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公开(公告)号:US20220111380A1
公开(公告)日:2022-04-14
申请号:US17499454
申请日:2021-10-12
Applicant: Bio-Rad Europe GmbH , Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique , Ronald Lebofsky , Emilie Celine Claudie Macke , Rebecca Dievart , Christophe Quiring , Sophie Pierre
IPC: B01L3/00
Abstract: A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.
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公开(公告)号:US11300496B2
公开(公告)日:2022-04-12
申请号:US17357287
申请日:2021-06-24
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique
IPC: G01N15/14 , C12M1/00 , G01N1/20 , G01N1/28 , G01N1/40 , B01L3/02 , B01L3/00 , G01N35/00 , G06V20/69 , G01N15/00 , G01N15/10
Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
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公开(公告)号:US11199532B2
公开(公告)日:2021-12-14
申请号:US17323176
申请日:2021-05-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique , Kyle Gleason , Christopher Siemer , Priyadarshini Gogoi , Saedeh Sepehri Javdani , Gene Parunak , Aaron Kehrer , Jon Meines
IPC: G01N21/64 , G01N21/25 , G02B21/06 , G02B21/26 , A61B5/00 , G01N21/76 , G01N33/483 , G01N33/487 , G01N33/48
Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.
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公开(公告)号:US20210356383A1
公开(公告)日:2021-11-18
申请号:US17387716
申请日:2021-07-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique
Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
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公开(公告)号:US20210040567A1
公开(公告)日:2021-02-11
申请号:US17077095
申请日:2020-10-22
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique , Priyadarshini Gogoi , Yi Zhou , Saedeh Sepehri , Christopher Siemer
IPC: C12Q1/6886 , C12Q1/6841 , G01N33/574 , B01L3/00 , C12Q1/6834
Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.
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公开(公告)号:US20200271637A1
公开(公告)日:2020-08-27
申请号:US15931928
申请日:2020-05-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique , Kyle Gleason , Christopher Siemer , Priyadarshini Gogoi , Saedeh Sepehri Javdani , Gene Parunak , Aaron Kehrer , Jon Meines
IPC: G01N33/483 , G01N21/64
Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.
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公开(公告)号:US12064760B2
公开(公告)日:2024-08-20
申请号:US18075009
申请日:2022-12-05
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique , Ronald Lebofsky
CPC classification number: B01L3/50255 , B01L7/52 , C12Q1/686 , B01L2200/021 , B01L2200/026 , B01L2200/0652 , B01L2200/0689 , B01L2300/041 , B01L2300/046 , B01L2300/069 , B01L2300/0819 , B01L2300/0893 , B01L2300/168 , B01L2300/18 , B01L2300/1816
Abstract: The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.
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公开(公告)号:US20230407372A1
公开(公告)日:2023-12-21
申请号:US18242658
申请日:2023-09-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique , John Stark , Yadwinder Deol , Vishal Sharma
IPC: C12Q1/6811 , C12Q1/6874 , C12N15/10
CPC classification number: C12Q1/6811 , C12Q1/6874 , C12Q2563/179 , C12Q2565/629 , C12Q2600/178 , C12N15/1065
Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.
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公开(公告)号:US20230314302A1
公开(公告)日:2023-10-05
申请号:US18195084
申请日:2023-05-09
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique
CPC classification number: G01N15/1484 , B01L3/021 , B01L3/502715 , B01L3/502746 , B01L3/502761 , C12M47/04 , G01N1/20 , G01N1/28 , G01N1/40 , G01N1/405 , B01L2200/0668 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0877 , G01N1/4077 , G01N2015/149 , G01N2035/00158
Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
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公开(公告)号:US20230314301A1
公开(公告)日:2023-10-05
申请号:US18195048
申请日:2023-05-09
Applicant: Bio-Rad Laboratories, Inc.
Inventor: Kalyan Handique
CPC classification number: G01N15/1484 , B01L3/021 , B01L3/502715 , B01L3/502746 , B01L3/502761 , C12M47/04 , G01N1/20 , G01N1/28 , G01N1/40 , G01N1/405 , B01L2200/0668 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0877 , G01N1/4077 , G01N2015/149 , G01N2035/00158
Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
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