公开(公告)号：WO1998050582A1

公开(公告)日：1998-11-12

申请号：PCT/US1997012722

申请日：1997-07-21

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  DERVAN, Peter

IPC: C12Q01/68

CPC classification number: C07K7/02 ,  A61K38/00 ,  A61K47/645 ,  C07D207/34 ,  C07D233/90 ,  C07D403/14

Abstract: Methods and compositions are provided for forming complexes between dsDNA and oligomers of heterocycles, aliphatic amino acids, particularly omega-amino acids, and a polar end group. By appropriate choice of target sequences and composition of the oligomers, complexes are obtained with low dissociation constants. The formation of complexes can be used for identification of specific dsDNA sequences, for inhibiting gene transcription, and as a therapeutic for inhibiting proliferation of undesired cells or expression of undesired genes.

Abstract translation: 提供方法和组合物用于在双链DNA和杂环的低聚物，脂族氨基酸，特别是ω-氨基酸和极性端基之间形成复合物。 通过适当选择靶序列和低聚物的组成，可以获得具有低解离常数的复合物。 复合物的形成可用于鉴定特异性dsDNA序列，用于抑制基因转录，以及用作抑制不期望细胞增殖或不期望的基因表达的治疗剂。

公开(公告)号：WO1998050058A1

公开(公告)日：1998-11-12

申请号：PCT/US1997012733

申请日：1997-07-21

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  DERVAN, Peter ,  GOTTESFIELD, Joel, M.

IPC: A61K38/00

CPC classification number: C12N15/63 ,  A61K38/00 ,  A61K47/645 ,  C07D207/34 ,  C07D233/90 ,  C07D403/14 ,  C07K7/02

Abstract: Methods and compositions are provided for forming complexes intracellularly between dsDNA and oligomers of heterocycles, aliphatic amino acids, particularly omega-amino acids, and a polar end group. By appropriate choice of target sequences and composition of the oligomers, complexes are obtained with low dissociation constants. The formation of complexes can be used for modifying the phenotype of cells, either prokaryotic or eukaryotic, for research and therapy.

Abstract translation: 提供了用于在dsDNA和杂环，脂族氨基酸，特别是ω-氨基酸的低聚物和极性端基之间在细胞内形成复合物的方法和组合物。 通过适当选择靶序列和低聚物的组成，可以获得具有低解离常数的复合物。 复合物的形成可用于改变原核或真核细胞的表型，用于研究和治疗。

公开(公告)号：WO1998036822A1

公开(公告)日：1998-08-27

申请号：PCT/US1997019549

申请日：1997-10-23

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  SMITH, Steven, Joel ,  CHUTJIAN, Ara

IPC: B01D59/44

CPC classification number: H01J49/421

Abstract: A mass and velocity analyzer (48) has a cell (60) having four walls (62, 64, 66, 68) applied with time dependent RF potentials. The time dependent RF potentials create an RF field effectively rotating in the cell (60). The rotating RF field disperses an incident ion beam (46) accelerated into the cell (60) according to the mass-to-charge ratio and velocity distribution of ions (52) in the ion beam (46). The ions (52) of the ion beam (46) either collide with an ion detector (54) or deflect away from the ion detector (54), depending on the mass-to-charge ratio, RF amplitude and RF frequency.

Abstract translation: 质量和速度分析器（48）具有具有应用时间相关RF电位的四个壁（62,64,66,68）的单元（60）。 时间相关的RF电位产生在电池（60）中有效旋转的RF场。 旋转RF场根据离子束（46）中离子（52）的质荷比和速度分布，将入射离子束（46）分散到电池（60）中。 离子束（46）的离子（52）与离子检测器（54）碰撞或偏离离子检测器（54），这取决于质量 - 电荷比，RF振幅和RF频率。

公开(公告)号：WO1998021521A1

公开(公告)日：1998-05-22

申请号：PCT/US1997020596

申请日：1997-11-10

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  MARDER, Seth, R. ,  PERRY, Joseph, W.

