公开(公告)号：WO1998023633A1

公开(公告)日：1998-06-04

申请号：PCT/US1997022335

申请日：1997-11-25

Applicant: CORNELL RESEARCH FOUNDATION, INC.

Inventor： CORNELL RESEARCH FOUNDATION, INC. ,  MARK, Willie, H. ,  HOLZSCHU, Donald, L.

IPC: C07H21/04

CPC classification number: C12N15/1051 ,  A01K2217/05 ,  A01K2267/0393 ,  C12N15/8509 ,  C12Q1/6897

Abstract: The present invention provides methods for identifying human developmental genes. The invention also provides methods of obtaining mammals having a promoterless reporter gene expressed from a gene which is homologous to a human developmental gene. Fragments of several embryonic stem cell transcripts which show developmental or tissue specific regulation are also disclosed.

Abstract translation: 本发明提供鉴定人发育基因的方法。 本发明还提供了获得具有与人发育基因同源的基因表达的无启动子报告基因的哺乳动物的方法。 还公开了显示发育或组织特异性调节的几种胚胎干细胞转录物的片段。

公开(公告)号：WO1998021585A1

公开(公告)日：1998-05-22

申请号：PCT/US1997021351

申请日：1997-11-14

Applicant: CORNELL RESEARCH FOUNDATION, INC.

Inventor： CORNELL RESEARCH FOUNDATION, INC. ,  TAYLOR, Stephen, J. ,  SHALLOWAY, David

IPC: G01N33/53

CPC classification number: G01N33/57484 ,  G01N33/574 ,  G01N2333/47 ,  G01N2333/4703 ,  G01N2333/82

Abstract: The present invention relates to a method for detecting activated ras protein. The method includes immobilizing a protein on a solid support, incubating the immobilized protein with lysates from cultured cells, where the lysates include activated ras protein, and determining the amount of activated ras protein bound to the immobilized protein. The present invention also relates to a method of detecting ras oncogenic related malignancy in a human subject.

Abstract translation: 本发明涉及一种检测活化的ras蛋白的方法。 该方法包括将蛋白质固定在固体支持物上，将固定的蛋白质与来自培养细胞的裂解物孵育，其中裂解物包括活化的ras蛋白质，以及测定与固定化蛋白质结合的活化的ras蛋白的量。 本发明还涉及在人类受试者中检测ras致癌相关恶性肿瘤的方法。

公开(公告)号：WO1998020164A1

公开(公告)日：1998-05-14

申请号：PCT/US1997020156

申请日：1997-11-04

Applicant: CORNELL RESEARCH FOUNDATION, INC.

Inventor： CORNELL RESEARCH FOUNDATION, INC. ,  WEEDEN, Norman, F. ,  LOOMIS, Dale ,  CELESTE, Joseph, A.

IPC: C12Q01/68

CPC classification number: B01L3/5085 ,  B03C1/24 ,  C12M47/06 ,  G01N1/286 ,  G01N35/0098

Abstract: The device and methods herein disclosed were developed to allow a large number of small samples of organic tissue to be processed. The goal was to allow for the capture of these intracellular contents for study, use, and/or amplification. Though the device and specific protocols can be used to retrieve a variety of intracellular molecules one of the preferred embodiments particularly lends itself to the extraction of DNA. The electromagnetic device uses a set of terraced coils controlled in such a manner as to continually alternate polarity and thereby emulate the random motion of a manual mortar and pestle. This simulation of random motion is created through the arrangement of coils found in this invention. Methods were also developed to aid in the retrieval of DNA or other intracellular components using this device such that these components can be isolated and then used for study, amplification, or a variety of different purposes. Essentially the enclosed invention discloses a device and methodology which will allow for the rapid processing of many small tissue samples. This will assist in making such procedures as genotyping, genetic analysis, and gene mapping faster and more reliable on a large scale.

Abstract translation: 本文公开的装置和方法被开发以允许处理大量有机组织的小样本。 目的是允许这些细胞内内容物的捕获用于研究，使用和/或扩增。 尽管该装置和具体方案可用于检索各种细胞内分子，但优选实施方案中的一个特别适用于提取DNA。 电磁装置使用一组梯形线圈，其以这样的方式进行控制，以连续地交替极性，从而模拟手动砂浆和杵的随机运动。 随机运动的这种模拟通过本发明中发现的线圈的布置产生。 还开发了方法以帮助使用该装置检索DNA或其他细胞内组分，使得这些组分可以被分离，然后用于研究，扩增或各种不同的目的。 本质上，所附的发明公开了一种将允许许多小组织样品的快速处理的装置和方法。 这将有助于大规模地进行基因分型，遗传分析和基因定位等过程更快更可靠的程序。

公开(公告)号：WO1998012341A1

公开(公告)日：1998-03-26

申请号：PCT/US1997016282

申请日：1997-09-15

Applicant: CORNELL RESEARCH FOUNDATION, INC.

