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    ULTRA-SENSITIVE DETECTION OF ENZYMES BY CAPTURE-AND-RELEASE FOLLOWED BY QUANTIFICATION
    21.
    发明申请
    ULTRA-SENSITIVE DETECTION OF ENZYMES BY CAPTURE-AND-RELEASE FOLLOWED BY QUANTIFICATION 审中-公开
    通过定量检测和释放对酶的超敏感检测

    公开(公告)号：US20100075355A1

    公开(公告)日：2010-03-25

    申请号：US12236490

    申请日：2008-09-23

    Applicant: David C. Duffy ,  Evan Ferrell ,  Jeffrey D. Randall ,  David M. Rissin ,  David R. Walt

    Inventor： David C. Duffy ,  Evan Ferrell ,  Jeffrey D. Randall ,  David M. Rissin ,  David R. Walt

    IPC: C12Q1/34 ,  C12Q1/28

    CPC classification number: G01N33/543 ,  G01N33/54306 ,  G01N33/54393

    Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles to form a plurality of complexes, releasing at least a portion of some of the plurality of complexes, determining at least a portion of the plurality of complexes released, and determining a measure of the concentration of the analyte molecules or particles in a fluid sample.

    Abstract translation: 本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。 本发明的方法可以包括固定多个分析物分子或颗粒以形成多个复合物，释放所述多个复合物中的至少一部分，确定所述多个复合物的至少一部分被释放，并且确定 测量流体样品中分析物分子或颗粒的浓度。

    Controlled alteration of pores using fluid flow and fabrication of articles thereby
    24.
    发明申请
    Controlled alteration of pores using fluid flow and fabrication of articles thereby 有权
    使用流体流动控制孔隙的改变，从而制造制品

    公开(公告)号：US20090215143A1

    公开(公告)日：2009-08-27

    申请号：US11658515

    申请日：2005-07-26

    Applicant: David J. Monk ,  David R. Walt

    Inventor： David J. Monk ,  David R. Walt

    IPC: C12N13/00 ,  B05D1/36 ,  B05D7/22 ,  B05D5/12 ,  G01N27/00 ,  B32B1/08 ,  G02B27/10

    CPC classification number: G01N33/5438 ,  B01J2219/00439 ,  B01J2219/0045 ,  B01J2219/00524 ,  B01J2219/00653 ,  Y10T428/13

    Abstract: The invention relates to microscopic structures and methods of making and using the structures. A method of forming a microscopic structure of a material includes obtaining a solution (310) containing the material, establishing a flowing stream of the solution (310) in a capillary (104), wherein the capillary (104) has an inner dimension that is smaller than about 300 micrometers, and maintaining the stream until a layer is built up along an inner wall of the capillary (104) from material deposited from the flowing stream, thereby forming a microscopic structure.

    Abstract translation: 本发明涉及微观结构及其制造和使用方法。 形成材料的微观结构的方法包括获得含有该材料的溶液（310），在毛细管（104）中形成溶液（310）的流动流，其中毛细管（104）的内部尺寸为 小于约300微米，并且保持流，直到沿着毛细管（104）的内壁从流动物料沉积的材料构建层，从而形成微观结构。

    Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
    27.
    发明授权
    Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample 失效
    用于选择性地检测混合流体样品中的寡核苷酸种类的光纤生物传感器

    公开(公告)号：US06406845B1

    公开(公告)日：2002-06-18

    申请号：US08851203

    申请日：1997-05-05

    Applicant: David R. Walt ,  Brian G. Healey

    Inventor： David R. Walt ,  Brian G. Healey

    IPC: C12Q168

    CPC classification number: G01N21/7703 ,  G01N2201/0833 ,  Y10S977/924

    Abstract: The present invention provides biosensors, apparatus and methods for selectively detecting at least one complementary oligonucleotide target specie in a fluid sample containing a mixture of different oligonucleotide fragments. One preferred embodiment of the biosensor is as a unitary fiber optic array having an in-situ hybridization zone comprising not less than one specie of single stranded oligonucleotide disposed as individual deposits in aligned organization upon multiple strand end faces at differing spatial positions on the distal array end surface. In this manner, a collective of deployed, single specie, multiple fixed probes are presented for selective in-situ hybridization on-demand with at least one mobile complementary target specie ultimately bearing a joined identifying label. The biosensor provides for optical detection of in-situ hybridization on the distal end surface via the presence of the concomitantly disposed joined identifying label at the differing spatial positions.

