公开(公告)号：US5571767A

公开(公告)日：1996-11-05

申请号：US517737

申请日：1995-08-21

Applicant: James M. Wilson ,  Walter J. Symes, Jr.

Inventor： James M. Wilson ,  Walter J. Symes, Jr.

IPC: C04B35/46 ,  H01B3/12 ,  H01G4/12

CPC classification number: H01G4/1227

Abstract: Multi-layer ceramic capacitors are made from novel low temperature fired ceramic dielectric compositions which may have dielectric constants of over 3200 at room temperature, and which do not vary in value by more than 15 percent over a temperature range of -55.degree. C. to +125.degree. C., with the value at +25.degree. C. being the reference point. The compositions comprise a base ceramic formulation or host material, a ceramic sintering aid, and a low melting point glass formulation, each derived from metal oxides or precursors thereof. The host material is made from 97.89 to 98.19 weight percent barium titanate and from about 1.81 to 2.11 weight percent neodymium oxide, the sintering aid from Bi.sub.2 O.sub.3 .multidot.2TiO.sub.2, and the the glass formulation from 86.0 wt. % PbO, 9.0 wt. % B.sub.2 O.sub.3, 1.58 wt. % SiO.sub.2, 0.13 wt. % TiO.sub.2 and 3.29 wt. % Al.sub.2 O.sub.3. Manganese dioxide or a precursor is added in an amount of about 0.205 wt. % as the dioxide based on the combined weight of the ceramic formulation, the Bi.sub.2 O.sub.3 .multidot.2TiO.sub.2 and the glass formulation. The mixture, when made into a multi-layer capacitor is fired at or below 1100.degree. C.

Abstract translation: 多层陶瓷电容器由新型低温烧制陶瓷电介质组合物制成，其在室温下可以具有超过3200的介电常数，并且在-55℃至-55℃的温度范围内其值不会变化超过15％ + 125℃，值为+ 25℃为参考点。 组合物包含基础陶瓷制剂或主体材料，陶瓷烧结助剂和低熔点玻璃制剂，各自衍生自金属氧化物或其前体。 主体材料由97.89至98.19重量％的钛酸钡和约1.81至2.11重量百分比的氧化钕，来自Bi 2 O 3 x 2 TiO 2的烧结助剂和86.0重量％的玻璃制剂制成。 ％PbO，9.0wt。 ％B2O3，1.58wt。 ％SiO 2，0.13重量％ ％TiO 2和3.29重量％ ％Al2O3。 加入二氧化锰或前体的量为约0.205wt。 基于陶瓷配方，Bi2O3x2TiO2和玻璃配方的组合重量，作为二氧化物。 制成多层电容器时的混合物在1100℃以下烧成。

公开(公告)号：US4379319A

公开(公告)日：1983-04-05

申请号：US349849

申请日：1982-02-18

Applicant: James M. Wilson

Inventor： James M. Wilson

IPC: C04B35/497 ,  H01G4/12 ,  C04B35/00

CPC classification number: C04B35/497 ,  H01G4/1254

Abstract: A multi-layer ceramic capacitor is prepared from a novel ceramic composition produced from the oxides of lead (PbO), iron (Fe.sub.2 O.sub.3), niobium (Nb.sub.2 O.sub.5), nickel (NiO), and tungsten (WO.sub.3), or the synthesized compounds Pb(Fe.sub.1/2 Nb.sub.1/2)O.sub.3, Pb(Fe.sub.2/3 W.sub.1/3)O.sub.3 and Pb(Ni.sub.1/3 Nb.sub.2/3)O.sub.3, together with a small quantity of a Mn(NO.sub.3).sub.2 solution to improve certain electric properties of the capacitor. When used in the correct proportions the oxides disclosed herein are capable of producing monolithic ceramic capacitors having Z5U ratings. In particular, the lead, nickel, niobate compound tends to flatten and to lower the curie peak for the ceramic composition.

Abstract translation: 由Pb（PbO），铁（Fe2O3），铌（Nb2O5），镍（NiO）和钨（WO3））的氧化物或合成化合物Pb（ Fe1 / 2Nb1 / 2）O3，Pb（Fe2 / 3W1 / 3）O3和Pb（Ni1 / 3Nb2 / 3）O3，以及少量的Mn（NO3）2溶液，以提高电容器的某些电性能​​。 当以正确的比例使用时，本文公开的氧化物能够制造具有Z5U等级的单片陶瓷电容器。 特别地，铅，镍，铌酸盐化合物倾向于变平且降低陶瓷组合物的居里峰。