IPC: F21V9/00

CPC classification number: G11B7/25 ,  B82Y10/00 ,  G11B7/244 ,  G11B7/248

Abstract: Disclosed are highly efficient multi-photon absorbing compounds and methods of their use. The compounds generally include a bridge of pi-conjugated bonds connecting electron donating groups or electron accepting groups. The bridge may be substituted with electron donating groups or electron accepting groups. Solubility, lipophilicity, absorption maxima and other characteristics of the compounds may be tailored by changing the electron donating groups or electron accepting groups or the nature of the pi-conjugated bridge. Numerous photophysical and photochemical methods are enabled by converting these compounds to electronically excited states upon simultaneous absorption of at least two photons of radiation.

Abstract translation: 公开了高效多光子吸收化合物及其使用方法。 化合物通常包括连接供电子基团或电子接受基团的π-共轭键桥。 该桥可以被给电子基团或电子接受基团取代。 化合物的溶解性，亲油性，吸收最大值和其他特征可以通过改变给电子基团或电子接受基团或π-共轭桥的性质来定制。 通过在同时吸收至少两个光子的光子时将这些化合物转化为电子激发态，能够实现许多光物理和光化学方法。

公开(公告)号：WO1998021214A1

公开(公告)日：1998-05-22

申请号：PCT/US1997020390

申请日：1997-11-10

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  GRUBBS, Robert, H. ,  BELDERRAIN, Tomas, R. ,  BROWN, Seth, N. ,  WILHELM, Thomas, E.

IPC: C07F09/02

CPC classification number: B01J31/2404 ,  B01J31/2265 ,  B01J31/2295 ,  B01J31/24 ,  B01J2231/14 ,  B01J2231/54 ,  B01J2231/543 ,  B01J2531/821 ,  B01J2531/825 ,  C07F15/002 ,  C07F15/0046

Abstract: The present invention relates to the synthesis of highly active ruthenium and osmium carbene metathesis catalyst in good yield from readily available starting materials. The catalysts that may be synthesized are of general formula (I) wherein: M is ruthenium or osmium; X and X are independently any anionic ligand; L and L are any neutral electron donor ligand; and, R and R are each hydrogen or one of the following substituent groups: C2-C20 alkenyl, C2-C20 alkynyl, C1-C20 alkyl, aryl, C1-C20 carboxylate, C1-C20 alkoxy, C2-C20 alkenyloxy, C2-C20 alkynyloxy, aryloxy, C2-C20 alkoxycarbonyl, C1-C20 alkylthio, C1-C20 alkylsulfonyl and C1-C20 alkylsufinyl. Optionally, the substituent group may be substituted with one or more groups selected from C1-C5 alkyl, halogen, C1-C5 alkoxy, and aryl. When the substitute aryl group is phenyl, it may be further substituted with one or more groups selected from a halogen, a C1-C5 alkyl, or a C1-C5 alkoxy. Specific synthetic protocols for vinyl alkylidene catalysts wherein R is hydrogen and R is -CHCR R and non-vinyl alkyldiene catalysts are also disclosed. In addition to the ease of synthesis (typically a one-step synthesis), the reactions generally may be run at or above room temperature and the resulting products usually may be used without extensive post synthesis purification.

Abstract translation: 本发明涉及从容易获得的原料合成高活性钌和碳卡因复分解催化剂的良好产率。 可以合成的催化剂具有通式（I），其中：M是钌或锇; X和X 1独立地是任何阴离子配体; L和L 1是任何中性电子给体配体; 和R 1和R 1各自为氢或以下取代基之一：C 2 -C 20烯基，C 2 -C 20炔基，C 1 -C 20烷基，芳基，C 1 -C 20羧酸酯，C 1 -C 20烷氧基，C 2 -C 20链烯氧基 ，C 2 -C 20炔氧基，芳氧基，C 2 -C 20烷氧基羰基，C 1 -C 20烷硫基，C 1 -C 20烷基磺酰基和C 1 -C 20烷基磺酰基。 任选地，取代基可以被一个或多个选自C 1 -C 5烷基，卤素，C 1 -C 5烷氧基和芳基的基团取代。 当取代芳基为苯基时，可以进一步被一个或多个选自卤素，C 1 -C 5烷基或C 1 -C 5烷氧基的基团取代。 还公开了其中R是氢和R 1是-CHR 12 R 13的乙烯基亚烷基催化剂和非乙烯基烷基二烯催化剂的具体合成方案。 除了易于合成（通常是一步合成）之外，反应通常可以在室温或更高的温度下进行，所得产物通常可以在没有广泛的后合成纯化的情况下使用。

公开(公告)号：WO1997046713A1

公开(公告)日：1997-12-11

申请号：PCT/US1997009908

申请日：1997-06-06

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  DAVIDSON, Eric, H.