    Abstract translation: 本发明提供用于选择性检测含有不同寡核苷酸片段的混合物的流体样品中的至少一种互补寡核苷酸靶物种的生物传感器，装置和方法。 生物传感器的一个优选实施方案是具有原位杂交区的单一光纤阵列，其包含不少于一种单链寡核苷酸，所述单链寡核苷酸作为单个沉积物以对齐的组织形式排列在远端阵列上的不同空间位置的多股端面上 端面。 以这种方式，提供了一组部署的单个物种，多个固定探针，用于选择性原位杂交，其中至少一个移动互补目标物种最终具有连接的鉴定标签。 生物传感器通过在不同的空间位置存在伴随设置的连接的识别标签，提供在远端表面上的原位杂交的光学检测。

    Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy
    28.
    发明授权
    Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy 失效
    用于亚波长光能发生和近场光学显微镜的超分辨成像光纤

    公开(公告)号：US5633972A

    公开(公告)日：1997-05-27

    申请号：US564488

    申请日：1995-11-29

    Applicant: David R. Walt ,  Paul Pantano

    Inventor： David R. Walt ,  Paul Pantano

    IPC: G01Q60/22 ,  G02B6/06 ,  G02B6/24

    CPC classification number: G01Q60/22 ,  B82Y20/00 ,  B82Y35/00 ,  G02B6/06 ,  G02B6/241

    Abstract: The present invention provides a superresolution imaging fiber for generating a plurality of subwavelength light energy beams concurrently and for near-field viewing. The imaging fiber comprises a unitary fiber optic array of fixed configuration and dimensions comprising typically from 1,000 to 100,000 optical fiber strands which terminate at one array end as tapered strand end faces limited in size to a range from about 2-1,000 nanometers in diameter. Overlyng these tapered strand end faces is a thin opaque metal coating having a size-limited end aperture ranging from about 2 to less than about 1,000 nanometers in diameter. These size-limited end aperatures provide for the generation of a plurality of discrete subwavelength light beams concurrently.

    Abstract translation: 本发明提供了一种用于同时产生多个亚波长光能束并用于近场观看的超分辨率成像光纤。 成像光纤包括具有固定构造和尺寸的单一光纤阵列，其包括通常为1,000至100,000个光纤线束，其在一个阵列端处终止，其中锥形线束端面的尺寸限制在约2-1,000纳米直径的范围内。 超过这些锥形股线端面是薄的不透明金属涂层，其具有尺寸限制的端部孔径在约2至小于约1000纳米的直径。 这些尺寸限制的端部温度同时提供了多个离散的亚波长光束的产生。

    Absorbance modulated fluorescence detection methods and sensors
    30.
    发明授权
    Absorbance modulated fluorescence detection methods and sensors 失效
    吸光度调制荧光检测方法和传感器

    公开(公告)号：US5143853A

    公开(公告)日：1992-09-01

    申请号：US305176

    申请日：1989-02-02

    Applicant: David R. Walt

    Inventor： David R. Walt

    IPC: G01N21/64 ,  G01N21/77 ,  G01N33/58

    CPC classification number: G01N33/582 ,  G01N21/6428 ,  G01N2021/6434 ,  G01N2021/7793 ,  G01N21/7703 ,  G01N2201/06193 ,  Y10S435/808 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/13

    Abstract: A variety of methods and apparatus for the detection of an analyte of interest in a fluid sample is provided which relies upon the interaction of a fluorophore and a chromophoric light absorbing compound for qualitative and quantitative results. The methods preferably employ fiber optic sensors in combination with fluorophores and/or proto-absorber substances in mobile and immobilized modes of use. The methods and apparatus rely upon the ability of the light absorbing compositions to absorb energy which is transferred either radiatively or non-radiatively by the fluorophore when in an excited state.

    Abstract translation: 提供了用于检测流体样品中感兴趣分析物的各种方法和装置，其依赖于荧光团和发色光吸收化合物的相互作用用于定性和定量结果。 该方法优选采用光纤传感器与移动和固定使用模式中的荧光团和/或原吸收物质组合。 所述方法和装置依赖于吸光组合物在处于激发状态时吸收由荧光团辐射或非辐射转移的能量的能力。

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