公开(公告)号：US09884071B2

公开(公告)日：2018-02-06

申请号：US13985630

申请日：2012-02-17

Applicant: James M. Wilson ,  Christie L. Bell ,  Luc H. Vandenberghe

Inventor： James M. Wilson ,  Christie L. Bell ,  Luc H. Vandenberghe

IPC: C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/85 ,  A61K31/7088 ,  A61K31/685 ,  A61K38/47

CPC classification number: A61K31/7088 ,  A61K31/685 ,  A61K38/47 ,  C12N15/86 ,  C12N2750/14043 ,  C12N2750/14045 ,  C12N2810/6027

Abstract: A method of altering the targeting and/or cellular uptake efficiency of an adeno-associated virus (AAV) viral vector having a capsid containing an AAV9 cell surface binding domain is described. The method involves modifying a clade F cell surface receptor which comprises a glycan having a terminal sialic acid residue and a penultimate β-galactose residue. The modification may involve retargeting the vector by temporarily functionally ablate AAV9 binding in a subset of cells, thereby redirecting the vector to another subset of cells. Alternatively, the modification may involve increasing cellular update efficiency by treating the cells with a neuraminidase to expose cell surface β-galactose. Also provided are compositions containing the AAV9 vector and a neuraminidase. Also provided is a method for purifying AAV9 using β-galactose linked to solid support. Also provided are mutant vectors which have been modified to alter their targeting specificity, including mutant AAV9 in which the galactose binding domain is mutated and AAV in which an AAV9 galactose binding domain is engineered.

公开(公告)号：US08961032B2

公开(公告)日：2015-02-24

申请号：US13420079

申请日：2012-03-14

Applicant: Terry L. Cooke ,  Christopher S. Houser ,  Ronald A. Leonard ,  James M. Wilson

Inventor： Terry L. Cooke ,  Christopher S. Houser ,  Ronald A. Leonard ,  James M. Wilson

IPC: G02B6/38

CPC classification number: G02B6/4465 ,  G02B6/3849

Abstract: An interconnect assembly includes an optical fiber cable, legs, connectors, and covers. The optical fiber cable includes a jacket and sub-units. The jacket has an interior defining a passage and the sub-units extend through the passage and include optical fibers extending lengthwise through the sub-units. The legs of the interconnect assembly extend from the passage on an end of the jacket, where the legs are continuations of or extensions from the sub-units such that the optical fibers further extend through the legs. The connectors are attached to the optical fibers on distal ends of the legs and the covers are attached to the connectors. The covers each include an end having a track for wrapping one or more of the legs over in order to package the legs and connectors, such as for placement of the legs and connectors within a pulling grip for installation of the interconnect assembly through a duct.

Abstract translation: 互连组件包括光纤电缆，支腿，连接器和盖。 光缆包括夹套和子单元。 护套具有限定通道的内部，并且子单元延伸穿过通道并且包括纵向延伸穿过子单元的光纤。 互连组件的腿部从夹套的端部上的通道延伸，其中腿部是子单元的延伸或延伸，使得光纤进一步延伸穿过腿部。 连接器附接到腿的远端处的光纤，并且盖连接到连接器。 盖子包括具有用于将一个或多个腿部包裹以覆盖腿部和连接器的轨道的端部，例如用于将腿部和连接器放置在牵引夹具内，以通过导管安装互连组件。

公开(公告)号：US20130023033A1

公开(公告)日：2013-01-24

申请号：US13638015

申请日：2011-03-28

Applicant: James M. Wilson ,  Shu-Jen Chen ,  Anna P. Tretiakova

Inventor： James M. Wilson ,  Shu-Jen Chen ,  Anna P. Tretiakova

IPC: C12N7/01 ,  C12N5/10 ,  C12N1/21 ,  C12N15/63

CPC classification number: C12N15/86 ,  A61K48/0066 ,  C07K2319/715 ,  C07K2319/80 ,  C12N9/22 ,  C12N2750/14143 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/002 ,  C12N2830/003 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/20 ,  C12N2840/203

Abstract: The present invention relates to gene therapy systems designed for the delivery of a therapeutic product to a subject using replication-defective virus composition(s) engineered with a built-in safety mechanism for ablating the therapeutic gene product, either permanently or temporarily, in response to a pharmacological agent—preferably an oral formulation, e.g., a pill. The invention is based, in part, on the applicants' development of an integrated approach, referred to herein as “PITA” (Pharmacologically Induced Transgene Ablation), for ablating a transgene or negatively regulating transgene expression. In this approach, replication-deficient viruses are used to deliver a transgene encoding a therapeutic product (an RNA or a protein) so that it is expressed in the subject, but can be reversibly or irreversibly turned off by administering the pharmacological agent; e.g., by administration of a small molecule that induces expression of an ablator specific for the transgene or its RNA transcript.

