公开(公告)号：WO02070685A2

公开(公告)日：2002-09-12

申请号：PCT/EP0202133

申请日：2002-02-28

Applicant: BASF AG ,  POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12N15/77 ,  C12R1/13 ,  C12N15/00

CPC classification number: C12N9/1055 ,  C12N15/77

Abstract: The invention relates to a novel method of modifying the genome of gram-positive bacteria, to said bacteria and to novel vectors. The invention especially relates to a method of modifying Corynebacteria or Brevibacteria using a novel marker gene that has a conditionally negative dominant effect.

Abstract translation: 本发明涉及一种新颖的方法，用于改变革兰氏阳性菌，这些细菌和新载体的基因组中。 特别地，本发明涉及使用新的条件占优势的细菌标志基因负性的改变棒杆菌或Brevibacterien的方法。

公开(公告)号：WO0100844A3

公开(公告)日：2001-07-19

申请号：PCT/IB0000943

申请日：2000-06-23

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: G01N33/53 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/12 ,  C12N9/18 ,  C12N9/88 ,  C12N15/09 ,  C12N15/31 ,  C12N15/55 ,  C12P20060101 ,  C12P13/04 ,  C12P13/08 ,  C12P21/02 ,  C12Q20060101 ,  C12Q1/68 ,  C12R1/15 ,  G01N33/566 ,  G01N33/569

CPC classification number: C12N9/88 ,  C07K14/34 ,  C12N9/16 ,  C12N9/18

Abstract: Isolated nucleic acid molecules, designated SMP nucleic acid molecules, which encode novel SMP proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SMP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SMP proteins, mutated SMP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SMP genes in this organism.

Abstract translation: 描述了分离的核酸分子，称为SMP核酸分子，其编码来自谷氨酸棒状杆菌的新型SMP蛋白质。 本发明还提供了反义核酸分子，含有SMP核酸分子的重组表达载体以及导入了表达载体的宿主细胞。 本发明还进一步提供了分离的SMP蛋白，突变的SMP蛋白，融合蛋白，抗原肽和用于改善从C产生所需化合物的方法。 基于该生物体中SMP基因的遗传工程改造的谷氨酸棒杆菌。

公开(公告)号：WO2007011845A3

公开(公告)日：2007-04-12

申请号：PCT/US2006027617

申请日：2006-07-18

Applicant: BASF AG ,  ZELDER OSKAR ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  YOCUM R ROGERS ,  WILLIAMS MARK K

Inventor： ZELDER OSKAR ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  YOCUM R ROGERS ,  WILLIAMS MARK K

IPC: C12P13/12 ,  C07K14/32 ,  C07K14/34 ,  C12N5/10

CPC classification number: C12P13/12 ,  C07K14/32 ,  C07K14/34 ,  C12P3/00 ,  C12P5/023 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/28 ,  C12P7/48 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  C12P13/04 ,  C12P23/00 ,  Y02E50/10 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present invention pertains to improved microorganisms and methods for the production of methionine and other sulfur containing fine chemicals using the metl gene from Bacillus subtilis or a gene related to metI. In some embodiments of the present invention, the metI gene or another gene is integrated in a fashion that allows for co-production of a water soluble compound such as methionine or other amino acid and a caortenoid compound.

Abstract translation: 本发明涉及使用来自枯草芽孢杆菌的metl基因或与metI有关的基因来生产甲硫氨酸和其他含硫精细化学品的改良微生物和方法。 在本发明的一些实施方案中，metI基因或另一基因以允许共同产生水溶性化合物如甲硫氨酸或其他氨基酸和类胡萝卜素化合物的方式整合。

公开(公告)号：WO2004024931A3

公开(公告)日：2004-04-22

申请号：PCT/EP0309451

申请日：2003-08-26

Applicant: BASF AG ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: A23K1/00 ,  A23K1/16 ,  C12N15/09 ,  C12P13/04 ,  C12P13/12 ,  C12R1/15

CPC classification number: C12P13/04 ,  C12P13/12

Abstract: The invention relates to methods for the production by fermentation of sulphur-containing fine chemicals, in particular L-methionine, using bacteria in which a nucleic acid sequence coding for a methionine synthase gene (metF) is expressed.

Abstract translation: 本发明涉及一种用于发酵生产的含硫精细化学品的方法，特别是L-甲硫氨酸，利用细菌，其中用于甲硫氨酸合酶一个（metF）基因编码核苷酸序列被表达。

公开(公告)号：WO03040681A2

公开(公告)日：2003-05-15

申请号：PCT/EP0212141

申请日：2002-10-31

Applicant: BASF AG ,  ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N1/21 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  G01N

CPC classification number: C12N15/77 ,  C12N15/52 ,  C12P13/08

Abstract: The invention relates to novel nucleic acid molecules, the use thereof in the construction of bio-engineering improved microorganisms and methods for the production of fine chemicals, especially amino acids with the aid of said bio-engineered improved microorganisms.