Inventor： CORNELL RESEARCH FOUNDATION, INC. ,  CESARMAN, Ethel ,  ARVANITAKIS, Leandros ,  KNOWLES, Daniel, M. ,  MESRI, Enrique

IPC: C12P07/62

CPC classification number: C12N7/00 ,  C12N2710/16451

Abstract: The invention provides a cell line comprising Kaposi's sarcoma-associated herpesvirus (KSHV). Preferably, the cell line is a body cavity-based lymphoma cell line, and more preferably, the cell line does not harbor Epstein-Barr virus. Two cell lines designated BC-2 and BC-3 are thus provided. These cell lines can be used in a method of propagating KSHV. The method comprises culturing the cell line, wherein the KSHV within the cell line thereby propagates. The BC-3 cell line, in particular, can be used in a method of propagating KSHV in the absence of Epstein-Barr virus. The method comprises culturing the cell line designated BC-3, wherein the KSHV within the cell line thereby propagates. Further provided is a purified viral suspension of KSHV, as well as a composition comprising purified KSHV and a suitable carrier.

Abstract translation: 本发明提供了包含卡波西肉瘤相关疱疹病毒（KSHV）的细胞系。 优选地，细胞系是基于体腔的淋巴瘤细胞系，更优选地，细胞系不包含爱泼斯坦 - 巴尔病毒。 因此提供了两个指定为BC-2和BC-3的细胞系。 这些细胞系可以用于传播KSHV的方法。 该方法包括培养细胞系，其中细胞系内的KSHV由此传播。 特别是BC-3细胞系可以用于在没有爱泼斯坦 - 巴尔病毒的情况下传播KSHV的方法。 该方法包括培养称为BC-3的细胞系，其中细胞系内的KSHV由此传播。 还提供了KSHV的纯化病毒悬浮液，以及包含纯化的KSHV和合适的载体的组合物。

公开(公告)号：WO1998011214A1

公开(公告)日：1998-03-19

申请号：PCT/IB1997001217

申请日：1997-09-11

Applicant: THOMAS JEFFERSON UNIVERSITY ,  CORNELL RESEARCH FOUNDATION, INC.

Inventor： THOMAS JEFFERSON UNIVERSITY ,  CORNELL RESEARCH FOUNDATION, INC. ,  HOLLOMAN, William, K. ,  RICE, Michael, C. ,  SMITH, Sheryl, T. ,  SHU, Zhigang ,  KMIEC, Eric, B.

IPC: C12N15/12

CPC classification number: C12N9/00 ,  A01K2217/05

Abstract: The invention concerns mammalian recombinase genes (REC2) and their promoters. Over expression of REC2 in a cell is found to facilitate homologous recombination, particularly homologous recombination using a DNA/RNA chimeric oligonucleotide and to sensitize a cell to the apoptotic effects of irradiation. The REC2 promoter, in combination with a strong enhancer, e.g., a SV40 enhancer, was found to be a strong promoter following irridiation of the cells. A radiation induceable promoter can be used to sensitize a cell to radiation treatment by operably linking the radiation induceable promoter to a gene whose expression converts a prodrug to a drug such as a herpes thymidine kinase gene.

Abstract translation: 本发明涉及哺乳动物重组酶基因（REC2）及其启动子。 发现细胞中REC2的过表达促进同源重组，特别是使用DNA / RNA嵌合寡核苷酸的同源重组，并使细胞对辐照的凋亡作用敏感。 发现REC2启动子与强增强子（例如SV40增强子）组合，在细胞灌注后是强启动子。 辐射诱导启动子可用于通过将辐射诱导启动子可操作地连接到表达将前药转化为药物如疱疹胸苷激酶基因的基因来使细胞对辐射治疗敏感。

公开(公告)号：WO1998008613A1

公开(公告)日：1998-03-05

申请号：PCT/US1997014997

申请日：1997-08-27

Applicant: CORNELL RESEARCH FOUNDATION, INC. ,  VALASKOVIC, Gary, A. ,  McLAFFERTY, Fred, W.