IPC: C12Q01/68

CPC classification number: C07K16/00

Abstract: The present invention is directed to methods and compositions useful for altering the transcriptional expression of genes in eukaryotic cells. The invention employs novel antibody derivative molecules which function to recognize and bind to specific cis-regulatory DNA sequence elements of a eukaryotic gene. When two antibody derivative molecules are bound to adjacent cis-regulatory DNA sequence elements of a gene, those molecules may interact to form an antibody binding site which is capable of recognizing and binding to a transcription factor protein for the target gene, thereby affecting the functionality of that transcription factor protein and, in turn, the transcriptional activity of the gene. Also provided herein are isolated nucleic acids encoding the novel antibody derivative molecules of the present invention and expression vectors comprising those nucleic acids.

Abstract translation: 本发明涉及可用于改变真核细胞中基因的转录表达的方法和组合物。 本发明使用新型抗体衍生物分子，其功能识别并结合真核基因的特异性顺式调节DNA序列元件。 当两个抗体衍生分子结合到基因的相邻顺式调节DNA序列元件时，那些分子可以相互作用以形成抗体结合位点，其能够识别并结合靶基因的转录因子蛋白，从而影响功能 的转录因子蛋白，又是基因的转录活性。 本文还提供了编码本发明的新型抗体衍生物分子的分离的核酸和包含那些核酸的表达载体。

公开(公告)号：WO1997035299A1

公开(公告)日：1997-09-25

申请号：PCT/US1997004636

申请日：1997-03-20

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  GOODMAN, Rodney, M. ,  SPANGLER, Randall, R.

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: G10H01/00

CPC classification number: G10H1/0025 ,  G10H1/0066 ,  G10H1/38 ,  G10H2210/105 ,  G10H2210/111 ,  G10H2210/136 ,  G10H2210/145 ,  G10H2240/295 ,  G10H2250/311 ,  Y10S84/09

Abstract: A music composition system (1), comprising receiving a first harmony including a first melody, analyzing the first harmony to derive in real-time a rule relating the first melody to the first harmony, receiving a second melody, and applying the rule in real-time to the second melody to produce a second harmony relating to the second melody.

Abstract translation: 一种音乐作曲系统（1），包括接收包括第一旋律的第一和声，分析第一和声以实时获得与第一旋律相关的规则与第一和声，接收第二旋律，并将该规则应用于实际 - 第二个旋律，以产生与第二个旋律相关的第二个和声。

公开(公告)号：WO1997033177A1

公开(公告)日：1997-09-12

申请号：PCT/US1997003654

申请日：1997-03-03

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  ARNOLD, Frances, H. ,  GUAN, Zhibin ,  CHEN, Chao-Tsen ,  CHEN, Guohua

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: G01N33/66

CPC classification number: G01N33/68 ,  C12Q1/006 ,  G01N33/66 ,  G01N2600/00 ,  G01N2650/00

Abstract: Sensors (20, 50, 70) for use in detecting the presence of sugars and other analytes (target molecules). The sensor is composed of a metal complex that binds to the target molecule and releases a proton or includes an exchangable ligand which is exchanged for the target molecule during the binding interaction between the metal complex and the target molecule. The result of the binding interaction is the release of a proton, hydroxide ion or ligand species generated during the ligand exchange. Measurement of the release of proton, hydroxide ion or other ligand species from the sensor (20, 50, 70) provides an indirect indication of target molecule concentration. The metal complexes may be attached to support structures (10, 12) to provide both anchoring and positioning of the metal ions to increase selectivity of sugar/metal complex interactions. Detection systems in which pH is used as an indication of proton or hydroxide release are disclosed, as are detection systems in which Cl release is used. Methods for monitoring the concentrations of sugars and related molecules using the metal based sensors (20, 50, 70) are also disclosed.

