or induced transcription rate and/or expression rate, and methods for producing biosynthetic products by cultivating said genetically modified microorganisms.

Abstract translation: 本发明涉及使用核酸序列的调节基因，所述新的启动子和表达单元本身的转录和表达，用于改变或引起转录和/或基因的表达率的速率的方法，含有该表达单元的表达盒，具有改变的遗传修饰的微生物或 引起转录速率和/或表达的速率以及通过培养遗传修饰的微生物的制备生物合成产物的方法。

公开(公告)号：WO2005059139A3

公开(公告)日：2005-08-11

申请号：PCT/IB2004004429

申请日：2004-12-17

Applicant: BASF AG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N9/16 ,  C12P13/08 ,  C12N15/52

CPC classification number: C12P13/08 ,  C12N9/16

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose- l,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-­bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

Abstract translation: 本发明的特征在于通过使酶编码基因（即果糖-1,6-二磷酸酶）失调来增加精细化学品例如来自微生物例如棒状杆菌的赖氨酸的生产方法。 在优选的实施方案中，本发明提供了通过增加果糖-1,6-二磷酸酶活性的表达来增加谷氨酸棒杆菌中赖氨酸的产生的方法。 本发明还提供了通过调节向草酰乙酸（OAA）的碳通量来生产赖氨酸的新方法。 在优选的实施方案中，本发明提供了通过使用果糖或蔗糖作为碳源来生产赖氨酸的方法。

公开(公告)号：WO2004024932A3

公开(公告)日：2004-04-22

申请号：PCT/EP0309452

申请日：2003-08-26

Applicant: BASF AG ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: A23K1/00 ,  A23K1/16 ,  C12N15/09 ,  C12P13/04 ,  C12P13/12

CPC classification number: C12P13/04 ,  C12P13/12

Abstract: The invention relates to methods for the zymotic production of fine chemicals, especially L-methionine, containing sulphur using bacteria, wherein a nucleotide sequence coding for a methionine-synthase (methA)-gene is expressed.

Abstract translation: 本发明涉及使用其中表达编码甲硫氨酸合酶（methA）基因核苷酸序列的细菌来发酵生产含硫精细化学品，特别是L-甲硫氨酸的方法。

公开(公告)号：WO03040289A2

公开(公告)日：2003-05-15

申请号：PCT/EP0212132

申请日：2002-10-31

Applicant: BASF AG ,  ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N1/21 ,  C12N15/52 ,  C12P13/04 ,  C12N

CPC classification number: C12P13/04 ,  C12N15/52

Abstract: The invention concerns novel nucleic acid molecules, the use of said molecules for producing by recombination genetically improved micro-organisms, and a method for preparing fine chemical products, in particular amino acids, using said genetically improved micro-organisms.

Abstract translation: 本发明涉及新的核酸分子，它们的遗传改良的微生物以及用于产生精细化学品，在使用这些遗传改良的微生物的特定氨基酸的方法的结构中使用。

公开(公告)号：WO02070685A2

公开(公告)日：2002-09-12

申请号：PCT/EP0202133

申请日：2002-02-28

Applicant: BASF AG ,  POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12N15/77 ,  C12R1/13 ,  C12N15/00

CPC classification number: C12N9/1055 ,  C12N15/77

Abstract: The invention relates to a novel method of modifying the genome of gram-positive bacteria, to said bacteria and to novel vectors. The invention especially relates to a method of modifying Corynebacteria or Brevibacteria using a novel marker gene that has a conditionally negative dominant effect.

Abstract translation: 本发明涉及一种新颖的方法，用于改变革兰氏阳性菌，这些细菌和新载体的基因组中。 特别地，本发明涉及使用新的条件占优势的细菌标志基因负性的改变棒杆菌或Brevibacterien的方法。

公开(公告)号：WO0100844A3

公开(公告)日：2001-07-19

申请号：PCT/IB0000943

申请日：2000-06-23

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: G01N33/53 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/12 ,  C12N9/18 ,  C12N9/88 ,  C12N15/09 ,  C12N15/31 ,  C12N15/55 ,  C12P20060101 ,  C12P13/04 ,  C12P13/08 ,  C12P21/02 ,  C12Q20060101 ,  C12Q1/68 ,  C12R1/15 ,  G01N33/566 ,  G01N33/569

CPC classification number: C12N9/88 ,  C07K14/34 ,  C12N9/16 ,  C12N9/18

Abstract: Isolated nucleic acid molecules, designated SMP nucleic acid molecules, which encode novel SMP proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SMP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SMP proteins, mutated SMP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SMP genes in this organism.