Inventor： CORNELL RESEARCH FOUNDATION, INC.

IPC: B05B05/00

CPC classification number: H01J49/167 ,  B05B5/0255 ,  G01N27/44717

Abstract: An ultra-low flow rate electrospray ionization (ESI) source provides flow rates in the range of 1.0 nL/min or less. The source is comprised of a needle (12) which is fabricated by laser-heated pulling of fused-silica tubing, followed by chemical etching and surface metallization. The pulling results in formation of a slowly tapering capillary (40) within the needle (12) which tapers to a tip (22) having a very small inner diameter. The etching process sharpens the outer wall (42) of the needle (12) to a very sharp tip, and the combination of these parameters results in the ultra-low flow rate capability. After a metal electrical contact (44) is formed on the exterior wall of the needle (12), an electrically insulating overcoating (46) is preferably deposited thereon which locks the contact (44) in place, thereby greatly increasing needle life, and also restricting the electrical contact point to the very tip of the needle (12). Although the use of the ultra-low flow rate ESI sources increases sensitivity to sampling errors, a mechanism is also provided to minimize one primary source of such errors, evaporation induced hydrodynamic flow, in capillary electrophoresis (CE). An injection system (60) is provided which enables a retractable droplet of buffer solvent to be positioned in contact with the tip end of the ESI needle during sample loading. This prevents evaporation from the tip end, thereby eliminating hydrodynamic flow into the distal end (63) of the capillary column (62) used in the CE process.

Abstract translation: 超低流速电喷雾电离（ESI）源提供1.0nL / min以下的流速。 该源由针（12）组成，其通过激光加热熔化石英管制造，随后进行化学蚀刻和表面金属化。 牵引导致在针（12）内形成缓慢变细的毛细管（40），其逐渐变细到具有非常小的内径的尖端（22）。 蚀刻工艺将针（12）的外壁（42）锐化到非常尖的尖端，并且这些参数的组合导致超低流速能力。 在针（12）的外壁上形成金属电接触（44）之后，优选在其上沉积电绝缘的外涂层（46），其将触点（44）锁定就位，从而大大增加针的寿命，并且还 将电接触点限制到针（12）的尖端。 尽管使用超低流量ESI源增加了对采样误差的敏感性，但也提供了一种机制来最小化毛细管电泳（CE）中这种错误，蒸发诱导的流体动力学流动的一个主要来源。 提供了一种注射系统（60），其在样品加载期间使缓冲溶剂的可收缩液滴定位成与ESI针的末端接触。 这防止了从尖端的蒸发，从而消除了进入CE过程中使用的毛细管柱（62）的远端（63）的流体动力学流动。

公开(公告)号：WO1998003673A1

公开(公告)日：1998-01-29

申请号：PCT/US1997012195

申请日：1997-07-15

Applicant: CORNELL RESEARCH FOUNDATION, INC. ,  PURDUE RESEARCH FOUNDATION

Inventor： CORNELL RESEARCH FOUNDATION, INC. ,  PURDUE RESEARCH FOUNDATION ,  BARANY, Francis ,  LUO, Jianying ,  KHANNA, Marilyn ,  BERGSTROM, Donald, E.

IPC: C12P19/34

CPC classification number: C12Q1/6827 ,  C07K14/82 ,  C12N9/93 ,  C12Q1/6837 ,  C12Q1/6862 ,  C12Q2531/113 ,  C12Q2561/125 ,  C12Q2565/501

Abstract: Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.

Abstract translation: 利用连接酶检测反应在常规模板过量存在下用热稳定连接酶区分少数模板。 该过程可以用突变连接酶，热稳定连接酶或修饰的寡核苷酸探针进行。 该过程对癌症相关突变的检测特别有用。 它具有提供少量模板数量或比例的定量测量的优点。

公开(公告)号：WO1998001738A2

公开(公告)日：1998-01-15

申请号：PCT/US1997010557

申请日：1997-06-19

Applicant: CORNELL RESEARCH FOUNDATION, INC. ,  SMITH, Kendall, A.

Inventor： CORNELL RESEARCH FOUNDATION, INC.

IPC: G01N00/00

CPC classification number: C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/156

Abstract: A method of diagnosing a condition or disease associated with MICROBIAL infections, congenital or acquired immunodeficiencies, inflammatory, auto-immune, allergic, or dermatologic diseases, sarcoidosis, immunoscenesence, sepsis, necrosis, malignancies, or vaccine administration, comprises obtaining a sample comprising T or B cells from a subject suspected of being afflicted with the condition selected from the group consisting of conditions associated with infections by congenital or acquired immunodeficiencies, inflammatory, auto-immune, allergic, or dermatologic diseases, sarcoidosis, immunoscenesence, sepsis, necrosis, malignancies, or vaccine administration.

