Abstract:
A new method and apparatus for the injection molding of semi-solid materials (SSM). In this process (called Rheomolding), a superheated liquid metal is cooled into the semi-solid state in the barrel of a special vertical injection-molding machine, with the growing dendrites of the solid phase being broken into small and nearly spherical particles by the shearing force generated by the screw and barrel. Compared with the superheated liquid metal, SSM has lower temperature, lower shrinkage and a more stable flow pattern. Therefore, the rheomolding process can produce net-shape metal or metal-matrix-composite parts continuously at lower cost.
Abstract:
Microsatellite nucleotide repeat sequences present in DNA isolated from a biological sample are used to determine the origin of a biological sample by a process including the steps of isolating nucleic acid from a biological sample, determining the interspersion pattern of repeats of a nucleotide sequence selected from the group consisting of di-, tri-, and tetra-microsatellite nucleotide repeat sequences in the DNA, and comparing the determined interspersion pattern with known interspersion patterns of the nucleotide sequence in selected mammalian species. Species-specific, individually-invariant interspersion patterns of di-, tri-, or tetra-microsatellite nucleotide repeats in vertebrate DNA samples are identified by a process including the step of annealing separated strands with an oligonucleotide primer selected from the group consisting of di-, tri- and tetra-microsatellite nucleotide repeat sequences at a concentration ratio of primer to DNA template of less than about 2500:1, the annealing being conducted at a temperature greater than about 30 DEG C and in the presence of magnesium ions at a concentration less than about 3.0 mM, to produce an extension product of the primer which is complementary to the strand of the DNA sample.
Abstract:
A novel sequence in the genome of Marek's disease virus (MDV) containing an immediate early gene associated with lytic infection and/or tumor cell development of MDV-infected cells, is described. The location of this sequence in the MDV genome is shown in the figure. The deduced amino acid sequence of the 94 amino acid protein, encoded by this novel sequence, is also disclosed. Disclosed are the use of the novel sequence and the 94 amino acid protein in strategies to control Marek's disease such as by vaccination.
Abstract:
A polymerase chain reaction-coupled ligase chain reaction is described for the identification of microorganisms based upon single-base-pair differences in the genetic information of each organism.
Abstract:
A continuous wave tunable femtosecond optical parametric oscillator (10) includes an oscillator cavity including a pumping cavity (20). A nonlinear KTiOAsO crystal (KTA) (24) is located in the pumping cavity (20) and is pumped by a Ti:sapphire laser (30) to produce output pulses having widths as short as 85 femtoseconds at wavelengths in the range 1.0 mu m to 5.0 mu m.
Abstract:
A method of pore-size selective chemical modification of porous materials is disclosed. The resulting novel porous materials are particularly useful as separation media in chromatography, for selective isolation, adsorption and catalysis.
Abstract:
A process for carrying out in a consecutive fashion different modes of chromatographic separation in a liquid chromatography column using a single separation medium is disclosed. Separation media for use in such multimodal separations are also disclosed.
Abstract:
The present invention relates to a method of conferring resistance against fire blight to pomaceous fruit scion or rootstock cultivars by transforming such cultivars with a gene which encodes for lytic proteins. Such transformation can be effected by bacterial infection or propulsion of particles into cell interiors. Once transformation has taken place, the cultivar is regenerated to a transgenic pomaceous fruit tree. This technique is particularly useful in treating apple and pear cultivars.
Abstract:
The present invention relates to a new variety of retroviruses distinct from HIV-1 and HIV-2, designated HIV-LP. The isolation, characterization and cloning of a prototype HIV-LP is described. The invention also relates to nucleotide sequences derived from the HIV-LP family, viral proteins and antigens and antibodies specific for HIV-LP which can be used for diagnostic and/or therapeutic purposes.
Abstract:
A precision microactuator (30) fabricated from a single crystal of silicon for mechanically moving microstructures (10) having a beam (12) with a longitudinal axis and a first fixed end (14) and a second remote end, there being at least one pair of opposed capacitor plates (40, 42) in the beam dividing the beam into relatively movable axially aligned, longitudinal segments (12', 12', and 12'''), and electrical connections (70) for applying an electric potential across the plates to cause the relatively movable segments to move with respect to each other to cause the remote end of the beam to move along the longitudinal axis with respect to the fixed end to vary the length of the beam.