Abstract translation: 用于检测糖和其他分析物（靶分子）的存在的传感器（20,50,70）。 该传感器由结合目标分子并释放质子的金属络合物组成，或包括在金属络合物和靶分子之间的结合相互作用期间与靶分子交换的可交换配体。 结合相互作用的结果是在配体交换期间产生的质子，氢氧根离子或配体物质的释放。 从传感器（20,50,70）释放质子，氢氧根离子或其他配体物质的测量提供了目标分子浓度的间接指示。 金属络合物可以连接到支撑结构（10,12），以提供金属离子的锚定和定位，以增加糖/金属络合物相互作用的选择性。 公开了使用pH作为质子或氢氧化物释放指示的检测系统，其中使用Cl - 释放的检测系统也是如此。 还公开了使用金属基传感器（20,50,70）监测糖和相关分子的浓度的方法。

公开(公告)号：WO1997021256A1

公开(公告)日：1997-06-12

申请号：PCT/US1996019731

申请日：1996-12-09

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  SURAMPUDI, Subbarao ,  FRANK, Harvey, A. ,  NARAYANAN, Sekharipuram, R. ,  CHUN, William ,  JEFFRIES-NAKAMURA, Barbara ,  KINDLER, Andrew ,  HALPERT, Gerald

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: H01M04/92

CPC classification number: H01M8/2455 ,  H01M4/8605 ,  H01M4/8807 ,  H01M4/8821 ,  H01M4/8839 ,  H01M4/8871 ,  H01M4/921 ,  H01M4/926 ,  H01M8/0206 ,  H01M8/0213 ,  H01M8/0223 ,  H01M8/0228 ,  H01M8/0234 ,  H01M8/0239 ,  H01M8/0241 ,  H01M8/0245 ,  H01M8/0247 ,  H01M8/0269 ,  H01M8/04007 ,  H01M8/04186 ,  H01M8/04194 ,  H01M8/04373 ,  H01M8/04447 ,  H01M8/04582 ,  H01M8/04873 ,  H01M8/1004 ,  H01M8/1009 ,  H01M8/1011 ,  H01M8/1023 ,  H01M8/1039 ,  H01M8/22 ,  H01M2300/0082 ,  Y02E60/523 ,  Y10T29/49108

Abstract: Improvements to non-acid fuel methanol fuel cells (10) include new formulations for the materials used therein. The anode (14) includes platinum and rithenium uniformly mixed together. Other materials may be substituted for these metals. The cathode (16) is made of platinum particles, and is bonded to a side of the solid polymer electrolyte membrane (18). The backing material for the fuel electrode is treated to improve its characteristics. An extremely porous electrode made by sputtering is also disclosed.

Abstract translation: 对非酸性燃料甲醇燃料电池（10）的改进包括用于其中的材料的新配方。 阳极（14）包括铂和铼均匀混合在一起。 其他材料可以代替这些金属。 阴极（16）由铂颗粒制成，并且与固体聚合物电解质膜（18）的一侧结合。 处理用于燃料电极的背衬材料以改善其特性。 还公开了通过溅射制成的极其多孔的电极。

公开(公告)号：WO1997017800A1

公开(公告)日：1997-05-15

申请号：PCT/US1996018287

申请日：1996-11-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  YADID-PECHT, Orly ,  FOSSUM, Eric, C.

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: H04N03/143

CPC classification number: H04N5/3535 ,  H04N5/235 ,  H04N5/35572 ,  H04N5/3742 ,  H04N5/3745 ,  H04N5/378

Abstract: Designs and operational methods to increase the dynamic range of image sensors (200) and APS devices (300) in particular by achieving more than one integration times for each pixel (302) thereof. An APS system (200) with more than one column-parallel signal chains (204, 206) for readout are described for maintaining a high frame rate in readout. Each active pixel (302) is sampled for multiple times during a single frame readout, thus resulting in multiple integration times. The operation methods can also be used to obtain multiple integration times for each pixel (302) with an APS design having a single column-parallel signal chain (104) for readout. Furthermore, analog-to-digital conversion of high speed and high resolution can be implemented.

Abstract translation: 特别是通过为每个像素（302）实现多于一个的积分时间来增加图像传感器（200）和APS装置（300）的动态范围的设计和操作方法。 描述了具有用于读出的多个列并行信号链（204,206）的APS系统（200），用于在读出中保持高帧速率。 在单帧读出期间，每个有源像素（302）被采样多次，从而导致多个积分时间。 操作方法还可以用于通过具有用于读出的单个列并行信号链（104）的APS设计为每个像素（302）获得多个积分时间。 此外，可以实现高速和高分辨率的模数转换。