Abstract translation: 诊断与微生物感染，先天性或后天免疫缺陷，炎症，自身免疫，过敏性或皮肤病，结节病，免疫血症，败血症，坏死，恶性肿瘤或疫苗施用相关的病症或疾病的方法包括获得包含T 或来自被怀疑患有由先天性或后天免疫缺陷感染，炎性，自身免疫，过敏性或皮肤病，结节病，免疫荧光，败血症，坏死，恶性肿瘤的病症组成的组的患者的B细胞 ，或疫苗管理。

公开(公告)号：WO1997015303A1

公开(公告)日：1997-05-01

申请号：PCT/US1996016309

申请日：1996-10-21

Applicant: CORNELL RESEARCH FOUNDATION, INC.

Inventor： CORNELL RESEARCH FOUNDATION, INC. ,  REGUNATHAN, Soundararajan ,  REIS, Donald, J.

IPC: A61K31/415

CPC classification number: A61K31/498 ,  A61K31/00 ,  A61K31/155 ,  A61K31/415 ,  A61K31/4174 ,  A61K31/4178

Abstract: Disorders mediated by vascular smooth muscle proliferation are treated by administering a vascular smooth muscle antiproliferative effective amount of an I2 imidazoline receptor agonist. The disorders include atherosclerosis, risk of blockage of artery after coronary angioplasty or blood vessel injury from non-angioplasty cause, and proliferative diabetic retinopathy. I2 imidazoline receptor agonists include idazoxan, UK 14,304, naphazoline, cirazoline and agmatine.

Abstract translation: 通过施用血管平滑肌抗增殖有效量的1,2咪唑啉受体激动剂治疗由血管平滑肌增生介导的疾病。 这些疾病包括动脉粥样硬化，冠状动脉成形术后动脉阻塞的风险或非血管成形术引起的血管损伤，以及增殖性糖尿病性视网膜病变。 I2咪唑啉受体激动剂包括idazoxan，UK14,304，萘甲唑啉，环唑啉和丁胺。

公开(公告)号：WO1997011355A1

公开(公告)日：1997-03-27

申请号：PCT/US1996014519

申请日：1996-09-18

Applicant: CORNELL RESEARCH FOUNDATION, INC. ,  WEBB, Watt, W. ,  XU, Chris

Inventor： CORNELL RESEARCH FOUNDATION, INC.

IPC: G01N21/64

CPC classification number: G02B21/002 ,  G01N21/6458 ,  G02B21/0076 ,  G02B21/0084

Abstract: A laser scanning micrsocope (10) produces molecular excitation in a target material (14) by simultaneous absorption of three or more photons to thereby provide intrinsic three-dimensional resolution. Fluorophores having single photon absorption in the short (ultraviolet or visible) wavelength range are excited by a beam (16) of strongly focused subpicosecond pulses of laser light of relatively long (red or infrared) wavelength range. The fluorophores absorb at about one third, one fourth or even smaller fraction of the laser wavelength to produce fluoroscent images of living cells and other microscopic objects. The fluoroscent emission from the fluorophores increases cubicly, quarticly or even higher power law with the excitation intensity so that by focusing the laser light, fluorescence as well as photobleaching are confined to the vicinity of the focal plane. This feature provides depth of field resolution comparable to that produced by confocal laser scanning microscopes, and in addition reduces photobleaching and phototoxicity.

Abstract translation: 激光扫描微透镜（10）通过同时吸收三个或更多个光子在目标材料（14）中产生分子激发，从而提供固有的三维分辨率。 在短（紫外或可见）波长范围内具有单光子吸收的荧光团由具有相对较长（红色或红外）波长范围的激光的强烈聚焦的亚秒脉冲的光束（16）激发。 荧光团吸收激光波长的约三分之一，四分之一甚至更小的部分，以产生活细胞和其他微观物体的荧光图像。 来自荧光团的荧光发射随着激发强度增加立方，四方或甚至更高的幂定律，使得通过聚焦激光，荧光和光漂白被限制在焦平面附近。 该特征提供了与共焦激光扫描显微镜产生的景深相当的景深分辨率，并且还减少了光漂白和光毒性。