Abstract translation: 本发明涉及基因治疗系统，其被设计用于使用使用内置安全机制工程改造的复制缺陷病毒组合物将治疗产品递送给受试者，所述安全机制永久地或临时地用于消融治疗性基因产物 药物剂 - 优选口服制剂，例如丸剂。 本发明部分地基于申请人开发综合方法，本文称为PITA（药理学诱导的转基因消融），用于消融转基因或负调节转基因表达。 在这种方法中，使用复制缺陷病毒递送编码治疗产物（RNA或蛋白质）的转基因，使其在受试者中表达，但是可以通过施用药理学试剂来逆转或不可逆地关闭; 例如通过施用诱导特异于转基因或其RNA转录物的消融体表达的小分子。

公开(公告)号：US08318480B2

公开(公告)日：2012-11-27

申请号：US11981191

申请日：2007-10-31

Applicant: Guangping Gao ,  James M. Wilson ,  Mauricio R. Alvira

Inventor： Guangping Gao ,  James M. Wilson ,  Mauricio R. Alvira

IPC: C12N15/63 ,  C12N15/85

CPC classification number: C12N15/86 ,  A61K9/0019 ,  A61K35/761 ,  A61K38/177 ,  A61K38/45 ,  A61K38/4846 ,  A61K48/00 ,  C07K14/005 ,  C07K14/705 ,  C07K14/755 ,  C12N7/00 ,  C12N9/1018 ,  C12N9/644 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12Y201/03003 ,  C12Y304/21021 ,  C12Y304/21022

Abstract: Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles.

Abstract translation: 提供血清型8腺相关病毒和含有这些序列的载体和宿主细胞的序列。 还描述了在生产rAAV颗粒中使用这种宿主细胞和载体的方法。

公开(公告)号：US20120189582A1

公开(公告)日：2012-07-26

申请号：US13321985

申请日：2010-05-27

Applicant: Soumitra Roy ,  James M. Wilson ,  Luc H. Vandenberghe

Inventor： Soumitra Roy ,  James M. Wilson ,  Luc H. Vandenberghe

IPC: A61K35/76 ,  A61K38/02 ,  C12N15/34 ,  C07K14/075 ,  C12N7/01 ,  C12N15/63

CPC classification number: C12N15/86 ,  A61K39/145 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/10043 ,  C12N2710/10321 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2810/6018

Abstract: Novel simian adenovirus 41 and two isolates thereof are described. Various uses of these isolates, including construction of a recombinant vector which comprises simian adenovirus 41 sequences and a heterologous gene under the control of regulatory sequences are provided. A cell line which expresses simian adenovirus 41 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.

Abstract translation: 描述了新型猿猴腺病毒41及其两个分离物。 提供这些分离物的各种用途，包括构建包含猿猴腺病毒41序列和在调控序列控制下的异源基因的重组载体。 还公开了表达猿腺病毒41基因的细胞系。 提供了使用载体和细胞系的方法。

公开(公告)号：US20110151434A1

公开(公告)日：2011-06-23

申请号：US12962793

申请日：2010-12-08

Applicant: GUANGPING GAO ,  James M. Wilson ,  Mauricio R. Alvira

Inventor： GUANGPING GAO ,  James M. Wilson ,  Mauricio R. Alvira

IPC: C12Q1/70 ,  C12Q1/68 ,  C12N15/63

CPC classification number: C12N15/86 ,  A61K38/177 ,  A61K38/4846 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/14121 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2750/14171 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2830/90 ,  C12Q1/701 ,  C12Q2600/158 ,  C12Y304/21022

Abstract: A method for detecting and isolating AAV sequences in a sample of DNA obtained from tissue or cells is provided, which sample contains DNA and proviral AAV. The method involves subjecting the sample containing DNA to amplification via polymerase chain reaction (PCR) using a first set of primers which specifically amplify a first AAV region. The first AAV region is characterized by having at least 250 nucleotides of AAV capsid nucleic acid sequence, a variable sequence flanked by a sequence of at least 18 nucleotides at the 5′ end of the first AAV region and a sequence of at least 18 nucleotides at the 3′ end of the first AAV region. Each of the 5′ and 3′ at least 18 nucleotides is the same over at least 9 consecutive nucleotides relative to corresponding sequences in an alignment of at least two AAV serotypes. Each of the sets of primers consist of a 5′ primer and a 3′ primer. The method is further useful for identifying AAV sequences in the sample by the presence of amplified proviral AAV sequences.

