公开(公告)号：EP2800968B1

公开(公告)日：2017-03-22

申请号：EP12818971.9

申请日：2012-12-21

Applicant: Life Technologies Corporation

Inventor： REARICK, Todd ,  DAVEY, Melville ,  BEAUCHEMIN, Mark

IPC: G01N27/414

CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

公开(公告)号：EP2800968A1

公开(公告)日：2014-11-12

申请号：EP12818971.9

申请日：2012-12-21

Applicant: Life Technologies Corporation

Inventor： REARICK, Todd ,  DAVEY, Melville ,  BEAUCHEMIN, Mark

IPC: G01N27/414

CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

Abstract translation: 提供了一种方法，计算机程序产品和系统来校准具有多个传感器的传感器阵列。 该方法可以包括将参考电极的电压从第一电压扫描到第二电压，其中参考电极与传感器阵列流体连通。 可以在第一和第二电压内的一个或多个电压下监视多个传感器中的每一个的输出电压。 可以在一个或多个电压中的每一个处计算多个传感器的总体平均增益。 此外，可以确定传感器阵列的采集窗口。 采集窗口可以包括在特定参考电极电压下提供最大总平均增益的传感器的最大分布。

公开(公告)号：EP2617061A1

公开(公告)日：2013-07-24

申请号：EP10857377.5

申请日：2010-09-15

Applicant: Life Technologies Corporation ,  Rothberg, Johnathan ,  Rearick, Todd

Inventor： ROTHBERG, Johnathan ,  REARICK, Todd

IPC: H01L31/0232

CPC classification number: G01N27/4145 ,  C12Q1/6874 ,  G01N27/4148

Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：EP4246138A3

公开(公告)日：2024-01-24

申请号：EP23187427.2

申请日：2015-09-14

Applicant: Life Technologies Corporation

Inventor： WONG, Chiu Tai Andrew ,  REARICK, Todd ,  DONOHUE, John

IPC: G01N27/414 ,  G16B30/00

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：EP2699903B1

公开(公告)日：2018-07-18

申请号：EP12719172.4

申请日：2012-04-20

Applicant: Life Technologies Corporation

Inventor： REARICK, Todd ,  REED, Jessica ,  GIOIA, Jason ,  DRESSMAN, Devin ,  HAPSHE, Nicholas ,  REED, Brian

IPC: G01N33/50 ,  G01N33/543

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0893 ,  C12Q1/6837 ,  G01N33/50 ,  G01N33/543

Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.

公开(公告)号：EP2588838B1

公开(公告)日：2018-03-21

申请号：EP11834771.5

申请日：2011-06-28

Applicant: Life Technologies Corporation

Inventor： LIGHT, David ,  HINZ, Wolfgang ,  REARICK, Todd ,  BALL, James

IPC: G01N27/414 ,  B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2400/0415 ,  G01N27/4145

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

公开(公告)号：EP2588838A1

公开(公告)日：2013-05-08

申请号：EP11834771.5

申请日：2011-06-28

Applicant: Life Technologies Corporation

Inventor： LIGHT, David ,  HINZ, Wolfgang ,  REARICK, Todd ,  BALL, James

IPC: G01F1/64

CPC classification number: B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2400/0415 ,  G01N27/4145

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

公开(公告)号：EP2436075A1

公开(公告)日：2012-04-04

申请号：EP10780935.2

申请日：2010-05-27

Applicant: Life Technologies Corporation

Inventor： NOBILE, John ,  ROTH, George, Thomas ,  REARICK, Todd ,  SCHULTZ, Jonathan ,  ROTHBERG, Jonathan, M. ,  MARRAN, David

IPC: H01M8/04 ,  H01M8/10

CPC classification number: G01N27/301 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00722 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

Abstract: The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors under minimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles. Such detection of analyte- or particle-containing microwells may be used as a step in additional noise reduction methods.

公开(公告)号：EP4220146A1

公开(公告)日：2023-08-02

申请号：EP22188998.3

申请日：2010-05-27

Applicant: Life Technologies Corporation

Inventor： ROTHBERG, Jonathan M. ,  HINZ, Wolfgang ,  DAVIDSON, John F. ,  VAN OIJEN, Antoine M ,  LEAMON, John H. ,  HUBER, Martin ,  BUSTILLO, James M. ,  MILGREW, Mark, James ,  SCHULTZ, Jonathan ,  MARRAN, David ,  REARICK, Todd ,  JOHNSON, Kim L.

IPC: G01N27/416 ,  C12M1/34 ,  G01N27/414 ,  C12Q1/00

Abstract: An apparatus may include an array of sensors, a plurality of microwells, a row addressing circuit, a column addressing circuit, and analog-to-digital converters. Each sensor includes a two-transistor circuit with a chemically-sensitive field effect transistor (chemFET) and a row select transistor and each chemFETs has a floating gate structure and a passivation material located over at least a subset of the floating gate structures. The microwells define a plurality of cavities disposed over at least a portion of the floating gate structures and are of a size and shape capable of retaining analyte therein. The column addressing circuit is suitable for supplying output from each sensor in the array of sensors. The analog-to-digital converters are used for each column in the sensor array to convert an analog output of the column addressing circuit to digital form.

公开(公告)号：EP3194945A1

公开(公告)日：2017-07-26

申请号：EP15841566.1

申请日：2015-09-14

Applicant: Life Technologies Corporation

Inventor： WONG, Chiu, Tai Andrew ,  REARICK, Todd ,  DONOHUE, John

IPC: G01N27/26

CPC classification number: G01N27/4145 ,  G06F19/22

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

Abstract translation: 一种用于校正核酸掺入信号以便核酸测序合成中产生的流体潜在影响或干扰的方法包括：将多个模板多核苷酸链置于设置在传感器阵列上的多个限定空间中，模板多核苷酸链具有测序 引物和与其结合的聚合酶; 将所述模板多核苷酸链暴露于流经流体歧管的一系列核苷酸物质流，所述流体歧管包括用于流动核苷酸物质的通道和用于使溶液流动的分支通道，所述分支通道包括参考电极和感测电极; 获得与所述多个限定空间对应的多个核苷酸掺入信号，所述核苷酸掺入信号具有与核苷酸掺入数目相关的信号强度; 以及修正所述多个核苷酸掺入信号中的至少一些以发现流体电位效应或干扰。