Abstract translation: 本发明涉及新的核酸分子，它们的遗传改良的微生物以及用于产生精细化学品，在使用这些遗传改良的微生物的特定氨基酸的方法的结构中使用。

公开(公告)号：WO0224917A3

公开(公告)日：2002-06-27

申请号：PCT/EP0110805

申请日：2001-09-19

Applicant: BASF AG ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  ZELDER OSKAR

Inventor： POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  ZELDER OSKAR

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12N15/77 ,  C12P1/04 ,  C12P13/04 ,  C12P13/08 ,  C12P13/12 ,  C12P13/14 ,  C12N15/68

CPC classification number: C12N9/1007 ,  C12N15/77

Abstract: The invention relates to a method for producing corynebacteria that contain one or more modified genomic sequences, characterized by using a vector that does not replicate in corynebacteria and whose nucleic acid is not recognized as foreign by the corynebacteria.

Abstract translation: 本发明涉及含有一种或使用非复制在棒状杆菌载体用于制备棒状杆菌的方法，所述核酸不是由棒状杆菌识别为外来更多改变的基因组序列。

公开(公告)号：WO0102583A3

公开(公告)日：2001-07-26

申请号：PCT/IB0000973

申请日：2000-06-27

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: G01N33/53 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12N9/12 ,  C12N15/09 ,  C12N15/31 ,  C12N15/54 ,  C12P1/04 ,  C12P13/04 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/13 ,  C12R1/16 ,  G01N33/566 ,  G01N33/569 ,  C12N15/77

CPC classification number: C12N9/1223 ,  C12N9/12 ,  C12Y207/03009

Abstract: Isolated nucleic acid molecules, designated PTS nucleic acid molecules, which encode novel PTS proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PTS nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated PTS proteins, mutated PTS proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of PTS genes in this organism.

Abstract translation: 描述了编码来自谷氨酸棒杆菌的新型PTS蛋白质的分离的核酸分子，命名为PTS核酸分子。 本发明还提供反义核酸分子，含有PTS核酸分子的重组表达载体，以及引入了表达载体的宿主细胞。 本发明还进一步提供了基于该生物体中PTS基因的遗传工程的分离的PTS蛋白，突变的PTS蛋白，融合蛋白，抗原肽和用于从谷氨酸棒状病毒从谷氨酸棒球菌生产所需化合物的方法。

公开(公告)号：WO2007051725A3

公开(公告)日：2007-06-28

申请号：PCT/EP2006067680

申请日：2006-10-23

Applicant: BASF AG ,  ZELDER OSKAR ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH ,  KROEMER JENS ,  PERO JANICE G ,  YOCUM R ROGERS ,  PATTERSON THOMAS A ,  WILLIAMS MARK ,  HERMAN THERON

Inventor： ZELDER OSKAR ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH ,  KROEMER JENS ,  PERO JANICE G ,  YOCUM R ROGERS ,  PATTERSON THOMAS A ,  WILLIAMS MARK ,  HERMAN THERON

IPC: A23K1/00 ,  C12N15/52 ,  C12P13/12

CPC classification number: C12P13/12 ,  A23K10/16 ,  A23K20/142 ,  A23K40/10 ,  C12N15/52

Abstract: The present invention relates to microorganisms and processes for the efficient preparation of L-amino acids such as L-methionine. In particular, the present invention relates to microorganisms and processes in which the formation and/or accumulation of homolanthionine in the methionine pathway is reduced and/or prevented.

Abstract translation: 本发明涉及用于有效制备L-氨基酸如L-甲硫氨酸的微生物和方法。 特别地，本发明涉及微生物和其中甲硫氨酸途径中高山ion素的形成和/或积累减少和/或预防的方法。

公开(公告)号：WO2006008097A2

公开(公告)日：2006-01-26

申请号：PCT/EP2005007752

申请日：2005-07-16

Applicant: BASF AG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C12N15/00

CPC classification number: C12N15/63 ,  C07K14/34 ,  C12N15/77 ,  C12P13/04

Abstract: The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the transcription rate and/or expression rate of genes, expression cassettes containing said expression units, genetically modified microorganisms with a modified or induced transcription rate and/or expression rate, and methods for producing biosynthetic products by cultivating the genetically modified microorganisms.

Abstract translation: 本发明涉及使用核酸序列的调节基因，所述新的启动子和表达单元本身的转录和表达，用于改变或引起转录和/或基因的表达率的速率的方法，含有该表达单元的表达盒，具有改变的遗传修饰的微生物或 引起转录速率和/或表达的速率以及通过培养遗传修饰的微生物的制备生物合成产物的方法。

公开(公告)号：WO2005059154A3

公开(公告)日：2005-10-13

申请号：PCT/IB2004004426

申请日：2004-12-17

Applicant: BASF AG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N9/04 ,  C12P13/08 ,  C12N1/21 ,  C12N15/53

CPC classification number: C12N9/0006 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., lactate dehydrogenase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of the expression of lactate dehydrogenase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

Abstract translation: 本发明的特征在于通过使酶编码基因（即乳酸脱氢酶）失调来增加精细化学品例如来自微生物例如棒状杆菌的赖氨酸的生产方法。 在优选的实施方案中，本发明提供通过乳酸脱氢酶活性的表达增加谷氨酸棒杆菌中赖氨酸的产生的方法。 本发明还提供了通过调节向草酰乙酸（OAA）的碳通量来生产赖氨酸的新方法。 在优选的实施方案中，本发明提供了通过使用果糖或蔗糖作为碳源来生产赖氨酸的方法。