Abstract translation: 提供了从组织或细胞获得的DNA样品中检测和分离AAV序列的方法，该样品含有DNA和前病毒AAV。 该方法包括使用特异性扩增第一AAV区域的第一组引物通过聚合酶链反应（PCR）使含有DNA的样品进行扩增。 第一个AAV区的特征在于具有AAV衣壳核酸序列的至少250个核苷酸，在第一AAV区的5'末端侧接至少18个核苷酸的序列的可变序列和至少18个核苷酸的序列 第一个AAV区域的3'端。 相对于至少两种AAV血清型的比对中的相应序列，5'和3'至少18个核苷酸中的每一个在至少9个连续核苷酸上相同。 每组引物由5'引物和3'引物组成。 该方法进一步用于通过扩增的前病毒AAV序列的存在来鉴定样品中的AAV序列。

公开(公告)号：US07860713B2

公开(公告)日：2010-12-28

申请号：US12165755

申请日：2008-07-01

Applicant: Tirso M. Alonso ,  Ilana Bromberg ,  Dilek Z. Hakkani-Tur ,  Barbara B. Hollister ,  Mazin G. Rahim ,  Giuseppe Riccardi ,  Lawrence Lyon Rose ,  Daniel Leon Stern ,  Gokhan Tur ,  James M. Wilson

Inventor： Tirso M. Alonso ,  Ilana Bromberg ,  Dilek Z. Hakkani-Tur ,  Barbara B. Hollister ,  Mazin G. Rahim ,  Giuseppe Riccardi ,  Lawrence Lyon Rose ,  Daniel Leon Stern ,  Gokhan Tur ,  James M. Wilson

IPC: G10L15/14 ,  G10L15/22

CPC classification number: G10L15/19 ,  G10L15/063 ,  G10L15/183 ,  H04M3/4936

Abstract: Systems and methods for annotating speech data. The present invention reduces the time required to annotate speech data by selecting utterances for annotation that will be of greatest benefit. A selection module uses speech models, including speech recognition models and spoken language understanding models, to identify utterances that should be annotated based on criteria such as confidence scores generated by the models. These utterances are placed in an annotation list along with a type of annotation to be performed for the utterances and an order in which the annotation should proceed. The utterances in the annotation list can be annotated for speech recognition purposes, spoken language understanding purposes, labeling purposes, etc. The selection module can also select utterances for annotation based on previously annotated speech data and deficiencies in the various models.

Abstract translation: 用于注释语音数据的系统和方法。 本发明通过选择最有益的用于注释的话语来减少注释语音数据所需的时间。 选择模块使用包括语音识别模型和语言理解模型在内的语音模型来基于诸如由模型产生的置信度得分的标准来识别应当注释的话语。 这些话语被放置在注释列表中，以及要为语句执行的注释类型以及注释应该继续执行的顺序。 注释列表中的话语可以被注释用于语音识别目的，语言理解目的，标签目的等。选择模块还可以基于先前注释的语音数据和各种模型中的缺陷来选择用于注释的话语。

公开(公告)号：US07560966B2

公开(公告)日：2009-07-14

申请号：US12002878

申请日：2007-12-19

Applicant: Joseph O. Marsh ,  Joseph Natonio ,  James M. Wilson

Inventor： Joseph O. Marsh ,  Joseph Natonio ,  James M. Wilson

IPC: H03K3/289

CPC classification number: H03K3/356043

Abstract: A method of testing connectivity through a plurality of dual purpose current mode logic (“CML”) latch circuits connected in a series is provided. Each of the CML latch circuits are operable to latch at least one output signal at a timing in accordance with at least one clock signal and having a mode control device for operating the CML latch circuit as a buffer amplifier when the at least one clock signal is inactive. The method comprises the steps of activating the mode control devices of each of the CML latches to operate each of the CML latches as a buffer; inputting a first signal to a first CML latch of the series; latching an output signal of a second CML latch of the series, the second CML latch being connected at a point in the series downstream from the first CML latch; and determining whether the output signal changes in accordance with a change in the first signal.

Abstract translation: 提供了通过串联连接的多个双用途电流模式逻辑（“CML”）锁存电路来测试连接性的方法。 每个CML锁存电路可操作以根据至少一个时钟信号在定时锁存至少一个输出信号，并具有一个模式控制装置，用于当至少一个时钟信号为 不活动 该方法包括以下步骤：激活每个CML锁存器的模式控制装置，以操作每个CML锁存器作为缓冲器; 向所述系列的第一CML锁存器输入第一信号; 锁存串联的第二CML锁存器的输出信号，第二CML锁存器在第一CML锁存器下游的串联点连接; 以及确定所述输出信号是否根据所述第一信号的改变而改变。