Abstract translation: 描述了分离的核酸分子，称为SMP核酸分子，其编码来自谷氨酸棒状杆菌的新型SMP蛋白质。 本发明还提供了反义核酸分子，含有SMP核酸分子的重组表达载体以及导入了表达载体的宿主细胞。 本发明还进一步提供了分离的SMP蛋白，突变的SMP蛋白，融合蛋白，抗原肽和用于改善从C产生所需化合物的方法。 基于该生物体中SMP基因的遗传工程改造的谷氨酸棒杆菌。

公开(公告)号：WO2006008097A2

公开(公告)日：2006-01-26

申请号：PCT/EP2005007752

申请日：2005-07-16

Applicant: BASF AG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C12N15/00

CPC classification number: C12N15/63 ,  C07K14/34 ,  C12N15/77 ,  C12P13/04

Abstract: The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the transcription rate and/or expression rate of genes, expression cassettes containing said expression units, genetically modified microorganisms with a modified or induced transcription rate and/or expression rate, and methods for producing biosynthetic products by cultivating the genetically modified microorganisms.

Abstract translation: 本发明涉及使用核酸序列的调节基因，所述新的启动子和表达单元本身的转录和表达，用于改变或引起转录和/或基因的表达率的速率的方法，含有该表达单元的表达盒，具有改变的遗传修饰的微生物或 引起转录速率和/或表达的速率以及通过培养遗传修饰的微生物的制备生物合成产物的方法。

公开(公告)号：WO2005059154A3

公开(公告)日：2005-10-13

申请号：PCT/IB2004004426

申请日：2004-12-17

Applicant: BASF AG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N9/04 ,  C12P13/08 ,  C12N1/21 ,  C12N15/53

CPC classification number: C12N9/0006 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., lactate dehydrogenase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of the expression of lactate dehydrogenase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

Abstract translation: 本发明的特征在于通过使酶编码基因（即乳酸脱氢酶）失调来增加精细化学品例如来自微生物例如棒状杆菌的赖氨酸的生产方法。 在优选的实施方案中，本发明提供通过乳酸脱氢酶活性的表达增加谷氨酸棒杆菌中赖氨酸的产生的方法。 本发明还提供了通过调节向草酰乙酸（OAA）的碳通量来生产赖氨酸的新方法。 在优选的实施方案中，本发明提供了通过使用果糖或蔗糖作为碳源来生产赖氨酸的方法。

公开(公告)号：WO2005058945A2

公开(公告)日：2005-06-30

申请号：PCT/EP2004014338

申请日：2004-12-16

Applicant: BASF AG ,  KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  RUFFER UWE ,  GRAEF CLAUDIA ISABELLA ,  HABERHAUER GREGOR

Inventor： KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  RUFFER UWE ,  GRAEF CLAUDIA ISABELLA ,  HABERHAUER GREGOR

IPC: C07K14/34 ,  C07K14/195

CPC classification number: C07K14/34

Abstract: The invention relates to mutant nucleic acids and proteins from the metabolic pathway of fine chemicals, methods for the production of genetically modified production organisms, methods for the production of fine chemicals by cultivating said genetically modified organisms, and said genetically modified organisms.

Abstract translation: 本发明涉及从精细化学品的途径突变型的核酸和蛋白质，通过培养转基因生物，以及经遗传修饰的生物体本身生产的遗传修饰的生物体的生产工艺，用于生产精细化学品的方法。

公开(公告)号：WO03100072A2

公开(公告)日：2003-12-04

申请号：PCT/EP0305423

申请日：2003-05-23

Applicant: BASF AG ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN

Inventor： SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12P13/12 ,  C12R1/15 ,  C12P11/00

CPC classification number: C12N9/1085 ,  C12P13/12 ,  C12R1/15

Abstract: The invention relates to methods for the production of sulphur-containing fine chemicals by fermentation, in particular L-methionine, using bacteria in which a nucleotide sequence is expressed which codes for an S-adenosylmethionine synthase (metK) gene.

Abstract translation: 本发明涉及一种用于发酵生产的含硫精细化学品，特别是LMethionin，利用细菌，其中S-腺苷甲硫氨酸合成酶（克隆metK）基因的核苷酸编码序列的表达的一个方法。